FastQCFastQC Report
Mon 15 Aug 2016
SRR2921623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1970717
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.12158011525754332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27450.044.3328441
GTATCAA42150.041.042911
GTACATG92500.034.502441
TACATGG95900.033.336322
ACATGGG97300.032.488863
TCAACGC52400.032.207754
CAACGCA54200.031.1009335
CATGGGG86350.030.7328514
TATCAAC55400.030.3606322
TATACCG2750.030.3236585
AACGCAG56250.029.981956
ATCAACG56250.029.901853
ATGGGGG47300.029.71925
ACGCAGA67950.024.7286517
CGCAGAG72200.023.2706538
GTATAGG4450.022.7890741
ATAACGC2153.3529996E-622.1933573
GAGTACA68800.022.1100391
AGTACAT67000.021.3652462
TGGGGGG31800.021.1761656
Produced by FastQC (version 0.11.5)