##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921618_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1856322
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.928609368417764	31.0	31.0	34.0	26.0	34.0
2	30.24288135355827	31.0	31.0	34.0	26.0	34.0
3	30.319466665804747	31.0	31.0	34.0	26.0	34.0
4	33.73758485866137	37.0	35.0	37.0	29.0	37.0
5	33.80628791772117	37.0	35.0	37.0	30.0	37.0
6	33.772848137338244	37.0	35.0	37.0	29.0	37.0
7	33.696142695071224	37.0	35.0	37.0	28.0	37.0
8	33.649635677430965	37.0	35.0	37.0	29.0	37.0
9	35.328495271833226	39.0	37.0	39.0	29.0	39.0
10-11	35.35371314890412	39.0	37.0	39.0	29.0	39.0
12-13	35.28315750176962	39.0	37.0	39.0	28.5	39.0
14-15	36.57508907398609	40.0	38.0	41.0	28.0	41.0
16-17	36.54870491218657	40.0	38.0	41.0	28.0	41.0
18-19	36.52153694240547	40.0	38.0	41.0	27.0	41.0
20-21	36.58035163080543	40.0	38.0	41.0	28.0	41.0
22-23	36.52572263863705	40.0	38.0	41.0	28.0	41.0
24-25	36.52577004420569	40.0	38.0	41.0	28.0	41.0
26-27	36.48852946848661	40.0	38.0	41.0	29.0	41.0
28-29	36.432861863405165	40.0	38.0	41.0	28.5	41.0
30-31	36.336378333069376	40.0	37.0	41.0	28.5	41.0
32-33	36.2345172335403	40.0	37.0	41.0	28.0	41.0
34-35	36.10195402521761	40.0	37.0	41.0	27.0	41.0
36-37	35.996016585484625	40.0	37.0	41.0	27.0	41.0
38-39	35.873973642503834	40.0	36.0	41.0	26.0	41.0
40-41	35.759461451192195	40.0	36.0	41.0	26.0	41.0
42-43	35.529467409210255	40.0	35.5	41.0	25.0	41.0
44-45	35.231199921134376	40.0	35.0	41.0	24.0	41.0
46-47	34.985390465662746	39.5	35.0	41.0	23.5	41.0
48-49	34.7943295936804	39.0	35.0	41.0	23.0	41.0
50-51	34.58857972916337	39.0	35.0	41.0	23.0	41.0
52-53	34.38494075920018	39.0	34.0	40.5	22.0	41.0
54-55	34.143001052619105	38.0	34.0	40.0	20.5	41.0
56-57	33.88765095710765	38.0	34.0	40.0	20.0	41.0
58-59	33.881351672824	38.0	34.0	40.0	20.0	41.0
60-61	33.659901407191214	37.0	34.0	40.0	19.5	41.0
62-63	33.33862524928326	37.0	34.0	40.0	18.0	41.0
64-65	33.03589409595965	36.0	33.0	39.5	17.0	41.0
66-67	32.71037460095824	36.0	33.0	39.0	14.0	41.0
68-69	32.37461361768055	35.0	33.0	39.0	12.5	40.5
70-71	32.01166634883388	35.0	33.0	37.5	9.0	40.0
72-73	31.668527873935666	35.0	32.5	37.0	7.0	39.5
74-75	31.307537970244386	35.0	32.0	37.0	6.5	39.0
76-77	31.010711773065232	35.0	32.0	36.0	2.0	39.0
78-79	30.740470942002517	35.0	32.0	36.0	2.0	37.0
80-81	30.487455840096708	35.0	32.0	35.0	2.0	37.0
82-83	30.267370370011236	35.0	31.0	35.0	2.0	36.5
84-85	30.059855725461425	35.0	31.0	35.0	2.0	36.0
86-87	29.88803074035647	35.0	31.0	35.0	2.0	36.0
88-89	29.729182760318523	35.0	31.0	35.0	2.0	35.5
90-91	29.542816925080885	35.0	31.0	35.0	2.0	35.0
92-93	29.461489439870885	34.0	31.0	35.0	2.0	35.0
94-95	29.332776317901743	34.0	31.0	35.0	2.0	35.0
96-97	29.24695877116147	34.0	30.0	35.0	2.0	35.0
98-99	29.15041194361754	34.0	30.0	35.0	2.0	35.0
100-101	29.039480219487785	34.0	30.0	35.0	2.0	35.0
102-103	28.180762281543828	33.5	28.0	34.5	2.0	35.0
104-105	29.07983501784712	34.0	30.5	35.0	2.0	35.0
106-107	29.33507575733089	35.0	31.0	35.0	2.0	35.0
108-109	29.32567895009594	35.0	31.0	35.0	2.0	35.0
110-111	29.257450485422247	35.0	31.0	35.0	2.0	35.0
112-113	29.172996926179835	35.0	31.0	35.0	2.0	35.0
114-115	29.052211038817617	35.0	31.0	35.0	2.0	35.0
116-117	28.929156148556125	35.0	31.0	35.0	2.0	35.0
118-119	28.636004152296856	35.0	30.0	35.0	2.0	35.0
120-121	28.511585813237144	35.0	30.0	35.0	2.0	35.0
122-123	28.2586097670555	34.0	30.0	35.0	2.0	35.0
124-125	27.502919213369232	34.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74879.0
3	13349.0
4	6628.0
5	5647.0
6	6119.0
7	6913.0
8	6810.0
9	7185.0
10	8034.0
11	8146.0
12	8037.0
13	8137.0
14	8491.0
15	7466.0
16	6530.0
17	5882.0
18	5408.0
19	5117.0
20	4929.0
21	5082.0
22	5996.0
23	8179.0
24	12514.0
25	15946.0
26	17024.0
27	17768.0
28	21300.0
29	25399.0
30	31033.0
31	38081.0
32	50455.0
33	68469.0
