##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921620_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1259011
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61237590457907	33.0	31.0	34.0	30.0	34.0
2	31.79699621369472	33.0	31.0	34.0	30.0	34.0
3	31.883794502192593	34.0	31.0	34.0	30.0	34.0
4	35.266798304383364	37.0	35.0	37.0	33.0	37.0
5	35.09540663266643	37.0	35.0	37.0	33.0	37.0
6	35.122902023890184	37.0	35.0	37.0	33.0	37.0
7	35.007859343564114	37.0	35.0	37.0	33.0	37.0
8	35.04171448859462	37.0	35.0	37.0	33.0	37.0
9	36.74752325436394	39.0	38.0	39.0	33.0	39.0
10-11	36.7057130557239	39.0	38.0	39.0	33.0	39.0
12-13	36.62587936086341	39.0	38.0	39.0	33.0	39.0
14-15	38.038909509130576	41.0	39.0	41.0	33.0	41.0
16-17	38.04133164841292	41.0	39.0	41.0	33.0	41.0
18-19	38.05809361474999	41.0	39.0	41.0	33.0	41.0
20-21	37.997260151023305	41.0	39.0	41.0	33.0	41.0
22-23	37.99631695036818	41.0	39.0	41.0	33.0	41.0
24-25	37.9523348088301	41.0	39.0	41.0	33.0	41.0
26-27	37.87550227916992	41.0	39.0	41.0	33.0	41.0
28-29	37.79770828054719	41.0	38.0	41.0	33.0	41.0
30-31	37.673358294724984	41.0	38.0	41.0	33.0	41.0
32-33	37.539419036052905	41.0	38.0	41.0	32.0	41.0
34-35	37.402704980337745	40.5	38.0	41.0	32.0	41.0
36-37	37.31954685066294	40.0	38.0	41.0	32.0	41.0
38-39	37.22753931458899	40.0	38.0	41.0	31.5	41.0
40-41	37.097615906453555	40.0	38.0	41.0	31.0	41.0
42-43	36.92825559109491	40.0	37.0	41.0	31.0	41.0
44-45	36.75073251941404	40.0	37.0	41.0	30.0	41.0
46-47	36.52143388739256	40.0	37.0	41.0	30.0	41.0
48-49	36.30963788243312	40.0	36.0	41.0	29.5	41.0
50-51	36.058396233233864	40.0	36.0	41.0	28.5	41.0
52-53	35.916924077708615	40.0	35.0	41.0	28.0	41.0
54-55	35.78082002460661	39.5	35.0	41.0	28.0	41.0
56-57	35.57954100480457	39.0	35.0	41.0	28.0	41.0
58-59	35.37870121865496	39.0	35.0	41.0	28.0	41.0
60-61	35.124587473818735	38.5	35.0	41.0	28.0	41.0
62-63	34.76857589012328	37.5	35.0	40.5	26.5	41.0
64-65	34.47771623917504	37.0	35.0	40.0	27.0	41.0
66-67	34.168112113396944	36.5	35.0	39.5	26.0	41.0
68-69	33.800262269352686	36.0	35.0	39.0	26.0	41.0
70-71	33.4318981327407	35.5	34.0	39.0	26.0	41.0
72-73	33.11777180660058	35.0	34.0	37.0	26.0	39.5
74-75	32.79840843328613	35.0	34.0	37.0	26.0	39.0
76-77	32.482778943154585	35.0	34.0	36.5	25.0	39.0
78-79	32.22745512151999	35.0	34.0	36.0	25.0	38.0
80-81	31.988062852508833	35.0	34.0	36.0	24.0	37.0
82-83	31.768853488968723	35.0	34.0	35.0	24.0	37.0
84-85	31.562860054439554	35.0	33.5	35.0	23.5	36.0
86-87	31.44361606054276	35.0	33.0	35.0	23.0	36.0
88-89	31.343012888688023	35.0	33.0	35.0	23.0	36.0
90-91	31.24150742130132	35.0	33.0	35.0	22.0	35.5
92-93	31.165786081297142	35.0	33.0	35.0	20.5	35.0
94-95	31.09864131449209	35.0	33.0	35.0	20.0	35.0
96-97	31.032458810923814	35.0	33.0	35.0	20.0	35.0
98-99	30.962027734467767	35.0	33.0	35.0	19.5	35.0
100-101	30.89020826664739	35.0	33.0	35.0	19.0	35.0
102-103	30.198871177455956	34.0	31.5	35.0	18.5	35.0
104-105	30.715135133847124	35.0	33.0	35.0	18.0	35.0
106-107	30.813731968982005	35.0	33.0	35.0	18.0	35.0
108-109	30.795242853318996	35.0	33.0	35.0	18.0	35.0
110-111	30.72474068931884	35.0	33.0	35.0	17.0	35.0
112-113	30.67751512893851	35.0	33.0	35.0	16.0	35.0
114-115	30.544759736015017	35.0	33.0	35.0	10.0	35.0
116-117	30.72260369448718	35.0	33.0	35.0	8.5	35.0
118-119	30.695352145453853	35.0	33.0	35.0	2.0	35.0
120-121	30.607224639022217	35.0	33.0	35.0	2.0	35.0
