##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921628_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587857
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.716963807194226	33.0	31.0	34.0	30.0	34.0
2	31.89980835805743	33.0	31.0	34.0	30.0	34.0
3	31.977765000248763	34.0	31.0	34.0	30.0	34.0
4	35.37375972773367	37.0	35.0	37.0	33.0	37.0
5	35.217019542691816	37.0	35.0	37.0	33.0	37.0
6	35.24271958998827	37.0	35.0	37.0	33.0	37.0
7	35.13953964368328	37.0	35.0	37.0	33.0	37.0
8	35.17631688495879	37.0	36.0	37.0	33.0	37.0
9	36.88025307064805	39.0	38.0	39.0	34.0	39.0
10-11	36.83745293184462	39.0	38.0	39.0	33.5	39.0
12-13	36.75364689641447	39.0	38.0	39.0	33.0	39.0
14-15	38.17104783365252	41.0	39.0	41.0	33.0	41.0
16-17	38.178818999443905	41.0	39.0	41.0	34.0	41.0
18-19	38.20089340538852	41.0	39.0	41.0	34.0	41.0
20-21	38.149319806506504	41.0	39.0	41.0	34.0	41.0
22-23	38.151989442374216	41.0	39.0	41.0	34.0	41.0
24-25	38.11080027987407	41.0	39.0	41.0	34.0	41.0
26-27	38.03428173947654	41.0	39.0	41.0	33.0	41.0
28-29	37.96132712202673	41.0	38.5	41.0	33.0	41.0
30-31	37.83545716018508	41.0	38.0	41.0	33.0	41.0
32-33	37.69425174937038	41.0	38.0	41.0	33.0	41.0
34-35	37.55996320827379	40.5	38.0	41.0	33.0	41.0
36-37	37.48684988635627	40.0	38.0	41.0	32.5	41.0
38-39	37.40373660852331	40.0	38.0	41.0	32.0	41.0
40-41	37.27712728539157	40.0	38.0	41.0	32.0	41.0
42-43	37.111338741460976	40.0	37.5	41.0	31.5	41.0
44-45	36.93745438033777	40.0	37.0	41.0	31.0	41.0
46-47	36.697164795066556	40.0	37.0	41.0	30.0	41.0
48-49	36.471505305578525	40.0	36.5	41.0	30.0	41.0
50-51	36.216579011837965	40.0	36.0	41.0	29.0	41.0
52-53	36.09791687790525	40.0	36.0	41.0	29.0	41.0
54-55	35.97377786538712	40.0	35.0	41.0	29.0	41.0
56-57	35.785685675725205	39.0	35.0	41.0	28.0	41.0
58-59	35.5969882678352	39.0	35.0	41.0	28.0	41.0
60-61	35.344777583875626	39.0	35.0	41.0	28.0	41.0
62-63	34.99519352183478	38.0	35.0	41.0	28.0	41.0
64-65	34.70665336991934	37.0	35.0	40.0	28.0	41.0
66-67	34.40766013564193	37.0	35.0	39.5	28.0	41.0
68-69	34.03558003019164	36.0	35.0	39.0	26.5	41.0
70-71	33.66158130108693	36.0	34.5	39.0	26.0	41.0
72-73	33.337316899443714	35.0	34.0	37.5	26.0	40.0
74-75	33.017049394246456	35.0	34.0	37.0	26.0	39.0
76-77	32.68865300842582	35.0	34.0	37.0	26.0	39.0
78-79	32.42699216617113	35.0	34.0	36.0	26.0	38.5
80-81	32.1745475190776	35.0	34.0	36.0	25.0	37.0
82-83	31.94938492572064	35.0	34.0	35.5	25.0	37.0
84-85	31.734699661241535	35.0	34.0	35.0	24.5	36.5
86-87	31.606043869189733	35.0	33.5	35.0	24.0	36.0
88-89	31.508646559482372	35.0	33.0	35.0	24.0	36.0
90-91	31.3966260815678	35.0	33.0	35.0	24.0	36.0
92-93	31.31263331647623	35.0	33.0	35.0	23.0	35.0
94-95	31.246242577259792	35.0	33.0	35.0	23.0	35.0
96-97	31.178770821301917	35.0	33.0	35.0	23.0	35.0
98-99	31.10614179992279	35.0	33.0	35.0	21.5	35.0
100-101	31.039466400311866	35.0	33.0	35.0	20.0	35.0
102-103	30.357670747428767	34.0	31.5	35.0	19.5	35.0
104-105	30.866746186841763	35.0	33.0	35.0	19.5	35.0
106-107	30.962063019528834	35.0	33.0	35.0	20.0	35.0
108-109	30.95390422437285	35.0	33.0	35.0	19.5	35.0
110-111	30.891225406318075	35.0	33.0	35.0	19.0	35.0
112-113	30.857467328607047	35.0	33.0	35.0	19.0	35.0
114-115	30.733121118589395	35.0	33.0	35.0	18.0	35.0
116-117	30.91228870106061	35.0	33.0	35.0	18.5	35.0
118-119	30.894817354459498	35.0	33.0	35.0	16.5	35.0
120-121	30.82095176076939	35.0	33.0	35.0	9.0	35.0
122-123	30.69177482607061	35.0	33.0	35.0	2.0	35.0
124-125	30.057056775263767	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25570.0
