##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921622_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730654
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.894590715417408	31.0	31.0	34.0	26.0	34.0
2	29.969847814756733	31.0	31.0	34.0	26.0	34.0
3	30.158196843505404	31.0	31.0	34.0	26.0	34.0
4	33.6774352354659	37.0	35.0	37.0	28.0	37.0
5	33.64037987951376	37.0	35.0	37.0	28.0	37.0
6	33.61591745085962	37.0	35.0	37.0	28.0	37.0
7	33.43488184235555	37.0	35.0	37.0	28.0	37.0
8	33.48359059638726	37.0	35.0	37.0	28.0	37.0
9	35.086695549774824	39.0	37.0	39.0	28.0	39.0
10-11	35.148534022398465	39.0	37.0	39.0	28.0	39.0
12-13	35.107092174403434	39.0	37.0	39.0	27.0	39.0
14-15	36.375720970222815	40.0	38.0	41.0	27.0	41.0
16-17	36.32404541866832	40.0	38.0	41.0	27.0	41.0
18-19	36.26053330128379	40.0	38.0	41.0	27.0	41.0
20-21	36.326479758519035	40.0	38.0	41.0	27.0	41.0
22-23	36.2415771725602	40.0	38.0	41.0	27.0	41.0
24-25	36.2214726340447	40.0	37.5	41.0	27.0	41.0
26-27	36.1766251370869	40.0	37.0	41.0	27.0	41.0
28-29	36.12146939827372	40.0	37.0	41.0	27.0	41.0
30-31	36.02843000391759	40.0	37.0	41.0	27.0	41.0
32-33	35.94100149423282	40.0	37.0	41.0	26.5	41.0
34-35	35.82003364046193	40.0	37.0	41.0	25.5	41.0
36-37	35.72076769822275	40.0	37.0	41.0	25.0	41.0
38-39	35.604487667667826	40.0	36.0	41.0	25.0	41.0
40-41	35.49213303179029	40.0	36.0	41.0	24.0	41.0
42-43	35.24683501150432	40.0	35.5	41.0	23.5	41.0
44-45	34.89054166806306	39.5	35.0	41.0	22.5	41.0
46-47	34.639243314954925	39.0	35.0	41.0	21.5	41.0
48-49	34.44544490117609	39.0	35.0	41.0	20.5	41.0
50-51	34.242339023282526	39.0	34.0	40.5	19.0	41.0
52-53	34.04209795834407	38.5	34.0	40.0	18.0	41.0
54-55	33.80241284508631	38.0	34.0	40.0	16.0	41.0
56-57	33.546414534621015	38.0	33.5	40.0	14.5	41.0
58-59	33.553912567156694	38.0	34.0	40.0	14.5	41.0
60-61	33.340636545490895	37.0	33.5	40.0	13.0	41.0
62-63	33.02932879709058	37.0	33.0	40.0	10.5	41.0
64-65	32.73761624218359	36.0	33.0	39.0	8.0	41.0
66-67	32.420506640842135	36.0	33.0	39.0	7.5	41.0
68-69	32.085864938919045	35.0	32.5	39.0	6.5	40.5
70-71	31.723269642574426	35.0	32.0	37.5	2.0	40.0
72-73	31.3742634287385	35.0	32.0	37.0	2.0	39.0
74-75	31.009110717682447	35.0	32.0	36.5	2.0	39.0
76-77	30.714980001779672	35.0	31.0	36.0	2.0	38.5
78-79	30.449279289794497	35.0	31.0	36.0	2.0	37.0
80-81	30.198046807738578	35.0	31.0	35.0	2.0	37.0
82-83	29.97999917950093	35.0	31.0	35.0	2.0	36.5
84-85	29.775007020467406	35.0	31.0	35.0	2.0	36.0
86-87	29.60622978365404	34.5	31.0	35.0	2.0	36.0
88-89	29.450672115859092	34.0	31.0	35.0	2.0	35.5
90-91	29.269886701790192	34.0	30.0	35.0	2.0	35.0
92-93	29.193942290024467	34.0	30.0	35.0	2.0	35.0
94-95	29.0732289643106	34.0	30.0	35.0	2.0	35.0
96-97	28.984046782314664	34.0	30.0	35.0	2.0	35.0
98-99	28.892062480426475	34.0	30.0	35.0	2.0	35.0
100-101	28.786465694471566	34.0	29.5	35.0	2.0	35.0
102-103	27.901431481971557	33.0	28.0	34.5	2.0	35.0
104-105	28.821163849042037	34.0	30.0	35.0	2.0	35.0
106-107	29.096394195489104	34.5	31.0	35.0	2.0	35.0
108-109	29.09595158824352	35.0	31.0	35.0	2.0	35.0
110-111	29.036590213872906	35.0	31.0	35.0	2.0	35.0
112-113	28.948919021364176	35.0	31.0	35.0	2.0	35.0
114-115	28.82879477931464	34.5	30.5	35.0	2.0	35.0
116-117	28.708862661167394	34.0	30.0	35.0	2.0	35.0
118-119	28.42414630538513	34.0	30.0	35.0	2.0	35.0
120-121	28.289191831527273	34.0	30.0	35.0	2.0	35.0
122-123	28.04607188958625	34.0	29.5	35.0	2.0	35.0
