FastQCFastQC Report
Mon 15 Aug 2016
SRR2921621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155415
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.039.5249251
GTACATG102700.038.432621
GTATCAA36850.037.3191221
TACATGG105200.036.9996722
CATGGGG90700.035.696654
ACATGGG107900.035.246443
TCAACGC39950.032.4769744
CAACGCA40450.032.2189255
ATGGGGG54500.030.9012035
AACGCAG43600.029.7512386
TATCAAC44850.029.592272
ATCAACG44600.029.3578133
ACGCAGA53800.023.9967277
GAGTACA71500.023.8131541
AGTACAT71950.022.7477152
CGCAGAG58350.022.1250028
ATGGGGA41350.021.587445
CTAGTAC3551.8280844E-920.1181813
ATGGGGC24200.019.9184285
GTATAGG5550.019.3079621
Produced by FastQC (version 0.11.5)