##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921620_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259011
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.86470412093302	31.0	31.0	34.0	26.0	34.0
2	29.953945596980486	31.0	31.0	34.0	26.0	34.0
3	29.947551689381587	31.0	31.0	34.0	25.0	34.0
4	33.372456634612405	37.0	35.0	37.0	28.0	37.0
5	33.53722882484744	37.0	35.0	37.0	28.0	37.0
6	33.40259298767048	37.0	35.0	37.0	27.0	37.0
7	33.421617444168476	37.0	35.0	37.0	27.0	37.0
8	33.39151762772526	37.0	35.0	37.0	27.0	37.0
9	34.98098586906707	39.0	37.0	39.0	27.0	39.0
10-11	35.04486458021415	39.0	37.0	39.0	27.0	39.0
12-13	34.990679986116085	39.0	37.0	39.0	27.0	39.0
14-15	36.26657829042002	40.0	38.0	41.0	27.0	41.0
16-17	36.23299558145243	40.0	38.0	41.0	27.0	41.0
18-19	36.180316931305605	40.0	38.0	41.0	27.0	41.0
20-21	36.22962587300667	40.0	38.0	41.0	27.0	41.0
22-23	36.15741760794782	40.0	38.0	41.0	27.0	41.0
24-25	36.145075777733474	40.0	38.0	41.0	27.0	41.0
26-27	36.0988760225288	40.0	37.0	41.0	27.0	41.0
28-29	36.043069520441044	40.0	37.0	41.0	27.0	41.0
30-31	35.93966534049345	40.0	37.0	41.0	26.0	41.0
32-33	35.83927821123088	40.0	37.0	41.0	25.5	41.0
34-35	35.70365667972718	40.0	37.0	41.0	25.0	41.0
36-37	35.59414969368814	40.0	36.5	41.0	24.5	41.0
38-39	35.47446487759043	40.0	36.0	41.0	24.0	41.0
40-41	35.35813785582493	40.0	36.0	41.0	23.5	41.0
42-43	35.108671012405765	40.0	35.0	41.0	22.5	41.0
44-45	34.75000258139127	40.0	35.0	41.0	20.5	41.0
46-47	34.49659256352804	39.0	35.0	41.0	18.5	41.0
48-49	34.29287988746722	39.0	35.0	41.0	16.0	41.0
50-51	34.0874118653451	39.0	34.0	41.0	15.0	41.0
52-53	33.883628896014415	38.5	34.0	40.0	13.5	41.0
54-55	33.64145110725799	38.0	34.0	40.0	10.0	41.0
56-57	33.38618248768279	38.0	33.5	40.0	8.0	41.0
58-59	33.37499394365895	38.0	34.0	40.0	8.0	41.0
60-61	33.158653498658865	37.0	33.0	40.0	6.5	41.0
62-63	32.84609943836869	37.0	33.0	40.0	2.0	41.0
64-65	32.550178671989364	36.0	33.0	39.0	2.0	41.0
66-67	32.223232759682006	36.0	33.0	39.0	2.0	41.0
68-69	31.88483103007043	35.0	32.5	39.0	2.0	40.5
70-71	31.52728371713988	35.0	32.0	37.5	2.0	40.0
72-73	31.17901710151857	35.0	32.0	37.0	2.0	39.0
74-75	30.817813347143115	35.0	31.5	36.5	2.0	39.0
76-77	30.52961610343357	35.0	31.0	36.0	2.0	38.5
78-79	30.259510441131965	35.0	31.0	36.0	2.0	37.0
80-81	30.0080626777685	35.0	31.0	35.0	2.0	37.0
82-83	29.786316005181845	35.0	31.0	35.0	2.0	36.5
84-85	29.57392191172277	35.0	31.0	35.0	2.0	36.0
86-87	29.401966305298366	35.0	31.0	35.0	2.0	36.0
88-89	29.23394950480973	34.0	30.0	35.0	2.0	35.5
90-91	29.047708081978634	34.0	30.0	35.0	2.0	35.0
92-93	28.962240202825868	34.0	30.0	35.0	2.0	35.0
94-95	28.83483106978414	34.0	30.0	35.0	2.0	35.0
96-97	28.73184547235886	34.0	30.0	35.0	2.0	35.0
98-99	28.629276471770304	34.0	29.5	35.0	2.0	35.0
100-101	28.50582639865736	34.0	29.0	35.0	2.0	35.0
102-103	27.627004847455662	33.0	28.0	34.5	2.0	35.0
104-105	28.526693968519737	34.0	29.5	35.0	2.0	35.0
106-107	28.790826291430335	34.5	30.5	35.0	2.0	35.0
