FastQCFastQC Report
Mon 15 Aug 2016
SRR2921624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431420
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30170.21076972516801498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23370.16326445068533343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.12113844993083792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48200.039.973141
GGTATCA32550.037.5677871
ATGGGGG42400.035.819395
GTACATG87900.035.084431
CATGGGG69200.034.2105674
TACATGG90300.033.9478342
ACATGGG92800.032.389493
TCAACGC62300.030.5336344
CAACGCA62850.030.2293835
TATCAAC65200.029.3625762
AACGCAG65050.029.2203066
ATCAACG65850.028.890593
TGGGGGG32900.026.985586
ACGCAGA77300.024.5125797
CGCAGAG79800.023.7446428
CTATAGC3302.910383E-1123.4936713
GTATAGG4600.023.341431
AGTACTT41050.022.91264312-13
AGAGTAC79600.021.5960410-11
GCAGAGT86700.021.51139
Produced by FastQC (version 0.11.5)