##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921625_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1177853
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.539243012498165	31.0	30.0	33.0	26.0	34.0
2	29.70979655355974	31.0	31.0	34.0	26.0	34.0
3	30.054472841687375	31.0	31.0	34.0	26.0	34.0
4	33.45433598250376	37.0	35.0	37.0	28.0	37.0
5	33.72872251460921	37.0	35.0	37.0	30.0	37.0
6	33.644221307752325	37.0	35.0	37.0	28.0	37.0
7	33.48376919700506	37.0	35.0	37.0	28.0	37.0
8	33.57191262407108	37.0	35.0	37.0	28.0	37.0
9	35.175169567000296	39.0	37.0	39.0	28.0	39.0
10-11	35.240502422628296	39.0	37.0	39.0	28.0	39.0
12-13	35.183454981224315	39.0	37.0	39.0	27.5	39.0
14-15	36.46875246741317	40.0	38.0	41.0	27.0	41.0
16-17	36.44092726341912	40.0	38.0	41.0	27.0	41.0
18-19	36.38860367125609	40.0	38.0	41.0	27.0	41.0
20-21	36.46432492000275	40.0	38.0	41.0	27.0	41.0
22-23	36.41081102650331	40.0	38.0	41.0	27.0	41.0
24-25	36.416737063113985	40.0	38.0	41.0	27.0	41.0
26-27	36.3923940423805	40.0	38.0	41.0	28.0	41.0
28-29	36.34999274102965	40.0	38.0	41.0	28.0	41.0
30-31	36.25564353106881	40.0	37.0	41.0	27.5	41.0
32-33	36.16705862276532	40.0	37.0	41.0	27.5	41.0
34-35	36.05222892839768	40.0	37.0	41.0	27.0	41.0
36-37	35.94889727325906	40.0	37.0	41.0	27.0	41.0
38-39	35.83876171304908	40.0	36.5	41.0	26.0	41.0
40-41	35.727383637856335	40.0	36.0	41.0	25.5	41.0
42-43	35.48699965106002	40.0	35.5	41.0	24.5	41.0
44-45	35.15558435560295	40.0	35.0	41.0	24.0	41.0
46-47	34.92669883253683	39.5	35.0	41.0	23.0	41.0
48-49	34.74432123533242	39.0	35.0	41.0	23.0	41.0
50-51	34.550060151818606	39.0	35.0	41.0	22.0	41.0
52-53	34.35475734238483	39.0	34.0	40.5	22.0	41.0
54-55	34.13041143504326	38.0	34.0	40.0	20.5	41.0
56-57	33.88510068743723	38.0	34.0	40.0	19.5	41.0
58-59	33.9026763102017	38.0	34.0	40.0	20.0	41.0
60-61	33.69578843879499	37.5	34.0	40.0	19.0	41.0
62-63	33.37779035244636	37.0	34.0	40.0	18.0	41.0
64-65	33.08275862947244	36.5	33.0	39.5	15.5	41.0
66-67	32.762933490002574	36.0	33.0	39.0	13.0	41.0
68-69	32.422359156872716	35.5	33.0	39.0	10.5	40.5
70-71	32.05659449863438	35.0	33.0	38.5	7.5	40.0
72-73	31.703326306423634	35.0	32.0	37.0	7.0	39.5
74-75	31.334796022933254	35.0	32.0	37.0	4.0	39.0
76-77	31.027333207114978	35.0	32.0	36.0	2.0	39.0
78-79	30.738892289615087	35.0	32.0	36.0	2.0	37.5
80-81	30.476485605589154	35.0	31.5	35.5	2.0	37.0
82-83	30.248437198869468	35.0	31.0	35.0	2.0	37.0
84-85	30.02577443874575	35.0	31.0	35.0	2.0	36.0
86-87	29.847773024307788	35.0	31.0	35.0	2.0	36.0
88-89	29.68232665706162	35.0	31.0	35.0	2.0	36.0
90-91	29.500359128006636	34.5	31.0	35.0	2.0	35.0
92-93	29.41572972179041	34.0	31.0	35.0	2.0	35.0
94-95	29.28547535218741	34.0	30.0	35.0	2.0	35.0
96-97	29.189716798276187	34.0	30.0	35.0	2.0	35.0
98-99	29.09665255341711	34.0	30.0	35.0	2.0	35.0
100-101	28.986615901984372	34.0	30.0	35.0	2.0	35.0
102-103	28.115923209432758	33.5	28.0	34.5	2.0	35.0
104-105	29.015805452802685	34.0	30.5	35.0	2.0	35.0