108-109	28.779010270760146	35.0	31.0	35.0	2.0	35.0
110-111	28.703754772595314	35.0	30.0	35.0	2.0	35.0
112-113	28.60735370858555	35.0	30.0	35.0	2.0	35.0
114-115	28.477694793770667	35.0	30.0	35.0	2.0	35.0
116-117	28.337911662408032	34.5	30.0	35.0	2.0	35.0
118-119	28.03870617492619	34.0	29.0	35.0	2.0	35.0
120-121	27.897441722113626	34.0	29.0	35.0	2.0	35.0
122-123	27.651339027220573	34.0	29.0	35.0	2.0	35.0
124-125	26.886954522240075	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61524.0
3	9956.0
4	5117.0
5	4758.0
6	4994.0
7	5321.0
8	5019.0
9	5090.0
10	5334.0
11	5696.0
12	5757.0
13	5841.0
14	5907.0
15	5319.0
16	4330.0
17	3970.0
18	3623.0
19	3349.0
20	3393.0
21	3593.0
22	4339.0
23	5606.0
24	7870.0
25	10166.0
26	11352.0
27	12438.0
28	15022.0
29	17751.0
30	21619.0
31	26868.0
32	35001.0
33	48117.0
34	73140.0
35	121050.0
36	240491.0
37	360139.0
38	90105.0
39	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21165242303313	17.57405113725291	11.726070973733824	25.488225465980136
2	16.760467497515634	19.637405838396724	38.33190480691857	25.270221857169073
3	19.87687566030392	24.274956509305817	28.017857001008828	27.83031082938144
4	14.476838678620283	15.003900335698955	33.83592852614973	36.68333245953104
5	14.7087235185144	37.64978503404819	32.93713715558578	14.704354291851631
6	34.41568560107361	34.7278139250892	16.145286751003326	14.711213722833866
7	30.067672549105318	31.946926919210185	18.993773171788135	18.991627359896366
8	28.62643099970915	34.556328503336815	18.431069639999173	18.386170856954863
9	28.061160038612336	16.04215577615966	17.889509917253598	38.007174267974406
10-11	26.826508239620907	25.606637470189636	25.866020434355207	21.700833855834247
12-13	26.40635916509148	22.976825175786207	26.36127814038986	24.255537518732456
14-15	24.18195577430715	24.329930898596324	23.463809654334085	28.024303672762446
16-17	24.860178282125624	27.31141846242592	24.08747408759327	23.74092916785519
18-19	24.757215785632354	26.647124472624974	25.39632340291631	23.199336338826367
20-21	25.31737793408486	25.498526775268576	25.481365306103804	23.70272998454276
22-23	25.593280740291362	24.91975766995105	25.18999325801539	24.296968331742193
24-25	25.13115628076303	25.315923163037407	25.165757862027487	24.387162694172076
26-27	24.62518624471065	25.57895782426446	25.47156167431512	24.324294256709774
28-29	24.79140940493275	25.76058090692807	25.339704683395237	24.108305004743947
30-31	25.163068176401232	25.52948495085312	25.230241936765303	24.077204935980347
32-33	24.70229677206118	25.652770141645398	25.236168360034196	24.40876472625922
34-35	24.81693822981354	25.78562602556309	25.383863546968303	24.013572197655062
36-37	24.908708607088357	25.72443304808	25.319629099176172	24.047229245655465
38-39	24.862542095366113	25.86949423594378	25.222780713231945	24.04518295545816
40-41	24.820208625933425	25.661704963755668	25.313346139864002	24.204740270446912
42-43	24.709587774324557	25.599295479245715	25.214383091501897	24.47673365492783