122-123	30.463238605540383	35.0	33.0	35.0	2.0	35.0
124-125	29.80909459885577	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23478.0
3	6461.0
4	3367.0
5	2703.0
6	2956.0
7	3107.0
8	3324.0
9	3536.0
10	3820.0
11	4057.0
12	4702.0
13	5898.0
14	8040.0
15	7344.0
16	4209.0
17	3761.0
18	3310.0
19	2940.0
20	2730.0
21	2819.0
22	3098.0
23	3675.0
24	4833.0
25	6182.0
26	7543.0
27	8967.0
28	10821.0
29	12949.0
30	15792.0
31	19772.0
32	25558.0
33	35074.0
34	54417.0
35	97692.0
36	225292.0
37	461768.0
38	162782.0
39	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48713108678503	18.078842898125174	11.214230840425794	27.219795174664007
2	16.031816131102538	19.80009277322031	36.88609227758256	27.28199881809459
3	18.764530056459648	23.319280659908884	28.737611215018617	29.178578068612854
4	13.515519825849509	14.775182918484713	33.93897731142449	37.77031994424129
5	14.486815867038477	37.470028839049505	32.75663178968944	15.286523504222577
6	35.013273562560606	33.7293147026563	16.119191822849604	15.13821991193349
7	30.392553429051194	31.78787158749824	19.132976411192107	18.686598572258458
8	28.463404614085093	34.187028338806996	18.405960979013088	18.943606068094823
9	28.114802487567324	14.716438141745572	18.239141126619785	38.92961824406731
10-11	27.027980767816974	24.870420404966286	26.01912170805438	22.082477119162363
12-13	26.93621906350993	22.167937511697485	26.77099397711247	24.124849447680113
14-15	24.210176215759997	23.532190986635005	24.1309812467267	28.126651550878297
16-17	24.371048753381817	26.69788129144947	24.859770602705293	24.071299352463416
18-19	24.363957225157492	25.701137862934413	26.787671621997966	23.147233289910123
20-21	24.937356599444513	24.714152244516615	26.828257774614052	23.52023338142482
22-23	24.965795465421948	24.135911047955453	26.66365097514199	24.23464251148061
24-25	24.464957267672883	24.565637084804973	26.678920346974028	24.29048530054812
26-27	24.08506048231759	24.875671485182647	26.90051256470603	24.138755467793732
28-29	24.167439798943242	25.14698780251448	26.698640452187067	23.98693194635521
30-31	24.668511282352096	24.739640319798696	26.55106774971316	24.04078064813605
32-33	24.364481838820424	24.821152535479555	26.54945531553888	24.26491031016114
34-35	24.301934241661456	24.967413139435223	26.79275392134513	23.93789869755819
36-37	24.402899950592495	24.81349063243198	26.804128624739658	23.979480792235872
38-39	24.41948068298119	25.012500193609032	26.647712176638162	23.920306946771614
40-41	24.46147253439431	24.83360346252538	26.58944983003903	24.115474173041278
42-43	24.260949582732145	24.745276570766347	26.401797986994858	24.591975859506647
44-45	24.1815650833934	24.881835349906765	26.46367884156743	24.4729207251324
46-47	24.26004599877755	24.93990014667025	26.324271921006968	24.47578193354523
48-49	24.237914819933597	25.209574417385504	26.11530762998618	24.43720313269472
50-51	24.317438707789062	25.315421613921323	26.068763877697155	24.29837580059246
52-53	24.523867392958525	25.129190038427385	25.864020435298546	24.48292213331554
54-55	24.033248460715733	24.994211425355587	26.12213952073382	24.85040059319486
56-57	24.242216044479743	25.124305003971404	25.84396346306593	24.789515488482923
58-59	24.224027005559968	25.130619539316918	26.018149324861	24.62720413026211
60-61	24.511768973943404	25.052154094150996	26.12101020683323	24.315066725072366
