##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1177853
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.719645830167263	33.0	31.0	34.0	30.0	34.0
2	31.90383944346196	33.0	31.0	34.0	30.0	34.0
3	31.98673688482349	34.0	31.0	34.0	30.0	34.0
4	35.37865930638203	37.0	35.0	37.0	33.0	37.0
5	35.216255339163716	37.0	35.0	37.0	33.0	37.0
6	35.24482766525195	37.0	35.0	37.0	33.0	37.0
7	35.13655354275958	37.0	35.0	37.0	33.0	37.0
8	35.17206816130706	37.0	36.0	37.0	33.0	37.0
9	36.88574465574227	39.0	38.0	39.0	34.0	39.0
10-11	36.841636859608116	39.0	38.0	39.0	33.5	39.0
12-13	36.761075023793296	39.0	38.0	39.0	33.0	39.0
14-15	38.18412017458885	41.0	39.0	41.0	34.0	41.0
16-17	38.19045373234181	41.0	39.0	41.0	34.0	41.0
18-19	38.208289149834485	41.0	39.0	41.0	34.0	41.0
20-21	38.14972156966956	41.0	39.0	41.0	34.0	41.0
22-23	38.15249101543232	41.0	39.0	41.0	34.0	41.0
24-25	38.11094932899097	41.0	39.0	41.0	34.0	41.0
26-27	38.03560376379735	41.0	39.0	41.0	33.5	41.0
28-29	37.96390593732834	41.0	39.0	41.0	33.0	41.0
30-31	37.84279532335529	41.0	38.0	41.0	33.0	41.0
32-33	37.70971759633842	41.0	38.0	41.0	33.0	41.0
34-35	37.57270686579734	41.0	38.0	41.0	33.0	41.0
36-37	37.4996561540362	40.0	38.0	41.0	32.5	41.0
38-39	37.416682302460494	40.0	38.0	41.0	32.0	41.0
40-41	37.29719710354348	40.0	38.0	41.0	32.0	41.0
42-43	37.13568671132985	40.0	37.5	41.0	31.5	41.0
44-45	36.96618975372988	40.0	37.0	41.0	31.0	41.0
46-47	36.743111406941274	40.0	37.0	41.0	30.5	41.0
48-49	36.525134290951414	40.0	37.0	41.0	30.0	41.0
50-51	36.28305696890868	40.0	36.0	41.0	29.0	41.0
52-53	36.15970753566022	40.0	36.0	41.0	29.0	41.0
54-55	36.034149847222025	40.0	35.0	41.0	29.0	41.0
56-57	35.83983739906422	39.0	35.0	41.0	28.0	41.0
58-59	35.65224904975409	39.0	35.0	41.0	28.5	41.0
60-61	35.39976508104152	39.0	35.0	41.0	28.0	41.0
62-63	35.03912924617928	38.0	35.0	41.0	28.0	41.0
64-65	34.75247293168162	37.0	35.0	40.0	28.0	41.0
66-67	34.449705523524585	37.0	35.0	39.5	28.0	41.0
68-69	34.079199611496506	36.0	35.0	39.0	27.0	41.0
70-71	33.704585801454	36.0	34.5	39.0	26.0	41.0
72-73	33.38229048956025	35.0	34.0	37.5	26.0	40.0
74-75	33.056124575817186	35.0	34.0	37.0	26.0	39.0
76-77	32.723371252609624	35.0	34.0	37.0	26.0	39.0
78-79	32.460406774020186	35.0	34.0	36.0	26.0	38.5
80-81	32.20790455175646	35.0	34.0	36.0	25.0	37.0
82-83	31.97941211679216	35.0	34.0	35.5	25.0	37.0
84-85	31.76066538014506	35.0	34.0	35.0	24.5	36.5
86-87	31.62884290314666	35.0	34.0	35.0	24.0	36.0
88-89	31.530344194054777	35.0	33.5	35.0	24.0	36.0
90-91	31.42347984001399	35.0	33.0	35.0	24.0	36.0
92-93	31.34135796232637	35.0	33.0	35.0	24.0	35.0
94-95	31.271802593362672	35.0	33.0	35.0	23.0	35.0
96-97	31.207891392219572	35.0	33.0	35.0	23.0	35.0
98-99	31.131449764953693	35.0	33.0	35.0	21.5	35.0
100-101	31.062848674664835	35.0	33.0	35.0	20.0	35.0
102-103	30.372961651411508	34.0	31.5	35.0	19.5	35.0
104-105	30.888565466149004	35.0	33.0	35.0	20.0	35.0
