##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2247486
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65947819029796	33.0	31.0	34.0	30.0	34.0
2	31.849223977368492	33.0	31.0	34.0	30.0	34.0
3	31.928684761551352	34.0	31.0	34.0	30.0	34.0
4	35.31894703682248	37.0	35.0	37.0	33.0	37.0
5	35.14997690753135	37.0	35.0	37.0	33.0	37.0
6	35.168486922721655	37.0	35.0	37.0	33.0	37.0
7	35.05929380650202	37.0	35.0	37.0	33.0	37.0
8	35.09525176130129	37.0	35.0	37.0	33.0	37.0
9	36.80216917925184	39.0	38.0	39.0	33.0	39.0
10-11	36.76068549481509	39.0	38.0	39.0	33.0	39.0
12-13	36.68480382080244	39.0	38.0	39.0	33.0	39.0
14-15	38.114380467775995	41.0	39.0	41.0	33.0	41.0
16-17	38.11619493959028	41.0	39.0	41.0	33.0	41.0
18-19	38.12831247892089	41.0	39.0	41.0	33.5	41.0
20-21	38.06659730027239	41.0	39.0	41.0	33.0	41.0
22-23	38.06553166515832	41.0	39.0	41.0	33.5	41.0
24-25	38.026528975041444	41.0	39.0	41.0	33.0	41.0
26-27	37.9542978243246	41.0	39.0	41.0	33.0	41.0
28-29	37.88714278976599	41.0	38.5	41.0	33.0	41.0
30-31	37.774521843517604	41.0	38.0	41.0	33.0	41.0
32-33	37.661850841340055	41.0	38.0	41.0	33.0	41.0
34-35	37.527931653411855	41.0	38.0	41.0	32.0	41.0
36-37	37.45303508008504	40.0	38.0	41.0	32.0	41.0
38-39	37.36938828539978	40.0	38.0	41.0	32.0	41.0
40-41	37.25427566623329	40.0	38.0	41.0	32.0	41.0
42-43	37.091723374472636	40.0	37.5	41.0	31.0	41.0
44-45	36.93315464478978	40.0	37.0	41.0	31.0	41.0
46-47	36.72851488285133	40.0	37.0	41.0	30.5	41.0
48-49	36.53718265653268	40.0	36.5	41.0	30.0	41.0
50-51	36.29215332153348	40.0	36.0	41.0	30.0	41.0
52-53	36.1596483804571	40.0	36.0	41.0	29.0	41.0
54-55	36.02538236055753	40.0	35.0	41.0	29.0	41.0
56-57	35.82369545349782	39.0	35.0	41.0	29.0	41.0
58-59	35.62475294618075	39.0	35.0	41.0	29.0	41.0
60-61	35.365706393721695	38.5	35.0	41.0	28.0	41.0
62-63	35.01293645433164	37.5	35.0	40.5	28.0	41.0
64-65	34.71834173828002	37.0	35.0	40.0	28.0	41.0
66-67	34.413069313891164	36.5	35.0	39.5	28.0	41.0
68-69	34.04540006033408	36.0	35.0	39.0	27.5	41.0
70-71	33.67359729938251	35.5	34.5	39.0	26.0	41.0
72-73	33.35947565413088	35.0	34.0	37.5	26.0	40.0
74-75	33.040299472388256	35.0	34.0	37.0	26.0	39.0
76-77	32.72226456583044	35.0	34.0	36.5	26.0	39.0
78-79	32.47028502068534	35.0	34.0	36.0	26.0	38.0
80-81	32.22449750521249	35.0	34.0	36.0	26.0	37.0
82-83	32.00731506225178	35.0	34.0	35.0	25.0	37.0
84-85	31.804230593649972	35.0	34.0	35.0	25.0	36.0
86-87	31.682701249307	35.0	34.0	35.0	25.0	36.0
88-89	31.58675137464705	35.0	33.5	35.0	25.0	36.0
90-91	31.48528978600979	35.0	33.0	35.0	24.0	35.5
92-93	31.412687998946378	35.0	33.0	35.0	24.0	35.0
94-95	31.348359010912638	35.0	33.0	35.0	24.0	35.0
96-97	31.286070969963774	35.0	33.0	35.0	24.0	35.0
98-99	31.212194425237797	35.0	33.0	35.0	23.0	35.0
100-101	31.14454572798229	35.0	33.0	35.0	23.0	35.0
102-103	30.4514139353927	34.0	31.5	35.0	20.0	35.0
104-105	30.967566872496647	35.0	33.0	35.0	20.0	35.0
106-107	31.070914346073792	35.0	33.0	35.0	21.5	35.0
