FastQCFastQC Report
Mon 15 Aug 2016
SRR2921619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781136
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.034.6785471
GTATCAA45600.033.42281
GTACATG112000.032.584431
CGCCTTA4750.032.5762372
CATGGGG83750.030.840834
TACATGG118650.030.1458742
ACATGGG120550.029.4238993
TCAACGC54100.026.5119064
CAACGCA55250.026.0670595
TATCAAC58250.025.9512882
AACGCAG58300.024.9040056
ATCAACG59100.024.5710373
ATGGGGG42900.023.860535
ATGGGGA32800.021.7729055
ACGCAGA68300.021.163487
AGTACAT83850.020.8672582
GAGTACA84800.020.8510651
CGCAGAG72650.019.8138588
ATGGGGC30200.019.3120375
GCAGAGT78350.018.1446329
Produced by FastQC (version 0.11.5)