##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921621_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2155415
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734011779634084	33.0	31.0	34.0	30.0	34.0
2	31.919873435046153	34.0	31.0	34.0	30.0	34.0
3	32.006353300872455	34.0	31.0	34.0	30.0	34.0
4	35.39747102066191	37.0	35.0	37.0	33.0	37.0
5	35.24306224091416	37.0	35.0	37.0	33.0	37.0
6	35.26273362670298	37.0	35.0	37.0	33.0	37.0
7	35.15460874124009	37.0	35.0	37.0	33.0	37.0
8	35.190350814112364	37.0	36.0	37.0	33.0	37.0
9	36.912692451337676	39.0	38.0	39.0	34.0	39.0
10-11	36.874837096336435	39.0	38.0	39.0	34.0	39.0
12-13	36.79998584959277	39.0	38.0	39.0	33.0	39.0
14-15	38.247498741541655	41.0	39.0	41.0	34.0	41.0
16-17	38.24883955062018	41.0	39.0	41.0	34.0	41.0
18-19	38.25082965461408	41.0	39.0	41.0	34.0	41.0
20-21	38.185824075642046	41.0	39.0	41.0	34.0	41.0
22-23	38.190044840552744	41.0	39.0	41.0	34.0	41.0
24-25	38.1512411762932	41.0	39.0	41.0	34.0	41.0
26-27	38.08663760807083	41.0	39.0	41.0	34.0	41.0
28-29	38.01932876035474	41.0	39.0	41.0	33.0	41.0
30-31	37.91801880380344	41.0	38.5	41.0	33.0	41.0
32-33	37.813457269249774	41.0	38.0	41.0	33.0	41.0
34-35	37.68802318810995	41.0	38.0	41.0	33.0	41.0
36-37	37.617121992748494	41.0	38.0	41.0	33.0	41.0
38-39	37.53764240297112	40.0	38.0	41.0	32.5	41.0
40-41	37.42676955481891	40.0	38.0	41.0	32.0	41.0
42-43	37.278915197305395	40.0	38.0	41.0	32.0	41.0
44-45	37.140311494538174	40.0	37.5	41.0	31.5	41.0
46-47	36.962129798669864	40.0	37.0	41.0	31.0	41.0
48-49	36.78843842137129	40.0	37.0	41.0	31.0	41.0
50-51	36.551957975610264	40.0	36.0	41.0	30.0	41.0
52-53	36.42832865132701	40.0	36.0	41.0	30.0	41.0
54-55	36.29911594750895	40.0	35.5	41.0	30.0	41.0
56-57	36.099536516169735	39.0	35.0	41.0	30.0	41.0
58-59	35.905598457837584	39.0	35.0	41.0	30.0	41.0
60-61	35.65102938413253	39.0	35.0	41.0	29.0	41.0
62-63	35.301280727841274	38.0	35.0	41.0	29.0	41.0
64-65	35.010481972149215	37.0	35.0	40.0	29.0	41.0
66-67	34.70244662860749	37.0	35.0	39.5	29.0	41.0
68-69	34.33131670699146	36.0	35.0	39.0	28.0	41.0
70-71	33.953825829364646	35.5	35.0	39.0	28.0	41.0
72-73	33.63008886919688	35.0	34.0	37.5	28.0	40.0
74-75	33.306659970353735	35.0	34.0	37.0	27.5	39.0
76-77	32.98350387280408	35.0	34.0	36.5	27.0	39.0
78-79	32.72304614192626	35.0	34.0	36.0	27.0	38.0
80-81	32.47420427156719	35.0	34.0	36.0	26.0	37.0
82-83	32.2517248418518	35.0	34.0	35.0	26.0	37.0
84-85	32.04603498630194	35.0	34.0	35.0	26.0	36.0
86-87	31.922732049280533	35.0	34.0	35.0	26.0	36.0
88-89	31.82478548214613	35.0	34.0	35.0	25.5	36.0
90-91	31.719747937172194	35.0	34.0	35.0	25.0	36.0
92-93	31.642940454622426	35.0	34.0	35.0	25.0	35.0
94-95	31.579416956827338	35.0	33.0	35.0	25.0	35.0
96-97	31.513757907409943	35.0	33.0	35.0	25.0	35.0
98-99	31.439522319367732	35.0	33.0	35.0	24.5	35.0
100-101	31.372336185838922	35.0	33.0	35.0	24.0	35.0
102-103	30.67420960696664	34.0	31.5	35.0	23.0	35.0
