FastQCFastQC Report
Mon 15 Aug 2016
SRR2921619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781136
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.2447875962307202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.19650380431365153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23340.13103996550516075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.050.5141681
GTATCAA68450.044.8921131
TCAACGC85900.035.5501824
CAACGCA88000.034.6618235
TATCAAC89150.034.3938032
ATCAACG89600.034.1535263
AACGCAG90550.033.9660646
GTACATG113100.031.3899521
TACATGG119000.029.5762752
CATGGGG83600.029.108444
ACGCAGA105700.029.0404957
ACATGGG122850.028.2115633
CGCAGAG109900.027.874868
GAGTACT52050.025.5078312-13
GCAGAGT120300.025.2668939
ATGGGGG42100.025.1884825
GTATAGG5600.024.5062071
GTACTTT56200.023.4038214-15
AGTACTT55350.023.39543312-13
CAGAGTA117550.022.131410-11
Produced by FastQC (version 0.11.5)