##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921626_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1524786 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.523432796471113 33.0 31.0 34.0 30.0 34.0 2 31.706531277175944 33.0 31.0 34.0 30.0 34.0 3 31.78968392941698 34.0 31.0 34.0 30.0 34.0 4 35.17720519469617 37.0 35.0 37.0 33.0 37.0 5 34.99807448389479 37.0 35.0 37.0 33.0 37.0 6 35.01484732939573 37.0 35.0 37.0 33.0 37.0 7 34.900142708550575 37.0 35.0 37.0 32.0 37.0 8 34.93569917352337 37.0 35.0 37.0 32.0 37.0 9 36.61791949821155 39.0 38.0 39.0 33.0 39.0 10-11 36.57332110866705 39.0 38.0 39.0 33.0 39.0 12-13 36.492788168306895 39.0 38.0 39.0 33.0 39.0 14-15 37.890788936939344 41.0 38.5 41.0 33.0 41.0 16-17 37.8926688728779 41.0 38.5 41.0 33.0 41.0 18-19 37.91173712245522 41.0 39.0 41.0 33.0 41.0 20-21 37.85919794646593 41.0 39.0 41.0 33.0 41.0 22-23 37.86214852444867 41.0 39.0 41.0 33.0 41.0 24-25 37.818832609953134 41.0 39.0 41.0 33.0 41.0 26-27 37.74275373724575 41.0 38.0 41.0 33.0 41.0 28-29 37.66487198859381 41.0 38.0 41.0 32.5 41.0 30-31 37.54127038154863 41.0 38.0 41.0 32.0 41.0 32-33 37.40898755628659 41.0 38.0 41.0 32.0 41.0 34-35 37.26783791299238 40.0 38.0 41.0 31.0 41.0 36-37 37.18893470952645 40.0 38.0 41.0 31.0 41.0 38-39 37.10284918670554 40.0 38.0 41.0 31.0 41.0 40-41 36.97262599472975 40.0 37.5 41.0 31.0 41.0 42-43 36.8058379995619 40.0 37.0 41.0 30.0 41.0 44-45 36.62706701137078 40.0 37.0 41.0 30.0 41.0 46-47 36.39383297065949 40.0 36.5 41.0 29.5 41.0 48-49 36.177517697565435 40.0 36.0 41.0 29.0 41.0 50-51 35.91939786960268 40.0 35.5 41.0 28.0 41.0 52-53 35.79233380946572 40.0 35.0 41.0 28.0 41.0 54-55 35.65636522108676 39.5 35.0 41.0 28.0 41.0 56-57 35.457122507683046 39.0 35.0 41.0 27.0 41.0 58-59 35.26441284219556 39.0 35.0 41.0 27.5 41.0 60-61 35.00683833665839 38.5 35.0 41.0 27.0 41.0 62-63 34.64686257612544 37.5 35.0 40.5 26.0 41.0 64-65 34.35521705996776 37.0 35.0 40.0 26.0 41.0 66-67 34.050333292671894 36.5 35.0 39.5 26.0 41.0 68-69 33.682417729438754 36.0 34.5 39.0 26.0 41.0 70-71 33.3160502522977 35.5 34.0 39.0 25.0 40.5 72-73 32.99494552022382 35.0 34.0 37.5 25.0 39.5 74-75 32.673138722417434 35.0 34.0 37.0 25.0 39.0 76-77 32.3543359527173 35.0 34.0 36.5 24.0 39.0 78-79 32.09975596575519 35.0 34.0 36.0 24.0 38.0 80-81 31.857100930884727 35.0 34.0 36.0 23.0 37.0 82-83 31.6395320392501 35.0 33.5 35.0 23.0 37.0 84-85 31.433383438725173 35.0 33.0 35.0 21.0 36.0 86-87 31.30816258806154 35.0 33.0 35.0 20.0 36.0 88-89 31.212993495480674 35.0 33.0 35.0 20.0 36.0 90-91 31.11155991726052 35.0 33.0 35.0 20.0 35.5 92-93 31.03750526303363 35.0 33.0 35.0 20.0 35.0 94-95 30.972686003150606 35.0 33.0 35.0 19.5 35.0 96-97 30.905863183423772 35.0 33.0 35.0 19.0 35.0 98-99 30.832817851160755 35.0 33.0 35.0 18.5 35.0 100-101 30.764808307526433 35.0 33.0 35.0 18.0 35.0 102-103 30.075446652841777 34.0 31.5 35.0 17.0 35.0 104-105 30.590322838745898 35.0 33.0 35.0 17.0 35.0 106-107 30.688191326520574 