##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730654
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.707857838712997	33.0	31.0	34.0	30.0	34.0
2	31.89825984858903	33.0	31.0	34.0	30.0	34.0
3	31.988680001895236	34.0	31.0	34.0	30.0	34.0
4	35.391634029678954	37.0	35.0	37.0	33.0	37.0
5	35.23018350288388	37.0	35.0	37.0	33.0	37.0
6	35.25237626931784	37.0	35.0	37.0	33.0	37.0
7	35.14813128447396	37.0	35.0	37.0	33.0	37.0
8	35.17951768522189	37.0	36.0	37.0	33.0	37.0
9	36.895760793318594	39.0	38.0	39.0	34.0	39.0
10-11	36.84838535027799	39.0	38.0	39.0	33.5	39.0
12-13	36.7703004182234	39.0	38.0	39.0	33.0	39.0
14-15	38.205999003844795	41.0	39.0	41.0	34.0	41.0
16-17	38.20775845431842	41.0	39.0	41.0	34.0	41.0
18-19	38.21080643502398	41.0	39.0	41.0	34.0	41.0
20-21	38.14601561028374	41.0	39.0	41.0	34.0	41.0
22-23	38.148239625020366	41.0	39.0	41.0	34.0	41.0
24-25	38.110779797694974	41.0	39.0	41.0	34.0	41.0
26-27	38.035638839421395	41.0	39.0	41.0	33.5	41.0
28-29	37.96875949785457	41.0	39.0	41.0	33.0	41.0
30-31	37.86599950076676	41.0	38.0	41.0	33.0	41.0
32-33	37.75780080824937	41.0	38.0	41.0	33.0	41.0
34-35	37.62967352226384	41.0	38.0	41.0	33.0	41.0
36-37	37.56704864172735	40.0	38.0	41.0	32.5	41.0
38-39	37.48884583515827	40.0	38.0	41.0	32.0	41.0
40-41	37.376918205487634	40.0	38.0	41.0	32.0	41.0
42-43	37.23234713582264	40.0	38.0	41.0	32.0	41.0
44-45	37.09817964769388	40.0	37.0	41.0	31.0	41.0
46-47	36.92439187729032	40.0	37.0	41.0	31.0	41.0
48-49	36.76162999652155	40.0	37.0	41.0	31.0	41.0
50-51	36.5264350933231	40.0	36.0	41.0	30.0	41.0
52-53	36.40241030269482	40.0	36.0	41.0	30.0	41.0
54-55	36.27640302452137	40.0	35.5	41.0	30.0	41.0
56-57	36.07825134313387	39.0	35.0	41.0	30.0	41.0
58-59	35.88643166109459	39.0	35.0	41.0	30.0	41.0
60-61	35.62622858179625	39.0	35.0	41.0	29.0	41.0
62-63	35.27575240342668	38.0	35.0	41.0	29.0	41.0
64-65	34.98464742230394	37.0	35.0	40.0	29.0	41.0
66-67	34.677500239793744	37.0	35.0	39.5	29.0	41.0
68-69	34.306022174276315	36.0	35.0	39.0	28.0	41.0
70-71	33.93055862119176	35.5	35.0	39.0	28.0	41.0
72-73	33.605135399681274	35.0	34.0	37.5	28.0	40.0
74-75	33.2824212696472	35.0	34.0	37.0	27.5	39.0
76-77	32.95741205347805	35.0	34.0	36.5	27.0	39.0
78-79	32.70172951959202	35.0	34.0	36.0	27.0	38.0
80-81	32.452037784560055	35.0	34.0	36.0	26.0	37.0
82-83	32.23458588487358	35.0	34.0	35.0	26.0	37.0
84-85	32.0259167921491	35.0	34.0	35.0	26.0	36.0
86-87	31.900756592594476	35.0	34.0	35.0	26.0	36.0
88-89	31.808001772740248	35.0	34.0	35.0	25.5	36.0
90-91	31.706981002557413	35.0	34.0	35.0	25.0	35.5
92-93	31.631838310835093	35.0	33.5	35.0	25.0	35.0
94-95	31.569691284335285	35.0	33.0	35.0	25.0	35.0
96-97	31.50742667222911	35.0	33.0	35.0	25.0	35.0
98-99	31.435180284447384	35.0	33.0	35.0	24.5	35.0
100-101	31.368532358287677	35.0	33.0	35.0	24.0	35.0
102-103	30.67267836320836	34.0	31.5	35.0	23.0	35.0
104-105	31.198280245502566	35.0	33.0	35.0	24.0	35.0
106-107	31.29917129593784	35.0	33.0	35.0	24.0	35.0
108-109	31.288025509431698	35.0	33.0	35.0	24.0	35.0
110-111	31.22364435641093	35.0	33.0	35.0	24.0	35.0
112-113	31.186564443268267	35.0	33.0	35.0	23.0	35.0
114-115	31.05979242529125	35.0	33.0	35.0	22.0	35.0
116-117	31.245521346265633	35.0	33.5	35.0	24.0	35.0
118-119	31.234123631875583	35.0	34.0	35.0	23.5	35.0
120-121	31.16034285304862	35.0	34.0	35.0	23.0	35.0
122-123	31.02902457683627	35.0	33.0	35.0	19.0	35.0