104-105	31.198570112948087	35.0	33.0	35.0	24.0	35.0
106-107	31.29745315867246	35.0	33.0	35.0	24.0	35.0
108-109	31.28747294604519	35.0	33.0	35.0	24.0	35.0
110-111	31.2188880099656	35.0	33.0	35.0	23.0	35.0
112-113	31.180735264438635	35.0	33.0	35.0	23.0	35.0
114-115	31.052141466956478	35.0	33.0	35.0	20.5	35.0
116-117	31.235319648420372	35.0	33.5	35.0	23.5	35.0
118-119	31.222248383721926	35.0	34.0	35.0	23.0	35.0
120-121	31.143213487889803	35.0	34.0	35.0	21.5	35.0
122-123	31.008705748080995	35.0	33.0	35.0	19.0	35.0
124-125	30.36431012125275	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34991.0
3	9405.0
4	5005.0
5	3992.0
6	4083.0
7	4581.0
8	4959.0
9	5298.0
10	5760.0
11	6121.0
12	6434.0
13	7820.0
14	9847.0
15	8853.0
16	5539.0
17	5068.0
18	4750.0
19	4377.0
20	4349.0
21	4401.0
22	4899.0
23	6077.0
24	7843.0
25	10208.0
26	12448.0
27	14731.0
28	17951.0
29	22019.0
30	26892.0
31	33496.0
32	43570.0
33	59402.0
34	91375.0
35	163061.0
36	385404.0
37	815334.0
38	294680.0
39	392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.8351053388482	17.71870938042223	10.856590760697813	27.589594520031763
2	15.916595410631942	19.537685588526905	36.92029175460581	27.62542724623534
3	18.7055504916712	23.055545757815963	28.410371412712347	29.828532337800485
4	13.292252212060376	14.591698267529186	34.103557885344635	38.01249163506581
5	14.652848933042279	37.2679719635883	32.94477918355831	15.134399919811111
6	35.5131947246172	33.7295240465215	16.056425258214173	14.700855970647128
7	30.98604690457037	31.1861593314546	19.18280230739701	18.644991456578015
8	28.529919742746053	34.589386510784436	18.172273441862345	18.708420304607166
9	27.89506098639183	14.90248349972455	18.01656965991402	39.1858858539696
10-11	27.076648245535846	24.739397636450068	26.276503587663786	21.907450530350296
12-13	26.832350179822512	21.928969238107285	26.914909543300723	24.32377103876948
14-15	24.129188505281306	23.383791645399622	23.882572025358442	28.604447823960633
16-17	24.579990739569542	26.665629993582684	24.56717931697943	24.187199949868347
18-19	24.631392372281347	25.89478882322405	26.131372531273954	23.342446273220652
20-21	25.18237488294131	24.881688355201305	26.060906714087228	23.875030047770153
22-23	24.9999304059283	24.41884310526892	25.973621063063362	24.607605425739415
24-25	24.568636139033043	24.86478774564275	25.95847938700376	24.608096728320454
26-27	24.42139166648108	25.064722847025813	26.062452211804704	24.4514332746884
28-29	24.499248600318005	25.356201494819075	25.89547401743878	24.249075887424134
30-31	24.883801853046112	25.046465340057622	25.820207203403594	24.24952560349267
32-33	24.513547905146684	25.032195051787	25.911199423725396	24.543057619340928
34-35	24.631516775683636	25.110633121176324	26.041839687990937	24.216010415149107
36-37	24.657764646193055	25.135985906229752	25.9540240391207	24.252225408456493