106-107	30.981332984676357	35.0	33.0	35.0	20.0	35.0
108-109	30.96687489865034	35.0	33.0	35.0	19.5	35.0
110-111	30.901680005909057	35.0	33.0	35.0	19.0	35.0
112-113	30.86427296105711	35.0	33.0	35.0	19.0	35.0
114-115	30.734558981468822	35.0	33.0	35.0	17.5	35.0
116-117	30.906510404948662	35.0	33.0	35.0	18.0	35.0
118-119	30.8893244742765	35.0	33.0	35.0	16.5	35.0
120-121	30.809820495426848	35.0	33.0	35.0	6.0	35.0
122-123	30.678237861600728	35.0	33.0	35.0	2.0	35.0
124-125	30.035184781122943	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19408.0
3	5271.0
4	2889.0
5	2183.0
6	2305.0
7	2470.0
8	2816.0
9	3021.0
10	3318.0
11	3745.0
12	4224.0
13	5547.0
14	7645.0
15	7276.0
16	4173.0
17	3541.0
18	3222.0
19	2694.0
20	2583.0
21	2680.0
22	2802.0
23	3403.0
24	4472.0
25	5643.0
26	6937.0
27	7978.0
28	9761.0
29	11816.0
30	14266.0
31	18175.0
32	23414.0
33	31898.0
34	50544.0
35	88225.0
36	201494.0
37	439824.0
38	165771.0
39	419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52873640329386	18.06895989497131	11.489466135295771	26.912837566439052
2	15.64176276113612	19.689956309632766	37.83156200279496	26.836718926436156
3	18.900265137553905	23.60981127845218	28.54398548792274	28.94593809607117
4	13.177279368119205	14.840830738197733	34.20672189727515	37.77516799640791
5	14.249235950533581	37.8361371832878	32.62285295518794	15.291773910990678
6	34.764860401261416	33.9503078784254	16.306257476076556	14.978574244236626
7	30.263954777305415	31.95640817643986	19.075245161536927	18.704391884717804
8	28.53747884056937	34.71080908226888	18.21677279294292	18.534939284218822
9	28.413184789864438	15.021766861036895	18.196858030468512	38.368190318630155
10-11	27.067387496368383	25.159451290476586	26.010226290009363	21.762934923145664
12-13	26.69384371132304	22.353572274997262	26.87516086641483	24.077423147264867
14-15	24.170474401741746	23.590288555388213	24.44727316899548	27.791963873874565
16-17	24.27569500068377	26.86008081195888	24.95037377866833	23.913850408689026
18-19	24.181663764915708	25.889464520786444	27.045394708904837	22.88347700539301
20-21	24.761728049619837	24.90150463636221	27.058318511438795	23.278448802579156
22-23	24.53681692063697	24.378559518028098	26.954275157323483	24.13034840401145
24-25	24.075151882554064	24.741945056106655	26.971735050430823	24.21116801090846
26-27	23.826114199210576	24.970008125187316	27.10238160532306	24.101496070279048
28-29	23.9373157328341	25.650838528367327	26.755305939213326	23.656539799585246
30-31	24.707820117971437	24.969021354994684	26.80519855411764	23.517959972916238
32-33	24.19306838429427	24.936690643206084	26.868724519708582	24.001516452791066
34-35	24.137815390036145	25.281975195437283	26.976012633643442	23.604196780883125
36-37	24.219872999323307	25.187236423502	26.96892227725	23.623968299924687
38-39	24.308049548746425	25.33544739054023	26.82679164225737	23.529711418455975
40-41	24.181253271399783	25.040678914419544	26.92016574667723	23.857902067503435
