##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921621_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2155415
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.884292352052853	31.0	31.0	34.0	26.0	34.0
2	30.013380717866397	31.0	31.0	34.0	26.0	34.0
3	30.33091307242457	31.0	31.0	34.0	26.0	34.0
4	33.74395371656966	37.0	35.0	37.0	30.0	37.0
5	33.810198500056835	37.0	35.0	37.0	30.0	37.0
6	33.82857407970159	37.0	35.0	37.0	30.0	37.0
7	33.723206435883576	37.0	35.0	37.0	29.0	37.0
8	33.71871959692217	37.0	35.0	37.0	29.0	37.0
9	35.415484257092025	39.0	37.0	39.0	30.0	39.0
10-11	35.437612710313324	39.0	37.0	39.0	30.0	39.0
12-13	35.38064688238692	39.0	37.0	39.0	30.0	39.0
14-15	36.68394508714099	40.0	38.0	41.0	29.5	41.0
16-17	36.65085888332409	40.0	38.0	41.0	29.0	41.0
18-19	36.60562745457371	40.0	38.0	41.0	29.0	41.0
20-21	36.65022095512929	40.0	38.0	41.0	29.0	41.0
22-23	36.57967467981804	40.0	38.0	41.0	28.5	41.0
24-25	36.56461563086459	40.0	38.0	41.0	29.0	41.0
26-27	36.514945149773936	40.0	38.0	41.0	29.0	41.0
28-29	36.45372515269681	40.0	38.0	41.0	28.5	41.0
30-31	36.362128638800414	40.0	38.0	41.0	28.0	41.0
32-33	36.26716154429657	40.0	37.5	41.0	28.0	41.0
34-35	36.1440945247203	40.0	37.0	41.0	27.0	41.0
36-37	36.04769777513843	40.0	37.0	41.0	27.0	41.0
38-39	35.93560080077387	40.0	37.0	41.0	26.5	41.0
40-41	35.82617059823746	40.0	36.5	41.0	26.0	41.0
42-43	35.60240788896802	40.0	36.0	41.0	25.5	41.0
44-45	35.2931681369945	40.0	35.0	41.0	24.0	41.0
46-47	35.055914986209146	39.5	35.0	41.0	24.0	41.0
48-49	34.86883685972307	39.0	35.0	41.0	23.0	41.0
50-51	34.66968913179133	39.0	35.0	41.0	23.0	41.0
52-53	34.467174070886585	39.0	34.5	40.5	22.0	41.0
54-55	34.225568394021565	38.5	34.0	40.0	21.5	41.0
56-57	33.9741175133327	38.0	34.0	40.0	20.0	41.0
58-59	33.973835665057535	38.0	34.0	40.0	20.5	41.0
60-61	33.75820851204988	37.5	34.0	40.0	20.0	41.0
62-63	33.44893953136635	37.0	34.0	40.0	18.5	41.0
64-65	33.14759593860116	36.5	33.5	39.5	18.0	41.0
66-67	32.81899657374566	36.0	33.0	39.0	16.5	41.0
68-69	32.485537819863	35.5	33.0	39.0	14.0	40.5
70-71	32.11743515749867	35.0	33.0	38.0	12.0	40.0
72-73	31.766071267018184	35.0	33.0	37.0	8.0	39.5
74-75	31.39978008875321	35.0	32.0	37.0	7.0	39.0
76-77	31.098856600701026	35.0	32.0	36.0	6.5	39.0
78-79	30.829586877701047	35.0	32.0	36.0	2.0	37.5
80-81	30.56752899093678	35.0	32.0	35.0	2.0	37.0
82-83	30.348536360747232	35.0	31.5	35.0	2.0	36.5
84-85	30.13778924244287	35.0	31.0	35.0	2.0	36.0
86-87	29.969706993780783	35.0	31.0	35.0	2.0	36.0
88-89	29.81289751625557	35.0	31.0	35.0	2.0	35.5
90-91	29.628808837277276	35.0	31.0	35.0	2.0	35.0
92-93	29.552457647367213	34.0	31.0	35.0	2.0	35.0
94-95	29.42813170549523	34.0	31.0	35.0	2.0	35.0
96-97	29.339013136681334	34.0	31.0	35.0	2.0	35.0
98-99	29.245392882577136	34.0	30.0	35.0	2.0	35.0
100-101	29.133657323531665	34.0	30.0	35.0	2.0	35.0
102-103	28.268262492373857	33.5	29.0	34.5	2.0	35.0
104-105	29.170665973837984	34.0	30.5	35.0	2.0	35.0
106-107	29.433455506248215	35.0	31.0	35.0	2.0	35.0