124-125	27.2829748176123	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	80716.0
3	13816.0
4	6570.0
5	5576.0
6	5823.0
7	6232.0
8	6534.0
9	6629.0
10	6988.0
11	7163.0
12	7121.0
13	7075.0
14	7079.0
15	6312.0
16	5675.0
17	5232.0
18	4844.0
19	4766.0
20	4601.0
21	5058.0
22	5657.0
23	7444.0
24	10171.0
25	13535.0
26	14943.0
27	17462.0
28	20828.0
29	25017.0
30	30233.0
31	38394.0
32	50032.0
33	69030.0
34	106254.0
35	172980.0
36	335348.0
37	490521.0
38	118940.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.171984855428995	16.835244706422227	11.623706767260586	25.3690636708882
2	16.576566060262135	18.81687839105262	38.94547906843967	25.661076480245576
3	20.169141866512568	23.336839063854377	28.210517191563135	28.283501878069924
4	13.714282742336456	14.516321037223499	34.20096089723808	37.56843532320196
5	14.708443833662644	37.80021463406741	32.332478225969105	15.158863306300846
6	35.41343530274482	32.99640400521414	16.877522198819534	14.712638493221508
7	31.553847372367557	30.872982974632208	19.229721924966743	18.343447728033492
8	27.803377018722227	35.5809023689077	18.4158453617008	18.19987525066927
9	28.559852781046484	15.981220798376153	17.81845230526664	37.640474115310724
10-11	27.255147878573105	25.059854683840378	26.077044677333898	21.607952760252616
12-13	26.787197192841326	22.28066616852545	26.56332259035744	24.368814048275777
14-15	24.396713655725673	23.683670579531725	23.61312919390356	28.30648657083904
16-17	25.151408133209102	26.73057270942817	24.097591341963316	24.02042781539941
18-19	25.062320462875213	26.163727163976013	25.38952276326259	23.384429609886187
20-21	25.658054632630055	25.249507775741677	25.007593243732572	24.084844347895697
22-23	25.588107872834886	24.76592013322401	24.98250741249299	24.663464581448117
24-25	25.10541920153932	25.062965413022763	24.966382321652905	24.865233063785013
26-27	24.766296118274123	25.27426949382151	25.129522372072362	24.829912015832008
28-29	24.96487514744345	25.71908037485393	24.901374385405404	24.414670092297218
30-31	25.62888406293392	25.250003251017567	24.943598457428287	24.177514228620225
32-33	25.05219194614115	25.259428073371588	25.05612223665897	24.632257743828287
34-35	24.971039056231632	25.636996247216626	25.087056250130065	24.304908446421674
36-37	25.129861987147002	25.539081104726925	24.980829140400036	24.350227767726036
38-39	25.20783908039334	25.53081682131185	24.93126934268814	24.33007475560667
40-41	25.080074059473016	25.364820363002405	25.02261611677951	24.53248946074507
42-43	25.032458666312195	25.356799704093707	24.920597996564133	24.69014363302996
44-45	24.670187669977313	25.360239207849382	25.332268440768434	24.637304681404874
46-47	24.670251488506338	25.25499087419333	25.268514868880825	24.806242768419505
48-49	24.695635085772413	25.463829934803716	25.132733388819535	24.707801590604337
50-51	24.755630384598934	25.489432805869495	25.238214136678216	24.516722672853355
52-53	24.83836709806341	25.48804857150701	25.066977992842006	24.60660633758758
54-55	24.629896876829566	25.066940767895712	25.41680128026928	24.886361075005446
56-57	24.695523615577052	25.283250815139883	25.096743226095803	24.924482343187258
58-59	24.78518204732482	25.423748403873063	25.20338969827386	24.587679850528257
60-61	25.123095199496724	25.226634163003215	25.266656899385154	24.38361373811491
62-63	24.883030956809808	25.354605983879352	25.25439014995098	24.507972909359864