42-43	24.051632940345417	25.184791486601448	26.618591730103397	24.144983842949735
44-45	23.86753953661932	25.20286754845154	26.855142275792886	24.074450639136256
46-47	23.858628490551702	25.08107245302595	26.566972702175956	24.4933263542464
48-49	23.952448125980883	25.496007698907185	26.347404272010504	24.204139903101428
50-51	24.081909361937143	25.568445928707543	26.280852595665706	24.06879211368961
52-53	24.181725253938552	25.543705278457928	26.157029284013845	24.11754018358968
54-55	23.796707334312845	25.1832336488511	26.484006827987276	24.53605218884878
56-57	23.940853073808853	25.36485017360072	26.200103324159812	24.494193428430613
58-59	23.95969085967872	25.52559882565393	26.358729104883743	24.155981209783615
60-61	24.330083775345386	25.326600741603894	26.530911128515665	23.812404354535055
62-63	24.267633405994435	25.32167925677669	26.374876097610674	24.035811239618198
64-65	23.912024675405167	25.124782124764295	26.491124104620866	24.472069095209672
66-67	23.96855880959537	25.34118174454797	26.258683429867773	24.431576015988888
68-69	23.75743729241804	25.42293233082707	26.39270941581596	24.426920960938933
70-71	23.788409928913033	25.29029513869727	26.496685070208255	24.42460986218144
72-73	23.915418889685082	25.73117636870579	26.002947798829712	24.350456942779424
74-75	23.845464490802986	25.62934045281862	26.12908552849145	24.39610952788694
76-77	23.753664568746164	25.174748882057134	26.158249938658535	24.913336610538167
78-79	23.63436847992427	25.205617851131613	26.453100027380337	24.706913641563776
80-81	23.85017485204011	25.55093886928165	25.963851176674847	24.635035102003393
82-83	23.957968952178476	25.57068456676708	25.987904526760154	24.483441954294292
84-85	23.919065029934174	25.61573803450315	25.83818015179552	24.627016783767157
86-87	23.625995773666155	25.783947572405047	25.95120104121652	24.63885561271228
88-89	23.488109787547273	25.362899922187975	26.140850379742215	25.008139910522537
90-91	23.758453991438607	25.17807930748162	26.174729124180917	24.888737576898848
92-93	23.757166641338095	25.394637531169	26.087805524118885	24.76039030337402
94-95	23.7086110707824	25.250398829703325	26.049204420705692	24.99178567880858
96-97	23.371937832069158	25.248208163743524	26.13631539714155	25.243538607045767
98-99	23.356651312373895	25.22837228789999	26.00933143665155	25.405644963074558
100-101	23.27939055213172	25.34429169004961	26.108096680994997	25.26822107682368
102-103	23.21450857968096	25.206585608753645	26.237709504084165	25.34119630748123
104-105	23.351046352982927	25.40440105853617	25.992207856158622	25.252344732322285
106-107	23.360579361929283	25.177028435344983	26.13131156622568	25.331080636500058
108-109	23.3319465964808	25.11633731666419	26.111009700082782	25.440706386772227
110-111	23.125508870801365	25.54083574096258	26.065179610698447	25.268475777537603
112-113	23.1093419750639	25.575317071224234	26.013742830690312	25.301598123021556
