FastQCFastQC Report
Mon 15 Aug 2016
SRR2921625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177853
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG1750.068.0095143
GCGTAGC3600.044.6312452
CGCGTAG853.4715176E-642.016591
GTACATG67200.040.4795271
GGTATCA16100.038.8196751
GTATCAA22800.038.3769951
TACATGG70250.038.0345042
ACATGGG72000.036.0356033
CATGGGG61200.035.1021354
GTAGCGT2750.034.6230284
ATGGGGG38400.034.5567555
TCAACGC26900.031.8557594
ACCGTAT1352.3365665E-630.8417558
CAACGCA28250.030.3321595
GGCGTAG3850.029.3752371
TATCAAC29650.029.3025842
ATCAACG29500.028.6446883
AACGCAG30150.028.6168296
TAGCGTC2853.6379788E-1227.1429965
TGGGGGG30750.026.7039076
Produced by FastQC (version 0.11.5)