38-39	24.72318003574325	25.231352174937783	25.87734275104251	24.168125038276457
40-41	24.69960194372279	25.10589354807355	25.85526192167995	24.33924258652371
42-43	24.543628005121228	24.999657826845535	25.82829681514507	24.628417352888164
44-45	24.459754393323298	25.079617095590624	25.903394212264004	24.557234298822074
46-47	24.56028387550567	25.07058146132971	25.779435079206664	24.58969958395795
48-49	24.477033309570995	25.279841549357034	25.67058625392421	24.57253888714776
50-51	24.56055984442683	25.370067382409033	25.60570979331066	24.463662979853478
52-53	24.695178177028517	25.327265468543285	25.532076783017153	24.44547957141104
54-55	24.37035710424816	25.03951869546316	25.726589551660574	24.863534648628107
56-57	24.487896559315338	25.243626529559833	25.532250517827435	24.736226393297393
58-59	24.494734156073118	25.235455135937645	25.652593486127866	24.61721722186137
60-61	24.722605237420968	25.170431453476176	25.666485108183494	24.44047820091936
62-63	24.530695178680663	25.254785026692318	25.701153222227664	24.51336657239936
64-65	24.37753286490073	25.03640830188154	25.73506726082912	24.85099157238861
66-67	24.378712143760048	25.30745127860249	25.59721974452772	24.716616833109743
68-69	24.40966307653728	25.23324551516064	25.616167389772805	24.74092401852927
70-71	24.328215216095277	25.10435345397522	25.739474764720484	24.82795656520902
72-73	24.506365854716588	25.3690033690326	25.453350973024776	24.67127980322604
74-75	24.304694633537974	25.38871402377011	25.52788181015092	24.778709532540997
76-77	24.329167637105627	25.123481591662173	25.577554234005852	24.969796537226348
78-79	24.219817988267202	25.156414520575083	25.683970640395415	24.939796850762303
80-81	24.329421480318175	25.43600652310576	25.3634450906206	24.871126905955464
82-83	24.3926718356276	25.439741363271303	25.353094532967784	24.814492268133314
84-85	24.319534931734854	25.462510714455554	25.283377776334365	24.93457657747523
86-87	24.12164246792381	25.500356079919644	25.447906783612435	24.930094668544108
88-89	24.147238871009986	25.257499145748426	25.435262345124134	25.15999963811746
90-91	24.18263431381646	25.206643778417	25.505984967987384	25.10473693977916
92-93	24.15003607193974	25.385992952633252	25.533481951271565	24.93048902415544
94-95	24.18297454379778	25.17248946690304	25.53287193860484	25.111664050694344
96-97	23.987641198541144	25.213615930689016	25.553160522913053	25.24558234785679
98-99	23.966003510254946	25.247251204997312	25.38683093014333	25.399914354604412
100-101	23.945365509658235	25.248084475611428	25.53413147816082	25.272418536569525
102-103	23.84168133147319	25.20266662088989	25.599486310347054	25.35616573728987
104-105	23.95065451432787	25.298608388639778	25.4803831280751	25.270353968957256
106-107	23.883474200294376	25.23050799096688	25.476981677436118	25.40903613130262
108-109	23.876785631021747	25.192966111780112	25.55158278259534	25.3786654746028