3	6906.0
4	3781.0
5	2964.0
6	3133.0
7	3389.0
8	4000.0
9	4131.0
10	4584.0
11	5076.0
12	5814.0
13	7610.0
14	10933.0
15	10408.0
16	5671.0
17	4769.0
18	4192.0
19	3629.0
20	3460.0
21	3329.0
22	3735.0
23	4603.0
24	6099.0
25	7907.0
26	9645.0
27	11106.0
28	13310.0
29	15919.0
30	19441.0
31	24105.0
32	32167.0
33	43301.0
34	68576.0
35	121147.0
36	272262.0
37	589558.0
38	221167.0
39	460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55783700905897	17.970414875838355	11.831026017850855	26.640722097251818
2	15.720004232207096	19.715724160864177	38.15340994971735	26.410861657211377
3	18.62749606733751	24.203592535217567	28.544294347738713	28.62461704970621
4	13.346323776169077	15.355195462218378	34.312936361548964	36.98554440006359
5	14.462877595784004	37.68014422877041	32.08359422933735	15.773383946108229
6	33.951880851664555	33.94576764259533	16.95060527072063	15.151746235019486
7	30.07039715285987	32.19856886847856	19.09362090389642	18.637413074765156
8	28.286388452220812	34.859952934356976	18.21522293174222	18.63843568168
9	28.493804325160006	15.760871961933248	18.252182771305293	37.49314094160145
10-11	27.051802846440438	25.278260222935877	25.951523294328798	21.71841363629489
12-13	26.901663036529587	22.525838142621694	26.669109513143635	23.90338930770508
14-15	24.409591968295636	23.66232258771916	24.47244993575569	27.45563550822951
16-17	24.344427102662998	26.81275349753347	24.944503848972335	23.8983155508312
18-19	24.22421489066399	25.86071117388864	27.151140044214472	22.763933891232895
20-21	25.08398578619704	24.757397143002926	26.845088823669847	23.313528247130183
22-23	24.836953252952256	24.29157995139851	26.899824066017914	23.97164272963132
24-25	24.232113509178046	24.57306049596812	26.853724075102182	24.341101919751654
26-27	23.89331568214253	24.831087676958017	27.08136258459787	24.19423405630159
28-29	24.10014580059643	25.560450564780496	26.68513054349298	23.654273091130097
30-31	24.839158355216068	24.959039739718524	26.748726791678777	23.453075113386628
32-33	24.391569632411407	24.790350897911303	26.841351945015234	23.976727524662053
34-35	24.3404031268658	25.163718330154904	26.844578747288683	23.65129979569061
36-37	24.31006993222806	25.242867035553108	26.816790914699183	23.63027211751965
38-39	24.50384866817565	25.27597551866645	26.63459758083715	23.58557823232075
40-41	24.503127114750516	24.951544757843454	26.75022224069636	23.795105886709674
42-43	24.325732020990102	24.948828438917847	26.489391584309317	24.236047955782734
44-45	24.12927983433478	25.014824152330878	26.77479664223109	24.08109937110325
46-47	24.099734157292474	25.104406716039556	26.30668209280751	24.48917703386046
48-49	24.10864029897464	25.41076578241836	26.247758729382987	24.232835189224016
50-51	24.267026151107533	25.460699653205836	26.086554003436767	24.18572019224986
52-53	24.32447581338434	25.465522051641194	25.942364506269833	24.267637628704637
54-55	23.971618118075405	24.97188673321478	26.424927736859193	24.631567411850618
56-57	24.25283859928217	25.278790157465192	25.94239480855475	24.52597643469789
58-59	24.156025402999287	25.424474948420627	26.230253097911383	24.189246550668706
60-61	24.591516414129885	25.23386116297179	26.31758766310137	23.857034759796946
62-63	24.517802846137762	25.27616202177051	26.278815948246542	23.92721918384518
64-65	24.208004877013483	24.927370663731054	26.326741010053173	24.537883449202287
66-67	24.142447142372195	25.32077342088408	26.199062478153778	24.33771695858995
68-69	24.08334296376512	25.12862530650752	26.327858104850684	24.46017362487668