114-115	23.147115981366095	25.621830567991083	25.923905614707436	25.307147835935385
116-117	22.960362768204632	25.424126700582082	25.978959647895454	25.636550883317827
118-119	22.977975902161912	25.274075445090034	26.100557511375854	25.6473911413722
120-121	22.859801515168236	25.32808410092178	26.298518538014626	25.51359584589535
122-123	22.780634932089818	25.3972835926705	26.023258627532968	25.79882284770672
124-125	22.57444515645424	25.373436859934834	25.988026903771594	26.06409107983934
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	30.5
2	26.5
3	61.0
4	71.5
5	70.5
6	177.5
7	455.5
8	778.5
9	884.5
10	768.0
11	654.5
12	644.5
13	710.0
14	777.5
15	816.0
16	895.5
17	981.5
18	985.0
19	1031.5
20	1125.0
21	1162.0
22	1246.5
23	1436.0
24	1650.5
25	1975.0
26	2490.5
27	3087.5
28	3790.0
29	4832.0
30	5983.5
31	7295.0
32	9101.5
33	10871.5
34	12556.5
35	14308.0
36	16503.0
37	18706.0
38	20865.5
39	23549.5
40	25962.5
41	27982.5
42	30225.5
43	33392.5
44	36838.0
45	39971.5
46	43023.5
47	46169.5
48	49840.5
49	53163.0
50	55147.5
51	55877.5
52	55050.5
53	53513.0
54	52464.0
55	50587.0
56	47126.5
57	42946.0
58	38786.0
59	33991.5
60	28633.5
61	23925.0
62	19717.5
63	15110.0
64	11017.5
65	8374.5
66	6260.5
67	4694.5
68	3676.0
69	2820.5
70	2170.5
71	1610.0
72	1155.5
73	957.5
74	770.5
75	565.0
76	404.5
77	251.5
78	140.0
79	72.0
80	36.0
81	20.0
82	15.5
83	9.0
84	4.0
85	2.0
86	3.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022923064253349102
2	0.0016131045215319738
3	0.029799983529353834
4	0.16428196048233523
5	0.020885458541940294
6	0.0649486820511558
7	0.04822333517000848
8	0.04092191470412691
9	0.03183758924076264
10-11	0.05866606444097863
12-13	0.05382675087638271
14-15	0.05467575325613638
16-17	0.046864931362402606
18-19	0.03421479590407292
20-21	0.022031611754607747
22-23	0.002122505949384176
24-25	0.004372362255731403
26-27	0.0028866080911624796
28-29	0.0038629608278792004
30-31	0.0021649560683718594
32-33	0.008872074868425856
34-35	0.0038629608278792004
36-37	0.00543361523042349
38-39	0.001952705473433442
40-41	0.002674357496224062
42-43	0.0033111092810393147
44-45	0.00445726249370677
46-47	0.004117661541805302
48-49	0.001995155592421126
50-51	0.0012310534506428223
52-53	7.641021417783034E-4
54-55	0.004542162731682137
56-57	5.518515468398858E-4
58-59	5.094014278522023E-4
60-61	4.245011898768352E-5
62-63	8.91452498741354E-4
64-65	0.0
66-67	0.0029715083291378466
68-69	4.2450118987683526E-4
70-71	0.0
72-73	0.002122505949384176
74-75	0.00445726249370677
76-77	0.001698004759507341
78-79	4.245011898768352E-5
80-81	0.0
82-83	0.004839313564595922
84-85	0.0015706544025442905
86-87	0.0
88-89	0.0012310534506428223
90-91	5.943016658275693E-4
92-93	0.0
94-95	0.002716807615211745
96-97	9.339026177290375E-4
98-99	5.518515468398858E-4
100-101	0.0
102-103	0.0010188028557044045
104-105	0.0
106-107	4.6695130886451877E-4
108-109	0.002377206663310277
110-111	0.0
112-113	2.1225059493841763E-4