110-111	23.743970121728438	25.481711737312462	25.410750842356162	25.363567298602934
112-113	23.73024275637326	25.492365264738247	25.417136027822057	25.360255951066428
114-115	23.667020040224273	25.55848873650782	25.36539367128836	25.40909755197955
116-117	23.486550362025337	25.451615357705904	25.453401590628822	25.608432689639944
118-119	23.639081181309155	25.425191076456333	25.415099929037194	25.520627813197315
120-121	23.482931133914242	25.524379487945616	25.471627206073055	25.52106217206709
122-123	23.41642274318	25.64216946453749	25.253190955321752	25.688216836960752
124-125	23.278921665730405	25.568475401190348	25.325263583971008	25.827339349108236
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	15.5
2	25.5
3	62.5
4	76.0
5	85.5
6	175.5
7	414.0
8	691.0
9	760.0
10	623.0
11	520.5
12	525.5
13	551.0
14	583.0
15	647.5
16	700.0
17	713.5
18	795.5
19	887.0
20	912.0
21	1010.0
22	1166.5
23	1310.5
24	1549.5
25	1901.5
26	2418.5
27	3292.0
28	4486.0
29	5968.5
30	7822.0
31	10058.0
32	13024.0
33	16166.0
34	19371.5
35	22985.0
36	27489.5
37	32039.5
38	36568.0
39	41477.0
40	46510.5
41	51463.0
42	56091.0
43	63003.0
44	70449.5
45	76842.5
46	81922.5
47	86627.0
48	93284.5
49	98128.5
50	99989.5
51	100656.0
52	99853.0
53	98542.0
54	97854.5
55	94883.0
56	88247.5
57	80780.5
58	73997.5
59	66883.5
60	57490.0
61	47707.5
62	39345.0
63	30400.0
64	22453.0
65	17107.0
66	12963.5
67	10141.5
68	8015.5
69	6061.5
70	4621.0
71	3550.5
72	2680.5
73	2088.5
74	1628.0
75	1233.5
76	872.5
77	493.0
78	280.0
79	156.0
80	90.0
81	61.0
82	35.0
83	15.5
84	7.5
85	6.5
86	7.0
87	5.5
88	2.0
89	3.0
90	3.5
91	1.0
92	0.5
93	1.5
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023754126235550926
2	0.001948580667760037
3	0.033543424352154926
4	0.1658613306486222
5	0.023336573235316634
6	0.06894263981646226
7	0.05224051980709051
8	0.04491014491408847
9	0.03498166246407305
10-11	0.06342166125780882
12-13	0.05602169419810107
14-15	0.05848061742170301
16-17	0.050454320861643816
18-19	0.03648949274269688
20-21	0.023800521013354736
22-23	0.002737291890424814
24-25	0.0046394777803810405
26-27	0.003294029224070538
28-29	0.004825056891596282
30-31	0.002087765001171468
32-33	0.008930994727233504
34-35	0.004314714335754368
36-37	0.00633288717022012
38-39	0.0019949754455638475
40-41	0.003085252723953392
42-43	0.003410016168580065
44-45	0.00459308300257723
46-47	0.004825056891596282
48-49	0.0020181728344657525
50-51	0.0014150407230162172
52-53	7.655138337628717E-4
54-55	0.004917846447203902
56-57	7.191190559590612E-4
58-59	6.959216670571561E-4
60-61	1.391843334114312E-4
62-63	9.046981671743029E-4
64-65	0.0
66-67	0.003410016168580065
68-69	6.727242781552509E-4
70-71	0.0
72-73	0.0022501467234848044
74-75	0.0047786621137924715
76-77	0.0015310276675257434
78-79	2.087765001171468E-4
80-81	0.0
82-83	0.005289004669634386
84-85	0.0015078302786238381
86-87	0.0
88-89	9.046981671743029E-4
90-91	6.959216670571561E-4
92-93	0.0