35.0 33.0 35.0 17.0 35.0 108-109 30.676251618259876 35.0 33.0 35.0 16.0 35.0 110-111 30.610756853748658 35.0 33.0 35.0 15.0 35.0 112-113 30.57272856650048 35.0 33.0 35.0 10.0 35.0 114-115 30.449734585705798 35.0 33.0 35.0 7.0 35.0 116-117 30.632906847255942 35.0 33.0 35.0 4.5 35.0 118-119 30.616728511410784 35.0 33.0 35.0 2.0 35.0 120-121 30.539317648509364 35.0 33.0 35.0 2.0 35.0 122-123 30.401489454913673 35.0 33.0 35.0 2.0 35.0 124-125 29.765132615330938 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31385.0 3 8309.0 4 4287.0 5 3244.0 6 3536.0 7 3746.0 8 4188.0 9 4295.0 10 4673.0 11 5167.0 12 5779.0 13 7544.0 14 10181.0 15 9560.0 16 5085.0 17 4510.0 18 3990.0 19 3624.0 20 3310.0 21 3388.0 22 3819.0 23 4566.0 24 5883.0 25 7943.0 26 9541.0 27 10828.0 28 13046.0 29 15793.0 30 19010.0 31 23951.0 32 30869.0 33 42267.0 34 66016.0 35 117401.0 36 269887.0 37 560452.0 38 193398.0 39 315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22387021864197 18.270084819700735 11.649884217500542 26.856160744156753 2 15.616101093544845 19.764278584386062 37.6717476648876 26.94787265718149 3 18.53319670302852 23.79814315741963 28.65293628321835 29.015723856333498 4 13.314843779252591 15.21847518986061 34.05351504071796 37.41316599016883 5 14.550001115173842 37.62616224073848 32.34614208889116 15.477694555196514 6 34.456235179999226 34.03679593306036 16.421988303479488 15.084980583460927 7 30.148997920577752 32.01542538916168 19.1325876110004 18.702989079260174 8 28.502676007267247 34.45465156790996 18.19842411811831 18.844248306704486 9 28.292667245314558 15.196067818582732 18.190341041421874 38.32092389468084 10-11 26.992337937920595 25.15342335132446 25.868704458210413 21.985534252544532 12-13 26.903998986817474 22.485794774104264 26.60040514177009 24.009801097308177 14-15 24.45453576028021 23.628378544652158 24.238768261572154 27.678317433495476 16-17 24.421283879216414 26.739166738025006 24.797924669873364 24.041624712885216 18-19 24.360745340310906 25.78386994236055 26.799965752896654 23.05541896443189 20-21 25.09638123476875 24.743688562497745 26.72385800454601 23.436072198187503 22-23 24.996687972947626 24.206556370700433 26.526319793591853 24.270435862760092 24-25 24.478772809940715 24.537078635473158 26.52478915787106 24.459359396715065 26-27 24.084490553626082 24.828486983177772 26.761769313229777 24.325253149966372 28-29 24.231308422707812 25.22388573621015 26.598697791558106 23.94610804952393 30-31 24.930972562167327 24.773570328629628 26.478990736878554 23.81646637232449 32-33 24.50344243618359 24.736971902348543 26.522106428570027 24.237479232897844 34-35 24.478831052097057 25.049886159537987 26.604661900550365 23.866620887814594 36-37 24.577168561640175 24.91667986605815 26.587903192219542 23.918248380082137 38-39 24.590402080330804 25.070412836139518 26.487293881158152 23.851891202371526 40-41 24.573624726798133 24.86777275507176 26.47063356599557 24.087968952134542 42-43 24.453898160241906 24.807926080439366 