FastQCFastQC Report
Mon 15 Aug 2016
SRR2921627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247486
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.048.552651
GTATCAA43100.046.550411
TCAACGC52400.037.596024
CAACGCA53550.036.6750375
GTACATG119100.035.2411
ATCAACG56150.035.085153
AACGCAG56350.034.9551776
TATCAAC58800.033.7063752
TACATGG125300.033.2975352
ACATGGG126100.032.1423153
CATGGGG109900.031.1939164
ATGGGGG65950.028.7867095
ACGCAGA69500.028.3312637
CGCAGAG74250.026.6766838
GCAGAGT83150.023.3926379
ATGGGGA41250.021.9298535
GTATAGG6700.021.3258971
AGAGTAC76250.020.9846310-11
GTAAGTA9700.020.2482432
GAGTACA84350.019.4802671
Produced by FastQC (version 0.11.5)