FastQCFastQC Report
Mon 15 Aug 2016
SRR2921621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155415
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.10898133306115063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45850.050.7390141
GGTATCA26250.043.1758651
TCAACGC55450.040.7570654
CAACGCA57000.039.612835
TATCAAC59800.038.2988932
ATCAACG59050.038.2785343
AACGCAG60850.037.1168636
GTACATG102150.035.9131551
TACATGG105150.034.4298822
ACATGGG103950.033.9077453
CATGGGG91350.031.3979854
ACGCAGA75250.030.1724857
CGCAGAG78500.029.0737868
ATGGGGG50950.028.1802225
GCAGAGT89000.025.6443259
AGAGTAC79100.022.0359610-11
GAGTACA72450.021.1597981
CCTATAC5650.021.1114233
TATACCG2306.07579E-620.7220955
ATGGGGA38050.020.6676375
Produced by FastQC (version 0.11.5)