108-109	31.060570566401747	35.0	33.0	35.0	21.5	35.0
110-111	30.99498617566472	35.0	33.0	35.0	20.0	35.0
112-113	30.95666669336316	35.0	33.0	35.0	19.5	35.0
114-115	30.83104900319735	35.0	33.0	35.0	19.0	35.0
116-117	31.020302907337353	35.0	33.0	35.0	20.0	35.0
118-119	31.007135528319196	35.0	33.0	35.0	19.0	35.0
120-121	30.932861650751107	35.0	33.0	35.0	17.5	35.0
122-123	30.8012855252491	35.0	33.0	35.0	4.5	35.0
124-125	30.16231669518742	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40359.0
3	10795.0
4	5641.0
5	4533.0
6	4654.0
7	5048.0
8	5518.0
9	6019.0
10	6201.0
11	6810.0
12	7511.0
13	9451.0
14	12357.0
15	10995.0
16	6582.0
17	5594.0
18	5033.0
19	4753.0
20	4461.0
21	4686.0
22	5098.0
23	6512.0
24	8439.0
25	11058.0
26	13166.0
27	15559.0
28	18830.0
29	22870.0
30	27464.0
31	35254.0
32	45505.0
33	61898.0
34	96354.0
35	172741.0
36	404519.0
37	840365.0
38	294507.0
39	346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07282735773359	18.187992072982677	11.765247781596697	26.973932787687044
2	15.673538473038706	19.71141438896809	37.424825713121216	27.190221424871986
3	18.38819498008922	23.620009676099897	28.499229784228312	29.492565559582566
4	13.382692368542907	15.327892341268127	34.10350423004228	37.18591106014669
5	14.912070281468559	37.278106245535646	32.44785744326236	15.36196602973344
6	34.73548524982134	33.959984950387486	16.376115641578785	14.92841415821239
7	30.510462784273663	31.53147738832166	19.28175061601437	18.67630921139031
8	28.2425897271994	34.50141466011656	18.33947925550952	18.916516357174526
9	27.99020796937765	15.337079526866884	18.337046144944306	38.33566635881116
10-11	26.930499459376673	25.042224718986063	26.004972799645238	22.022303021992027
12-13	26.84724463994704	22.426910797081174	26.703044039504466	24.02280052346732
14-15	24.354406101696274	23.654001249705324	24.049459303214707	27.94213334538369
16-17	24.416889698510808	26.7931359612806	24.66128986304687	24.128684477161716
18-19	24.52103485181541	25.851287733840817	26.454699806221775	23.172977608121993
20-21	25.350564410035304	24.775517724486726	26.271190966591128	23.602726898886843
22-23	25.13797435602512	24.350162863913134	26.137872684270047	24.3739900957917
24-25	24.642746617798117	24.819129038379863	26.115789007646427	24.422335336175593
26-27	24.33493109313245	24.96027106667987	26.36737499819237	24.337422841995313
28-29	24.41462069984324	25.23608507472449	26.189168853278183	24.160125372154088
30-31	24.808582379434043	24.944959647456976	26.0908179165469	24.155640056562085
32-33	24.502094634447406	24.901598125208167	26.117729825196083	24.478577415148337
34-35	24.617456759899138	25.049735404533163	26.23299529721771	24.09981253834999
36-37	24.6449028919929	24.97883055679529	26.155864948074235	24.220401603137574
38-39	24.6349740571841	25.098078127009227	26.137058870903513	24.129888944903165
40-41	24.657607999202643	24.988230970213007	26.099369767342523	24.254791263241827