64-65	24.703533555222023	25.083757297157653	25.296061889953	24.916647257667325
66-67	24.67140417243442	25.33402883540009	25.159045524559797	24.83552146760569
68-69	24.67733434126618	25.29501107917012	25.28726664100599	24.74038793855771
70-71	24.657196906744204	25.152372184976812	25.41296641265281	24.777464495626173
72-73	24.716996180380367	25.465113389359047	25.07930811078226	24.738582319478326
74-75	24.6701666286236	25.418709675555263	25.168521145683748	24.742602550137388
76-77	24.599980755733334	25.02907589089252	25.31973366628419	25.05120968708996
78-79	24.569819373985865	25.02764801840215	25.509201798024257	24.89333080958773
80-81	24.66578148545736	25.379108471299755	25.080294797128317	24.874815246114572
82-83	24.789126135933127	25.37072158465832	25.1610686491271	24.679083630281458
84-85	24.755185592595193	25.47332519091204	24.975504834454288	24.795984382038476
86-87	24.532709875291054	25.584463018732055	25.18202975237943	24.700797353597455
88-89	24.497875915064345	25.20305304158386	25.27541150262574	25.02365954072605
90-91	24.802959729086425	25.021246395481462	25.26597366284968	24.90982021258243
92-93	24.665802381993217	25.33964394052558	25.292750670244384	24.70180300723682
94-95	24.691354100812386	25.141205065468064	25.22262840790494	24.944812425814607
96-97	24.46256366335701	25.23137834165804	25.28711718912084	25.01894080586411
98-99	24.458565895213237	25.20714596516816	25.193595305057432	25.14069283456117
100-101	24.414570284215774	25.22911259932932	25.353760023947096	25.002557092507804
102-103	24.272727404053814	25.094700225500805	25.52706659713364	25.10550577331174
104-105	24.37159941383949	25.277334125358834	25.383281019586452	24.96778544121522
106-107	24.320280358711226	25.14832663061064	25.498688346507652	25.03270466417048
108-109	24.312054909625306	25.103660583897312	25.61258709656168	24.971697409915702
110-111	24.122567905086726	25.458177862717825	25.481290799750962	24.937963432444494
112-113	24.152423623795297	25.49561975510383	25.477302524946392	24.87465409615448
114-115	24.26314429906607	25.569960477962955	25.33637651284914	24.830518710121837
116-117	24.04631331871957	25.445917666559474	25.441814949549585	25.06595406517137
118-119	24.161130018075166	25.261806004408843	25.57443483596619	25.002629141549793
120-121	24.067126825450437	25.440727085932934	25.616760789178766	24.875385299437866
122-123	24.020107064716978	25.493207552948032	25.506381904219577	24.98030347811541
124-125	23.98501206633572	25.43279846538583	25.568111835094477	25.014077633183973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	350.0
1	303.5
2	228.5
3	186.0
4	163.0
5	142.0
6	146.5
7	163.5
8	174.5
9	192.5
10	204.5
11	224.0
12	231.5
13	246.5
14	283.0
15	305.5
16	348.0
17	386.5
18	424.5
19	465.0
20	518.5
21	586.5
22	672.5
23	821.5
24	1015.0
25	1284.0
26	1643.0
27	2166.0
28	2903.0
29	3967.0
30	5468.0
31	7323.0
32	9598.0
33	12068.0
34	14772.0
35	17808.5
36	21424.0
37	25246.0
38	28951.0
39	32829.0
40	36677.5
41	40252.0
42	44029.0
43	49659.5
44	55706.5
45	61108.0
46	65231.5
47	68862.5
48	74638.5
49	79367.5
50	81961.0
51	82937.0
52	81367.5
53	79904.0
54	79327.0
55	76233.5
56	71076.0
57	65264.0
58	59840.0
59	54294.0
60	46477.5
61	39168.0
62	32664.5
63	25387.5
64	19029.5
65	14542.5
66	11193.0
67	8829.0
68	6819.5
69	5156.5
70	4132.5
71	3207.5
72	2470.0
73	2008.5
74	1552.5
75	1182.0
76	873.5
77	548.0
78	354.0
79	253.5
80	201.5
81	150.5
82	121.0
83	92.5
84	64.0
85	57.0
86	47.5
87	42.0
