FastQCFastQC Report
Mon 15 Aug 2016
SRR2921626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524786
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.19930665680298743No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.1631048553698683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.11431112300349033No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34350.043.4215241
GTATCAA51950.040.4248961
TCAACGC64550.032.430964
ATGGGGG43100.032.3510135
CATGGGG72900.032.3160674
GTACATG92100.032.0654531
CAACGCA65950.031.7133125
TATCAAC66300.031.3091872
ATCAACG67950.030.8122733
TACATGG95500.030.6679122
AACGCAG68100.030.631636
ACATGGG96750.029.778463
ACGCAGA81650.025.6195557
TGGGGGG30950.025.4192336
TATCACG1651.0887574E-525.3058092
CGCAGAG85400.024.3534038
GTATAGG4950.022.9002481
GAGTACT38150.022.86388812-13
GCAGAGT92350.022.4561069
GTACTTT40300.021.93202614-15
Produced by FastQC (version 0.11.5)