106-107	29.276506066546503	35.0	31.0	35.0	2.0	35.0
108-109	29.269435999229106	35.0	31.0	35.0	2.0	35.0
110-111	29.21045453040405	35.0	31.0	35.0	2.0	35.0
112-113	29.12176859081736	35.0	31.0	35.0	2.0	35.0
114-115	29.0048265785289	35.0	31.0	35.0	2.0	35.0
116-117	28.885387650241583	35.0	31.0	35.0	2.0	35.0
118-119	28.597779179575042	35.0	30.0	35.0	2.0	35.0
120-121	28.467512499437536	34.0	30.0	35.0	2.0	35.0
122-123	28.235608348410203	34.0	30.0	35.0	2.0	35.0
124-125	27.494820660982313	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50716.0
3	8414.0
4	4230.0
5	3511.0
6	3822.0
7	4048.0
8	4249.0
9	4469.0
10	4673.0
11	5068.0
12	5129.0
13	5226.0
14	5311.0
15	4762.0
16	4062.0
17	3726.0
18	3434.0
19	3195.0
20	3032.0
21	3106.0
22	3605.0
23	5038.0
24	7804.0
25	10186.0
26	10487.0
27	11324.0
28	13382.0
29	16321.0
30	19243.0
31	24350.0
32	31948.0
33	43969.0
34	68862.0
35	112253.0
36	223024.0
37	348160.0
38	93617.0
39	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.564608924196556	17.686621347928174	11.976799976222523	24.771969751652748
2	17.036511237133272	19.39839371274136	38.9246586915526	24.640436358572767
3	20.368919096763786	23.787088256146173	27.842396403357206	28.001596243732834
4	14.057784955329927	14.906185204619671	34.55447948878621	36.4815503512642
5	14.524866898197288	38.08673057817555	32.68818939091596	14.700213132711202
6	34.203218498634456	34.63777869550365	16.614322301928414	14.544680503933474
7	30.86606320696697	31.910225176094027	19.118306643153485	18.10540497378552
8	27.698653221532147	35.4147697158311	18.423328078496393	18.46324898414036
9	28.239327358867904	16.370278718748065	17.913120867007994	37.477273055376045
10-11	26.798845993275233	25.889299106124703	26.064574601234447	21.24728029936562
12-13	26.262977482629466	23.163453235210955	26.537280305133766	24.036288977025812
14-15	24.1199377178527	24.445530508613476	23.695580082111547	27.738951691422276
16-17	24.64406857391331	27.48239026933928	24.295186135744824	23.578355021002587
18-19	24.62419753002534	26.87240870729556	25.621241391376877	22.882152371302226
20-21	25.2977638729303	25.73202143135571	25.576441134683108	23.393773561030873
22-23	25.32325055724069	25.191228358487194	25.374080590019055	24.111440494253063
24-25	24.832357826868268	25.45057018720892	25.397746766900948	24.319325219021867
26-27	24.38059014711422	25.744500044803992	25.60758244889691	24.267327359184883
28-29	24.516354796871987	26.271763715631824	25.3128394774415	23.899042010054693
30-31	25.264390588556996	25.630229800185383	25.437714133341938	23.667665477915683
32-33	24.705258907200026	25.723503662156748	25.437265634918916	24.133971795724307
34-35	24.674570737441478	26.13681533726074	25.44646193883306	23.742151986464723
36-37	24.799904224034247	25.9965518954174	25.43526907484917	23.76827480569918
38-39	24.863007707912818	26.062077697323673	25.395819198197046	23.679095396566467
40-41	24.711128777387508	25.807754391195058	25.49252177177464	23.98859505964279
