##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921623_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1970717
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.800110315179705	31.0	31.0	33.0	26.0	34.0
2	29.974676221902993	31.0	31.0	34.0	26.0	34.0
3	30.083561972622146	31.0	31.0	34.0	26.0	34.0
4	33.658031569220746	37.0	35.0	37.0	29.0	37.0
5	33.694725320784265	37.0	35.0	37.0	29.0	37.0
6	33.958998679160935	37.0	35.0	37.0	30.0	37.0
7	33.97494414469455	37.0	35.0	37.0	30.0	37.0
8	33.931908538871895	37.0	35.0	37.0	30.0	37.0
9	35.630073724436336	39.0	37.0	39.0	30.0	39.0
10-11	35.66027187059329	39.0	37.0	39.0	30.0	39.0
12-13	35.605300507378786	39.0	37.0	39.0	30.0	39.0
14-15	36.91844897060308	40.5	38.0	41.0	30.0	41.0
16-17	36.87551231353868	41.0	38.0	41.0	30.0	41.0
18-19	36.824323330036734	40.0	38.0	41.0	30.0	41.0
20-21	36.878516296353055	40.5	38.0	41.0	30.0	41.0
22-23	36.81247079108771	40.0	38.0	41.0	30.0	41.0
24-25	36.80007301910929	40.5	38.0	41.0	30.0	41.0
26-27	36.75949184992061	40.0	38.0	41.0	30.0	41.0
28-29	36.709123633682566	40.0	38.0	41.0	30.0	41.0
30-31	36.618895305617194	40.0	38.0	41.0	30.0	41.0
32-33	36.53894293295283	40.0	38.0	41.0	29.5	41.0
34-35	36.41759116098354	40.0	37.5	41.0	29.0	41.0
36-37	36.317509312600436	40.0	37.0	41.0	28.0	41.0
38-39	36.21034882228143	40.0	37.0	41.0	28.0	41.0
40-41	36.10513812992936	40.0	37.0	41.0	27.5	41.0
42-43	35.868503189448305	40.0	36.5	41.0	26.5	41.0
44-45	35.5403033515213	40.0	35.5	41.0	26.0	41.0
46-47	35.29850480814851	39.5	35.0	41.0	25.5	41.0
48-49	35.11167762799022	39.0	35.0	41.0	24.5	41.0
50-51	34.915115412309326	39.0	35.0	41.0	24.0	41.0
52-53	34.70925201335351	39.0	35.0	40.5	24.0	41.0
54-55	34.46814281299649	38.5	34.0	40.0	23.0	41.0
56-57	34.21388560610174	38.0	34.0	40.0	23.0	41.0
58-59	34.212296336815484	38.0	34.0	40.0	23.0	41.0
60-61	33.99211885826327	37.5	34.0	40.0	22.5	41.0
62-63	33.68538100599934	37.0	34.0	40.0	22.0	41.0
64-65	33.37811466588049	36.5	34.0	39.5	21.5	41.0
66-67	33.05143331082037	36.0	33.5	39.0	20.5	41.0
68-69	32.71208981299699	35.5	33.0	39.0	20.0	40.5
70-71	32.339019250354056	35.0	33.0	38.0	18.5	40.0
72-73	31.99017616430974	35.0	33.0	37.0	18.0	39.0
74-75	31.628212980351822	35.0	32.5	37.0	15.0	39.0
76-77	31.33158236317036	35.0	32.0	36.0	13.5	39.0
78-79	31.06225348439172	35.0	32.0	36.0	11.5	37.0
80-81	30.807097366085543	35.0	32.0	35.0	9.0	37.0
82-83	30.58656620915129	35.0	32.0	35.0	7.0	36.5
84-85	30.37828643077621	35.0	32.0	35.0	7.0	36.0
86-87	30.213898291839975	35.0	31.0	35.0	5.5	36.0
88-89	30.05794413911282	35.0	31.0	35.0	2.0	35.5
90-91	29.879024233312037	35.0	31.0	35.0	2.0	35.0
92-93	29.808420488583597	34.0	31.0	35.0	2.0	35.0
94-95	29.68919078690649	34.0	31.0	35.0	2.0	35.0
96-97	29.602206963252463	34.0	31.0	35.0	2.0	35.0
98-99	29.512678126793446	34.0	31.0	35.0	2.0	35.0
100-101	29.40486127637809	34.0	31.0	35.0	2.0	35.0
102-103	28.53132793800429	33.5	29.0	34.5	2.0	35.0
104-105	29.44584686690174	34.0	31.0	35.0	2.0	35.0
106-107	29.720550185541608	35.0	31.5	35.0	2.0	35.0