88	40.0
89	36.0
90	30.0
91	21.5
92	17.0
93	15.5
94	18.5
95	13.0
96	9.5
97	10.5
98	6.5
99	6.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022939305025730158
2	0.010458474079740954
3	0.008898370211492304
4	0.010862945452990602
5	0.015427693808236654
6	0.08713469012292463
7	0.05437250888970297
8	0.045358575428710766
9	0.025539478139477913
10-11	0.04850767397758304
12-13	0.012769739069738956
14-15	0.026810673883976805
16-17	0.03247327310947191
18-19	0.039551522141340785
20-21	0.028746358313100136
22-23	0.03672022252859324
24-25	0.031057623303098136
26-27	0.04278729312733799
28-29	0.04353845424908734
30-31	0.024643862955853683
32-33	0.029006375624474907
34-35	0.042642839065463116
36-37	0.009418404834241853
38-39	0.020165787037732558
40-41	0.040042665951715364
42-43	0.02175478171835618
44-45	0.015947728430986206
46-47	0.022881523400980208
48-49	0.028544122626475307
50-51	0.028197432877975607
52-53	0.024354954832103932
54-55	0.022708178526730356
56-57	0.024817207830103532
58-59	0.01695890686411033
60-61	0.022390379590605632
62-63	0.022448161215355582
64-65	0.020628040035732157
66-67	0.02825521450272556
68-69	0.021957017404981006
70-71	0.04400070724708694
72-73	0.021783672530731157
74-75	0.009187278335242053
76-77	0.015514366245361582
78-79	0.024354954832103932
80-81	0.01799897610960943
82-83	0.023892701834104333
84-85	0.012163032009864478
86-87	0.017623395548734756
88-89	0.021928126592606033
90-91	0.020830275722356982
92-93	0.007424938780368577
94-95	0.011094071951990404
96-97	0.015196567309236854
98-99	0.006413760347244453
100-101	0.009996221081741353
102-103	0.003553569922121926
104-105	0.004044713732496501
106-107	8.089427464993002E-4
108-109	0.014272061313237654
110-111	0.0012423049321239254
112-113	0.002484609864247851
114-115	0.013116428818238653
116-117	0.005402581914120326
118-119	0.2776695977358848
120-121	0.0011267416826240253
122-123	0.0010689600578740754
124-125	0.31199188283735513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1730654.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.839365319952876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.45217775661784	52.63694697230419
2	9.545427088532842	12.187480140796444
3	3.1373046859131017	6.008506198920196
4	1.439487226866434	3.675838037973287
5	0.8431676667387928	2.6913644351455037
6	0.5674990481389445	2.173726743172056
7	0.3816302430884051	1.705412275396418
8	0.2782078141738057	1.4208488227125715
9	0.21040681940805256	1.2089014029000016
>10	1.0748437653285676	11.8888019039654
>50	0.05103073380765552	2.1878281397256116
>100	0.018364928472102665	1.9894505576502186
>500	3.617783309354345E-4	0.1561269254659869
>1k	9.044458273385861E-5	0.06876744387203784
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1556324949990003E-4
2	0.0	0.0	0.0	0.0	1.1556324949990003E-4
3	0.0	0.0	0.0	0.0	1.1556324949990003E-4
4	0.0	0.0	0.0	0.0	1.1556324949990003E-4
5	0.0	0.0	0.0	0.0	1.1556324949990003E-4
6	0.0	0.0	0.0	0.0	1.1556324949990003E-4
7	0.0	0.0	0.0	0.0	1.1556324949990003E-4
8	0.0	0.0	0.0	0.0	1.7334487424985006E-4
9	0.0	0.0	0.0	0.0	1.7334487424985006E-4
10-11	0.0	0.0	0.0	5.778162474995002E-5	1.7334487424985006E-4
12-13	0.0	0.0	0.0	5.778162474995002E-5	3.177989361247251E-4
14-15	0.0	0.0	0.0	5.778162474995002E-5	3.7558056087467513E-4
16-17	0.0	0.0	0.0	5.778162474995002E-5	4.6225299799960013E-4
18-19	0.0	0.0	0.0	8.667243712492503E-5	5.200346227495501E-4