114-115	0.0
116-117	0.001782904997482708
118-119	0.002504557020273328
120-121	0.0025470071392610116
122-123	8.91452498741354E-4
124-125	0.10315378914007096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1177853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.74176622457847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.32259738236804	54.12032952111735
2	10.040932835238268	13.202173182618562
3	3.0927308964011173	6.099647747601998
4	1.445093865586854	3.8001209233593354
5	0.8041418190503982	2.6432851739709284
6	0.5213822296252408	2.0565953192203246
7	0.34573175117251725	1.5910311173398435
8	0.24733144128100853	1.3008004634147297
9	0.18281807642770015	1.0816904917923285
>10	0.9521203148221432	10.730836492240673
>50	0.03256013711281881	1.4538609371075162
>100	0.011782394060726915	1.2184959915321976
>500	3.884284265807043E-4	0.16751596854803677
>1k	3.884284265807043E-4	0.5336166701359971
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2618	0.2222688230195109	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.17863010070017227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1547	0.13134066814789283	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.490023797536705E-5	0.0
3	0.0	0.0	0.0	8.490023797536705E-5	0.0
4	0.0	0.0	0.0	8.490023797536705E-5	0.0
5	0.0	0.0	0.0	8.490023797536705E-5	0.0
6	0.0	0.0	0.0	8.490023797536705E-5	0.0
7	0.0	0.0	0.0	8.490023797536705E-5	0.0
8	0.0	0.0	0.0	8.490023797536705E-5	0.0
9	0.0	0.0	0.0	8.490023797536705E-5	0.0
10-11	0.0	0.0	0.0	1.698004759507341E-4	0.0
12-13	0.0	0.0	0.0	2.1225059493841763E-4	0.0
14-15	0.0	0.0	0.0	2.5470071392610114E-4	0.0
16-17	0.0	0.0	0.0	2.5470071392610114E-4	8.490023797536705E-5
18-19	0.0	0.0	0.0	2.9715083291378464E-4	1.698004759507341E-4
20-21	0.0	0.0	0.0	3.820510708891517E-4	2.1225059493841763E-4
22-23	0.0	0.0	0.0	5.518515468398858E-4	2.9715083291378464E-4
24-25	0.0	0.0	0.0	0.0012310534506428223	3.396009519014682E-4
26-27	0.0	0.0	0.0	0.0018253551164703914	3.396009519014682E-4
28-29	0.0	0.0	0.0	0.004627062969657504	3.396009519014682E-4
30-31	0.0	0.0	0.0	0.0149424418836646	3.396009519014682E-4
32-33	0.0	0.0	0.0	0.0371014039952354	3.396009519014682E-4
34-35	0.0	8.490023797536705E-5	0.0	0.06418457990937748	3.396009519014682E-4
36-37	0.0	8.490023797536705E-5	0.0	0.10052188176283458	3.396009519014682E-4
38-39	0.0	8.490023797536705E-5	0.0	0.1656828144089288	4.245011898768352E-4
40-41	0.0	8.490023797536705E-5	0.0	0.24587108917666295	4.245011898768352E-4
42-43	0.0	8.490023797536705E-5	0.0	0.3148525325316487	4.245011898768352E-4
44-45	0.0	8.490023797536705E-5	0.0	0.38701773481071067	4.245011898768352E-4
46-47	0.0	8.490023797536705E-5	0.0	0.4621544454189105	4.245011898768352E-4
48-49	0.0	8.490023797536705E-5	0.0	0.5439558247081766	4.245011898768352E-4
50-51	0.0	8.490023797536705E-5	0.0	0.6311908192278664	4.245011898768352E-4
52-53	0.0	8.490023797536705E-5	0.0	0.7242414800488686	4.6695130886451877E-4