108-109	29.72613241779515	35.0	32.0	35.0	2.0	35.0
110-111	29.66271768092527	35.0	32.0	35.0	2.0	35.0
112-113	29.58390727841694	35.0	32.0	35.0	2.0	35.0
114-115	29.469363434729594	35.0	31.0	35.0	2.0	35.0
116-117	29.35397091515423	35.0	31.0	35.0	2.0	35.0
118-119	29.0755118568521	35.0	31.0	35.0	2.0	35.0
120-121	28.96073561044026	35.0	31.0	35.0	2.0	35.0
122-123	28.734362671048153	35.0	31.0	35.0	2.0	35.0
124-125	27.99056510904407	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75207.0
3	12291.0
4	6282.0
5	5578.0
6	5945.0
7	6394.0
8	6613.0
9	6851.0
10	7503.0
11	7533.0
12	7457.0
13	7479.0
14	7506.0
15	6929.0
16	6009.0
17	5545.0
18	5258.0
19	4920.0
20	4722.0
21	5170.0
22	5944.0
23	7959.0
24	11230.0
25	14377.0
26	16283.0
27	18609.0
28	22106.0
29	26732.0
30	32344.0
31	40885.0
32	53231.0
33	74166.0
34	113592.0
35	192776.0
36	397644.0
37	598150.0
38	143429.0
39	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83359344983508	17.05090314987126	11.601855785320902	25.51364761497276
2	17.26950246635406	18.88273146174614	37.77160343462639	26.07616263727341
3	20.907365493722534	22.279679681711613	27.704943822505506	29.108011002060348
4	13.922024564099388	14.706691336998698	33.771120081115725	37.600164017786184
5	15.512820903203018	36.91367573158267	32.638112841000094	14.935390524214217
6	35.99842361650003	32.9282847078612	16.252747498303762	14.820544177335018
7	31.152997513283974	30.926664723866885	18.755057964142143	19.165279798706987
8	28.17968417643314	34.357717452333546	18.30392814826021	19.158670222973104
9	27.79717191814195	15.554957771642034	17.676069920415788	38.97180038980023
10-11	27.19958858652514	24.86244123435211	25.987609367373093	21.950360811749654
12-13	26.727130814385742	22.193918331532586	26.444001686899483	24.634949167182192
14-15	24.185395888906005	23.546300119454543	23.317466122188776	28.950837869450673
16-17	25.061672434604375	26.8070530931137	23.909565369227536	24.221709103054383
18-19	25.00770324510939	26.120447788242235	25.313269531343195	23.558579435305177
20-21	25.44578934572219	25.064220770222878	25.227684900238916	24.262304983816012
22-23	25.529628484685123	24.676766092406496	25.00178932829377	24.791816094614603
24-25	25.034705919675147	25.162667343442674	25.009123786561766	24.793502950320413
26-27	24.861857838834737	25.263758248552836	25.156620340922416	24.717763571690007
28-29	25.01290044360266	25.521506619520295	25.00937233408811	24.45622060278893
30-31	25.331987652353455	25.217738423573817	25.000913587791217	24.449360336281515
32-33	24.76840866258953	25.247782808920842	25.156115731968715	24.827692796520914
34-35	24.901454538624492	25.454502074467626	25.202814554828336	24.44122883207955
36-37	25.02158030232215	25.495763359107904	25.019956387744113	24.462699950825836
38-39	25.17728030792874	25.515701462600603	25.056970267864305	24.250047961606352
40-41	24.956071208932006	25.442834955921455	25.09130310193231	24.509790733214228
42-43	24.893709025888963	25.158821072334707	25.165368301968382	24.782101599807948
