FastQCFastQC Report
Mon 15 Aug 2016
SRR2921622_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921622_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730654
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35200.040.0689471
GTACATG94600.038.05981
TACATGG96350.036.8062362
CATGGGG82950.035.005024
ACATGGG100000.034.9867973
ATGGGGG50900.033.1963045
TCAACGC40500.032.7624784
CAACGCA41750.031.7788095
GGTATCA24000.030.9956681
AACGCAG43300.030.6394586
TATCAAC44300.030.3550932
ATCAACG45000.029.6184523
GTATAAC4750.026.3104271
ACGCAGA52450.025.176557
CGCAGAG56650.023.4142998
TGGGGGG40100.022.9959536
GTATAGG4000.022.316881
GCAGAGT60750.021.0507899
AGTACAT65350.020.7594642
ATGGGGA33200.020.6086245
Produced by FastQC (version 0.11.5)