124-125	30.383407081947055	34.5	32.0	35.0	8.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28559.0
3	7829.0
4	4064.0
5	3216.0
6	3268.0
7	3770.0
8	3997.0
9	4352.0
10	4589.0
11	4727.0
12	5099.0
13	5930.0
14	7683.0
15	7079.0
16	4622.0
17	4121.0
18	3758.0
19	3468.0
20	3349.0
21	3472.0
22	3956.0
23	4791.0
24	6234.0
25	8117.0
26	10053.0
27	11965.0
28	14635.0
29	17570.0
30	21415.0
31	26971.0
32	35219.0
33	47866.0
34	75674.0
35	134017.0
36	312975.0
37	653183.0
38	228781.0
39	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9619479153422	17.414030261348003	11.012969149145215	27.611052674164576
2	15.71422875728998	19.317716275412003	37.79463998349733	27.173414983800686
3	18.878227784158	23.401532679102033	28.125742339060896	29.594497197679075
4	13.38081887970071	14.669202418764874	33.83510126031355	38.11487744122086
5	14.668313386980817	37.322141491572594	32.4873375144772	15.522207606969395
6	35.1590723018072	33.46190405706066	16.51965160436836	14.859372036763787
7	30.982652877765425	31.228808929602724	19.087210811442038	18.701327381189817
8	28.38130538154412	34.95913998882037	18.13933619317765	18.52021843645787
9	28.313592845797626	15.38289918132657	17.983071655310972	38.320436317564834
10-11	27.24110737750602	24.79180590598253	26.102399580027242	21.86468713648421
12-13	26.952982839125074	21.837760399879866	26.814607802016454	24.39464895897861
14-15	24.296370083060214	23.316841156518237	23.928504736923955	28.458284023497594
16-17	24.773216019978726	26.541154095176434	24.45888059011238	24.22674929473246
18-19	24.768430611262264	25.743146268618755	26.085218691096117	23.40320442902286
20-21	25.40127651372525	24.892335513834773	25.762667391798743	23.94372058064123
22-23	25.101720237061677	24.43085638573111	25.753775631578446	24.713647745628766
24-25	24.59679446471074	24.69670380088861	25.836849036357407	24.869652698043247
26-27	24.484902591305303	24.85145282402873	25.922038374085215	24.741606210580755
28-29	24.61796756519998	25.439686532748134	25.629594017495293	24.312751884556587
30-31	25.217559879707537	24.927691910768164	25.655380990173725	24.199367219350574
32-33	24.78871033777164	24.783625112575617	25.83727799452587	24.590386555126877
34-35	24.748645753970283	25.050684006563134	25.90095029081366	24.29971994865292
36-37	24.809972609705643	25.10805875622638	25.722086747489225	24.359881886578755
38-39	24.90858735685716	25.12666024890588	25.677042945451728	24.28770944878523
40-41	24.84258340609234	24.896639833953447	25.807393856115713	24.4533829038385
42-43	24.73213622748998	24.889625824249006	25.629748920960793	24.74848902730022
44-45	24.455598129863645	25.04973797635344	25.842107422472033	24.65255647131088
46-47	24.51782061505391	24.888794508199563	25.66627951322678	24.927105363519743
48-49	24.558944193410454	25.11718343714969	25.588430735805666	24.73544163363419
50-51	24.61727054650955	25.243054763052847	25.54878184428374	24.59089284615387
52-53	24.687471289539538	25.27745500077717	25.397294990699976	24.637778718983313
54-55	24.386659848084296	24.85662197028138	25.79383033864663	24.962887842987694
56-57	24.583016495593547	25.000260018813808	25.472194165871205	24.944529319721447
58-59	24.524649631972366	25.111367470326805	25.62681128331093	24.7371716143899
60-61	24.896578084521252	24.970683039672586	25.683332914416436	24.44940596138973
62-63	24.716506118845256	25.09844641019463	25.63769872018222	24.547348750777893