26.28939323038016 24.44878252893857 44-45 24.3141363660213 24.914476100529935 26.378121884673906 24.393265648774857 46-47 24.328579439436222 24.95138435707657 26.184795191232457 24.53524101225475 48-49 24.38829556991414 25.290407120877045 25.925689093861575 24.39560821534724 50-51 24.375518727305938 25.363375548567962 25.94090697881084 24.320198745315263 52-53 24.572157485788864 25.260358512499405 25.69871898922626 24.46876501248547 54-55 24.202505128837444 25.023578223297253 26.01517532491382 24.758741322951483 56-57 24.38685195745338 25.2166467894105 25.721770661825733 24.674730591310386 58-59 24.37930985860532 25.21303969865708 25.93216242795246 24.47548801478514 60-61 24.718878214881585 25.148316089360854 26.04008174272553 24.092723953032035 62-63 24.488440078765848 25.234977676997715 25.95577373017942 24.320808514057017 64-65 24.29940988440345 24.915102840660918 26.0843488856797 24.701138389255934 66-67 24.416258835967454 25.12644828540585 25.818503424203826 24.63878945442287 68-69 24.243887946672753 25.15717083318577 25.938040653893623 24.66090056624785 70-71 24.164702456607028 25.092111286436257 26.048343833167408 24.694842423789307 72-73 24.36269290387869 25.411188875855835 25.630174805075306 24.59594341519017 74-75 24.226175816946956 25.39416343956401 25.705827935881853 24.673832807607184 76-77 24.274337806707603 24.957591756093162 25.744107688902368 25.023962748296867 78-79 24.088895243160753 25.078084855981192 25.94758534572285 24.885434555135205 80-81 24.288654276731293 25.32175006853421 25.560734424371685 24.82886123036282 82-83 24.363435908097873 25.279938663705664 25.678604901000778 24.67802052719568 84-85 24.247693819656384 25.4077768883855 25.406432415823723 24.9380968761344 86-87 24.02786358216825 25.475738890572185 25.60293706789018 24.89346045936938 88-89 23.908245893973586 25.15525282157124 25.734782468974025 25.201718815481144 90-91 24.1729266107633 25.01095244278824 25.79096967812935 25.025151268319107 92-93 24.158570448574423 25.21878479996537 25.644287132751742 24.97835761870846 94-95 24.17109556819087 25.07108595998593 25.64157722783284 25.116241243990363 96-97 23.922087640419225 24.993171076582644 25.726989315399194 25.35775196759893 98-99 23.87708096140023 25.040104251378565 25.539849787772106 25.542964999449097 100-101 23.82737643184027 25.195010972031483 25.644483881672574 25.333128714455665 102-103 23.83481110349244 24.98230053382049 25.79173753831332 25.39115082437375 104-105 23.940605435779187 25.13615025321586 25.543223770417615 25.380020540587335 106-107 23.933048548426605 24.993039963561873 25.68550210473469 25.388409383276827 108-109 23.913059874386004 24.947720955002143 25.64012931951818 25.49908985109367 110-111 23.67042687015772 25.34177780532265 25.649559003255867 25.338236321263757 112-113 23.63662477652543 25.355526232602433 25.58139458624249 25.426454404629645 114-115 23.62406921364703 25.438323804127265 25.516957789486526 25.420649192739177 116-117 23.550878974107736 25.213336783055436 