70-71	24.137375091081882	25.109156555029827	26.393592118181925	24.359876235706366
72-73	24.201773818464652	25.65786055387348	25.7857089999323	24.35465662772957
74-75	24.08684167765681	25.49928012969002	25.90122346462307	24.5126547280301
76-77	23.956709321464682	25.041023110551574	26.093154501646747	24.909113066336992
78-79	23.87447074760699	25.111707443951047	26.33313663166553	24.68068517677644
80-81	24.11851319104932	25.410191219990214	25.808778750227507	24.66251683873296
82-83	24.19854424484197	25.511173868709957	25.86352405611151	24.42675783033657
84-85	24.185750656163847	25.531772185580614	25.67539609938737	24.60708105886817
86-87	23.847707948511736	25.737393228735332	25.767780096066584	24.647118726686344
88-89	23.827249324946663	25.297298999425323	25.959520416919236	24.915931258708778
90-91	24.154678971825625	25.005833383453723	26.07807381520912	24.761413829511536
92-93	23.970042642379006	25.530573596992678	25.887879072233833	24.611504688394483
94-95	23.942220973820515	25.317324260867814	25.82273801366412	24.917716751647554
96-97	23.645079915494918	25.399640453485834	25.893804356691053	25.0614752743282
98-99	23.69768623803175	25.343688655141207	25.660186403349943	25.2984387034771
100-101	23.60839168766457	25.49096045802613	25.820335206507888	25.080312647801406
102-103	23.439309652930806	25.360190800200776	25.947403987431976	25.25309555943644
104-105	23.810865839933946	25.328981136210626	25.812242538213454	25.047910485641967
106-107	23.74615556352582	25.26267495000304	25.853884490723754	25.137284995747383
108-109	23.680892496359778	25.204967074270566	25.984843331972975	25.129297097396673
110-111	23.537580379587197	25.653357214584567	25.7772663965541	25.03179600927414
112-113	23.523745586020873	25.69258615249648	25.764223766305822	25.019444495176817
114-115	23.582948590458713	25.705904247044913	25.671297856167147	25.03984930632922
116-117	23.362211374412954	25.433816152515355	25.88115707819555	25.32281539487614
118-119	23.432258430203724	25.354078790541106	25.924704822814963	25.288957956440207
120-121	23.37833446277015	25.434528772927777	25.999362644947894	25.187774119354177
122-123	23.308152295421124	25.58719892180233	25.752423897949093	25.35222488482745
124-125	23.14907605510727	25.45932445267704	25.734274463915252	25.65732502830043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	16.0
2	30.0
3	71.5
4	75.5
5	75.0
6	175.5
7	530.5
8	926.5
9	1058.0
10	986.5
11	843.0
12	825.5
13	900.5
14	996.0
15	1093.0
16	1118.5
17	1188.5
18	1287.5
19	1354.5
20	1454.5
21	1568.0
22	1701.5
23	1949.0
24	2222.5
25	2617.5
26	3254.5
27	4110.0
28	5178.5
29	6433.0
30	7960.5
31	9604.5
32	11898.5
33	14397.0
34	16550.5
35	19037.0
36	22015.5
37	24882.0
38	27624.0
39	30729.0
40	33869.5
41	36485.0
42	39345.5
43	44102.0
44	49468.0
45	54407.5
46	57963.0
47	61705.0
48	67191.5
49	72126.5
50	75107.0
51	75252.5
52	73481.5
53	71320.0
54	70343.5
55	68329.0
56	63898.5
57	58649.0
58	53572.0
59	48327.0
60	40679.5
61	33137.0
62	27083.0
63	20829.5
64	15382.5
65	11532.5
66	8682.5
67	6711.5
68	5210.0
69	3780.0
70	2858.0
71	2259.0
72	1738.5
73	1365.5
74	1015.0
75	732.0
76	499.5
77	276.0
78	161.5
79	92.0
80	50.0
81	26.5
82	15.5
83	13.0
84	12.0
85	8.5
86	3.0
87	1.5
88	2.0
89	1.5
90	1.5
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023301846450908364
2	0.002078272791567503
3	0.03211876132422504
4	0.16304994719297772
5	0.023868648121335864
6	0.07110212065696092
7	0.05422402647089757
8	0.04484030992715339
9	0.03400810022565004
10-11	0.06505623617240092
12-13	0.05734143565824882