42-43	24.56133274343347	25.790569131649853	25.350946543014146	24.29715158190253
44-45	24.309559514084782	25.878101717482384	25.729925767670302	24.082413000762536
46-47	24.231041296740234	25.724885975616484	25.615638260562378	24.4284344670809
48-49	24.258339723844973	25.99685612304431	25.57881899947942	24.165985153631297
50-51	24.303756834197568	26.01169053292729	25.654460932762873	24.03009170011227
52-53	24.3848535838799	25.994163481123028	25.533772282352324	24.087210652644757
54-55	24.12494283898235	25.59246837514951	25.810624964705593	24.47196382116255
56-57	24.236326742565165	25.79091128724424	25.510378687165176	24.46238328302542
58-59	24.29271308192619	25.975256686719288	25.564099396128654	24.16793083522587
60-61	24.763235561986747	25.823192958187217	25.719549471401233	23.6940220084248
62-63	24.499214078816305	25.81239454177526	25.744502160752514	23.943889218655926
64-65	24.246936413436217	25.609214493942456	25.713195139700908	24.430653952920416
66-67	24.293328792815224	25.807113185859908	25.5227080397318	24.376849981593068
68-69	24.117174261302576	25.879620056615316	25.720822030296453	24.28238365178565
70-71	24.12106227191786	25.697491261054004	25.875264206865534	24.306182260162597
72-73	24.156368326883868	26.161986452301555	25.488380347770118	24.193264873044463
74-75	24.14941698202761	26.027148353089913	25.652408700990275	24.1710259638922
76-77	24.180340919077892	25.59419259817801	25.691376060940698	24.534090421803402
78-79	24.091341991525802	25.630522739750223	25.927106947373606	24.35102832135037
80-81	24.308813576880716	25.95633333871128	25.46352945572068	24.271323628687323
82-83	24.410626659380572	26.01216642018775	25.584256939305046	23.992949981126635
84-85	24.35724623437295	26.132702399667835	25.31158576425491	24.198465601704303
86-87	24.035450321318425	26.19844809314838	25.560778772604376	24.20532281292882
88-89	23.933624236104244	25.85490725404287	25.741201266815473	24.47026724303742
90-91	24.371303172642662	25.623962680486695	25.66990301155429	24.334831135316353
92-93	24.37559222793176	25.764886784699037	25.682993761016714	24.176527226352487
94-95	24.351248679656692	25.698635673553394	25.60757019471455	24.34254545207536
96-97	24.035393255564745	25.674715245711493	25.752028136627715	24.53786336209605
98-99	23.910574728161084	25.739206998337107	25.62369164320062	24.726526630301194
100-101	23.901781086006928	25.88753936045966	25.761704761633865	24.44897479189955
102-103	23.745449682777885	25.84817913020222	25.871612600584566	24.53475858643533
104-105	23.877556305259347	25.87123349197677	25.712462700538335	24.53874750222555
106-107	23.909820014441635	25.79542545726537	25.78638351666965	24.50837101162335
108-109	23.87483256594938	25.79416278729136	25.842816840415978	24.488187806343284
110-111	23.634125323156468	26.1941613214075	25.79419018792955	24.377523167506485
112-113	23.599643410523637	26.31532697981449	25.781928554751342	24.303101054910535