25.610022032968455 25.62576220986837 118-119 23.636572910405402 24.97974260067952 25.683004738804975 25.7006797501101 120-121 23.48656386733436 25.068757150760074 25.84390036271508 25.600778619190486 122-123 23.358126192395297 25.27254983876225 25.552723793209058 25.816600175633393 124-125 23.240649586105675 25.200398252442547 25.48086881534342 26.078083346108354 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 69.0 1 48.5 2 41.5 3 71.5 4 68.0 5 70.0 6 181.0 7 480.0 8 828.5 9 952.5 10 800.0 11 620.0 12 627.0 13 717.5 14 804.0 15 834.0 16 897.5 17 1025.0 18 1089.5 19 1176.5 20 1260.0 21 1281.5 22 1395.5 23 1565.0 24 1759.5 25 2075.5 26 2608.0 27 3384.5 28 4236.5 29 5345.0 30 6914.5 31 8654.5 32 10806.5 33 12935.0 34 15042.0 35 17474.0 36 20487.0 37 23567.5 38 25925.5 39 28673.5 40 31816.5 41 34681.5 42 37677.0 43 41563.0 44 45756.0 45 50059.0 46 54309.0 47 58511.5 48 63359.5 49 67747.0 50 70993.0 51 72287.5 52 71070.0 53 69826.5 54 69299.5 55 67564.0 56 63992.0 57 58422.0 58 52721.5 59 47039.5 60 40313.0 61 34326.0 62 28555.5 63 21854.5 64 15961.0 65 11953.0 66 8961.0 67 6722.0 68 5085.5 69 3835.0 70 2973.0 71 2305.0 72 1768.5 73 1425.0 74 1095.0 75 792.5 76 575.0 77 351.0 78 204.0 79 119.0 80 69.0 81 36.5 82 17.5 83 12.5 84 9.0 85 4.5 86 2.0 87 1.0 88 2.0 89 1.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024659198077631877 2 0.0013116594722144617 3 0.03134866138592563 4 0.16297368942264687 5 0.023609870499860307 6 0.07115752636763453 7 0.05279429375663208 8 0.045055502870566755 9 0.03561155467062263 10-11 0.06423852265170325 12-13 0.05738510190938269 14-15 0.06056587612950276 16-17 0.05164659171844442 18-19 0.03734950347130679 20-21 0.024331283209578263 22-23 0.0026233189444289234 24-25 0.004394059231918446 26-27 0.003115191246509346 28-29 0.0044924336923345305 30-31 0.001967489208321692 32-33 0.009116033331890509 34-35 0.004230101797891638 36-37 0.006197591006213331 38-39 0.0018691147479056077 40-41 0.002852859352066454 42-43 0.003115191246509346 44-45 0.004328476258307723 46-47 0.004656391126361338 48-49 0.001967489208321692 50-51 0.001344450959019823 52-53 8.853701437447616E-4 54-55 0.004853140047193508 56-57 7.542041965233154E-4 58-59 7.542041965233154E-4 60-61 1.3116594722144614E-4 62-63 8.853701437447616E-4 64-65 0.0 66-67 0.0034103146277575996 68-69 7.86995683328677E-4 70-71 0.0 72-73 0.002000280695127054 74-75 0.0045580166659452545 76-77 0.0015411998798519923 78-79 1.6395743402680771E-4 80-81 0.0 82-83 0.005049888968025677 84-85 0.0016067828534627154 86-87 0.0 88-89 9.83744604160846E-4 90-91 7.86995683328677E-4 92-93 0.0 94-95 0.003115191246509346 96-97 0.0014100339326305461 98-99 6.558297361072309E-4 100-101 0.0 102-103 0.0014756169062412692 104-105 0.0 106-107 6.230382493018693E-4 108-109 0.002328195563180669 110-111 9.837446041608463E-5 112-113 2.623318944428923E-4 114-115 0.0 116-117 0.0016723658270734385 118-119 0.002262612589569946 120-121 0.002328195563180669 122-123 0.0012460764986037386 124-125 0.10699862144589471 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1524786.