34	107029.0
35	177251.0
36	355295.0
37	550144.0
38	145535.0
39	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2845306784896	17.381244382561313	12.510480497115035	24.82374444183405
2	16.647675386492754	19.43861237046196	38.35899176505766	25.554720477987626
3	19.91388543487209	23.825891693047694	28.098702031967555	28.161520840112665
4	13.862571944703797	15.325306164010488	34.4881480815015	36.32397380978421
5	14.866843142315417	37.596102090067234	31.969930532244884	15.567124235372464
6	33.980119194241745	33.28233893355018	17.569179466494685	15.168362405713387
7	29.731339550563373	32.751660632169504	19.208513303654065	18.308486513613058
8	27.677022356151564	34.47463124665499	18.743293127581353	19.105053269612092
9	28.0659100130779	16.31110629738178	18.061076525902802	37.56190716363752
10-11	26.75587798952479	25.61980130246196	25.85846472263058	21.76585598538267
12-13	26.435286055096878	23.142128878343343	26.20022024960185	24.222364816957924
14-15	24.337469635147574	24.318636421441518	23.610814737218742	27.73307920619217
16-17	24.661889710017192	27.16185670261734	24.30015555242397	23.8760980349415
18-19	24.731270301252124	26.463212662538016	25.551727873353062	23.2537891628568
20-21	25.57364999637608	25.310491605309295	25.315826443174682	23.800031955139943
22-23	25.66052815306485	24.763396017254987	25.152519738442898	24.423556091237266
24-25	25.093172387778196	25.069030554507734	25.12324190332489	24.714555154389178
26-27	24.579928571755797	25.35613740976815	25.385780158060523	24.678153860415534
28-29	24.760305082672144	25.798554136867892	25.24746938919599	24.193671391263972
30-31	25.498329024324796	25.20829269501811	25.19681517748122	24.09656310317588
32-33	25.056325027064574	25.25813055335668	25.144537482749534	24.541006936829213
34-35	24.986782246333654	25.691513528458458	25.203898334589987	24.117805890617902
36-37	25.086012387076757	25.565047238121508	25.19704998793187	24.15189038686987
38-39	25.063729016302023	25.646894641697713	25.121517534572078	24.167858807428182
40-41	24.965763594453406	25.409449086247715	25.287054946938287	24.33773237236059
42-43	24.883619617803028	25.353963668360297	25.048797723769724	24.71361899006695
44-45	24.64588372750228	25.36126877599518	25.432120317231714	24.560727179270835
46-47	24.560232732069306	25.3929428170538	25.33237816482638	24.714446286050517
48-49	24.58098330938516	25.696196699597763	25.29961710764446	24.42320288337262
50-51	24.60305913226692	25.616474157418413	25.403353352139696	24.377113358174974
52-53	24.677144500618997	25.53638615762014	25.165526336705152	24.62094300505571
54-55	24.461192612796882	25.26631886706849	25.438611143740523	24.8338773763941
56-57	24.566100455567916	25.459104097363127	25.058309945142714	24.916485501926243
58-59	24.51647925343621	25.657634552282634	25.2676228602833	24.558263333997857
60-61	24.92238829855591	25.495784060713188	25.4349771145501	24.1468505261808
62-63	24.71054485594934	25.4798388346088	25.309001364598306	24.50061494484356
64-65	24.53702369120742	25.271815634413393	25.264784003659678	24.926376670719506
66-67	24.64323810072938	25.40314465086842	25.179192614109247	24.774424634292952
68-69	24.404997484984673	25.445165795683767	25.349791888206692	24.800044831124872
70-71	24.30045916988316	25.384072068032275	25.498089778909122	24.81737898317545
72-73	24.36136106471254	25.714801444043324	25.12912872460801	24.794708766636134
74-75	24.405927605247264	25.57995699652341	25.245039541971494	24.769075856257835
76-77	24.344753325633977	25.15461345760061	25.31727513935214	25.183358077413274
78-79	24.208724599681116	25.38092058929871	25.471122671059305	24.93923213996087
80-81	24.43263082944024	25.58176875373965	25.20460474154432	24.780995675275793
82-83	24.481102985690274	25.541056844747285	25.329858169002588	24.647982000559857