20-21	0.0	0.0	0.0	1.1556324949990003E-4	7.222703093743752E-4
22-23	0.0	0.0	0.0	2.6001731137477506E-4	8.956151836242253E-4
24-25	0.0	0.0	0.0	4.333621856246251E-4	9.822876207491503E-4
26-27	0.0	0.0	0.0	7.511611217493502E-4	9.822876207491503E-4
28-29	0.0	0.0	0.0	0.0021957017404981008	9.822876207491503E-4
30-31	0.0	0.0	0.0	0.008840588586742353	9.822876207491503E-4
32-33	0.0	0.0	0.0	0.02519278839097821	9.822876207491503E-4
34-35	0.0	0.0	0.0	0.047900966917708565	9.822876207491503E-4
36-37	0.0	0.0	0.0	0.07505833055018507	9.822876207491503E-4
38-39	0.0	0.0	0.0	0.1239126942762678	9.822876207491503E-4
40-41	0.0	0.0	0.0	0.19013043623971054	0.0010978508702490504
42-43	0.0	0.0	0.0	0.24698755499366137	0.0011556324949990003
44-45	0.0	0.0	0.0	0.30344020237436253	0.0011556324949990003
46-47	0.0	0.0	0.0	0.36442870729793475	0.0011556324949990003
48-49	0.0	0.0	0.0	0.43032865032525275	0.0011845233073739755
50-51	0.0	0.0	0.0	0.5076115734283109	0.0012134141197489505
52-53	0.0	0.0	0.0	0.5905859865692391	0.0013289773692488505
54-55	0.0	0.0	0.0	0.6764494809476649	0.0014445406187487504
56-57	0.0	0.0	0.0	0.7648264760027135	0.0014445406187487504
58-59	0.0	0.0	0.0	0.8666088079997504	0.0014445406187487504
60-61	0.0	0.0	0.0	0.9720602731684092	0.0015023222434987005
62-63	0.0	0.0	0.0	1.0977641978119255	0.0015601038682486505
64-65	0.0	0.0	0.0	1.2093405152040788	0.0015601038682486505
66-67	0.0	0.0	0.0	1.3311730709893486	0.0015601038682486505
68-69	0.0	0.0	0.0	1.4787184497883459	0.0015601038682486505
70-71	0.0	0.0	0.0	1.6249059604057194	0.0017334487424985005
72-73	0.0	0.0	0.0	1.76863775197122	0.0021090293033731757
74-75	0.0	0.0	0.0	1.918898867133465	0.002137920115748151
76-77	0.0	0.0	0.0	2.0903658385789416	0.002137920115748151
78-79	0.0	0.0	0.0	2.2842520804273994	0.002137920115748151
80-81	0.0	0.0	0.0	2.4663797616392413	0.002137920115748151
82-83	0.0	0.0	0.0	2.6642240447830705	0.0022534833652480507
84-85	0.0	0.0	0.0	2.8800095224117586	0.0022534833652480507
86-87	0.0	0.0	0.0	3.1079869228626866	0.0022534833652480507
88-89	0.0	0.0	0.0	3.3748224659579558	0.0023112649899980006
90-91	0.0	0.0	0.0	3.6492274018954682	0.002340155802372976
92-93	0.0	0.0	0.0	3.9193853884138594	0.002426828239497901
94-95	0.0	0.0	0.0	4.201013027445116	0.002715736363247651
96-97	0.0	0.0	0.0	4.51479614064972	0.002860190425122526
98-99	0.0	0.0	0.0	4.85590996236105	0.003062426111747351
100-101	0.0	0.0	0.0	5.225105653700855	0.003062426111747351
102-103	0.0	0.0	0.0	5.611491378403771	0.003120207736497301
104-105	0.0	0.0	0.0	6.015731625154421	0.003120207736497301
106-107	0.0	0.0	0.0	6.445944712230174	0.003149098548872276
108-109	0.0	0.0	0.0	6.899125995144033	0.003206880173622226
110-111	0.0	0.0	0.0	7.381198090432865	0.003351334235497101
112-113	0.0	2.889081237497501E-5	0.0	7.876675522663687	0.003466897484997001
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3520	0.0	40.068947	1
GTACATG	9460	0.0	38.0598	1
TACATGG	9635	0.0	36.806236	2
CATGGGG	8295	0.0	35.00502	4
ACATGGG	10000	0.0	34.986797	3
ATGGGGG	5090	0.0	33.196304	5
TCAACGC	4050	0.0	32.762478	4
CAACGCA	4175	0.0	31.778809	5
GGTATCA	2400	0.0	30.995668	1
AACGCAG	4330	0.0	30.639458	6
TATCAAC	4430	0.0	30.355093	2
ATCAACG	4500	0.0	29.618452	3
GTATAAC	475	0.0	26.310427	1
ACGCAGA	5245	0.0	25.17655	7
CGCAGAG	5665	0.0	23.414299	8
TGGGGGG	4010	0.0	22.995953	6
GTATAGG	400	0.0	22.31688	1
GCAGAGT	6075	0.0	21.050789	9
AGTACAT	6535	0.0	20.759464	2
ATGGGGA	3320	0.0	20.608624	5
>>END_MODULE