42-43	24.566769125075197	24.861663350265374	25.975676065312342	24.59589145934709
44-45	24.496772258689184	24.954703051359086	26.015465926727515	24.533058763224215
46-47	24.586320499494523	24.96769588927966	25.842532143925045	24.603451467300765
48-49	24.506139193617642	25.246891470091594	25.71206414395013	24.534905192340638
50-51	24.56509188716583	25.33629668191723	25.62226426973591	24.47634716118102
52-53	24.750024527574443	25.214657989949146	25.50534033705893	24.52997714541748
54-55	24.496780919657148	24.992958470326126	25.731284548547844	24.77897606146888
56-57	24.57240884655395	25.14438431768287	25.503009156056976	24.780197679706195
58-59	24.478965631008823	25.212566182560753	25.708455683518828	24.6000125029116
60-61	24.7301874182976	25.0966190668151	25.7445652609182	24.4286282539691
62-63	24.561072531877	25.183484340370736	25.76800905196828	24.48743407578398
64-65	24.4343902475922	24.912101788398235	25.7763118435443	24.877196120465268
66-67	24.420657929601717	25.26443352169186	25.622558705098363	24.692349843608067
68-69	24.454704360251633	25.22790591301744	25.605908145301825	24.7114815814291
70-71	24.381130738967897	25.069010440999413	25.815800409880197	24.7340584101525
72-73	24.52793501226618	25.308200732433512	25.475746955591017	24.68811729970929
74-75	24.389558548338403	25.369882433662998	25.54393035947137	24.696628658527224
76-77	24.40887038697763	25.01651311894718	25.596524603045534	24.978091891029656
78-79	24.244866704279097	25.120823996967275	25.66870331446186	24.96560598429177
80-81	24.378394348173916	25.419980369176937	25.351481611008925	24.850143671640225
82-83	24.427851664372866	25.337540041372936	25.473388144238378	24.76122015001582
84-85	24.298861867189213	25.340507676912488	25.336458648968406	25.02417180692989
86-87	24.214744830446108	25.493885167694035	25.399957997513667	24.89141200434619
88-89	24.16703967935444	25.27735680984718	25.40002829853711	25.155575212261276
90-91	24.177616174236384	25.15422303824891	25.53611819127728	25.132042596237426
92-93	24.255790692355813	25.338734034383307	25.526610621823675	24.878864651437205
94-95	24.186475425792377	25.11636081697852	25.55813257602303	25.139031181206068
96-97	24.082008133626402	25.194419477988482	25.52775132816602	25.195821060219092
98-99	23.996407525597604	25.23704368811947	25.36472024617838	25.401828540104542
100-101	23.94577763776949	25.24972346880025	25.534107887657587	25.270391005772673
102-103	23.904213709556316	25.170575780719712	25.602885744650134	25.32232476507384
104-105	23.97289682783341	25.225385163689563	25.506232296886388	25.295485711590636
106-107	23.950323896241077	25.186820433027407	25.499459616752816	25.363396053978704
108-109	23.942440236393615	25.1431852625078	25.50521972130043	25.409154779798154
110-111	23.798706419869156	25.467506213060076	25.411932946337533	25.32185442073323
112-113	23.718626257268973	25.499843046469145	25.40415812284022	25.37737257342166
