FastQCFastQC Report
Mon 15 Aug 2016
SRR2921623_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921623_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970717
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.039.684061
GGTATCA23500.036.7288631
GTACATG94250.033.7261851
TCAACGC40100.032.503484
TACATGG97550.032.397272
ACATGGG97650.032.058533
CAACGCA41300.031.2684785
TATCAAC43400.030.3070532
ATCAACG44350.029.79133
AACGCAG43800.029.7539756
CATGGGG88300.028.9826374
ATGGGGG48950.026.8680785
ACGCAGA54550.023.665067
CGCAGAG58100.022.114458
GAGTACA71050.021.8667281
AGTACAT68850.021.6111182
GTATAGG4500.021.1648311
GCTACAC14650.020.312463
AGAGTAC57850.020.0598775
GTATTAG5700.019.842031
Produced by FastQC (version 0.11.5)