44-45	24.824641937639978	25.26886713692723	25.264451783067067	24.642039142365725
46-47	24.870367574193043	25.278151737123988	25.21328794221133	24.638192746471635
48-49	24.721450500377763	25.4708560694136	25.20955769362055	24.59813573658809
50-51	24.681595139511895	25.459344295994384	25.206625308190016	24.652435256303697
52-53	24.8110777538948	25.46759175842721	25.18729809085626	24.534032396821733
54-55	24.657403537407923	25.134060588711936	25.2757152583802	24.932820615499946
56-57	24.705802935740422	25.339052108237826	25.063678935842947	24.891466020178807
58-59	24.752764430152844	25.51205650866733	25.186790621948568	24.548388439231257
60-61	24.987133899813937	25.2298895494784	25.196265125915218	24.586711424792444
62-63	24.800708928833807	25.353722142834844	25.325046255728516	24.52052267260283
64-65	24.67303584234666	25.100884474271478	25.34716389140258	24.878915791979285
66-67	24.667531896686054	25.51338983214301	25.08030955405801	24.738768717112926
68-69	24.717142538843813	25.338217205600483	25.164258439537846	24.78038181601786
70-71	24.64851240749742	25.219436233979014	25.341956542983002	24.790094815540563
72-73	24.75411845403905	25.40906665123761	25.15288849436522	24.683926400358118
74-75	24.59427989136985	25.39436535019978	25.221087930488075	24.790266827942297
76-77	24.574759847666687	25.16009309709219	25.382025927519873	24.883121127721253
78-79	24.589361008297157	25.153095859540848	25.366038109328628	24.891505022833368
80-81	24.71290283370708	25.46862403219295	25.06136539364825	24.75710774045173
82-83	24.668660985868385	25.459113834041446	25.126721661016827	24.745503519073342
84-85	24.724605930135812	25.41308793300362	25.090123316761353	24.77218282009922
86-87	24.57326330656959	25.488361565061123	25.17070863400358	24.76766649436571
88-89	24.612232789495334	25.24571934081668	25.22336218615922	24.918685683528764
90-91	24.7285482852636	25.110641252337167	25.250287768761815	24.910522693637418
92-93	24.60801187071617	25.42361613822645	25.318925271882893	24.649446719174488
94-95	24.690776923455584	25.124549417077773	25.322823664820604	24.861849994646036
96-97	24.481290106811088	25.282557234000603	25.306232131225414	24.929920527962896
98-99	24.57521583648341	25.22971558249843	25.231237969967356	24.96383061105081
100-101	24.5243406843242	25.19738364539859	25.36241555562773	24.915860114649472
102-103	24.302475986337495	25.21273448493364	25.502410627158874	24.98237890156999
104-105	24.310128767844144	25.295088218824397	25.482743038490806	24.912039974840656
106-107	24.239648734130647	25.249999873141586	25.48783402454254	25.02251736818523
108-109	24.251276229374668	25.243230871276808	25.57965097365936	24.92584192568917
110-111	24.182089337146547	25.500890042685516	25.40112813164107	24.91589248852687
112-113	24.091377781025393	25.607253551573166	25.47148795641699	24.829880710984455
114-115	24.233178649582435	25.60663130458083	25.360598881892255	24.799591163944477
116-117	23.98602060423284	25.376399087695738	25.60792585257035	25.029654455501067