108-109	29.432667490947217	35.0	31.0	35.0	2.0	35.0
110-111	29.36883477195807	35.0	31.0	35.0	2.0	35.0
112-113	29.2831526643361	35.0	31.0	35.0	2.0	35.0
114-115	29.165661137182397	35.0	31.0	35.0	2.0	35.0
116-117	29.04897966284915	35.0	31.0	35.0	2.0	35.0
118-119	28.763524425690644	35.0	31.0	35.0	2.0	35.0
120-121	28.644211903508143	35.0	30.5	35.0	2.0	35.0
122-123	28.412175381539054	34.0	30.0	35.0	2.0	35.0
124-125	27.66528533948219	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89006.0
3	15384.0
4	7917.0
5	6547.0
6	6853.0
7	7473.0
8	7454.0
9	7970.0
10	8585.0
11	8711.0
12	8672.0
13	8713.0
14	8747.0
15	8136.0
16	6975.0
17	6562.0
18	6021.0
19	5593.0
20	5587.0
21	5856.0
22	6789.0
23	8925.0
24	12585.0
25	16154.0
26	18423.0
27	21009.0
28	25004.0
29	29910.0
30	36474.0
31	45389.0
32	58797.0
33	80503.0
34	122145.0
35	204021.0
36	417642.0
37	645838.0
38	168952.0
39	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91353862360691	17.070309327628333	11.670823984202967	25.34532806456179
2	16.592667106843795	18.95523973955001	38.436604053558085	26.015489100048118
3	19.928257770914843	22.969205870780467	28.054821419291613	29.047714939013076
4	13.657810936178327	14.4080509396676	34.90042813188893	37.03370999226514
5	15.15037618407266	37.03002512224706	32.97093076672504	14.848667926955233
6	35.21130423314331	33.39017399558277	16.31429171147977	15.084230059794148
7	30.59680325254732	31.0456625816398	19.318730975471734	19.038803190341145
8	27.942969685238744	34.28150223523148	19.228792919299114	18.546735160230657
9	27.722700599344268	15.328827229674385	18.045808186629728	38.90266398435161
10-11	26.853238391874896	24.90765024532217	26.46434357172366	21.774767791079277
12-13	26.513793416252906	22.244860601895795	26.833030143510367	24.408315838340936
14-15	24.082888978610644	23.675126809324258	23.631781920447743	28.61020229161736
16-17	24.771282158859727	26.83644327716321	24.294133522255915	24.098141041721153
18-19	24.792336820823106	26.219969686849982	25.485812206531367	23.501881285795545
20-21	25.33941964403807	25.246974054439868	25.393949547541027	24.01965675398103
22-23	25.30840790972304	24.806345823428234	25.20379520415258	24.68145106269615
24-25	24.8950284986459	25.16594140902095	25.219242218882314	24.719787873450834
26-27	24.684411959448216	25.381185422319263	25.336463626930644	24.597938991301877
28-29	24.797737322276777	25.605349143175822	25.207240772943855	24.389672761603546
30-31	25.134307149597735	25.34334668844384	25.143681328051926	24.378664833906498
32-33	24.71268581908906	25.366370828816255	25.290609174747907	24.63033417734678
34-35	24.81093773860509	25.52327764546759	25.348244493139543	24.317540122787776
36-37	24.92739138635299	25.509095477282013	25.227871221469556	24.335641914895444
38-39	24.90977769837421	25.576264933606502	25.164632208097153	24.349325159922135
40-41	24.89053620607669	25.433846086723033	25.2329158852521	24.44270182194818
42-43	24.79092761709109	25.331523843753097	25.21708886414466	24.660459675011154