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.22529520607879 #Duplication Level Percentage of deduplicated Percentage of total 1 80.43469154400205 49.24637734591415 2 10.663719974294771 13.057788068423125 3 3.4371759964551503 6.313263451746439 4 1.6358621116593757 4.0062456281113885 5 0.9118740711208271 2.791487959757076 6 0.5950462891829958 2.1859130829906395 7 0.42684821783741095 1.8293735701698879 8 0.3042470060266865 1.4902090207639611 9 0.2508880573168205 1.382462583561176 >10 1.2745046544271161 13.611869619404002 >50 0.05092106307378269 2.0621400068705484 >100 0.01335920615276294 1.2846946320894213 >500 5.38630281463828E-4 0.2587438030260578 >1k 3.231781688782968E-4 0.47943122717216774 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3039 0.19930665680298743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2487 0.1631048553698683 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1743 0.11431112300349033 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.558297361072307E-5 2 0.0 0.0 0.0 0.0 6.558297361072307E-5 3 0.0 0.0 0.0 0.0 6.558297361072307E-5 4 0.0 0.0 0.0 0.0 6.558297361072307E-5 5 0.0 0.0 0.0 0.0 6.558297361072307E-5 6 0.0 0.0 0.0 0.0 6.558297361072307E-5 7 0.0 0.0 0.0 0.0 6.558297361072307E-5 8 0.0 0.0 0.0 0.0 6.558297361072307E-5 9 0.0 0.0 0.0 0.0 1.3116594722144614E-4 10-11 0.0 0.0 0.0 0.0 1.3116594722144614E-4 12-13 0.0 0.0 0.0 9.83744604160846E-5 3.607063548589769E-4 14-15 0.0 0.0 0.0 1.3116594722144614E-4 5.246637888857846E-4 16-17 6.558297361072307E-5 0.0 0.0 1.639574340268077E-4 5.902467624965077E-4 18-19 6.558297361072307E-5 0.0 0.0 1.9674892083216923E-4 5.902467624965077E-4 20-21 6.558297361072307E-5 0.0 0.0 1.9674892083216923E-4 5.902467624965077E-4 22-23 6.558297361072307E-5 0.0 0.0 2.2954040763753075E-4 6.886212229125923E-4 24-25 6.558297361072307E-5 0.0 0.0 5.574552756911461E-4 7.869956833286769E-4 26-27 6.558297361072307E-5 0.0 0.0 9.509531173554845E-4 7.869956833286769E-4 28-29 6.558297361072307E-5 0.0 0.0 0.0025249444840128384 7.869956833286769E-4 30-31 6.558297361072307E-5 0.0 0.0 0.01088677361938003 8.197871701340385E-4 32-33 6.558297361072307E-5 0.0 0.0 0.02843021906024845 9.181616305501231E-4 34-35 6.558297361072307E-5 0.0 0.0 0.049154438721236954 9.83744604160846E-4 36-37 6.558297361072307E-5 0.0 0.0 0.08037193415994114 9.83744604160846E-4 38-39 6.558297361072307E-5 0.0 0.0 0.13083803235339253 0.0011477020381876538 40-41 6.558297361072307E-5 0.0 0.0 0.18976433414262722 0.0011804935249930153 42-43 6.558297361072307E-5 0.0 0.0 0.24285375128050757 0.0011804935249930153 44-45 6.558297361072307E-5 0.0 0.0 0.3015833041489101 0.0011804935249930153 46-47 6.558297361072307E-5 0.0 0.0 0.36896980953392805 0.0012788679854091 48-49 6.558297361072307E-5 0.0 0.0 0.43861892750851594 0.0013116594722144615 50-51 6.558297361072307E-5 0.0 0.0 0.5099076198233718 0.0013116594722144615 52-53 6.558297361072307E-5 