FastQCFastQC Report
Mon 15 Aug 2016
SRR2921622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730654
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.11406092725640134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42000.045.996441
GGTATCA26250.036.7971531
TCAACGC52300.036.018524
CAACGCA53350.035.2769475
TATCAAC55650.034.3888852
GTACATG97850.034.3064771
ATCAACG55900.033.595183
AACGCAG56600.033.1614466
TACATGG100150.032.859912
ACATGGG99000.032.6394123
CATGGGG85750.031.839914
ATGGGGG50850.031.1550875
ACGCAGA67800.027.7713367
TACCGTA1755.632246E-727.2390067
CGCAGAG71700.026.1776568
TGGGGGG35900.024.398456
GCAGAGT80900.023.053419
AGAGTAC75050.021.23794410-11
CTAGTAC3001.3546014E-719.8727043
ACCGTAT2751.3007248E-619.5006548
Produced by FastQC (version 0.11.5)