FastQCFastQC Report
Mon 15 Aug 2016
SRR2921620_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921620_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259011
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG2500.085.7092362
TACGGTC1350.061.719855
GTACGGT1700.056.0191044
AGTACGG3450.051.7567863
GTATCAA25450.038.825761
GTACATG65250.038.7710951
TACATGG68350.036.922612
GGTATCA17700.036.320361
ACATGGG70050.035.4317863
TACGGTA700.002785510634.0088965
CATGGGG58650.032.9822654
TCAACGC29250.032.151144
CAACGCA31000.030.5257245
TATTACG1004.4300128E-429.7601492
AACGCAG32000.029.568276
ATGGGGG37900.029.5222325
TATCAAC33300.028.9558222
TTACGGT1454.0565083E-628.7339384
TAGGACG1454.0565083E-628.7339382
ATCAACG32800.028.4898993
Produced by FastQC (version 0.11.5)