114-115	23.71979180293003	25.554486212594874	25.362738633299607	25.36298335117549
116-117	23.554768590111955	25.402394667916084	25.491540084402263	25.551296657569694
118-119	23.611989269583255	25.260929865629517	25.461646858229553	25.665434006557675
120-121	23.486782519433937	25.443647308704666	25.507231287054964	25.562338884806437
122-123	23.404655769522122	25.589335027683358	25.325749693154904	25.68025950963962
124-125	23.298375273131885	25.552516488134756	25.350202347547814	25.798905891185548
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	20.0
2	25.5
3	56.0
4	63.5
5	62.5
6	162.0
7	502.5
8	862.0
9	959.0
10	817.0
11	629.0
12	611.0
13	684.5
14	736.5
15	815.5
16	904.5
17	943.5
18	1020.5
19	1112.0
20	1197.0
21	1307.0
22	1424.0
23	1672.0
24	1924.5
25	2230.0
26	2858.5
27	3834.5
28	5047.0
29	6769.0
30	9112.0
31	11646.5
32	15160.0
33	18815.5
34	22229.5
35	26064.0
36	30684.5
37	35199.0
38	39173.5
39	44281.0
40	49232.5
41	53268.0
42	57820.5
43	64254.5
44	71196.0
45	77630.0
46	82291.0
47	86712.5
48	93611.5
49	99378.5
50	101107.0
51	101351.5
52	101371.5
53	101002.5
54	100847.5
55	98426.5
56	92588.0
57	85202.0
58	78177.5
59	70439.0
60	60513.0
61	51456.0
62	42689.0
63	32545.5
64	24166.0
65	18420.0
66	14276.0
67	11076.5
68	8511.0
69	6579.0
70	5146.0
71	3901.0
72	2909.5
73	2266.0
74	1814.5
75	1387.0
76	961.5
77	545.5
78	311.5
79	178.0
80	104.0
81	73.5
82	38.5
83	21.5
84	14.0
85	11.0
86	9.5
87	8.0
88	5.5
89	3.0
90	2.5
91	1.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02166865555558522
2	0.0018687546885720312
3	0.03172433554647282
4	0.1626261520650184
5	0.02318145697014353
6	0.07034526577696146
7	0.052992543668792594
8	0.04493910084423217
9	0.033860055190555134
10-11	0.0652951787018918
12-13	0.05826510154012083
14-15	0.06035632702495144
16-17	0.052058166324506584
18-19	0.03761981164732505
20-21	0.023937857677422684
22-23	0.002558414156973614
24-25	0.0042936863677905
26-27	0.0030478499087424793
28-29	0.0043159334474163575
30-31	0.0019577430070754614
32-33	0.008743102292962003
34-35	0.004137956810409497
36-37	0.006362664772995248
38-39	0.0019132488478237463
40-41	0.002758637873606332
42-43	0.003114591147620052
44-45	0.004182450969661212
46-47	0.004182450969661212
48-49	0.0018242605293203162
50-51	0.0012680835386738782
52-53	6.89659468401583E-4
54-55	0.004671886721430078
56-57	5.339299110205803E-4
58-59	4.2269451289129274E-4
60-61	0.0
62-63	8.453890257825855E-4
64-65	0.0
66-67	0.003893238934525065
68-69	5.561769906464378E-4
70-71	0.0
72-73	0.0020689784052047486
74-75	0.0051835695528248005
76-77	0.0013570718571773084
78-79	8.898831850343005E-5
80-81	0.0
82-83	0.0054727815879609485
84-85	0.0014683072553065958
86-87	0.0
88-89	8.898831850343004E-4
90-91	4.671886721430078E-4
92-93	0.0
94-95	0.002602908316225329
96-97	0.0011568481405445908
98-99	4.449415925171502E-4
100-101	0.0
102-103	0.0010901069016670182
104-105	0.0
106-107	3.1145911476200517E-4
108-109	0.0021357196440823212
110-111	1.1123539812928756E-4
