##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921626_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1524786
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.400533583073297	31.0	30.0	34.0	25.0	34.0
2	29.365770672081197	31.0	30.0	34.0	23.0	34.0
3	29.474561676195872	31.0	31.0	34.0	23.0	34.0
4	32.98451323661156	37.0	35.0	37.0	26.0	37.0
5	32.92514490558019	37.0	35.0	37.0	26.0	37.0
6	33.023755464701274	37.0	35.0	37.0	26.0	37.0
7	32.808440659869646	37.0	35.0	37.0	25.0	37.0
8	32.82187139703539	37.0	35.0	37.0	25.0	37.0
9	34.44394623245491	39.0	35.0	39.0	23.0	39.0
10-11	34.50458720108921	39.0	37.0	39.0	25.0	39.0
12-13	34.44247455052709	39.0	37.0	39.0	23.5	39.0
14-15	35.65543197537228	40.0	37.0	41.0	23.0	41.0
16-17	35.59997927578034	40.0	37.0	41.0	22.5	41.0
18-19	35.53318104966861	40.0	37.0	41.0	22.0	41.0
20-21	35.62047985750131	40.0	37.0	41.0	22.5	41.0
22-23	35.54359333047391	40.0	37.0	41.0	22.5	41.0
24-25	35.54365629012858	40.0	37.0	41.0	23.0	41.0
26-27	35.52401845242545	40.0	37.0	41.0	23.5	41.0
28-29	35.464626183608715	40.0	36.0	41.0	23.0	41.0
30-31	35.35339713245006	40.0	36.0	41.0	23.0	41.0
32-33	35.26457581588498	40.0	36.0	41.0	21.5	41.0
34-35	35.126532510135846	40.0	36.0	41.0	20.0	41.0
36-37	35.00918096047577	40.0	35.0	41.0	18.0	41.0
38-39	34.8831331085149	40.0	35.0	41.0	16.5	41.0
40-41	34.765951746671334	40.0	35.0	41.0	15.5	41.0
42-43	34.470997897409866	40.0	35.0	41.0	12.0	41.0
44-45	34.04611466789438	39.0	34.5	41.0	9.0	41.0
46-47	33.75396383492503	39.0	34.0	40.5	7.5	41.0
48-49	33.53067807548076	38.0	33.5	40.0	4.0	41.0
50-51	33.31707400251576	38.0	33.0	40.0	2.0	41.0
52-53	33.11059584754844	38.0	33.0	40.0	2.0	41.0
54-55	32.869398722181344	38.0	33.0	40.0	2.0	41.0
56-57	32.61363201131175	37.5	32.5	40.0	2.0	41.0
58-59	32.618353657496854	37.0	33.0	40.0	2.0	41.0
60-61	32.40882589425664	37.0	32.0	40.0	2.0	41.0
62-63	32.09860072167504	36.0	32.0	39.5	2.0	41.0
64-65	31.804604383828288	36.0	32.0	39.0	2.0	41.0
66-67	31.4866899355057	35.0	31.5	39.0	2.0	40.5
68-69	31.156481302950056	35.0	31.0	38.5	2.0	40.0
70-71	30.79963942481109	35.0	31.0	37.5	2.0	40.0
72-73	30.456403062462535	35.0	31.0	37.0	2.0	39.0
74-75	30.09780946309843	35.0	30.5	36.5	2.0	39.0
76-77	29.809139118538603	35.0	30.0	36.0	2.0	38.5
78-79	29.540466662207024	35.0	30.0	35.5	2.0	37.0
80-81	29.292704025351753	34.5	30.0	35.0	2.0	37.0
82-83	29.07687800124083	34.0	30.0	35.0	2.0	36.0
84-85	28.871316696244588	34.0	29.5	35.0	2.0	36.0
86-87	28.700036595299274	34.0	29.0	35.0	2.0	36.0
88-89	28.541556323313564	34.0	29.0	35.0	2.0	35.0
90-91	28.361156581972814	34.0	29.0	35.0	2.0	35.0
92-93	28.281699202379876	34.0	29.0	35.0	2.0	35.0
94-95	28.156220282715083	34.0	29.0	35.0	2.0	35.0
96-97	28.065407867071183	34.0	29.0	35.0	2.0	35.0
98-99	27.97330871348504	34.0	29.0	35.0	2.0	35.0
100-101	27.858425051121927	34.0	28.5	35.0	2.0	35.0
102-103	26.958910955373412	32.5	26.0	34.0	2.0	35.0
104-105	27.882542205922668	34.0	29.0	35.0	2.0	35.0
106-107	28.170730187711584	34.0	29.0	35.0	2.0	35.0