44-45	24.637595415419597	25.382636133732394	25.42841233382056	24.55135611702745
46-47	24.687889659371294	25.324894996443035	25.39710161875553	24.590113725430143
48-49	24.576153986092393	25.534808000029702	25.303880838614585	24.58515717526332
50-51	24.615898672848207	25.54435963388756	25.321392563819657	24.518349129444577
52-53	24.744163750049886	25.46930382654553	25.30061803504024	24.485914388364346
54-55	24.520811343909294	25.241024196506736	25.43829383802682	24.799870621557147
56-57	24.62854947159054	25.39769349605984	25.21958472533985	24.75417230700977
58-59	24.609924404799745	25.44325348452064	25.34682795746326	24.599994153216358
60-61	24.856038806062962	25.353783270941253	25.377310815570503	24.412867107425285
62-63	24.63252175773293	25.458026700251775	25.390924550221854	24.518526991793443
64-65	24.544329866289736	25.178008109670813	25.458408006095713	24.819254017943738
66-67	24.589524498124668	25.499299708838517	25.262133507331015	24.6490422857058
68-69	24.55863139012579	25.395253133938134	25.34699140314717	24.6991240727889
70-71	24.491089895692923	25.301242847222994	25.50004455980483	24.707622697279252
72-73	24.6410091674364	25.48642912679072	25.285752967160246	24.586808738612632
74-75	24.46800909054633	25.48109090267937	25.336046763047243	24.71485324372705
76-77	24.569068822078524	25.25236401669272	25.401407795518615	24.77715936571014
78-79	24.490590501541373	25.212272326705175	25.497088723962214	24.80004844779124
80-81	24.590513481106406	25.508998541078114	25.214174743946216	24.68631323386926
82-83	24.59105211659345	25.481167363802182	25.33324794612438	24.594532573479984
84-85	24.594897888399807	25.486151533999806	25.20814517115035	24.710805406450035
86-87	24.438869879064104	25.515608628387774	25.358626763667818	24.6868947288803
88-89	24.48796906247484	25.29472623970178	25.369160436553777	24.8481442612696
90-91	24.637935710923053	25.23564718665392	25.382099375420395	24.74431772700263
92-93	24.536536319173884	25.42128738390499	25.429639125869567	24.612537171051564
94-95	24.58482112096615	25.205602951578875	25.45087186230605	24.75870406514892
96-97	24.441141178206546	25.25748153455097	25.48287648032162	24.818500806920866
98-99	24.439414913348465	25.255182586130143	25.39497942369647	24.910423076824927
100-101	24.406183858835746	25.290606426389488	25.493488629664697	24.80972108511007
102-103	24.2373381499578	25.238863218474535	25.614794695712412	24.909003935855257
104-105	24.277923567446383	25.354612232474928	25.496192662593632	24.87127153748506
106-107	24.197792052036863	25.246114981244737	25.615049677669287	24.941043289049105
108-109	24.18615938501829	25.289856114807474	25.65517202978394	24.868812470390296
110-111	24.09887386055079	25.482834173675318	25.59896259231293	24.81932937346097
112-113	24.074698703506435	25.592339441539014	25.602013035457784	24.73094881949677
114-115	24.110128242129754	25.61896153478518	25.534489142710783	24.736421080374285
116-117	23.98613826978455	25.42592365330549	25.680019802471737	24.907918274438224