62-63	24.2059077816093	25.114843083858986	26.08755913917429	24.59168999535743
64-65	24.057017770297477	24.870036878152774	26.17737255671317	24.895572794836582
66-67	24.170845370356396	25.15518724537576	25.94230330835775	24.731664075910096
68-69	24.018160423160733	25.165061360133407	25.98408341848679	24.832694798219066
70-71	23.981968379135836	24.976201548755945	26.14914649242401	24.892683579684206
72-73	24.123839245361548	25.275638271086198	25.801656411066993	24.79886607248526
74-75	23.94743499771434	25.317083109434037	25.812818994444914	24.92266289840671
76-77	23.906663277706734	24.98280367596248	25.869705081057038	25.24082796527375
78-79	23.81643968249619	25.02278578738746	26.02488386694768	25.135890663168674
80-81	24.007494771689842	25.2987463969735	25.711451289941074	24.98230754139559
82-83	23.975515637932652	25.24107850660007	25.840438596073163	24.94296725939412
84-85	23.88129349973689	25.28452427049514	25.58250181197192	25.251680417796045
86-87	23.662660612178925	25.36304289636866	25.815223218859884	25.159073272592536
88-89	23.669011519870757	25.118854391009997	25.792485058560434	25.419649030558812
90-91	23.723113582208104	24.968347895154885	25.921564733915808	25.386973788721207
92-93	23.78295344520421	25.010821986463977	25.901600542012737	25.304624026319072
94-95	23.757217215038654	24.96348202378295	25.876332141617464	25.40296861956093
96-97	23.497745416766282	24.901250286936126	25.965991814079686	25.6350124822179
98-99	23.45140176560552	24.946118014950734	25.8469509369131	25.75552928253064
100-101	23.42703916010265	25.055619053368076	26.00167909573467	25.5156626907946
102-103	23.283012693478042	24.913442727640263	26.045895410061853	25.75764916881984
104-105	23.333195659132446	25.061377541578274	25.865143354585463	25.74028344470382
106-107	23.390541321403255	24.872269912093106	25.968534613712045	25.76865415279159
108-109	23.252221149481343	24.951935163205984	25.927489512314168	25.868354174998505
110-111	23.063487606725765	25.31633001974965	25.86080565714556	25.75937671637903
112-113	23.037218309076064	25.237727600547732	25.955437790507418	25.769616299868787
114-115	22.900117632014336	25.421183770435686	25.790878713529906	25.887819884020075
116-117	22.767812286658245	25.276701402361507	25.933421685034	26.02206462594625
118-119	22.902574481402286	25.1216071525022	25.880440549908972	26.095377816186538
120-121	22.681030203141443	25.2262356281896	26.00401119958697	26.08872296908199
122-123	22.599728513184743	25.35355637402273	25.823257158697466	26.223457954095057
124-125	22.46569798483488	25.303085779984468	25.75423087672345	26.4769853584572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	59.0
2	45.5
3	66.5
4	77.0
5	94.0
6	221.5
7	527.5
8	865.5
9	970.5
10	747.0
11	544.0
12	529.5
13	563.5
14	628.5
15	675.5
16	718.5
17	753.0
18	786.5
19	819.5
20	888.5
21	980.0
22	1053.0
23	1156.5
24	1328.0
25	1579.5
26	1977.5
27	2578.0
28	3267.0
29	4181.0
30	5494.0
31	6879.5
32	8609.5
33	10326.0
34	12100.5
35	14161.0
36	16650.0
37	19417.0
38	21833.0
39	24508.5
40	26986.0
41	29230.5
42	31774.5
43	35298.5
44	39163.5
45	42564.5
46	46199.0
47	49774.0
48	53186.5
49	55799.0
50	57447.5
51	58408.0
52	57871.0
53	57232.0
54	56896.5
55	55032.5
56	51635.0
57	47315.0
58	43079.5
59	38437.5
60	32900.5
61	27968.5
62	23496.5
63	18185.0
64	13238.0
65	9943.5
66	7539.5
67	5864.0
68	4600.5
69	3415.5
70	2602.5
71	2002.0
72	1469.5
73	1136.0
74	885.5
75	664.5
76	459.0
77	274.0