112-113	2.4471787588443267E-4
114-115	0.0
116-117	0.0015350484941841684
118-119	0.0019132488478237463
120-121	0.002869873271735619
122-123	7.341536276532979E-4
124-125	0.10956686715734826
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2247486.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.64674943136233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07821634814775	42.89152191741713
2	12.130080596367238	13.499991110565556
3	4.09061378174664	6.828880803999983
4	1.969411530751574	4.383653999158742
5	1.1632728411356348	3.2366176155491817
6	0.7274901272063755	2.428947649346585
7	0.5073544309665389	1.9762837425020483
8	0.3625586817994583	1.6140169696207587
9	0.28484477126211555	1.4265617051930994
>10	1.5733463217175312	15.536676234536975
>50	0.08432931097932025	3.1662141388557696
>100	0.02768002285975608	2.478632450448369
>500	5.608645419816551E-4	0.19310251438247472
>1k	2.4037051799201512E-4	0.33889914842312496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3166	0.14086850819092978	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2654	0.11808749865405167	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.449415925171503E-5
7	0.0	0.0	0.0	0.0	4.449415925171503E-5
8	0.0	0.0	0.0	0.0	4.449415925171503E-5
9	0.0	0.0	0.0	0.0	4.449415925171503E-5
10-11	0.0	0.0	0.0	0.0	6.674123887757255E-5
12-13	0.0	0.0	0.0	0.0	2.2247079625857514E-4
14-15	0.0	0.0	0.0	0.0	2.892120351361477E-4
16-17	0.0	0.0	0.0	2.2247079625857515E-5	4.0044743326543526E-4
18-19	0.0	0.0	0.0	4.449415925171503E-5	4.0044743326543526E-4
20-21	0.0	0.0	0.0	4.449415925171503E-5	4.0044743326543526E-4
22-23	0.0	0.0	0.0	1.5572955738100259E-4	4.2269451289129274E-4
24-25	0.0	0.0	0.0	6.89659468401583E-4	4.894357517688653E-4
26-27	0.0	0.0	0.0	0.0010678598220411608	6.451653091498679E-4
28-29	0.0	0.0	0.0	0.002936614510613192	6.674123887757254E-4
30-31	4.449415925171503E-5	0.0	0.0	0.011168033972180472	6.674123887757254E-4
32-33	4.449415925171503E-5	0.0	0.0	0.025806612365994717	7.119065480274405E-4
34-35	4.449415925171503E-5	0.0	0.0	0.045428536596001046	8.453890257825855E-4
36-37	4.449415925171503E-5	0.0	0.0	0.07517288205577254	8.453890257825855E-4
38-39	4.449415925171503E-5	0.0	0.0	0.11982277086486856	8.67636105408443E-4
40-41	4.449415925171503E-5	0.0	0.0	0.1710133010839667	8.898831850343005E-4
42-43	4.449415925171503E-5	0.0	0.0	0.2193562051109551	9.121302646601581E-4
44-45	4.449415925171503E-5	0.0	0.0	0.27606401107726586	9.788715035377307E-4
46-47	4.449415925171503E-5	0.0	0.0	0.3389120110203134	0.0010233656627894455
48-49	4.449415925171503E-5	0.0	0.0	0.40489684919060676	0.001045612742415303
50-51	4.449415925171503E-5	0.0	0.0	0.47446346718066323	0.0011568481405445908
52-53	4.449415925171503E-5	0.0	0.0	0.5468332172035777	0.0011790952201704483
54-55	4.449415925171503E-5	0.0	0.0	0.6226512645685001	0.0012013422997963058
56-57	4.449415925171503E-5	0.0	0.0	0.7067674726338673	0.0012013422997963058