54-55	0.0	8.490023797536705E-5	0.0	0.8263340162142474	6.367517848152528E-4
56-57	0.0	8.490023797536705E-5	0.0	0.9282567519036755	6.792019038029364E-4
58-59	0.0	8.490023797536705E-5	0.0	1.0421928712666182	6.792019038029364E-4
60-61	0.0	8.490023797536705E-5	0.0	1.1604589027663046	6.792019038029364E-4
62-63	0.0	8.490023797536705E-5	0.0	1.3012659474484507	6.792019038029364E-4
64-65	0.0	8.490023797536705E-5	0.0	1.43065391012291	6.792019038029364E-4
66-67	0.0	8.490023797536705E-5	0.0	1.5747296139671079	6.792019038029364E-4
68-69	0.0	8.490023797536705E-5	0.0	1.7372286694519605	6.792019038029364E-4
70-71	0.0	8.490023797536705E-5	0.0	1.9037180361216552	7.641021417783034E-4
72-73	0.0	8.490023797536705E-5	0.0	2.0673207947001875	0.0010188028557044045
74-75	0.0	8.490023797536705E-5	0.0	2.2379278229116877	0.0010188028557044045
76-77	0.0	8.490023797536705E-5	0.0	2.422967891579	0.0010188028557044045
78-79	0.0	1.2735035696305057E-4	0.0	2.6341572335427257	0.0010188028557044045
80-81	0.0	1.698004759507341E-4	0.0	2.8351160968304194	0.0010188028557044045
82-83	0.0	1.698004759507341E-4	0.0	3.049022246409357	0.0011037030936797716
84-85	0.0	1.698004759507341E-4	0.0	3.285809010122655	0.0011037030936797716
86-87	0.0	1.698004759507341E-4	0.0	3.540721974643695	0.0011037030936797716
88-89	0.0	1.698004759507341E-4	0.0	3.829212983283992	0.0011037030936797716
90-91	0.0	1.698004759507341E-4	0.0	4.132391733094028	0.0011037030936797716
92-93	0.0	1.698004759507341E-4	0.0	4.420373340306472	0.0011037030936797716
94-95	0.0	1.698004759507341E-4	0.0	4.718712776551913	0.0011037030936797716
96-97	0.0	1.698004759507341E-4	0.0	5.053177264055871	0.0011037030936797716
98-99	0.0	1.698004759507341E-4	0.0	5.425167656744942	0.0011037030936797716
100-101	0.0	1.698004759507341E-4	0.0	5.820463164758251	0.0011037030936797716
102-103	0.0	1.698004759507341E-4	0.0	6.229894562394458	0.001231053450642822
104-105	0.0	1.698004759507341E-4	0.0	6.653759000486478	0.0013584038076058728
106-107	0.0	2.5470071392610114E-4	0.0	7.103178410209084	0.0013584038076058728
108-109	0.0	2.5470071392610114E-4	0.0	7.575818035017952	0.0013584038076058728
110-111	0.0	2.5470071392610114E-4	0.0	8.094176437976556	0.0013584038076058728
112-113	0.0	2.5470071392610114E-4	0.0	8.593729438223615	0.0013584038076058728
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1885	0.0	56.636547	1
GTATCAA	3285	0.0	50.29207	1
TCAACGC	4140	0.0	39.319366	4
CAACGCA	4180	0.0	38.90172	5
GTACATG	6410	0.0	38.24185	1
ATCAACG	4300	0.0	37.85793	3
TATCAAC	4335	0.0	37.557068	2
AACGCAG	4330	0.0	37.429253	6
TACATGG	6660	0.0	36.355484	2
ACATGGG	6745	0.0	35.27391	3
CATGGGG	5760	0.0	35.196594	4
ATGGGGG	3380	0.0	32.60149	5
ACGCAGA	5410	0.0	29.845835	7
CGCAGAG	5760	0.0	28.0299	8
TGGGGGG	2525	0.0	27.609251	6
ATGGGGA	2050	0.0	26.149961	5
GCAGAGT	6280	0.0	25.424353	9
ATACCGA	150	1.6705577E-4	23.833628	6
GAGTACA	4460	0.0	23.803469	1
GAGTACT	3240	0.0	23.149923	12-13
>>END_MODULE