64-65	24.53182438546353	24.863837601276742	25.622597006680714	24.981741006579018
66-67	24.459447043752157	25.116911063227604	25.535236847108123	24.888405045912116
68-69	24.41068885648604	25.0447882536178	25.673282799143788	24.87124009075237
70-71	24.414441014784003	24.888134774484097	25.829079642724658	24.868344568007238
72-73	24.50370330136402	25.162832005676584	25.4432231723003	24.890241520659092
74-75	24.407859058624545	25.19529043574535	25.462024363740483	24.934826141889623
76-77	24.318707704984295	24.825171693603444	25.632104905362308	25.224015696049957
78-79	24.23022530519558	24.8656792741466	25.78241443617364	25.121680984484172
80-81	24.37428278558279	25.141391635763128	25.371911427703054	25.11241415095103
82-83	24.484009763336175	25.16717170666537	25.32749590305081	25.02132262694764
84-85	24.439516232714702	25.26401581393548	25.2283350730704	25.068132880279414
86-87	24.192877374680325	25.31306084289523	25.374973853814804	25.119087928609645
88-89	24.074761041329364	24.979805088770938	25.529516928478234	25.415916941421468
90-91	24.330827137181306	24.840232884406035	25.57799293211082	25.250947046301842
92-93	24.249387803685774	25.106404861977033	25.56597101442576	25.078236319911433
94-95	24.149818473466734	24.90643423874134	25.475427322646873	25.46831996514506
96-97	23.923418573079086	24.967894611697428	25.550197514949048	25.558489300274438
98-99	23.974320564205666	24.995297997192377	25.41095215629082	25.619429282311142
100-101	23.876985232172345	25.003986932107747	25.624243782986085	25.49478405273382
102-103	23.789462371058963	24.866147474107787	25.67272956300458	25.671660591828672
104-105	24.00144685188374	24.9706758254394	25.525928348474046	25.501948974202815
106-107	23.912306759352646	24.836007815576433	25.598092966992393	25.653592458078528
108-109	23.876718649285902	24.812428908757923	25.687900310324014	25.62295213163216
110-111	23.69474047931069	25.14702551408371	25.563082583904794	25.595151422700805
112-113	23.717741481150842	25.08442649355227	25.556677486316538	25.641154538980356
114-115	23.693297447092256	25.154739191080367	25.448558752933863	25.703404608893514
116-117	23.487915341518434	25.018490440442292	25.64664537184276	25.846948846196515
118-119	23.59131928343496	24.89972509812249	25.656449215381215	25.852506403061337
120-121	23.369358421749226	24.961436773788506	25.768382574940073	25.9008222295222
122-123	23.304822644834637	25.05547811138959	25.592881235960736	26.046818007815038
124-125	23.2464338131629	25.00629731117104	25.530673183287185	26.21659569237888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	15.0
2	28.0
3	67.5
4	69.0
5	73.5
6	153.0
7	353.0
8	606.5
9	674.0
10	563.0
11	470.5
12	461.5
13	496.0
14	511.0
15	543.0
16	604.5
17	631.5
18	683.5
19	712.0
20	734.5
21	810.0
22	889.0
23	1005.0
24	1148.5
25	1391.0
26	1776.0
27	2229.0
28	2925.0
29	3959.5
30	5348.0
31	7023.5
32	9364.0
33	11910.0
34	14284.0
35	17086.5
36	20829.0
37	24769.0
38	28312.0
39	32241.0
40	36351.0
41	39879.5
42	43789.0
43	49404.5
44	55391.0
45	61043.5
46	65095.0
47	69012.5
48	75028.5
49	79488.5
50	82154.0
51	83462.5
52	82261.0
53	81452.5
54	80949.5
55	77862.5
56	72436.0
57	66032.0
58	60364.0
59	54492.5
60	46802.0
61	39236.0
62	32521.5
63	25075.5
64	18292.0
65	13802.0
66	10438.0
67	7972.0
68	6099.5
69	4641.0
70	3653.5
71	2762.5
72	2124.5
73	1751.0
74	1320.5
75	933.0
76	641.5
77	397.5
78	225.5
79	114.5
80	56.0
81	29.5
82	21.0
83	11.5
84	5.5
85	2.5
86	2.0