58-59	4.449415925171503E-5	0.0	0.0	0.7985144290109037	0.0012235893794221631
60-61	4.449415925171503E-5	0.0	0.0	0.8909287977767159	0.0012458364590480207
62-63	4.449415925171503E-5	0.0	0.0	0.9886379714934821	0.001379318936803166
64-65	4.449415925171503E-5	0.0	0.0	1.0913972322853178	0.001379318936803166
66-67	4.449415925171503E-5	0.0	0.0	1.2095069780189953	0.001423813096054881
68-69	4.449415925171503E-5	0.0	0.0	1.3388292518841052	0.001423813096054881
70-71	4.449415925171503E-5	0.0	0.0	1.4711326344190798	0.0014683072553065958
72-73	6.674123887757255E-5	0.0	0.0	1.6059054427925246	0.0017130251311910285
74-75	8.898831850343006E-5	0.0	0.0	1.7486204585924006	0.001735272210816886
76-77	8.898831850343006E-5	0.0	0.0	1.9147394021586788	0.001779766370068601
78-79	8.898831850343006E-5	0.0	0.0	2.092315591732273	0.0018242605293203162
80-81	8.898831850343006E-5	0.0	0.0	2.2742299618329103	0.0018687546885720312
82-83	8.898831850343006E-5	0.0	0.0	2.4664224827206933	0.0019577430070754614
84-85	8.898831850343006E-5	0.0	0.0	2.6688931543956222	0.001979990086701319
86-87	8.898831850343006E-5	0.0	0.0	2.8888945248157274	0.002046731325578891
88-89	8.898831850343006E-5	0.0	0.0	3.1406647249415567	0.0021357196440823212
90-91	8.898831850343006E-5	0.0	0.0	3.4038921710747028	0.0021579667237081788
92-93	8.898831850343006E-5	0.0	0.0	3.6722586926014222	0.0023136962810891815
94-95	8.898831850343006E-5	4.449415925171503E-5	0.0	3.9490568573063416	0.0023581904403408966
96-97	8.898831850343006E-5	4.449415925171503E-5	0.0	4.255821838267291	0.0023581904403408966
98-99	1.3348247775514508E-4	4.449415925171503E-5	0.0	4.59749248716121	0.0023581904403408966
100-101	1.3348247775514508E-4	4.449415925171503E-5	0.0	4.972511508414291	0.0023581904403408966
102-103	1.3348247775514508E-4	4.449415925171503E-5	0.0	5.3521134280702976	0.0024026845995926117
104-105	1.3348247775514508E-4	4.449415925171503E-5	0.0	5.740080249665626	0.0024026845995926117
106-107	1.3348247775514508E-4	4.449415925171503E-5	0.0	6.160572301673959	0.0024249316792184688
108-109	1.3348247775514508E-4	4.449415925171503E-5	0.0	6.624557394350844	0.0024916729180960414
110-111	1.3348247775514508E-4	4.449415925171503E-5	0.0	7.103603759934433	0.0025806612365994715
112-113	1.3348247775514508E-4	4.449415925171503E-5	0.0	7.596332079487926	0.0025806612365994715
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3095	0.0	58.76982	1
GTATCAA	5570	0.0	55.568336	1
TCAACGC	7620	0.0	39.982037	4
ATCAACG	7875	0.0	38.765682	3
CAACGCA	7885	0.0	38.60818	5
TATCAAC	7950	0.0	38.400826	2
AACGCAG	8180	0.0	37.22413	6
GTACATG	12025	0.0	33.971966	1
TACATGG	12480	0.0	32.29757	2
ACGCAGA	9685	0.0	31.437586	7
ACATGGG	12510	0.0	31.409147	3
CATGGGG	10815	0.0	30.320415	4
CGCAGAG	10420	0.0	29.158976	8
ATGGGGG	6420	0.0	28.766485	5
GCAGAGT	11395	0.0	26.716307	9
AGAGTAC	10540	0.0	24.842089	10-11
GAGTACT	4835	0.0	22.037321	12-13
CAGAGTA	11150	0.0	21.773216	10-11
ATGGGGA	4175	0.0	20.97592	5
GTGGTAT	1835	0.0	20.799799	1
>>END_MODULE