78	144.0
79	73.5
80	44.0
81	28.0
82	16.5
83	11.0
84	7.5
85	3.5
86	2.0
87	1.5
88	0.5
89	1.0
90	1.5
91	1.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022239678604873192
2	0.002144540436898486
3	0.03280352594218795
4	0.17211922691700074
5	0.02390765450023868
6	0.06918128594587339
7	0.05242209956862966
8	0.044479357209746384
9	0.03431264699037578
10-11	0.0641376445479825
12-13	0.05778345066087588
14-15	0.057505454678314964
16-17	0.04976128087840376
18-19	0.037132320527779344
20-21	0.023748799653061013
22-23	0.0032168106553477293
24-25	0.005520205939423881
26-27	0.003812516332263975
28-29	0.005003927686096468
30-31	0.002501963843048234
32-33	0.00929300855989344
34-35	0.0054010648040406315
36-37	0.007386750393761452
38-39	0.002422536419459401
40-41	0.0036139477732918934
42-43	0.003772802620469559
44-45	0.005440778515835049
46-47	0.005123068821479717
48-49	0.0024622501312538177
50-51	0.0017076896071599056
52-53	0.0010325565066548268
54-55	0.00520249624506855
56-57	8.737016594771611E-4
58-59	8.737016594771611E-4
60-61	1.1914113538324922E-4
62-63	8.339879476827447E-4
64-65	0.0
66-67	0.003812516332263975
68-69	7.545605240939118E-4
70-71	3.971371179441641E-5
72-73	0.0019062581661319876
74-75	0.0059173430573680455
76-77	0.0016679758953654894
78-79	1.9856855897208204E-4
80-81	0.0
82-83	0.006751331005050789
84-85	0.0018665444543375714
86-87	0.0
88-89	9.928427948604102E-4
90-91	8.737016594771611E-4
92-93	0.0
94-95	0.0033359517907309787
96-97	0.0015885484717766563
98-99	7.545605240939118E-4
100-101	0.0
102-103	0.001429693624598991
104-105	0.0
106-107	6.751331005050789E-4
108-109	0.0027402461138147324
110-111	1.1914113538324922E-4
112-113	5.559919651218297E-4
114-115	0.0
116-117	0.0021842541486929027
118-119	0.0030976695199644803
120-121	0.002859387249197982
122-123	0.001429693624598991
124-125	0.10504276769623141
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1259011.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.65860266138354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3228186266416	48.90934155681088
2	11.153590958448122	13.754296663091056
3	3.755990249351785	6.94767331154438
4	1.8151696986487158	4.476833088478579
5	1.0367282907885422	3.1961608874773013
6	0.6459766630468962	2.389801103720101
7	0.447793696571536	1.9327233527823542
8	0.3269129216604338	1.6125595153226153
9	0.23645310871290676	1.3121431450360241
>10	1.2132529973605204	12.573484439975594
>50	0.03523946638247144	1.415324859652495
>100	0.009427604537284641	0.8712196066775553
>500	2.5828713967999126E-4	0.09109456995538245
>1k	3.8743070951998695E-4	0.5173438994756292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2744	0.21794885032775727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2200	0.17474033189543223	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1553	0.12335078883345736	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	7.942742358883282E-5
14-15	0.0	0.0	0.0	3.971371179441641E-5	2.3828227076649845E-4
16-17	0.0	0.0	0.0	1.5885484717766563E-4	3.1770969435533127E-4
18-19	0.0	0.0	0.0	2.7799598256091486E-4	3.1770969435533127E-4
20-21	0.0	0.0	0.0	3.574234061497477E-4	3.574234061497477E-4
22-23	0.0	0.0	0.0	5.162782533274133E-4	5.162782533274133E-4
24-25	0.0	0.0	0.0	0.001072270218449243	6.354193887106625E-4
26-27	0.0	0.0	0.0	0.0024622501312538173	6.354193887106625E-4
28-29	0.0	0.0	0.0	0.006393907598901042	6.354193887106625E-4