108-109	28.166933589369265	34.0	29.0	35.0	2.0	35.0
110-111	28.106020779309357	34.0	29.0	35.0	2.0	35.0
112-113	28.01887412397543	34.0	29.0	35.0	2.0	35.0
114-115	27.905394593077325	34.0	29.0	35.0	2.0	35.0
116-117	27.78116273365574	34.0	29.0	35.0	2.0	35.0
118-119	27.492575679472395	34.0	29.0	35.0	2.0	35.0
120-121	27.35552169288018	34.0	27.5	35.0	2.0	35.0
122-123	27.110780463619157	34.0	27.0	35.0	2.0	35.0
124-125	26.33236532864284	33.5	25.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88251.0
3	13863.0
4	6844.0
5	5741.0
6	6017.0
7	6384.0
8	6455.0
9	6796.0
10	7314.0
11	7557.0
12	7609.0
13	7992.0
14	8374.0
15	7449.0
16	6063.0
17	5356.0
18	4898.0
19	4549.0
20	4503.0
21	4710.0
22	5445.0
23	6950.0
24	10238.0
25	13439.0
26	14572.0
27	16281.0
28	19409.0
29	23130.0
30	27979.0
31	34994.0
32	45816.0
33	63237.0
34	97592.0
35	155799.0
36	291817.0
37	391756.0
38	89569.0
39	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92206487891373	17.731527431286487	12.288959196646045	25.05744849315374
2	16.471863005144915	20.209207326191844	38.43712367474479	24.88180599391845
3	20.678975299435074	23.946410288196212	27.8002205138195	27.574393898549214
4	13.69235411023147	15.203625845298141	34.040639901351824	37.06338014311857
5	14.686547231099425	37.65828098843952	31.892996017065222	15.762175763395833
6	34.096955859869645	33.60462085938059	17.480124436030255	14.81829884471951
7	29.72118466331906	31.788806963296228	19.08390183978224	19.406106533602472
8	28.672384039474807	34.465456263410346	18.43301553139415	18.429144165720697
9	28.368203911041483	16.12010619601391	17.96049597500838	37.55119391793622
10-11	26.7299410694378	25.602576645492086	25.901592982138418	21.765889302931697
12-13	26.376090750150123	23.084072579917834	26.34060560225872	24.199231067673324
14-15	24.45269061730509	24.22711810034943	23.61200083839371	27.708190443951775
16-17	25.02836658650652	27.120096861354888	24.08681569607946	23.76472085605913
18-19	24.817020422262832	26.445834731657836	25.491168658110396	23.24597618796893
20-21	25.511868258460506	25.301872706351297	25.452300635431712	23.73395839975648
22-23	25.786124528108772	24.73700716316775	25.146668451225768	24.330199857497707
24-25	25.161785944951614	25.20800347172678	25.11087778203106	24.519332801290545
26-27	24.609003491599722	25.43372702271164	25.43769661367645	24.519572872012183
28-29	24.885718766136666	25.661459847213848	25.34815834487573	24.10466304177375
30-31	25.487858918039702	25.2809565527993	25.289451751372106	23.941732777788893
32-33	24.814360941765194	25.52818322028906	25.313629800390856	24.343826037554887
34-35	24.812337668515543	25.83305146939417	25.36900117808738	23.985609684002913
36-37	25.141253776687687	25.64622229568173	25.155289789554914	24.05723413807567
38-39	25.03311001972498	25.76651581573315	25.14078728050468	24.0595868840372
40-41	24.893717462813825	25.501411796894146	25.307496810457696	24.297373929834336
42-43	24.95210519048211	25.444451696678343	25.084350951123184	24.51909216171636