118-119	24.061398156352492	25.400249048504907	25.639480771319924	24.898872023822676
120-121	23.95717804137025	25.575238257400805	25.705794706710783	24.76178899451816
122-123	23.94641365871764	25.60870372088707	25.621810336828432	24.823072283566855
124-125	23.93905108350992	25.495236707857465	25.693294157782276	24.872418050850335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	502.0
1	402.0
2	268.5
3	205.0
4	165.5
5	154.0
6	157.5
7	177.0
8	205.0
9	241.5
10	265.0
11	264.5
12	275.0
13	325.5
14	377.5
15	414.5
16	454.5
17	480.0
18	537.0
19	616.5
20	702.5
21	793.0
22	929.5
23	1142.5
24	1373.0
25	1710.5
26	2294.5
27	3130.5
28	4333.0
29	5894.0
30	7985.5
31	10339.0
32	13277.0
33	16422.0
34	19759.0
35	23602.0
36	28085.0
37	32778.5
38	37483.0
39	42300.0
40	47028.5
41	51754.5
42	56435.5
43	63275.5
44	70508.0
45	76845.5
46	81733.5
47	85997.5
48	92403.0
49	96908.0
50	98900.5
51	99671.0
52	98452.5
53	97335.0
54	96605.0
55	93682.0
56	87613.0
57	80109.5
58	73360.5
59	66569.5
60	57371.5
61	48064.0
62	40127.0
63	31266.0
64	23159.0
65	17904.0
66	13948.0
67	10874.0
68	8567.0
69	6689.5
70	5276.0
71	4023.0
72	3033.0
73	2447.0
74	1879.5
75	1428.5
76	1067.0
77	678.0
78	455.5
79	335.0
80	225.5
81	137.0
82	120.0
83	104.5
84	78.5
85	61.0
86	42.0
87	34.0
88	32.5
89	35.5
90	34.5
91	31.0
92	31.5
93	19.5
94	14.5
95	18.0
96	15.5
97	10.5
98	6.5
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026908971126210036
2	0.01029964067244591
3	0.00923256078295827
4	0.011041957117306876
5	0.016377356564745073
6	0.09404221460832368
7	0.0597564738113078
8	0.048807306249608544
9	0.02598107557013383
10-11	0.051266229473210495
12-13	0.012735366507145955
14-15	0.028068840571305296
16-17	0.034355332963721605
18-19	0.04196407652354651
20-21	0.029414289127615796
22-23	0.03716221702085213
24-25	0.0307829350728282
26-27	0.045675658747851344
28-29	0.04530450052542086
30-31	0.025957878181231922
32-33	0.02927510479420436
34-35	0.044817355358480845
36-37	0.009070179060644935
38-39	0.021341597789752784
40-41	0.04340231463546463
42-43	0.022246295956927085
44-45	0.016934093898390797
46-47	0.022640651568259475
48-49	0.028903946571773885
50-51	0.028416801404833873
52-53	0.024171679235785223
54-55	0.023035007179591865
56-57	0.025609917347703345
58-59	0.017815594676663195
60-61	0.02291902023508234
62-63	0.022803033290572813
64-65	0.021202413456341357
66-67	0.027697682348874812
68-69	0.023127796735199487
70-71	0.04671954124843708
72-73	0.020900847400616585
74-75	0.009696508560996374
76-77	0.015936606175608874
78-79	0.02403249490237379
80-81	0.018511516343720353
82-83	0.02438045573590237
84-85	0.011529102284246884
86-87	0.018047568565682248
88-89	0.022803033290572813
90-91	0.020668873511597536
92-93	0.00816548089347063
94-95	0.010647601505974488
96-97	0.014335986341377414
98-99	0.0067040453926506035
100-101	0.010369232839151624
102-103	0.0047322673359886614
104-105	0.0047786621137924715
106-107	9.27895556076208E-4
108-109	0.01364006467432026
110-111	0.001971778056661942