118-119	24.18130694749954	25.33024270631869	25.526701562155747	24.961748784026028
120-121	24.10206239266168	25.520215822172513	25.560658376365918	24.817063408799882
122-123	24.04597961282276	25.628793227988332	25.47950613475063	24.845721024438276
124-125	24.013991381618393	25.476474546455886	25.593646259514475	24.915887812411246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	518.0
1	433.0
2	293.5
3	215.0
4	165.0
5	147.0
6	165.0
7	186.5
8	222.0
9	232.0
10	224.0
11	238.0
12	247.0
13	258.0
14	308.0
15	344.0
16	363.0
17	411.0
18	476.5
19	541.0
20	587.5
21	624.0
22	751.0
23	981.0
24	1205.0
25	1499.5
26	1914.0
27	2555.0
28	3460.5
29	4634.5
30	6259.0
31	8161.0
32	10567.5
33	13049.0
34	15758.5
35	19063.0
36	22855.5
37	26997.0
38	31656.5
39	36747.0
40	41325.0
41	45175.5
42	49695.5
43	56995.0
44	64678.5
45	71791.0
46	76199.0
47	80183.0
48	86816.5
49	90638.5
50	91287.5
51	90599.0
52	90208.5
53	89813.0
54	89847.5
55	88186.0
56	82027.5
57	74930.0
58	69454.5
59	64619.5
60	55937.0
61	45927.5
62	37626.0
63	28980.0
64	21634.5
65	16583.5
66	12690.5
67	9719.5
68	7556.0
69	5828.5
70	4582.5
71	3576.5
72	2720.5
73	2109.5
74	1613.5
75	1172.5
76	857.0
77	563.5
78	367.5
79	257.5
80	177.5
81	129.5
82	102.0
83	86.0
84	70.5
85	55.0
86	45.0
87	37.0
88	30.0
89	27.5
90	20.5
91	14.5
92	14.5
93	13.0
94	13.5
95	15.5
96	11.5
97	7.0
98	6.5
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022631357013716328
2	0.009996361730273804
3	0.008981502671362758
4	0.010097847636164909
5	0.013345396624680254
6	0.08387810121899797
7	0.053330843545775476
8	0.044806027450922686
9	0.02420438855502845
10-11	0.046810374092272004
12-13	0.012076822801041448
14-15	0.025955020431650003
16-17	0.032018803308643505
18-19	0.03897058786218417
20-21	0.02841605364950929
22-23	0.03572303887366882
24-25	0.03003982814376696
26-27	0.0419136791330262
28-29	0.041786821750662326
30-31	0.02349398721379072
32-33	0.02760416640238045
34-35	0.041304763697679575
36-37	0.008626302000743892
38-39	0.019840494601710952
40-41	0.0389452163857114
42-43	0.021210554331240864
44-45	0.016237744942576737
46-47	0.022047813054842477
48-49	0.028187710361254305
50-51	0.02762953787885323
52-53	0.023443244260845163
54-55	0.02194632714895137
56-57	0.024432731843283432
58-59	0.01669443151908671
60-61	0.021464269095968623
62-63	0.021337411713604745
64-65	0.019992723460547607
66-67	0.026792279155251616
68-69	0.02143889761949585
70-71	0.04259870899779116
72-73	0.020728496278258117
74-75	0.009006874147835534
76-77	0.015273628836611243
78-79	0.023570101643209045
80-81	0.017633176148579427
82-83	0.023037300637280746
84-85	0.011290307030385386
86-87	0.01661831708966838
88-89	0.021844841243060267
90-91	0.019536036884037638
92-93	0.007789043277142279
94-95	0.010858991930348193
96-97	0.01397968353649966
98-99	0.006495097977030695
100-101	0.009590418106709384
102-103	0.0034505208002975564
104-105	0.003932578853280304
106-107	8.118872471288369E-4
108-109	0.013396139577625808
110-111	0.0012685738236388076