44-45	24.682882128838603	25.4013743414286	25.517936231397325	24.397807298335465
46-47	24.56840877528918	25.42568732833601	25.448876521319296	24.55702737505551
48-49	24.610016804120637	25.744155541276044	25.28736423940115	24.35846341520217
50-51	24.58628376348276	25.694917322354254	25.384982753349945	24.33381616081304
52-53	24.690433168889196	25.643216457839774	25.211710486913617	24.454639886357413
54-55	24.483505194877182	25.341265892368614	25.46596787202074	24.709261040733466
56-57	24.639898714082122	25.51814733361869	25.1077884869268	24.734165465372385
58-59	24.639997900958015	25.622874310021942	25.307198074128976	24.429929714891063
60-61	24.97237492952286	25.548360518895418	25.43644987423172	24.042814677349998
62-63	24.712222570619282	25.633871278802346	25.370299427593835	24.28360672298454
64-65	24.554148765654197	25.39634227337146	25.377909655903704	24.671599305070643
66-67	24.68367945047228	25.535116788088928	25.20120692997438	24.579996831464413
68-69	24.505439071444922	25.490526641708755	25.40462585105413	24.599408435792192
70-71	24.485296650552883	25.454879491095316	25.491098597001756	24.568725261350046
72-73	24.62251350233542	25.829991436303445	25.151029037523813	24.396466023837316
74-75	24.483672009688867	25.702257554505614	25.273401233671105	24.54066920213441
76-77	24.483747948569608	25.3319020875723	25.326326657247467	24.858023306610626
78-79	24.436230793603105	25.472161015253604	25.502697078968033	24.588911112175264
80-81	24.60327924339684	25.71259522806852	25.155887380632834	24.528238147901803
82-83	24.63185045799551	25.606290152711093	25.326018612391838	24.435840776901557
84-85	24.617777169968676	25.776670165941724	25.063631381708596	24.541921282381
86-87	24.393495636317127	25.858814114652855	25.22339891703263	24.52429133199739
88-89	24.27343048915525	25.503600048280532	25.410548508813818	24.8124209537504
90-91	24.656083024836015	25.34150628468647	25.381422071232464	24.62098861924505
92-93	24.622599279517406	25.54988038414807	25.362200734911923	24.4653196014226
94-95	24.652823440324987	25.455514995370983	25.252284092992323	24.639377471311715
96-97	24.423800965524478	25.36899170908328	25.390834076717216	24.816373248675028
98-99	24.318370082856134	25.42679139576478	25.307849262571892	24.946989258807196
100-101	24.247167583121232	25.664991173242214	25.34396151710699	24.743879726529563
102-103	24.072683413763297	25.54511576536575	25.554264974205132	24.82793584666582
104-105	24.21726023291421	25.58130993500793	25.39429254268558	24.80713728939228
106-107	24.154496541122214	25.501976688973826	25.549328030682624	24.79419873922134
108-109	24.156117001825756	25.536305375696628	25.515643873093772	24.791933749383844
110-111	23.93371953542944	25.96272338717558	25.481501059508922	24.622056017886056
112-113	23.888757901723338	25.985532677071237	25.49453302565377	24.631176395551655
114-115	23.90152074171004	26.122190628925246	25.376381113548934	24.59990751581578
116-117	23.881084998548896	25.933550971419017	25.397675940060438	24.78768808997165