112-113	0.0029228710016400553
114-115	0.01266577434044024
116-117	0.005961728947789637
118-119	0.27996928665709386
120-121	0.0011830668339971654
122-123	6.959216670571561E-4
124-125	0.3152989099546955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2155415.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.0419960530547
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30238068705502	49.01817604950109
2	10.512167896903494	12.833674225436628
3	3.535259701506995	6.473979262377401
4	1.6938216305975518	4.135770131580578
5	0.9892309806394861	3.0192316807877475
6	0.637077987077481	2.333310718360298
7	0.4641905606902297	1.9834582861462775
8	0.30904396893091074	1.509172858536081
9	0.2585922791380713	1.4206489994246916
>10	1.2234141499826938	12.973102012044253
>50	0.05644487763763141	2.317434161026912
>100	0.017692827338481973	1.7353784112683042
>500	6.824525019578824E-4	0.2466632035097964
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.63947778038104E-5
2	0.0	0.0	0.0	0.0	4.63947778038104E-5
3	0.0	0.0	0.0	0.0	4.63947778038104E-5
4	0.0	0.0	0.0	0.0	4.63947778038104E-5
5	0.0	4.63947778038104E-5	0.0	0.0	4.63947778038104E-5
6	0.0	4.63947778038104E-5	0.0	0.0	9.27895556076208E-5
7	0.0	4.63947778038104E-5	0.0	4.63947778038104E-5	9.27895556076208E-5
8	0.0	4.63947778038104E-5	0.0	4.63947778038104E-5	9.27895556076208E-5
9	0.0	4.63947778038104E-5	0.0	9.27895556076208E-5	1.391843334114312E-4
10-11	0.0	4.63947778038104E-5	0.0	9.27895556076208E-5	1.391843334114312E-4
12-13	0.0	4.63947778038104E-5	0.0	9.27895556076208E-5	2.0877650011714681E-4
14-15	0.0	4.63947778038104E-5	0.0	9.27895556076208E-5	2.783686668228624E-4
16-17	0.0	4.63947778038104E-5	0.0	1.1598694450952601E-4	2.783686668228624E-4
18-19	0.0	4.63947778038104E-5	0.0	1.855791112152416E-4	2.783686668228624E-4
20-21	0.0	4.63947778038104E-5	0.0	1.855791112152416E-4	3.247634446266728E-4
22-23	0.0	4.63947778038104E-5	0.0	2.0877650011714681E-4	3.247634446266728E-4
24-25	0.0	4.63947778038104E-5	0.0	5.103425558419144E-4	3.711582224304832E-4
26-27	0.0	4.63947778038104E-5	0.0	6.95921667057156E-4	3.711582224304832E-4
28-29	0.0	4.63947778038104E-5	0.0	0.003804371779912453	3.711582224304832E-4
30-31	0.0	4.63947778038104E-5	0.0	0.013083327340674534	3.9435561133238845E-4
32-33	0.0	4.63947778038104E-5	0.0	0.030852527239533918	4.1755300023429363E-4
34-35	0.0	4.63947778038104E-5	0.0	0.052866849307441954	4.1755300023429363E-4
36-37	0.0	4.63947778038104E-5	0.0	0.0864798658263026	4.1755300023429363E-4
38-39	0.0	4.63947778038104E-5	0.0	0.1435686399138913	4.1755300023429363E-4
40-41	0.0	4.63947778038104E-5	0.0	0.20937963222859635	4.1755300023429363E-4
42-43	0.0	4.63947778038104E-5	0.0	0.2688345399841794	4.6394777803810403E-4
44-45	0.0	4.63947778038104E-5	0.0	0.3347615192433939	4.871451669400092E-4
46-47	0.0	4.63947778038104E-5	0.0	0.4101762305634878	5.335399447438196E-4
48-49	0.0	4.63947778038104E-5	0.0	0.4895113006080036	5.567373336457248E-4
50-51	0.0	4.63947778038104E-5	0.0	0.5723955711545108	5.567373336457248E-4