14-15	0.0595771533582684
16-17	0.05101215033847507
18-19	0.03740891024821504
20-21	0.024309493865001697
22-23	0.00286549733382792
24-25	0.004628880308491256
26-27	0.003211876132422504
28-29	0.004534413363420006
30-31	0.0023616736267812527
32-33	0.009100315708530428
34-35	0.004345479473277505
36-37	0.0066441751366779255
38-39	0.0022042287183291695
40-41	0.0032433651141129207
42-43	0.003841655766230838
44-45	0.004817814198633756
46-47	0.004786325216943339
48-49	0.0018893389014250023
50-51	0.001731893992972919
52-53	0.0010076474140933347
54-55	0.00525865994229959
56-57	8.816914873316678E-4
58-59	8.187135239508343E-4
60-61	1.5744490845208354E-4
62-63	0.0013225372309975017
64-65	0.0
66-67	0.0034008100225650043
68-69	5.35312688737084E-4
70-71	0.0
72-73	0.0024876295535429197
74-75	0.004471435400039172
76-77	0.002046783809877086
78-79	2.5191185352333367E-4
80-81	0.0
82-83	0.005668016704275008
84-85	0.0018578499197345855
86-87	0.0
88-89	0.0012280702859262516
90-91	8.502025056412511E-4
92-93	0.0
94-95	0.0030859202056608376
96-97	0.0014799821394495853
98-99	6.297796338083342E-4
100-101	0.0
102-103	0.0011336033408550015
104-105	0.0
106-107	6.612686154987508E-4
108-109	0.0025820964986141702
110-111	3.148898169041671E-5
112-113	2.5191185352333367E-4
114-115	0.0
116-117	0.0018893389014250023
118-119	0.0024876295535429197
120-121	0.002834008352137504
122-123	0.0013855151943783352
124-125	0.1102429248981489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1587857.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.13228835418312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.02371443773572	49.5316507453465
2	10.469945734744764	12.801034834181332
3	3.283013519376089	6.020943874115418
4	1.5908948853140672	3.890201794808585
5	0.9002539519562794	2.7517292091492096
6	0.5746259543181965	2.1076919721106586
7	0.410656228290239	1.75730484735961
8	0.2922604333327211	1.4293239268011535
9	0.21999553002736913	1.210394716043803
>10	1.1501640977147154	12.28233048156358
>50	0.05685334527426464	2.369592110370602
>100	0.026173658460140185	2.7700360851637127
>500	0.001034445325339109	0.44412054363492137
>1k	4.137781301356436E-4	0.6336448593509771
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3542	0.22306794629491195	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2892	0.18213227009737024	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2065	0.130049494381421	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.297796338083342E-5
7	0.0	0.0	0.0	0.0	6.297796338083342E-5
8	0.0	0.0	0.0	0.0	6.297796338083342E-5
9	0.0	0.0	0.0	0.0	6.297796338083342E-5
10-11	0.0	0.0	0.0	6.297796338083342E-5	6.297796338083342E-5
12-13	0.0	0.0	0.0	6.297796338083342E-5	9.446694507125013E-5
14-15	0.0	0.0	0.0	6.297796338083342E-5	1.8893389014250023E-4
16-17	0.0	0.0	0.0	1.2595592676166683E-4	1.8893389014250023E-4
18-19	0.0	0.0	0.0	1.8893389014250023E-4	1.8893389014250023E-4
20-21	0.0	0.0	0.0	1.8893389014250023E-4	2.5191185352333367E-4
22-23	0.0	0.0	0.0	3.4637879859458374E-4	2.834008352137504E-4
24-25	0.0	0.0	0.0	8.187135239508343E-4	3.148898169041671E-4
26-27	0.0	0.0	0.0	0.0011021143591645848	3.148898169041671E-4
28-29	0.0	0.0	0.0	0.0022986956634004196	3.148898169041671E-4
30-31	0.0	0.0	0.0	0.00925776061698251	3.148898169041671E-4
32-33	0.0	0.0	0.0	0.024939273498810033	3.7786778028500045E-4
34-35	0.0	0.0	0.0	0.043517772696155886	5.038237070466673E-4
36-37	0.0	0.0	0.0	0.06874044703017967	5.668016704275008E-4
38-39	0.0	0.0	0.0	0.11521818400523473	5.668016704275008E-4