114-115	23.720036103995128	26.358845860904516	25.632223240206198	24.288894794894155
116-117	23.52855521767869	26.30097569196951	25.682481313352795	24.487987776999002
118-119	23.608759208602898	26.08938847129317	25.78289619872959	24.518956121374348
120-121	23.586854778970874	26.131804549419808	25.96191765877017	24.319423012839152
122-123	23.48077356561074	26.35071947103374	25.749876150884095	24.418630812471427
124-125	23.364100747241068	26.26700539778195	25.793102625989413	24.57579122898757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	471.0
1	382.0
2	252.0
3	193.0
4	167.0
5	152.5
6	157.0
7	179.0
8	204.5
9	239.0
10	251.0
11	241.0
12	268.0
13	301.5
14	309.5
15	346.5
16	401.0
17	443.5
18	495.0
19	546.5
20	610.5
21	706.0
22	844.5
23	1057.5
24	1358.0
25	1715.0
26	2186.5
27	2878.0
28	3725.0
29	4834.0
30	6251.0
31	7572.5
32	9258.0
33	11126.5
34	12953.0
35	14801.5
36	16955.0
37	19289.0
38	21624.5
39	24136.0
40	26518.0
41	28728.0
42	30738.5
43	33817.0
44	37250.5
45	40729.5
46	43883.0
47	46720.5
48	50459.0
49	53813.0
50	55377.0
51	55312.5
52	53882.5
53	52595.5
54	51746.0
55	49480.0
56	46199.0
57	42522.5
58	38546.5
59	33711.0
60	28290.0
61	23738.0
62	19817.5
63	15445.0
64	11333.5
65	8625.0
66	6691.5
67	5108.5
68	3979.0
69	3053.5
70	2385.5
71	1813.5
72	1373.0
73	1131.5
74	892.0
75	709.5
76	512.0
77	311.0
78	219.5
79	160.5
80	109.0
81	82.5
82	68.5
83	51.5
84	43.0
85	36.0
86	30.0
87	26.0
88	22.5
89	18.0
90	13.0
91	14.0
92	15.0
93	11.5
94	9.0
95	9.0
96	8.0
97	8.0
98	6.0
99	2.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022753263777398367
2	0.008999425225388906
3	0.0074712209418323
4	0.009084325463364275
5	0.015536743549492171
6	0.08753214535260342
7	0.05442105254221028
8	0.0445726249370677
9	0.024281468060954974
10-11	0.04784128409911933
12-13	0.011886033316551387
14-15	0.026191723415400735
16-17	0.03217719019266411
18-19	0.040115362443360926
20-21	0.027677477579969655
22-23	0.03391764507115913
24-25	0.029290582101501633
26-27	0.04266236958262194
28-29	0.04177091708388059
30-31	0.024323918179942658
32-33	0.028568930078711013
34-35	0.041176615418053016
36-37	0.008872074868425856
38-39	0.019696855210285157
40-41	0.03977576149145946
42-43	0.021394859969792495
44-45	0.01689514735709804
46-47	0.02156466044574323
48-49	0.027677477579969655
50-51	0.027083175914142086
52-53	0.022710813658410687
54-55	0.02194671151663238
56-57	0.023050414610312153
58-59	0.01710739795203646
60-61	0.02194671151663238
62-63	0.02156466044574323
64-65	0.019527054734334418
66-67	0.026106823177425364
68-69	0.02177691104068165
70-71	0.04317177101047414
72-73	0.020036456162186623
74-75	0.008659824273487438
76-77	0.015579193668479853
78-79	0.023644716276139722
80-81	0.017319648546974875
82-83	0.022710813658410687
84-85	0.010824780341859299
86-87	0.017404548784950246
88-89	0.021607110564730914
90-91	0.02024870675712504
92-93	0.008235323083610604
94-95	0.01095213069882235
96-97	0.014517940693787765
98-99	0.007428770822844616