87	3.5
88	2.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022765960151480306
2	0.0014445406187487504
3	0.02779296150472596
4	0.16352199804235854
5	0.019992442163482706
6	0.06506210946844372
7	0.049518852410707165
8	0.04177611469421386
9	0.03108651411547311
10-11	0.05882169399544912
12-13	0.0539969283288283
14-15	0.05460363538870277
16-17	0.04703424254645931
18-19	0.03530457272221946
20-21	0.022794850963855282
22-23	0.002860190425122526
24-25	0.004911438103745752
26-27	0.003409115860247051
28-29	0.004304731043871276
30-31	0.002224592552873076
32-33	0.008696134524867477
34-35	0.004535857542871077
36-37	0.006587105221494301
38-39	0.0023112649899980006
40-41	0.0031779893612472513
42-43	0.003669133171621826
44-45	0.004911438103745752
46-47	0.004275840231496302
48-49	0.0019645752414983006
50-51	0.0014734314311237254
52-53	7.511611217493503E-4
54-55	0.005142564602745552
56-57	5.778162474995002E-4
58-59	5.489254351245252E-4
60-61	2.889081237497501E-5
62-63	9.822876207491503E-4
64-65	0.0
66-67	0.003755805608746751
68-69	6.067070598744752E-4
70-71	0.0
72-73	0.0020801384909982005
74-75	0.005229237039870477
76-77	0.0015889946806236256
78-79	8.667243712492503E-5
80-81	0.0
82-83	0.005662599225495102
84-85	0.0016467763053735754
86-87	0.0
88-89	0.0011556324949990003
90-91	5.778162474995002E-4
92-93	0.0
94-95	0.0030624261117473514
96-97	0.0014734314311237254
98-99	4.911438103745752E-4
100-101	0.0
102-103	0.0010400692454991002
104-105	0.0
106-107	4.333621856246251E-4
108-109	0.002513500676622826
110-111	1.1556324949990003E-4
112-113	3.7558056087467513E-4
114-115	0.0
116-117	0.0017334487424985005
118-119	0.0021090293033731757
120-121	0.0031490985488722765
122-123	9.533968083741752E-4
124-125	0.10062669950203795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1730654.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.44663446330543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98793009642246	48.350011720148764
2	10.923212777301945	13.205428998289563
3	3.5242434816565487	6.390859724861409
4	1.681552987124932	4.0657687497368045
5	0.9621793212331716	2.908025085936643
6	0.630737339659725	2.2875569647661473
7	0.41906180318729774	1.773161294435736
8	0.3066756492809377	1.483000869670536
9	0.2399197075433112	1.3052104976172307
>10	1.2427374037181764	13.09316818633916
>50	0.05832719786865693	2.394023288783987
>100	0.02246251052696496	2.203807856926334
>500	5.758346855133621E-4	0.21992392636342026
>1k	3.838897903422414E-4	0.32005283612423885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1974	0.11406092725640134	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	5.778162474995002E-5
3	0.0	0.0	0.0	0.0	5.778162474995002E-5
4	0.0	0.0	0.0	0.0	5.778162474995002E-5
5	0.0	0.0	0.0	0.0	5.778162474995002E-5
6	0.0	0.0	0.0	0.0	1.1556324949990003E-4
7	0.0	0.0	0.0	0.0	1.1556324949990003E-4
8	0.0	0.0	0.0	0.0	1.1556324949990003E-4
9	0.0	0.0	0.0	0.0	1.7334487424985006E-4
10-11	0.0	0.0	0.0	0.0	1.7334487424985006E-4
12-13	0.0	0.0	0.0	0.0	1.7334487424985006E-4
14-15	0.0	0.0	0.0	0.0	2.3112649899980007E-4
16-17	0.0	0.0	0.0	0.0	2.889081237497501E-4
18-19	0.0	0.0	0.0	2.889081237497501E-5	2.889081237497501E-4
20-21	0.0	0.0	0.0	5.778162474995002E-5	3.177989361247251E-4
22-23	0.0	0.0	0.0	2.0223568662482508E-4	3.466897484997001E-4
24-25	0.0	0.0	0.0	3.177989361247251E-4	3.466897484997001E-4
26-27	0.0	0.0	0.0	6.355978722494502E-4	4.0447137324965015E-4