0.0 0.0 0.5878857754465217 0.0015739913666573538 54-55 6.558297361072307E-5 0.0 0.0 0.6737994708765689 0.001639574340268077 56-57 6.558297361072307E-5 0.0 0.0 0.7613527406468843 0.001639574340268077 58-59 6.558297361072307E-5 0.0 0.0 0.8583499586171437 0.0016723658270734385 60-61 6.558297361072307E-5 0.0 0.0 0.9575769976901677 0.0017051573138788 62-63 6.558297361072307E-5 0.0 0.0 1.0693959676964506 0.0017051573138788 64-65 6.558297361072307E-5 0.0 0.0 1.1753124700777682 0.0017051573138788 66-67 6.558297361072307E-5 0.0 0.0 1.2970344690992703 0.0017051573138788 68-69 6.558297361072307E-5 0.0 0.0 1.4365622454560838 0.0017051573138788 70-71 6.558297361072307E-5 0.0 0.0 1.5768442260094204 0.0017379488006841616 72-73 1.3116594722144614E-4 0.0 0.0 1.7117484027266778 0.0020002806951270537 74-75 1.3116594722144614E-4 0.0 0.0 1.8540306639751414 0.0020330721819324152 76-77 1.3116594722144614E-4 0.0 0.0 2.0245463953630214 0.0020330721819324152 78-79 1.3116594722144614E-4 0.0 0.0 2.2112283297459445 0.0020330721819324152 80-81 1.3116594722144614E-4 0.0 0.0 2.398664468325391 0.0020330721819324152 82-83 1.639574340268077E-4 0.0 0.0 2.5911504958728635 0.0021642381291538614 84-85 1.9674892083216923E-4 0.0 0.0 2.791342522819596 0.0021642381291538614 86-87 1.9674892083216923E-4 0.0 0.0 3.0111110673891286 0.002197029615959223 88-89 1.9674892083216923E-4 0.0 0.0 3.268917736652881 0.0022298211027645845 90-91 1.9674892083216923E-4 0.0 0.0 3.5352501924860276 0.0022298211027645845 92-93 1.9674892083216923E-4 0.0 0.0 3.7992872442427985 0.0022298211027645845 94-95 1.9674892083216923E-4 0.0 0.0 4.066242738325247 0.0022954040763753076 96-97 1.9674892083216923E-4 0.0 0.0 4.372482433600519 0.0023609870499860307 98-99 1.9674892083216923E-4 0.0 0.0 4.714530432467244 0.0024265700235967537 100-101 2.2954040763753075E-4 0.0 0.0 5.080942506030355 0.0024265700235967537 102-103 2.623318944428923E-4 0.0 0.0 5.458011812805207 0.0024593615104021153 104-105 2.623318944428923E-4 0.0 0.0 5.848427254709842 0.002492152997207477 106-107 2.9512338124825383E-4 0.0 0.0 6.262977230903221 0.002492152997207477 108-109 3.279148680536154E-4 0.0 0.0 6.7000877500186915 0.0025577359708182 110-111 3.279148680536154E-4 0.0 0.0 7.188418571524135 0.0026561104312342845 112-113 3.279148680536154E-4 0.0 0.0 7.676224729240694 0.002688901918039646 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3435 0.0 43.421524 1 GTATCAA 5195 0.0 40.424896 1 TCAACGC 6455 0.0 32.43096 4 ATGGGGG 4310 0.0 32.351013 5 CATGGGG 7290 0.0 32.316067 4 GTACATG 9210 0.0 32.065453 1 CAACGCA 6595 0.0 31.713312 5 TATCAAC 6630 0.0 31.309187 2 ATCAACG 6795 0.0 30.812273 3 TACATGG 9550 0.0 30.667912 2 AACGCAG 6810 0.0 30.63163 6 ACATGGG 9675 0.0 29.77846 3 ACGCAGA 8165 0.0 25.619555 7 TGGGGGG 3095 0.0 25.419233 6 TATCACG 165 1.0887574E-5 25.305809 2 CGCAGAG 8540 0.0 24.353403 8 GTATAGG 495 0.0 22.900248 1 GAGTACT 3815 0.0 22.863888 12-13 GCAGAGT 9235 0.0 22.456106 9 GTACTTT 4030 0.0 21.932026 14-15 >>END_MODULE