44-45	24.554805062748127	25.548090347770998	25.481042498723987	24.416062090756892
46-47	24.46756406014207	25.57138425201167	25.539168972912552	24.42188271493371
48-49	24.47593978869155	25.874916774852736	25.33198690008374	24.31715653637197
50-51	24.503017923214372	25.860984056689446	25.404581268616223	24.231416751479962
52-53	24.65846913132979	25.665523397297623	25.274833534736242	24.40117393663634
54-55	24.25429428770953	25.5320340635271	25.446907024430626	24.766764624332748
56-57	24.48027667200694	25.69969440073665	25.161548915148437	24.658480012107976
58-59	24.499972592531982	25.713130401555155	25.30273335870179	24.484163647211073
60-61	24.77558319767825	25.69215869936371	25.432408018704912	24.099850084253127
62-63	24.507994163817497	25.691000910066304	25.378813608808137	24.422191317308062
64-65	24.394520365113028	25.4381884648339	25.449072420810282	24.71821874924279
66-67	24.49446165226017	25.67661674347616	25.27276605938107	24.556155544882603
68-69	24.331699922778448	25.74937475967573	25.372403306226342	24.54652201131947
70-71	24.285840861619786	25.56208358017054	25.54241606839685	24.609659489812824
72-73	24.466116294709444	25.848070805009353	25.250481737663797	24.435331162617395
74-75	24.36415702846975	25.821682765632943	25.334265378749365	24.47989482714794
76-77	24.32228676381801	25.526377670020146	25.39025510171657	24.76108046444528
78-79	24.27153264954277	25.558009645101578	25.49862156085392	24.671836144501736
80-81	24.41318227713955	25.827725394647675	25.31186918137468	24.44722314683809
82-83	24.48457508950742	25.658291189800224	25.439890299036026	24.417243421656327
84-85	24.486067205175534	25.737848101416656	25.13145963387911	24.644625059528703
86-87	24.29524512401382	25.84223023885997	25.34162290043225	24.520901736693965
88-89	24.265314285918574	25.583202280732348	25.43205649237697	24.71942694097211
90-91	24.440776733738108	25.458149368511577	25.43487198431756	24.666201913432758
92-93	24.509047350295436	25.475641718653	25.451771758699728	24.563539172351835
94-95	24.522920395247436	25.378974736606303	25.426557583585748	24.671547284560518
96-97	24.29644611498758	25.353718267039664	25.521496370964382	24.828339247008373
98-99	24.195684767606963	25.41673865340125	25.410622263262933	24.976954315728854
100-101	24.18073481656594	25.630802813947724	25.531587008051655	24.656875361434683
102-103	23.96485953544528	25.59582508404838	25.62485727164953	24.8144581088568
104-105	23.9852177228462	25.684901583872144	25.521430727437366	24.808449965844282
106-107	23.98078468112078	25.598026363080727	25.613038330955114	24.80815062484338
108-109	23.88646423498683	25.66776398364193	25.60333897879615	24.842432802575093
110-111	23.73588946042377	26.094847607761157	25.559060134012395	24.61020279780268
112-113	23.678803023419107	26.096251544911247	25.645685527557404	24.579259904112245
114-115	23.711700547333635	26.206596601606254	25.48274985502411	24.598952996036
