FastQCFastQC Report
Mon 15 Aug 2016
SRR2921624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431420
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89150.035.5939371
CATGGGG71650.035.2194944
TACATGG91800.034.0380942
ACATGGG93850.032.9129263
ATGGGGG44950.030.8481645
GTATCAA39450.028.3714141
GGTATCA30650.025.0570161
TCAACGC46150.023.3421214
CAACGCA46650.023.2178965
AACGCAG48300.022.5440086
TATCAAC49250.022.1153352
ATGGGGA26550.021.9666925
ATCAACG49550.021.8605563
CGTACAC3859.094947E-1221.6421663
TGGGGGG39150.021.1256566
ATGGGGC18200.020.9272275
TCTATAC3801.9281288E-1020.3607233
GTATAGG4203.45608E-1119.8448981
GTCTCGT5001.8189894E-1219.0344589
GTATATG4759.094947E-1218.800431
Produced by FastQC (version 0.11.5)