94-95	0.0030620553350514867
96-97	0.0012990537785066913
98-99	7.423164448609664E-4
100-101	0.0
102-103	0.0012990537785066913
104-105	0.0
106-107	5.799347225476301E-4
108-109	0.002296541501288615
110-111	1.15986944509526E-4
112-113	1.391843334114312E-4
114-115	0.0
116-117	0.0019021858899562266
118-119	0.0028068840571305294
120-121	0.0026908971126210035
122-123	0.0012062642228990706
124-125	0.10919010956126779
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2155415.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.77444240534499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85615377935555	44.9809587242706
2	11.781522312619973	13.613417645954993
3	3.9840322491566367	6.905257251598115
4	1.9054632487710883	4.4034830688650715
5	1.1081349709157589	3.2010940027260584
6	0.7206880140618155	2.4982408896382093
7	0.5037708693178728	2.0373556752427215
8	0.36863378585140444	1.7038089143468997
9	0.2836779676500928	1.4750402763299044
>10	1.4002697180855819	14.134161886588863
>50	0.06554759071814877	2.5633109351597456
>100	0.02105779157140295	1.946593635306631
>500	8.059245574070768E-4	0.2823975712749224
>1k	2.4177736722206587E-4	0.25487952269721625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2349	0.10898133306115063	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.63947778038104E-5
2	0.0	0.0	0.0	0.0	9.27895556076208E-5
3	0.0	0.0	0.0	0.0	9.27895556076208E-5
4	0.0	0.0	0.0	0.0	9.27895556076208E-5
5	0.0	0.0	0.0	0.0	9.27895556076208E-5
6	0.0	0.0	0.0	0.0	1.391843334114312E-4
7	0.0	0.0	0.0	0.0	1.391843334114312E-4
8	0.0	0.0	0.0	0.0	1.391843334114312E-4
9	0.0	0.0	0.0	0.0	2.3197388901905202E-4
10-11	0.0	0.0	0.0	0.0	2.3197388901905202E-4
12-13	0.0	0.0	0.0	0.0	2.3197388901905202E-4
14-15	0.0	0.0	0.0	0.0	2.783686668228624E-4
16-17	0.0	0.0	0.0	2.31973889019052E-5	3.9435561133238845E-4
18-19	0.0	0.0	0.0	4.63947778038104E-5	4.1755300023429363E-4
20-21	0.0	0.0	0.0	4.63947778038104E-5	4.407503891361988E-4
22-23	0.0	0.0	0.0	6.95921667057156E-5	4.6394777803810403E-4
24-25	0.0	0.0	0.0	3.711582224304832E-4	4.6394777803810403E-4
26-27	0.0	0.0	0.0	6.031321114495352E-4	5.103425558419144E-4
28-29	0.0	0.0	0.0	0.0037579770021086427	5.103425558419144E-4
30-31	0.0	0.0	0.0	0.01329210384079168	5.103425558419144E-4
32-33	0.0	0.0	0.0	0.031014908961847255	5.103425558419144E-4
34-35	0.0	0.0	0.0	0.05279725714073624	5.567373336457248E-4
36-37	0.0	0.0	0.0	0.08664224754861592	5.567373336457248E-4
38-39	0.0	0.0	0.0	0.14391660074741985	5.567373336457248E-4
40-41	0.0	0.0	0.0	0.20951881656200777	5.799347225476301E-4
42-43	0.0	0.0	0.0	0.2689041321508851	6.031321114495352E-4
44-45	0.0	0.0	0.0	0.33471512446559015	6.495268892533455E-4
46-47	0.0	0.0	0.0	0.41068657311932966	6.95921667057156E-4
48-49	0.0	0.0	0.0	0.4900448405527474	6.95921667057156E-4
50-51	0.0	0.0	0.0	0.5732074797660776	6.95921667057156E-4
52-53	2.31973889019052E-5	0.0	0.0	0.6608472150374753	7.423164448609664E-4