116-117	23.591504964360425	26.112905192412406	25.57629593855385	24.719293904673318
118-119	23.6688612850127	25.92559698172603	25.646128644648265	24.75941308861301
120-121	23.644573648028786	26.13735558918026	25.645296446769052	24.572774316021906
122-123	23.58667256554225	26.198553292819604	25.5703945283428	24.644379613295346
124-125	23.465707710745065	26.17966399357438	25.618378359426792	24.73624993625376
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	942.0
1	752.0
2	475.0
3	334.5
4	254.0
5	214.5
6	212.5
7	244.0
8	280.0
9	301.5
10	301.5
11	300.5
12	335.5
13	367.0
14	386.0
15	434.5
16	461.0
17	516.5
18	592.5
19	624.5
20	692.5
21	782.5
22	892.5
23	1014.5
24	1207.0
25	1461.0
26	1837.5
27	2489.0
28	3173.0
29	4054.0
30	5437.0
31	6951.5
32	8768.0
33	10642.5
34	12487.5
35	14487.5
36	16932.5
37	19656.0
38	22208.5
39	24996.5
40	27355.0
41	29651.0
42	32341.5
43	35640.0
44	39452.0
45	42916.0
46	46155.0
47	49536.5
48	52629.0
49	55072.0
50	57167.5
51	58103.5
52	57254.0
53	56184.5
54	55814.0
55	54435.5
56	51162.5
57	46770.0
58	42462.0
59	38023.0
60	32866.0
61	28026.5
62	23449.5
63	18258.5
64	13617.5
65	10561.0
66	8224.0
67	6364.5
68	4962.0
69	3869.5
70	3070.5
71	2333.0
72	1732.0
73	1387.5
74	1103.0
75	842.0
76	615.5
77	404.0
78	280.0
79	189.5
80	132.0
81	103.0
82	88.5
83	69.5
84	50.5
85	37.0
86	31.0
87	32.5
88	26.0
89	22.0
90	18.5
91	14.0
92	10.0
93	11.5
94	15.0
95	12.5
96	9.5
97	6.0
98	3.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0254167755484265
2	0.01096098445525893
3	0.00961071825424877
4	0.01151697642038076
5	0.016441476682888395
6	0.09491577118865523
7	0.05925285799726929
8	0.04964213974302051
9	0.02748188856173616
10-11	0.050833551096853
12-13	0.013224666027540665
14-15	0.028077594238652404
16-17	0.03526577607344177
18-19	0.04269224017899764
20-21	0.02998385240478439
22-23	0.03808544961084534
24-25	0.031612114588355465
26-27	0.046544470223056036
28-29	0.044876494327690544
30-31	0.026608186902258992
32-33	0.029785283845812305
34-35	0.04416164751539105
36-37	0.010325565066548267
38-39	0.0208099849802742
40-41	0.04277166760258647
42-43	0.02247796087563969
44-45	0.016560617818271645
46-47	0.02315309397614477
48-49	0.029944138692989977
50-51	0.028911582186335147
52-53	0.027005324020203156
54-55	0.024145936771005176
56-57	0.026727328037642245
58-59	0.01826830742543155
60-61	0.024145936771005176
62-63	0.024741642447921423
64-65	0.02204111004590111
66-67	0.02998385240478439
68-69	0.023431089958705683
70-71	0.04690189362920578
72-73	0.0227559568582006
74-75	0.0104049924901371
76-77	0.017394605765954387
78-79	0.027084751443791993
80-81	0.01826830742543155
82-83	0.025138779565865587
84-85	0.013065811180362999
86-87	0.019062581661319876
88-89	0.023232521399733603
90-91	0.022120537469489943
92-93	0.008459020612210696
94-95	0.012231823232680255
96-97	0.016322335547505146
98-99	0.007823601223500032
100-101	0.011040411878847762
102-103	0.005043641397890884
104-105	0.00563934707480713
106-107	0.0012311250656269089