40-41	0.0	0.0	0.0	0.17328386624236314	5.668016704275008E-4
42-43	0.0	0.0	0.0	0.2233513471301257	5.668016704275008E-4
44-45	0.0	0.0	0.0	0.273324361072817	5.982906521179174E-4
46-47	0.0	6.297796338083342E-5	0.0	0.3311381314564221	6.927575971891676E-4
48-49	0.0	6.297796338083342E-5	0.0	0.39950071070631676	7.557355605700009E-4
50-51	0.0	6.297796338083342E-5	0.0	0.47139005590553806	7.557355605700009E-4
52-53	0.0	6.297796338083342E-5	0.0	0.5478138144681794	8.816914873316677E-4
54-55	0.0	6.297796338083342E-5	0.0	0.6353216945858475	9.446694507125012E-4
56-57	0.0	6.297796338083342E-5	0.0	0.7199325883879972	9.446694507125012E-4
58-59	0.0	6.297796338083342E-5	0.0	0.8218939111015664	9.446694507125012E-4
60-61	0.0	6.297796338083342E-5	0.0	0.9304049420067424	9.446694507125012E-4
62-63	0.0	6.297796338083342E-5	0.0	1.0545030188486746	0.0010076474140933347
64-65	0.0	6.297796338083342E-5	0.0	1.1639902081862536	0.0010391363957837512
66-67	0.0	6.297796338083342E-5	0.0	1.2877733952112815	0.001070625377474168
68-69	0.0	6.297796338083342E-5	0.0	1.431142728847749	0.001070625377474168
70-71	0.0	6.297796338083342E-5	0.0	1.5852498052406483	0.0011336033408550015
72-73	0.0	6.297796338083342E-5	0.0	1.7201486028023933	0.0012280702859262516
74-75	0.0	6.297796338083342E-5	0.0	1.8608098840134848	0.0012595592676166683
76-77	0.0	6.297796338083342E-5	0.0	2.028740623368477	0.0012595592676166683
78-79	0.0	6.297796338083342E-5	0.0	2.224224221702584	0.0012595592676166683
80-81	0.0	6.297796338083342E-5	0.0	2.411268772943659	0.001385515194378335
82-83	0.0	6.297796338083342E-5	0.0	2.610436582135545	0.0015744490845208353
84-85	0.0	6.297796338083342E-5	0.0	2.812721800514782	0.0015744490845208353
86-87	0.0	6.297796338083342E-5	0.0	3.0454883531703425	0.001605938066211252
88-89	0.0	6.297796338083342E-5	0.0	3.3022809988556903	0.0016374270479016686
90-91	0.0	6.297796338083342E-5	0.0	3.5749755802946988	0.0016374270479016686
92-93	0.0	6.297796338083342E-5	0.0	3.834633723313875	0.0016374270479016686
94-95	0.0	6.297796338083342E-5	0.0	4.09649609505138	0.0017004050112825022
96-97	0.0	6.297796338083342E-5	0.0	4.397782671865287	0.0017948719563537522
98-99	6.297796338083342E-5	6.297796338083342E-5	0.0	4.749703531237385	0.001920827883115419
100-101	6.297796338083342E-5	6.297796338083342E-5	0.0	5.115479542553265	0.0019523168648058358
102-103	6.297796338083342E-5	6.297796338083342E-5	0.0	5.4961498422087125	0.0019838058464962524
104-105	6.297796338083342E-5	6.297796338083342E-5	0.0	5.882015823843079	0.002046783809877086
106-107	6.297796338083342E-5	6.297796338083342E-5	0.0	6.303653288677759	0.0020782727915675024
108-109	6.297796338083342E-5	6.297796338083342E-5	0.0	6.752056387949293	0.0021097617732579194
110-111	6.297796338083342E-5	6.297796338083342E-5	0.0	7.234845455226761	0.0021727397366387525
112-113	6.297796338083342E-5	6.297796338083342E-5	0.0	7.715083914987307	0.0022042287183291695
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3345	0.0	54.207787	1
GTATCAA	5280	0.0	45.977432	1
GTACATG	9775	0.0	34.903053	1
TCAACGC	6925	0.0	34.52693	4
TATCAAC	7105	0.0	33.905045	2
CAACGCA	7060	0.0	33.844276	5
TACATGG	9920	0.0	33.78094	2
ATCAACG	7160	0.0	33.394768	3
ACATGGG	9985	0.0	33.262447	3
AACGCAG	7310	0.0	32.782803	6
ATGGGGG	5325	0.0	32.22689	5
CATGGGG	8525	0.0	32.033504	4
TGGGGGG	4020	0.0	28.471647	6
TACCGTA	150	5.220083E-6	27.818293	7
ACGCAGA	8630	0.0	27.698566	7
ATAAGTA	635	0.0	27.21259	5
CGCAGAG	9095	0.0	26.213572	8
GTATATA	615	0.0	25.216362	1
ATAGGAC	380	0.0	25.106342	3
GCAGAGT	9910	0.0	23.938227	9
>>END_MODULE