118-119	23.834527597751183	25.75540197621687	25.536991505930658	24.873078920101293
120-121	23.83233913869548	25.89265190092791	25.692260825167885	24.58274813520872
122-123	23.781375981867463	25.956650596515026	25.529865757066787	24.732107664550725
124-125	23.67296831347642	25.888626937048336	25.530666292982335	24.907738456492915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	514.0
1	423.0
2	297.0
3	238.5
4	198.0
5	178.0
6	199.0
7	249.0
8	309.0
9	340.0
10	333.5
11	354.0
12	379.0
13	395.0
14	454.0
15	497.0
16	537.5
17	591.5
18	660.0
19	746.5
20	813.5
21	934.0
22	1077.0
23	1240.0
24	1493.0
25	1863.0
26	2485.0
27	3305.0
28	4199.5
29	5328.0
30	6908.5
31	8648.0
32	10731.0
33	13061.0
34	15269.5
35	17712.5
36	20627.0
37	23711.0
38	26296.5
39	29128.5
40	32236.0
41	34937.5
42	38051.0
43	41937.5
44	46265.0
45	50617.0
46	54631.5
47	58723.0
48	63437.0
49	67569.0
50	70472.5
51	71464.5
52	70108.5
53	68627.5
54	67955.0
55	66459.5
56	62983.5
57	57767.0
58	52267.5
59	46308.5
60	39996.0
61	34482.5
62	28959.0
63	22586.5
64	16845.0
65	12873.5
66	9756.5
67	7551.0
68	5933.5
69	4533.0
70	3577.5
71	2823.5
72	2148.0
73	1740.5
74	1381.0
75	1090.0
76	836.0
77	556.0
78	393.5
79	289.5
80	227.5
81	168.0
82	112.5
83	94.0
84	77.5
85	68.0
86	66.0
87	57.5
88	43.0
89	33.5
90	29.5
91	30.0
92	31.5
93	25.0
94	18.5
95	17.0
96	17.0
97	11.5
98	7.5
99	5.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0247247810512426
2	0.011411437408265815
3	0.01082119064576931
4	0.011542603355487261
5	0.018494398558223907
6	0.09398040118416617
7	0.059680505985758
8	0.050892387521921115
9	0.02852859352066454
10-11	0.051744966178860506
12-13	0.013411718103392868
14-15	0.02902046582274496
16-17	0.03577551210464944
18-19	0.04200589459766813
20-21	0.030692831649818397
22-23	0.039349784166433845
24-25	0.030987955031066654
26-27	0.045875290040700796
28-29	0.04558016665945254
30-31	0.025938066063040978
32-33	0.029971418940100445
34-35	0.04626878788236513
36-37	0.0089520758978637
38-39	0.021183300476263555
40-41	0.043579885964325486
42-43	0.023052415224169162
44-45	0.017969734769338122
46-47	0.024200117262356817
48-49	0.03000421042690581
50-51	0.02833184459983237
52-53	0.0234459130658335
54-55	0.023052415224169162
56-57	0.025478985247765915
58-59	0.018428815584613185
60-61	0.023314747118612054
62-63	0.022560542922088738
64-65	0.020625845200572406
66-67	0.0277088063505305
68-69	0.023150789684585244
70-71	0.047777196275411764
72-73	0.021478423857511808
74-75	0.0098046545548031
76-77	0.015739913666573537
78-79	0.023544287526249586
80-81	0.01888789639988825
82-83	0.024364074696383624
84-85	0.012100058631178408
86-87	0.017772985848505955
88-89	0.023741036447081754
90-91	0.021412840883901087
92-93	0.00744366750481707
94-95	0.01085398213257467
96-97	0.014821752036023414
98-99	0.006230382493018692
100-101	0.010526067264521054
102-103	0.004197310311086277
104-105	0.00403335287705947
106-107	9.181616305501231E-4
108-109	0.01387079891866793
110-111	0.0015084083930466308
112-113	0.0025905274576235615
114-115	0.01318217769575534