108-109	0.014535218516756408
110-111	0.001826830742543155
112-113	0.0030976695199644803
114-115	0.014376363669578741
116-117	0.006949899564022872
118-119	0.28526359181929306
120-121	0.001270838777421325
122-123	0.0011119839302436594
124-125	0.32048965418094044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1259011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.56368832889558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.14137528796871	53.85491528287226
2	9.704063492015825	12.724683886286792
3	3.2288183775612147	6.350797253311013
4	1.5100027088552133	3.96005387916685
5	0.8840267227842409	2.8980026263520466
6	0.5742233204903515	2.2588919289487754
7	0.39094887183357435	1.7942434989800864
8	0.2876276683200489	1.5086344640402107
9	0.2033552289945075	1.1999446968462368
>10	1.044680564309579	11.596560477249843
>50	0.024718695797467473	1.0781977076560698
>100	0.005917532769703957	0.6462526184398748
>500	1.2076414976058795E-4	0.039747070242560426
>1k	1.2076414976058795E-4	0.08907460960733164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.942742358883282E-5	7.942742358883282E-5
9	0.0	0.0	0.0	7.942742358883282E-5	7.942742358883282E-5
10-11	0.0	0.0	0.0	7.942742358883282E-5	7.942742358883282E-5
12-13	0.0	0.0	0.0	7.942742358883282E-5	2.7799598256091486E-4
14-15	0.0	0.0	0.0	1.1914113538324922E-4	5.957056769162461E-4
16-17	0.0	0.0	0.0	2.3828227076649845E-4	9.531290830659939E-4
18-19	0.0	0.0	0.0	3.574234061497477E-4	9.531290830659939E-4
20-21	0.0	7.942742358883282E-5	0.0	4.3685082973858054E-4	9.531290830659939E-4
22-23	0.0	7.942742358883282E-5	0.0	6.354193887106626E-4	0.0011914113538324922
24-25	0.0	7.942742358883282E-5	0.0	0.0013502662010101579	0.0015885484717766565
26-27	0.0	7.942742358883282E-5	0.0	0.0027005324020203158	0.0015885484717766565
28-29	0.0	7.942742358883282E-5	0.0	0.006711617293256374	0.0015885484717766565
30-31	0.0	7.942742358883282E-5	0.0	0.019698001050030542	0.001628262183571073
32-33	0.0	7.942742358883282E-5	0.0	0.04868901065995452	0.0017474033189543222
34-35	0.0	7.942742358883282E-5	0.0	0.08649646428823894	0.001826830742543155
36-37	0.0	7.942742358883282E-5	0.0	0.144677052067059	0.001826830742543155
38-39	0.0	7.942742358883282E-5	0.0	0.2260107338220238	0.001826830742543155
40-41	0.0	7.942742358883282E-5	0.0	0.3167168515604709	0.001826830742543155
42-43	0.0	7.942742358883282E-5	0.0	0.4050004328794585	0.0018665444543375714
44-45	0.0	7.942742358883282E-5	0.0	0.5096063497459514	0.0019856855897208204
46-47	0.0	7.942742358883282E-5	0.0	0.6269603680984519	0.0021048267251040694
48-49	0.0	7.942742358883282E-5	0.0	0.7468163502940006	0.0023033952840761517
50-51	0.0	7.942742358883282E-5	0.0	0.8579353158947778	0.0023033952840761517
52-53	0.0	7.942742358883282E-5	0.0	0.9781487214964761	0.0024622501312538173
54-55	0.0	7.942742358883282E-5	0.0	1.113651906139025	0.0027005324020203158
56-57	0.0	1.5885484717766563E-4	0.0	1.2626974665034698	0.002740246113814732
58-59	0.0	1.5885484717766563E-4	0.0	1.4186134990083485	0.0027799598256091486