52-53	0.0	4.63947778038104E-5	0.0	0.6602208855371239	5.567373336457248E-4
54-55	0.0	4.63947778038104E-5	0.0	0.7494380432538513	6.263295003514404E-4
56-57	0.0	4.63947778038104E-5	0.0	0.8482589199759676	6.727242781552508E-4
58-59	0.0	4.63947778038104E-5	0.0	0.9551988828137505	7.191190559590612E-4
60-61	0.0	4.63947778038104E-5	0.0	1.0685181275995574	7.423164448609664E-4
62-63	0.0	4.63947778038104E-5	0.0	1.1887733916670338	7.887112226647768E-4
64-65	0.0	4.63947778038104E-5	0.0	1.3036236641203667	7.887112226647768E-4
66-67	0.0	4.63947778038104E-5	0.0	1.4328331203039786	8.11908611566682E-4
68-69	0.0	4.63947778038104E-5	0.0	1.5797421842197443	8.351060004685873E-4
70-71	0.0	4.63947778038104E-5	0.0	1.7394794041982635	8.815007782723976E-4
72-73	0.0	6.95921667057156E-5	0.0	1.8941363960072655	0.001043882500585734
74-75	0.0	9.27895556076208E-5	0.0	2.0558453940424464	0.0010670798894876392
76-77	0.0	9.27895556076208E-5	0.0	2.238687213367263	0.0010902772783895444
78-79	0.0	9.27895556076208E-5	0.0	2.4454455406499447	0.0011134746672914497
80-81	0.0	9.27895556076208E-5	0.0	2.6561010292681457	0.001136672056193355
82-83	0.0	9.27895556076208E-5	0.0	2.8757339073913837	0.0012062642228990704
84-85	0.0	9.27895556076208E-5	0.0	3.0981736695717528	0.0012062642228990704
86-87	0.0	9.27895556076208E-5	0.0	3.343810820654027	0.0012294616118009756
88-89	0.0	9.27895556076208E-5	0.0	3.6193262086419553	0.001275856389604786
90-91	0.0	9.27895556076208E-5	0.0	3.9175286429759466	0.0012990537785066913
92-93	0.0	9.27895556076208E-5	0.0	4.221205661090787	0.0013454485563105017
94-95	0.0	9.27895556076208E-5	0.0	4.5270400363735055	0.001484632889721933
96-97	0.0	9.27895556076208E-5	0.0	4.853798456445743	0.0015310276675257434
98-99	0.0	9.27895556076208E-5	0.0	5.218646989094907	0.0015310276675257434
100-101	0.0	9.27895556076208E-5	0.0	5.613002600427295	0.0015310276675257434
102-103	0.0	9.27895556076208E-5	0.0	6.0222045406569045	0.0015774224453295538
104-105	0.0	9.27895556076208E-5	0.0	6.443190754448679	0.001623817223133364
106-107	0.0	9.27895556076208E-5	0.0	6.89212982186725	0.001623817223133364
108-109	0.0	9.27895556076208E-5	0.0	7.365147778966	0.0016702120009371745
110-111	0.0	9.27895556076208E-5	0.0	7.872265897750549	0.0016702120009371745
112-113	0.0	9.27895556076208E-5	0.0	8.387271128761746	0.001716606778740985
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	39.524925	1
GTACATG	10270	0.0	38.43262	1
GTATCAA	3685	0.0	37.319122	1
TACATGG	10520	0.0	36.999672	2
CATGGGG	9070	0.0	35.69665	4
ACATGGG	10790	0.0	35.24644	3
TCAACGC	3995	0.0	32.476974	4
CAACGCA	4045	0.0	32.218925	5
ATGGGGG	5450	0.0	30.901203	5
AACGCAG	4360	0.0	29.751238	6
TATCAAC	4485	0.0	29.59227	2
ATCAACG	4460	0.0	29.357813	3
ACGCAGA	5380	0.0	23.996727	7
GAGTACA	7150	0.0	23.813154	1
AGTACAT	7195	0.0	22.747715	2
CGCAGAG	5835	0.0	22.125002	8
ATGGGGA	4135	0.0	21.58744	5
CTAGTAC	355	1.8280844E-9	20.118181	3
ATGGGGC	2420	0.0	19.918428	5
GTATAGG	555	0.0	19.307962	1
>>END_MODULE