FastQCFastQC Report
Mon 15 Aug 2016
SRR2921628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587857
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.22306794629491195No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.18213227009737024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.130049494381421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.054.2077871
GTATCAA52800.045.9774321
GTACATG97750.034.9030531
TCAACGC69250.034.526934
TATCAAC71050.033.9050452
CAACGCA70600.033.8442765
TACATGG99200.033.780942
ATCAACG71600.033.3947683
ACATGGG99850.033.2624473
AACGCAG73100.032.7828036
ATGGGGG53250.032.226895
CATGGGG85250.032.0335044
TGGGGGG40200.028.4716476
TACCGTA1505.220083E-627.8182937
ACGCAGA86300.027.6985667
ATAAGTA6350.027.212595
CGCAGAG90950.026.2135728
GTATATA6150.025.2163621
ATAGGAC3800.025.1063423
GCAGAGT99100.023.9382279
Produced by FastQC (version 0.11.5)