28-29	0.0	0.0	0.0	0.002166810928123126	4.0447137324965015E-4
30-31	0.0	0.0	0.0	0.008811697774367378	4.333621856246251E-4
32-33	0.0	0.0	0.0	0.025337242452853084	4.911438103745752E-4
34-35	0.0	0.0	0.0	0.048276547478583245	5.200346227495501E-4
36-37	0.0	0.0	0.0	0.0753761294863098	5.200346227495501E-4
38-39	0.0	0.0	0.0	0.12425938402476752	5.200346227495501E-4
40-41	0.0	0.0	0.0	0.19033267192633535	6.355978722494502E-4
42-43	0.0	0.0	0.0	0.24759426205353582	6.644886846244253E-4
44-45	0.0	0.0	0.0	0.30419136349611187	6.933794969994002E-4
46-47	0.0	0.0	0.0	0.36546877654343385	6.933794969994002E-4
48-49	0.0	0.0	0.0	0.4318598633811265	6.933794969994002E-4
50-51	0.0	0.0	0.0	0.5094316946079344	6.933794969994002E-4
52-53	0.0	0.0	0.0	0.5939951024294863	8.089427464993003E-4
54-55	0.0	0.0	0.0	0.6803497406182865	9.245059959992003E-4
56-57	0.0	0.0	0.0	0.768726735673335	0.0010111784331241253
58-59	0.0	0.0	0.0	0.8707979757941218	0.0010400692454991002
60-61	0.0	0.0	0.0	0.9773184010206546	0.0010978508702490504
62-63	0.0	0.0	0.0	1.102964544039421	0.0011556324949990003
64-65	0.0	0.0	0.0	1.216158746924573	0.0011556324949990003
66-67	0.0	0.0	0.0	1.339753642264716	0.0011556324949990003
68-69	0.0	0.0	0.0	1.4893213779299617	0.0011556324949990003
70-71	0.0	0.0	0.0	1.6384557514095828	0.0011845233073739755
72-73	0.0	0.0	0.0	1.783920991717582	0.0014445406187487504
74-75	0.0	0.0	0.0	1.9361466821213251	0.0014445406187487504
76-77	0.0	0.0	0.0	2.1095782288083003	0.0014445406187487504
78-79	0.0	0.0	0.0	2.306180207020005	0.0014445406187487504
80-81	0.0	0.0	0.0	2.4907058256589707	0.0014734314311237256
82-83	0.0	0.0	0.0	2.6931148571580454	0.0015023222434987005
84-85	0.0	0.0	0.0	2.9129161577068556	0.0015312130558736755
86-87	0.0	0.0	0.0	3.1464117033214034	0.0015889946806236254
88-89	0.0	0.0	0.0	3.419805460825792	0.0016178854929986006
90-91	0.0	0.0	0.0	3.7008263927971736	0.0016178854929986006
92-93	5.778162474995002E-5	0.0	0.0	3.9762136163554356	0.0016756671177485505
94-95	5.778162474995002E-5	0.0	0.0	4.261770405869688	0.0018490119919984005
96-97	5.778162474995002E-5	0.0	0.0	4.5792804338706645	0.0018779028043733755
98-99	5.778162474995002E-5	0.0	0.0	4.925681274246614	0.0019067936167483507
100-101	5.778162474995002E-5	0.0	0.0	5.3009440361851645	0.0019645752414983006
102-103	5.778162474995002E-5	0.0	0.0	5.691490037870077	0.0020223568662482505
104-105	5.778162474995002E-5	0.0	0.0	6.099514981041849	0.0020801384909982005
106-107	5.778162474995002E-5	0.0	0.0	6.535795138716347	0.0020801384909982005
108-109	5.778162474995002E-5	0.0	0.0	6.995823544163073	0.0021957017404981008
110-111	5.778162474995002E-5	0.0	0.0	7.486360647477774	0.0023112649899980006
112-113	5.778162474995002E-5	0.0	0.0	7.991805409977962	0.002369046614747951
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4200	0.0	45.99644	1
GGTATCA	2625	0.0	36.797153	1
TCAACGC	5230	0.0	36.01852	4
CAACGCA	5335	0.0	35.276947	5
TATCAAC	5565	0.0	34.388885	2
GTACATG	9785	0.0	34.306477	1
ATCAACG	5590	0.0	33.59518	3
AACGCAG	5660	0.0	33.161446	6
TACATGG	10015	0.0	32.85991	2
ACATGGG	9900	0.0	32.639412	3
CATGGGG	8575	0.0	31.83991	4
ATGGGGG	5085	0.0	31.155087	5
ACGCAGA	6780	0.0	27.771336	7
TACCGTA	175	5.632246E-7	27.239006	7
CGCAGAG	7170	0.0	26.177656	8
TGGGGGG	3590	0.0	24.39845	6
GCAGAGT	8090	0.0	23.05341	9
AGAGTAC	7505	0.0	21.237944	10-11
CTAGTAC	300	1.3546014E-7	19.872704	3
ACCGTAT	275	1.3007248E-6	19.500654	8
>>END_MODULE