60-61	0.0	1.5885484717766563E-4	0.0	1.571709857975824	0.0027799598256091486
62-63	0.0	1.5885484717766563E-4	0.0	1.7353700642806138	0.002938814672786814
64-65	0.0	1.5885484717766563E-4	0.0	1.9031604966120232	0.002978528384581231
66-67	0.0	1.5885484717766563E-4	0.0	2.0926743292949785	0.003018242096375647
68-69	0.0	1.5885484717766563E-4	0.0	2.302601009840264	0.003018242096375647
70-71	0.0	2.3828227076649845E-4	0.0	2.522217836063386	0.0030579558081700636
72-73	0.0	3.1770969435533127E-4	0.0	2.730397113289717	0.003534520349703061
74-75	0.0	3.1770969435533127E-4	0.0	2.945168866673921	0.003574234061497477
76-77	0.0	3.1770969435533127E-4	0.0	3.1945312630310614	0.003574234061497477
78-79	0.0	3.1770969435533127E-4	0.0	3.4781268789549893	0.00365366148508631
80-81	0.0	3.1770969435533127E-4	0.0	3.768990104137295	0.003812516332263975
82-83	0.0	3.1770969435533127E-4	0.0	4.063308422245715	0.0041302260266193064
84-85	0.0	3.1770969435533127E-4	0.0	4.365251773018663	0.0041302260266193064
86-87	0.0	3.1770969435533127E-4	0.0	4.6996809400394435	0.004249367162002556
88-89	0.0	3.1770969435533127E-4	3.971371179441641E-5	5.069256741998283	0.004447935720974638
90-91	0.0	3.1770969435533127E-4	7.942742358883282E-5	5.458649686142536	0.004447935720974638
92-93	0.0	3.9713711794416414E-4	7.942742358883282E-5	5.848797190810883	0.004447935720974638
94-95	0.0	3.9713711794416414E-4	7.942742358883282E-5	6.242082078710988	0.004447935720974638
96-97	0.0	4.3685082973858054E-4	7.942742358883282E-5	6.689933606616622	0.00452736314456347
98-99	0.0	4.7656454153299695E-4	7.942742358883282E-5	7.172296350071604	0.0046465042799467195
100-101	0.0	4.7656454153299695E-4	7.942742358883282E-5	7.691910555189748	0.004686217991741137
102-103	0.0	7.148468122994953E-4	7.942742358883282E-5	8.226576257077976	0.004805359127124386
104-105	0.0	7.148468122994953E-4	7.942742358883282E-5	8.768668423071759	0.004884786550713218
106-107	0.0	7.148468122994953E-4	7.942742358883282E-5	9.346582357104108	0.004924500262507635
108-109	0.0	7.148468122994953E-4	7.942742358883282E-5	9.977156672975852	0.004964213974302051
110-111	0.0	7.148468122994953E-4	7.942742358883282E-5	10.642440772955915	0.005003927686096468
112-113	0.0	7.148468122994953E-4	7.942742358883282E-5	11.319559559050715	0.005003927686096468
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	250	0.0	85.709236	2
TACGGTC	135	0.0	61.71985	5
GTACGGT	170	0.0	56.019104	4
AGTACGG	345	0.0	51.756786	3
GTATCAA	2545	0.0	38.82576	1
GTACATG	6525	0.0	38.771095	1
TACATGG	6835	0.0	36.92261	2
GGTATCA	1770	0.0	36.32036	1
ACATGGG	7005	0.0	35.431786	3
TACGGTA	70	0.0027855106	34.008896	5
CATGGGG	5865	0.0	32.982265	4
TCAACGC	2925	0.0	32.15114	4
CAACGCA	3100	0.0	30.525724	5
TATTACG	100	4.4300128E-4	29.760149	2
AACGCAG	3200	0.0	29.56827	6
ATGGGGG	3790	0.0	29.522232	5
TATCAAC	3330	0.0	28.955822	2
TTACGGT	145	4.0565083E-6	28.733938	4
TAGGACG	145	4.0565083E-6	28.733938	2
ATCAACG	3280	0.0	28.489899	3
>>END_MODULE