30-31	0.0	0.0	0.0	0.019539146202852874	6.354193887106625E-4
32-33	0.0	0.0	0.0	0.0488875792189266	7.942742358883283E-4
34-35	0.0	0.0	0.0	0.08657589171182778	8.737016594771611E-4
36-37	0.0	0.0	0.0	0.1461861731152468	9.134153712715775E-4
38-39	0.0	0.0	0.0	0.22879069364763294	0.0010325565066548266
40-41	0.0	0.0	0.0	0.32029108562196834	0.0010325565066548266
42-43	0.0	0.0	0.0	0.40988521943017175	0.0012311250656269087
44-45	0.0	0.0	0.0	0.5150471282617864	0.0013502662010101579
46-47	0.0	0.0	0.0	0.6329571385794088	0.0013899799128045743
48-49	0.0	0.0	0.0	0.753527967587257	0.0014296936245989907
50-51	0.0	0.0	0.0	0.866354622795194	0.0014296936245989907
52-53	0.0	0.0	0.0	0.9874417300563697	0.0014296936245989907
54-55	0.0	0.0	0.0	1.1240171849173677	0.0014296936245989907
56-57	0.0	0.0	0.0	1.2746910074653837	0.0014296936245989907
58-59	0.0	0.0	0.0	1.4325530118481886	0.0014296936245989907
60-61	0.0	0.0	0.0	1.5883896169294789	0.0014296936245989907
62-63	0.0	0.0	0.0	1.754075222535784	0.0015885484717766565
64-65	0.0	0.0	0.0	1.9233747759153812	0.0015885484717766565
66-67	0.0	0.0	0.0	2.1164231289480395	0.0015885484717766565
68-69	0.0	0.0	0.0	2.330082898402	0.0015885484717766565
70-71	0.0	0.0	0.0	2.5545845111758356	0.0015885484717766565
72-73	0.0	0.0	0.0	2.7682045669180013	0.0019062581661319878
74-75	0.0	0.0	0.0	2.9871462600406193	0.002025399301515237
76-77	0.0	0.0	0.0	3.2404006001536128	0.0021842541486929027
78-79	0.0	0.0	0.0	3.5300724139820856	0.0024622501312538173
80-81	0.0	0.0	0.0	3.8274884016104704	0.002501963843048234
82-83	0.0	0.0	0.0	4.128240341029586	0.0027799598256091486
84-85	0.0	0.0	0.0	4.4368953090957906	0.0027799598256091486
86-87	0.0	7.942742358883282E-5	0.0	4.7775198151565	0.002819673537403565
88-89	0.0	7.942742358883282E-5	0.0	5.1553957828803725	0.003018242096375647
90-91	0.0	7.942742358883282E-5	0.0	5.554200876719902	0.003018242096375647
92-93	0.0	7.942742358883282E-5	0.0	5.952529406017899	0.003018242096375647
94-95	0.0	7.942742358883282E-5	0.0	6.356139858984552	0.0030976695199644803
96-97	0.0	7.942742358883282E-5	0.0	6.815389222175183	0.0032168106553477297
98-99	0.0	7.942742358883282E-5	0.0	7.309507224321313	0.003256524367142146
100-101	0.0	7.942742358883282E-5	0.0	7.840280982453688	0.003256524367142146
102-103	0.0	1.5885484717766563E-4	0.0	8.382015725041322	0.0034153792143198115
104-105	0.0	1.5885484717766563E-4	0.0	8.93097836317554	0.003534520349703061
106-107	0.0	1.5885484717766563E-4	0.0	9.5166364710078	0.003574234061497477
108-109	0.0	1.5885484717766563E-4	0.0	10.160435452907084	0.0036139477732918934
110-111	0.0	1.5885484717766563E-4	0.0	10.840771049657231	0.003772802620469559
112-113	0.0	1.5885484717766563E-4	0.0	11.533735606758004	0.0038125163322639756
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1990	0.0	53.682167	1
GTATCAA	3400	0.0	51.07921	1
TCAACGC	4615	0.0	36.96428	4
CAACGCA	4640	0.0	36.461548	5
TATCAAC	4775	0.0	36.10762	2
ATCAACG	4890	0.0	34.892456	3
AACGCAG	4895	0.0	34.819366	6
GTACATG	6465	0.0	34.709583	1
TACATGG	6615	0.0	33.820168	2
CATGGGG	5610	0.0	32.109383	4
ATGGGGG	3520	0.0	31.30861	5
ACATGGG	6920	0.0	31.294996	3
GTATAGG	305	0.0	29.350904	1
ACGCAGA	5860	0.0	28.77922	7
CGCAGAG	6165	0.0	27.451002	8
TATACCG	160	8.661667E-6	26.062302	5
GCAGAGT	6760	0.0	24.946676	9
CTTACAC	625	0.0	23.863512	3
ATACCGT	200	1.8009905E-6	23.837873	6
GAGTACT	3195	0.0	23.481106	12-13
>>END_MODULE