FastQCFastQC Report
Mon 15 Aug 2016
SRR2921625_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921625_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177853
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.2222688230195109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.17863010070017227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15470.13134066814789283No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.056.6365471
GTATCAA32850.050.292071
TCAACGC41400.039.3193664
CAACGCA41800.038.901725
GTACATG64100.038.241851
ATCAACG43000.037.857933
TATCAAC43350.037.5570682
AACGCAG43300.037.4292536
TACATGG66600.036.3554842
ACATGGG67450.035.273913
CATGGGG57600.035.1965944
ATGGGGG33800.032.601495
ACGCAGA54100.029.8458357
CGCAGAG57600.028.02998
TGGGGGG25250.027.6092516
ATGGGGA20500.026.1499615
GCAGAGT62800.025.4243539
ATACCGA1501.6705577E-423.8336286
GAGTACA44600.023.8034691
GAGTACT32400.023.14992312-13
Produced by FastQC (version 0.11.5)