100-101	0.00989087772413026
102-103	0.004287462017756036
104-105	0.004627062969657504
106-107	7.216520227906199E-4
108-109	0.01277748581529274
110-111	0.0015282042835566068
112-113	0.002377206663310277
114-115	0.012225634268452855
116-117	0.006240167491189478
118-119	0.277793578655401
120-121	8.91452498741354E-4
122-123	7.216520227906199E-4
124-125	0.31132917265567095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1177853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.15390627752848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.3278946680438	58.316033244551946
2	8.965976834240516	12.400646433631204
3	2.682477878251362	5.565114713524143
4	1.2910171117044968	3.571155053819932
5	0.7202041799508504	2.490246618050268
6	0.4688055020374495	1.945183905017248
7	0.3180151128734734	1.5394391117342876
8	0.22817300006510172	1.2623243409251634
9	0.1603326978778174	0.9978869126039285
>10	0.7997463371027318	9.495169647837614
>50	0.02901545112018628	1.3582374788316731
>100	0.007973233538880402	0.8873812041113368
>500	3.6799319333951145E-4	0.17118133536130475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.490023797536705E-5
7	0.0	0.0	0.0	0.0	8.490023797536705E-5
8	0.0	0.0	0.0	8.490023797536705E-5	8.490023797536705E-5
9	0.0	0.0	0.0	8.490023797536705E-5	8.490023797536705E-5
10-11	0.0	0.0	0.0	1.698004759507341E-4	8.490023797536705E-5
12-13	0.0	0.0	0.0	2.1225059493841763E-4	8.490023797536705E-5
14-15	0.0	4.2450118987683525E-5	0.0	2.5470071392610114E-4	8.490023797536705E-5
16-17	0.0	8.490023797536705E-5	0.0	2.5470071392610114E-4	8.490023797536705E-5
18-19	0.0	8.490023797536705E-5	0.0	2.9715083291378464E-4	8.490023797536705E-5
20-21	0.0	8.490023797536705E-5	0.0	3.820510708891517E-4	8.490023797536705E-5
22-23	0.0	8.490023797536705E-5	0.0	6.367517848152528E-4	2.9715083291378464E-4
24-25	0.0	8.490023797536705E-5	0.0	0.0013159536886181893	5.094014278522023E-4
26-27	0.0	8.490023797536705E-5	0.0	0.0019951555924211254	5.094014278522023E-4
28-29	0.0	8.490023797536705E-5	0.0	0.004924213802571289	5.094014278522023E-4
30-31	0.0	8.490023797536705E-5	0.0	0.01515469247860302	5.094014278522023E-4
32-33	0.0	8.490023797536705E-5	0.0	0.0371014039952354	5.094014278522023E-4
34-35	0.0	8.490023797536705E-5	0.0	0.06418457990937748	5.094014278522023E-4
36-37	0.0	8.490023797536705E-5	0.0	0.10013983069194543	5.094014278522023E-4
38-39	0.0	8.490023797536705E-5	0.0	0.1661497657177933	5.094014278522023E-4
40-41	0.0	1.2735035696305057E-4	0.0	0.24561638846273687	5.094014278522023E-4
42-43	0.0	1.698004759507341E-4	0.0	0.3137912795569566	5.094014278522023E-4
44-45	0.0	1.698004759507341E-4	0.0	0.38557443076512943	5.094014278522023E-4
46-47	0.0	1.698004759507341E-4	0.0	0.46066869125434157	5.094014278522023E-4
48-49	0.0	1.698004759507341E-4	0.0	0.5419606691157555	7.641021417783034E-4
50-51	0.0	1.698004759507341E-4	0.0	0.6286013619696176	7.641021417783034E-4
52-53	0.0	1.698004759507341E-4	0.0	0.7216944729096075	8.490023797536705E-4