112-113	0.0020804610707676444
114-115	0.012432023471660314
116-117	0.005201152676919112
118-119	0.2750268049648935
120-121	0.0010402305353838222
122-123	8.880016765471653E-4
124-125	0.30846641095601246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1970717.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.87661584013989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.80718608705818	47.7858422261734
2	10.903867125417358	13.057733260810911
3	3.6330260634788254	6.525999178204119
4	1.72909402798203	4.141291954598405
5	0.9862817089738065	2.9527605499190632
6	0.6436545863440741	2.3123915040160967
7	0.42836351742462037	1.7954270440935547
8	0.32471598841248056	1.5554315596260333
9	0.2376036350828453	1.280421142206868
>10	1.219358643983749	12.726795037881452
>50	0.05946523288504183	2.4108087927162942
>100	0.02611223037110145	2.854165651608002
>500	0.0011016655743998295	0.4905184997700255
>1k	1.6948701144612566E-4	0.11041359837587329
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.0742952945552305E-5	0.0
8	0.0	0.0	0.0	5.0742952945552305E-5	0.0
9	0.0	0.0	0.0	1.0148590589110461E-4	1.0148590589110461E-4
10-11	0.0	0.0	0.0	1.0148590589110461E-4	1.0148590589110461E-4
12-13	0.0	0.0	0.0	1.0148590589110461E-4	2.0297181178220922E-4
14-15	0.0	0.0	0.0	1.0148590589110461E-4	2.790862412005377E-4
16-17	0.0	0.0	0.0	1.2685738236388076E-4	4.566865765099707E-4
18-19	0.0	0.0	0.0	1.5222885883665692E-4	5.07429529455523E-4
20-21	0.0	0.0	0.0	2.0297181178220922E-4	5.328010059282991E-4
22-23	0.0	0.0	0.0	3.0445771767331385E-4	5.835439588738516E-4
24-25	0.0	0.0	0.0	6.596583882921799E-4	6.596583882921799E-4
26-27	0.0	0.0	0.0	0.0010909734883293746	7.104013412377322E-4
28-29	0.0	0.0	0.0	0.003678864088552542	7.104013412377322E-4
30-31	0.0	0.0	0.0	0.012457394948133091	7.357728177105085E-4
32-33	0.0	0.0	0.0	0.029050340561328693	7.611442941832846E-4
34-35	0.0	0.0	0.0	0.049753465363114036	8.118872471288369E-4
36-37	0.0	0.0	0.0	0.08222895524826751	8.118872471288369E-4
38-39	0.0	0.0	0.0	0.13758951691186508	8.626302000743892E-4
40-41	0.0	0.0	0.0	0.20180472386446152	8.626302000743892E-4
42-43	0.0	0.0	0.0	0.2596009472694456	8.626302000743892E-4
44-45	0.0	0.0	0.0	0.3235370679808415	8.880016765471653E-4
46-47	0.0	5.0742952945552305E-5	0.0	0.39873812424615	9.133731530199414E-4
48-49	0.0	1.0148590589110461E-4	0.0	0.4768822717823006	9.641161059654938E-4
50-51	0.0	1.0148590589110461E-4	0.0	0.5544428753595773	0.001014859058911046
52-53	5.0742952945552305E-5	1.0148590589110461E-4	0.0	0.630633419207324	0.0012432023471660313
54-55	5.0742952945552305E-5	1.0148590589110461E-4	0.0	0.7186724425678572	0.0013700597295299123
56-57	5.0742952945552305E-5	1.0148590589110461E-4	0.0	0.8104410729698887	0.0013954312060026884
58-59	5.0742952945552305E-5	1.0148590589110461E-4	0.0	0.9135507533552509	0.0014461741589482406
60-61	5.0742952945552305E-5	2.0297181178220922E-4	0.0	1.0187916377643265	0.001496917111893793
62-63	5.0742952945552305E-5	2.0297181178220922E-4	0.0	1.1307305919622146	0.0015222885883665691
