FastQCFastQC Report
Mon 15 Aug 2016
SRR2921618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856322
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG126300.033.5616761
GTATCAA52000.033.430781
GGTATCA38550.032.585551
CATGGGG99400.032.5742234
TACATGG128950.032.263942
ATGGGGG61200.031.410695
ACATGGG130950.031.3166453
TCAACGC60750.027.9228734
CAACGCA61400.027.431185
ATCAACG63850.026.7536183
AACGCAG64200.026.6020246
TATCAAC65350.026.5038472
TGGGGGG48250.023.5561286
ATGGGGA32700.023.1143865
GTATAGG5000.022.623021
ACGCAGA76300.022.4534747
GTACCGT3859.094947E-1221.6388996
TTAACGC1954.030339E-521.3660853
TACCGTA2255.0885083E-621.150627
CGCAGAG80800.021.129358
Produced by FastQC (version 0.11.5)