FastQCFastQC Report
Mon 15 Aug 2016
SRR2921620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259011
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.21794885032775727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.17474033189543223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.12335078883345736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.053.6821671
GTATCAA34000.051.079211
TCAACGC46150.036.964284
CAACGCA46400.036.4615485
TATCAAC47750.036.107622
ATCAACG48900.034.8924563
AACGCAG48950.034.8193666
GTACATG64650.034.7095831
TACATGG66150.033.8201682
CATGGGG56100.032.1093834
ATGGGGG35200.031.308615
ACATGGG69200.031.2949963
GTATAGG3050.029.3509041
ACGCAGA58600.028.779227
CGCAGAG61650.027.4510028
TATACCG1608.661667E-626.0623025
GCAGAGT67600.024.9466769
CTTACAC6250.023.8635123
ATACCGT2001.8009905E-623.8378736
GAGTACT31950.023.48110612-13
Produced by FastQC (version 0.11.5)