64-65	5.0742952945552305E-5	2.0297181178220922E-4	0.0	1.242111373677702	0.0015222885883665691
66-67	5.0742952945552305E-5	2.0297181178220922E-4	0.0	1.3713790463064965	0.0015222885883665691
68-69	5.0742952945552305E-5	2.0297181178220922E-4	0.0	1.5170113212602316	0.0015222885883665691
70-71	5.0742952945552305E-5	2.2834328825498536E-4	0.0	1.6715489844559113	0.0015476600648393452
72-73	5.0742952945552305E-5	2.537147647277615E-4	0.0	1.8188811483333223	0.002029718117822092
74-75	5.0742952945552305E-5	2.537147647277615E-4	0.0	1.9695369756286671	0.0020804610707676444
76-77	5.0742952945552305E-5	2.537147647277615E-4	0.0	2.1433569609436565	0.0020804610707676444
78-79	5.0742952945552305E-5	2.537147647277615E-4	0.0	2.3393008737429066	0.0020804610707676444
80-81	5.0742952945552305E-5	2.537147647277615E-4	0.0	2.5388221647248184	0.0021058325472404207
82-83	5.0742952945552305E-5	2.537147647277615E-4	0.0	2.7443311241543054	0.0022326899296043015
84-85	5.0742952945552305E-5	2.537147647277615E-4	0.0	2.9622721070554525	0.0022326899296043015
86-87	5.0742952945552305E-5	2.537147647277615E-4	0.0	3.197237350669832	0.0022834328825498537
88-89	5.0742952945552305E-5	2.537147647277615E-4	0.0	3.4675704324872623	0.002334175835495406
90-91	5.0742952945552305E-5	2.537147647277615E-4	0.0	3.751274282405845	0.002334175835495406
92-93	5.0742952945552305E-5	2.537147647277615E-4	0.0	4.036855621583413	0.002537147647277615
94-95	5.0742952945552305E-5	3.0445771767331385E-4	0.0	4.336746473491628	0.0026386335531687196
96-97	5.0742952945552305E-5	3.0445771767331385E-4	0.0	4.657518050536937	0.0026893765061142723
98-99	5.0742952945552305E-5	3.0445771767331385E-4	0.0	5.013530608402931	0.0027401194590598245
100-101	5.0742952945552305E-5	3.0445771767331385E-4	0.0	5.408006324601655	0.0027401194590598245
102-103	5.0742952945552305E-5	3.0445771767331385E-4	0.0	5.814178291454328	0.0027908624120053767
104-105	5.0742952945552305E-5	3.0445771767331385E-4	0.0	6.228748217019491	0.0028669768414237053
106-107	5.0742952945552305E-5	3.552006706188661E-4	0.0	6.667116587516118	0.002943091270842034
108-109	5.0742952945552305E-5	3.552006706188661E-4	0.0	7.13524569991531	0.003069948653205914
110-111	5.0742952945552305E-5	3.552006706188661E-4	0.0	7.626209141140001	0.0032221775120425712
112-113	5.0742952945552305E-5	3.552006706188661E-4	0.0	8.139347252801898	0.0032982919414608998
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3405	0.0	39.68406	1
GGTATCA	2350	0.0	36.728863	1
GTACATG	9425	0.0	33.726185	1
TCAACGC	4010	0.0	32.50348	4
TACATGG	9755	0.0	32.39727	2
ACATGGG	9765	0.0	32.05853	3
CAACGCA	4130	0.0	31.268478	5
TATCAAC	4340	0.0	30.307053	2
ATCAACG	4435	0.0	29.7913	3
AACGCAG	4380	0.0	29.753975	6
CATGGGG	8830	0.0	28.982637	4
ATGGGGG	4895	0.0	26.868078	5
ACGCAGA	5455	0.0	23.66506	7
CGCAGAG	5810	0.0	22.11445	8
GAGTACA	7105	0.0	21.866728	1
AGTACAT	6885	0.0	21.611118	2
GTATAGG	450	0.0	21.164831	1
GCTACAC	1465	0.0	20.31246	3
AGAGTAC	5785	0.0	20.059877	5
GTATTAG	570	0.0	19.84203	1
>>END_MODULE