FastQCFastQC Report
Mon 15 Aug 2016
SRR2921627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247486
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.14086850819092978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26540.11808749865405167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30950.058.769821
GTATCAA55700.055.5683361
TCAACGC76200.039.9820374
ATCAACG78750.038.7656823
CAACGCA78850.038.608185
TATCAAC79500.038.4008262
AACGCAG81800.037.224136
GTACATG120250.033.9719661
TACATGG124800.032.297572
ACGCAGA96850.031.4375867
ACATGGG125100.031.4091473
CATGGGG108150.030.3204154
CGCAGAG104200.029.1589768
ATGGGGG64200.028.7664855
GCAGAGT113950.026.7163079
AGAGTAC105400.024.84208910-11
GAGTACT48350.022.03732112-13
CAGAGTA111500.021.77321610-11
ATGGGGA41750.020.975925
GTGGTAT18350.020.7997991
Produced by FastQC (version 0.11.5)