116-117	0.005476178296495377
118-119	0.27902276122682135
120-121	0.001147702038187654
122-123	8.525786569393999E-4
124-125	0.313912903187726
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1524786.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.81019270045023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.5889862965309	55.01007295809992
2	8.880549615568098	11.688613629728914
3	2.8754979284138424	5.677112183359812
4	1.4148001362988187	3.72433078409794
5	0.7997287631012748	2.63151520038938
6	0.5123171695661144	2.0229414991737134
7	0.3614991673695929	1.6653230905951655
8	0.2599280120511544	1.3684730049065137
9	0.19860162824876054	1.1763010283106698
>10	1.0587335806205231	12.077911847970698
>50	0.04031369935143683	1.7555713685186911
>100	0.00844775256067107	0.9067535049982496
>500	4.968752657478634E-4	0.22739202388728028
>1k	9.937505314957268E-5	0.06768787596299541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.558297361072307E-5	0.0
2	0.0	0.0	0.0	6.558297361072307E-5	0.0
3	0.0	0.0	0.0	6.558297361072307E-5	0.0
4	0.0	0.0	0.0	6.558297361072307E-5	6.558297361072307E-5
5	0.0	0.0	0.0	6.558297361072307E-5	6.558297361072307E-5
6	0.0	0.0	0.0	6.558297361072307E-5	1.9674892083216923E-4
7	0.0	0.0	0.0	6.558297361072307E-5	1.9674892083216923E-4
8	0.0	0.0	0.0	6.558297361072307E-5	1.9674892083216923E-4
9	0.0	0.0	0.0	1.3116594722144614E-4	1.9674892083216923E-4
10-11	0.0	0.0	0.0	1.3116594722144614E-4	1.9674892083216923E-4
12-13	0.0	0.0	0.0	2.2954040763753075E-4	1.9674892083216923E-4
14-15	0.0	0.0	0.0	2.623318944428923E-4	1.9674892083216923E-4
16-17	0.0	0.0	0.0	3.279148680536154E-4	1.9674892083216923E-4
18-19	6.558297361072307E-5	0.0	0.0	3.607063548589769E-4	1.9674892083216923E-4
20-21	6.558297361072307E-5	0.0	0.0	3.9349784166433846E-4	1.9674892083216923E-4
22-23	6.558297361072307E-5	0.0	0.0	4.262893284697E-4	2.623318944428923E-4
24-25	6.558297361072307E-5	0.0	0.0	6.886212229125923E-4	3.279148680536154E-4
26-27	9.83744604160846E-5	0.0	0.0	0.0010165360909662076	3.279148680536154E-4
28-29	1.3116594722144614E-4	0.0	0.0	0.0022954040763753076	3.279148680536154E-4
30-31	1.3116594722144614E-4	0.0	0.0	0.010362109830494246	3.9349784166433846E-4
32-33	1.3116594722144614E-4	0.0	0.0	0.02767601486372514	4.5908081527506155E-4
34-35	1.3116594722144614E-4	0.0	0.0	0.04820348560388146	4.5908081527506155E-4
36-37	1.3116594722144614E-4	0.0	0.0	0.07942098104258564	5.246637888857846E-4
38-39	1.3116594722144614E-4	0.0	0.0	0.12910008355270838	5.246637888857846E-4
40-41	1.3116594722144614E-4	0.0	0.0	0.1866491428961179	5.246637888857846E-4
42-43	1.3116594722144614E-4	0.0	0.0	0.2394106451659446	5.574552756911461E-4
44-45	1.3116594722144614E-4	0.0	0.0	0.2982385724947632	6.230382493018692E-4
46-47	1.3116594722144614E-4	0.0	0.0	0.36503483111728463	6.558297361072308E-4
48-49	1.3116594722144614E-4	0.0	0.0	0.4343232427370136	6.558297361072308E-4
50-51	1.3116594722144614E-4	0.0	0.0	0.5048905223421516	7.869956833286769E-4