54-55	0.0	1.698004759507341E-4	0.0	0.8237021088370111	9.339026177290375E-4
56-57	0.0	1.698004759507341E-4	0.0	0.9260068955973284	0.001146153212667455
58-59	0.0	1.698004759507341E-4	0.0	1.0399854650792586	0.0011886033316551386
60-61	0.0	1.698004759507341E-4	0.0	1.1579967958650188	0.0011886033316551386
62-63	0.0	1.698004759507341E-4	0.0	1.2987613904281774	0.0013584038076058728
64-65	0.0	1.698004759507341E-4	0.0	1.4285738542925135	0.0014008539265935563
66-67	0.0	1.698004759507341E-4	0.0	1.5720977065898716	0.0014857541645689233
68-69	0.0	1.698004759507341E-4	0.0	1.733280808386106	0.0015282042835566068
70-71	0.0	1.698004759507341E-4	0.0	1.8987089220811084	0.0017404548784950243
72-73	0.0	1.698004759507341E-4	0.0	2.0599344739963303	0.001867805235458075
74-75	0.0	1.698004759507341E-4	0.0	2.2292679986382	0.0019102553544457586
76-77	0.0	1.698004759507341E-4	0.0	2.410784707429535	0.001952705473433442
78-79	0.0	1.698004759507341E-4	0.0	2.620658095704642	0.001952705473433442
80-81	0.0	1.698004759507341E-4	0.0	2.8187728010201614	0.001952705473433442
82-83	0.0	1.698004759507341E-4	0.0	3.0293253911990714	0.002122505949384176
84-85	0.0	1.698004759507341E-4	0.0	3.2633528971781707	0.002122505949384176
86-87	0.0	2.1225059493841763E-4	0.0	3.515591504202986	0.0021649560683718594
88-89	0.0	2.5470071392610114E-4	0.0	3.8000497515394533	0.002249856306347227
90-91	0.0	2.5470071392610114E-4	0.0	4.099280640283634	0.00229230642533491
92-93	0.0	2.5470071392610114E-4	0.0	4.382040882860594	0.00229230642533491
94-95	0.0	2.5470071392610114E-4	0.0	4.677875762085762	0.002462106901285644
96-97	0.0	2.5470071392610114E-4	0.0	5.0073311355491725	0.002462106901285644
98-99	0.0	2.5470071392610114E-4	0.0	5.376774521098983	0.002462106901285644
100-101	0.0	2.5470071392610114E-4	0.0	5.769480571854043	0.002462106901285644
102-103	0.0	2.5470071392610114E-4	0.0	6.177001714135805	0.0026743574962240623
104-105	0.0	4.245011898768352E-4	0.0	6.598573845802489	0.0028017078531871126
106-107	0.0	4.245011898768352E-4	0.0	7.045446248385835	0.0028017078531871126
108-109	0.0	4.245011898768352E-4	0.0	7.51392576153391	0.0028441579721747963
110-111	0.0	4.245011898768352E-4	0.0	8.025407245216508	0.0029715083291378466
112-113	0.0	5.094014278522023E-4	0.0	8.517998425949589	0.0029715083291378466
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	175	0.0	68.009514	3
GCGTAGC	360	0.0	44.631245	2
CGCGTAG	85	3.4715176E-6	42.01659	1
GTACATG	6720	0.0	40.479527	1
GGTATCA	1610	0.0	38.819675	1
GTATCAA	2280	0.0	38.376995	1
TACATGG	7025	0.0	38.034504	2
ACATGGG	7200	0.0	36.035603	3
CATGGGG	6120	0.0	35.102135	4
GTAGCGT	275	0.0	34.623028	4
ATGGGGG	3840	0.0	34.556755	5
TCAACGC	2690	0.0	31.855759	4
ACCGTAT	135	2.3365665E-6	30.841755	8
CAACGCA	2825	0.0	30.332159	5
GGCGTAG	385	0.0	29.375237	1
TATCAAC	2965	0.0	29.302584	2
ATCAACG	2950	0.0	28.644688	3
AACGCAG	3015	0.0	28.616829	6
TAGCGTC	285	3.6379788E-12	27.142996	5
TGGGGGG	3075	0.0	26.703907	6
>>END_MODULE