54-55	4.63947778038104E-5	0.0	0.0	0.7506907022545541	7.423164448609664E-4
56-57	4.63947778038104E-5	0.0	0.0	0.8506250536439619	7.423164448609664E-4
58-59	4.63947778038104E-5	0.0	0.0	0.9586784911490362	7.423164448609664E-4
60-61	4.63947778038104E-5	0.0	0.0	1.0735519609912707	7.423164448609664E-4
62-63	4.63947778038104E-5	0.0	0.0	1.1943871597812952	7.887112226647768E-4
64-65	4.63947778038104E-5	0.0	0.0	1.309654985234862	7.887112226647768E-4
66-67	4.63947778038104E-5	0.0	0.0	1.4400243108635693	8.583033893704924E-4
68-69	4.63947778038104E-5	0.0	0.0	1.587930862502117	8.815007782723976E-4
70-71	4.63947778038104E-5	0.0	0.0	1.7490831232036523	9.510929449781133E-4
72-73	4.63947778038104E-5	0.0	0.0	1.905735090458218	0.0010902772783895444
74-75	4.63947778038104E-5	0.0	0.0	2.0703205647172354	0.001136672056193355
76-77	4.63947778038104E-5	0.0	0.0	2.2548557934318914	0.0011598694450952601
78-79	4.63947778038104E-5	0.0	0.0	2.4643050178271935	0.0012062642228990704
80-81	4.63947778038104E-5	0.0	0.0	2.678880865169816	0.0012526590007028808
82-83	4.63947778038104E-5	0.0	0.0	2.9025036941841824	0.0013454485563105017
84-85	4.63947778038104E-5	0.0	0.0	3.1277271430327804	0.0013454485563105017
86-87	4.63947778038104E-5	0.0	0.0	3.378096561451043	0.001391843334114312
88-89	4.63947778038104E-5	0.0	0.0	3.6585993880528807	0.0014614355008200277
90-91	4.63947778038104E-5	0.0	0.0	3.9613949053894495	0.001484632889721933
92-93	4.63947778038104E-5	0.0	0.0	4.270407322951728	0.001484632889721933
94-95	4.63947778038104E-5	0.0	0.0	4.582528190626864	0.0015078302786238381
96-97	4.63947778038104E-5	0.0	0.0	4.914065272812892	0.0016238172231333643
98-99	4.63947778038104E-5	0.0	0.0	5.283854849298162	0.001716606778740985
100-101	4.63947778038104E-5	0.0	0.0	5.682130819354974	0.001716606778740985
102-103	4.63947778038104E-5	0.0	0.0	6.0949283548643765	0.001716606778740985
104-105	4.63947778038104E-5	0.0	0.0	6.518675057935479	0.001716606778740985
106-107	4.63947778038104E-5	0.0	0.0	6.9719752344676085	0.001716606778740985
108-109	4.63947778038104E-5	0.0	0.0	7.450885328347441	0.001716606778740985
110-111	4.63947778038104E-5	0.0	0.0	7.963756399579664	0.001716606778740985
112-113	4.63947778038104E-5	0.0	0.0	8.485488873372413	0.001716606778740985
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4585	0.0	50.739014	1
GGTATCA	2625	0.0	43.175865	1
TCAACGC	5545	0.0	40.757065	4
CAACGCA	5700	0.0	39.61283	5
TATCAAC	5980	0.0	38.298893	2
ATCAACG	5905	0.0	38.278534	3
AACGCAG	6085	0.0	37.116863	6
GTACATG	10215	0.0	35.913155	1
TACATGG	10515	0.0	34.429882	2
ACATGGG	10395	0.0	33.907745	3
CATGGGG	9135	0.0	31.397985	4
ACGCAGA	7525	0.0	30.172485	7
CGCAGAG	7850	0.0	29.073786	8
ATGGGGG	5095	0.0	28.180222	5
GCAGAGT	8900	0.0	25.644325	9
AGAGTAC	7910	0.0	22.03596	10-11
GAGTACA	7245	0.0	21.159798	1
CCTATAC	565	0.0	21.111423	3
TATACCG	230	6.07579E-6	20.722095	5
ATGGGGA	3805	0.0	20.667637	5
>>END_MODULE