52-53	1.3116594722144614E-4	0.0	0.0	0.5815242270062815	9.83744604160846E-4
54-55	1.3116594722144614E-4	0.0	0.0	0.6671100075682752	0.0010821190645769307
56-57	1.3116594722144614E-4	0.0	0.0	0.7535483667872082	0.0011804935249930153
58-59	1.3116594722144614E-4	0.0	0.0	0.8498241720477497	0.0011804935249930153
60-61	1.3116594722144614E-4	0.0	0.0	0.9477067601617539	0.0011804935249930153
62-63	1.3116594722144614E-4	0.0	0.0	1.0572631175784668	0.0012460764986037384
64-65	1.3116594722144614E-4	0.0	0.0	1.1606546754757718	0.0012788679854091
66-67	1.3116594722144614E-4	0.0	0.0	1.2799828959604824	0.0013116594722144615
68-69	1.3116594722144614E-4	0.0	0.0	1.4175103916221685	0.0013116594722144615
70-71	1.3116594722144614E-4	0.0	0.0	1.5546771809289959	0.0013772424458251846
72-73	1.3116594722144614E-4	0.0	0.0	1.6867940812677977	0.0016067828534627154
74-75	1.3116594722144614E-4	0.0	0.0	1.8262890661378055	0.001639574340268077
76-77	1.3116594722144614E-4	0.0	0.0	1.9939847296604245	0.001639574340268077
78-79	1.3116594722144614E-4	0.0	0.0	2.1774530983364224	0.001639574340268077
80-81	1.3116594722144614E-4	0.0	0.0	2.359904930921454	0.001639574340268077
82-83	1.3116594722144614E-4	0.0	0.0	2.54776735882937	0.001639574340268077
84-85	1.3116594722144614E-4	0.0	0.0	2.743302994649741	0.001639574340268077
86-87	1.3116594722144614E-4	0.0	0.0	2.957792109843611	0.001639574340268077
88-89	1.3116594722144614E-4	0.0	0.0	3.210581681626143	0.001639574340268077
90-91	1.3116594722144614E-4	0.0	0.0	3.4704542145586332	0.001639574340268077
92-93	1.3116594722144614E-4	0.0	0.0	3.7270803902973926	0.001770740287489523
94-95	1.3116594722144614E-4	0.0	0.0	3.9870185062034933	0.0019019062347109693
96-97	1.3116594722144614E-4	0.0	0.0	4.284961955317009	0.0019346977215163306
98-99	1.3116594722144614E-4	0.0	0.0	4.618156252746287	0.001967489208321692
100-101	1.3116594722144614E-4	0.0	0.0	4.9773869906990225	0.001967489208321692
102-103	1.3116594722144614E-4	0.0	0.0	5.349242451071822	0.0020658636687377768
104-105	1.3116594722144614E-4	0.0	0.0	5.73044348518415	0.0021642381291538614
106-107	1.3116594722144614E-4	0.0	0.0	6.13679558967619	0.0021642381291538614
108-109	1.3116594722144614E-4	0.0	0.0	6.564363786131299	0.002197029615959223
110-111	1.3116594722144614E-4	0.0	0.0	7.042135748885418	0.0022954040763753076
112-113	1.3116594722144614E-4	0.0	0.0	7.516956477827052	0.0022954040763753076
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCTCG	1030	0.0	38.137154	2
GTATCAA	4135	0.0	36.564262	1
GTACATG	9290	0.0	33.190414	1
CATGGGG	7450	0.0	32.914143	4
TACATGG	9480	0.0	32.144245	2
ACATGGG	9585	0.0	31.357458	3
TCAACGC	4765	0.0	31.351074	4
CAACGCA	4880	0.0	30.732208	5
ATGGGGG	4875	0.0	30.275414	5
TATCAAC	4985	0.0	29.848082	2
GGTATCA	2870	0.0	29.658745	1
AACGCAG	5230	0.0	29.013126	6
ATCAACG	5205	0.0	28.700836	3
CCTCGGT	950	0.0	26.939306	4
TACCGTA	190	1.1710654E-6	25.046663	7
ACGCAGA	6040	0.0	25.015562	7
GCCTCGG	1315	0.0	24.893072	3
TATCACG	125	0.0016310441	23.80683	2
CGCAGAG	6350	0.0	23.700651	8
TCGGTCT	645	0.0	23.06409	6
>>END_MODULE