FastQCFastQC Report
Mon 15 Aug 2016
SRR2921626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524786
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTCG10300.038.1371542
GTATCAA41350.036.5642621
GTACATG92900.033.1904141
CATGGGG74500.032.9141434
TACATGG94800.032.1442452
ACATGGG95850.031.3574583
TCAACGC47650.031.3510744
CAACGCA48800.030.7322085
ATGGGGG48750.030.2754145
TATCAAC49850.029.8480822
GGTATCA28700.029.6587451
AACGCAG52300.029.0131266
ATCAACG52050.028.7008363
CCTCGGT9500.026.9393064
TACCGTA1901.1710654E-625.0466637
ACGCAGA60400.025.0155627
GCCTCGG13150.024.8930723
TATCACG1250.001631044123.806832
CGCAGAG63500.023.7006518
TCGGTCT6450.023.064096
Produced by FastQC (version 0.11.5)