84-85	24.486768364759747	25.62683114215859	25.04215869772995	24.844241795351714
86-87	24.16999355321721	25.726246380251343	25.238656235073464	24.865103831457983
88-89	24.170406583618867	25.491990587748447	25.31800295170105	25.01959987693163
90-91	24.449446496453213	25.30242657894666	25.349546447689942	24.898580476910187
92-93	24.419259915330848	25.317374631363787	25.427763465121117	24.835601988184248
94-95	24.420364715849473	25.29493926116688	25.356304406680124	24.928391616303518
96-97	24.158087182483655	25.248238643144372	25.38721833982435	25.20645583454762
98-99	24.010064745806176	25.369333395108885	25.240763028625423	25.379838830459512
100-101	23.97090915360108	25.563643230368356	25.397947812004258	25.067499804026312
102-103	23.85292583072119	25.381855246120704	25.5516345330477	25.213584390110405
104-105	23.96726805602432	25.44725746466156	25.398044799956036	25.187429679358075
106-107	23.929250869536293	25.37696516009892	25.534509271237948	25.159274699126843
108-109	23.92931292679924	25.4132791893707	25.470713311866476	25.18669457196358
110-111	23.670779698269943	25.87732553284903	25.47351040648173	24.978384362399293
112-113	23.533276247574776	25.92349772110384	25.503623796411247	25.03960223491014
114-115	23.608508533640506	25.98272367469691	25.40362250049298	25.0051452911696
116-117	23.543241335840236	25.805350687691586	25.43491846288944	25.216489513578743
118-119	23.5102120194314	25.598803972173716	25.574656526900498	25.316327481494387
120-121	23.491941642054567	25.72175063062189	25.623032883918434	25.163274843405105
122-123	23.29365600967081	25.825240207982723	25.58258112339116	25.298522658955303
124-125	23.14799303089191	25.81012639074129	25.535193563518533	25.50668701484826
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	532.0
1	449.0
2	346.5
3	287.5
4	245.5
5	237.0
6	228.0
7	243.0
8	295.5
9	347.0
10	370.0
11	376.5
12	400.5
13	438.5
14	502.0
15	533.5
16	568.0
17	676.5
18	722.5
19	791.0
20	881.0
21	977.0
22	1179.0
23	1399.0
24	1644.0
25	2059.0
26	2800.0
27	3856.5
28	4970.5
29	6359.5
30	8282.0
31	10311.0
32	12788.0
33	15386.0
34	17875.5
35	20705.5
36	24105.5
37	28090.5
38	31856.0
39	35603.0
40	39630.0
41	43362.0
42	46923.0
43	51487.0
44	56758.5
45	61689.5
46	66655.5
47	71526.0
48	77219.0
49	82617.5
50	86870.5
51	88543.0
52	85717.5
53	83499.5
54	82671.5
55	80067.5
56	75866.0
57	69977.0
58	63859.0
59	57121.0
60	48886.5
61	41767.0
62	35132.0
63	27568.5
64	20899.0
65	16053.5
66	12344.5
67	9550.0
68	7399.0
69	5669.5
70	4518.0
71	3597.0
72	2775.0
73	2255.0
74	1775.0
75	1349.5
76	996.5
77	644.5
78	398.5
79	275.0
80	207.0
81	146.5
82	111.5
83	76.5
84	56.5
85	57.0
86	53.0
87	37.0
88	31.5
89	31.0
90	26.5
91	21.0
92	17.0
93	14.5
94	16.0
95	12.5
96	10.5
97	11.0
98	7.5
99	6.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026611762398980346
2	0.011151082624673951
3	0.010235293230377057
4	0.011258822553414764
5	0.019016097422753165
6	0.10073683337265842
7	0.062219808847818435
8	0.05112259618751488
9	0.027958511508240486
10-11	0.05330432974451631
12-13	0.013252011235119768
14-15	0.030059440118686304
16-17	0.03649690086094977
18-19	0.04487368032054784
20-21	0.032079563782576516
22-23	0.03999844854502613
24-25	0.03350711783839226
26-27	0.04813281316495737
28-29	0.047971203271846154
30-31	0.028012381472610898
32-33	0.030975229512983198
34-35	0.04648977925166
36-37	0.01045077308785868
38-39	0.02246377514245912
40-41	0.04530464003551108
42-43	0.024052939091386085
44-45	0.01769628329567823
46-47	0.024456963824164128
48-49	0.031163774388279622
50-51	0.030544269798019958
52-53	0.02690804720301758
54-55	0.02499566346786818
56-57	0.02728513695361042
58-59	0.01896222745838276
60-61	0.024160679020126894
62-63	0.025022598450053387
64-65	0.022760059946496353
66-67	0.03057120478020516
68-69	0.02523807830753501
70-71	0.048940862630513456
72-73	0.02273312496431115
74-75	0.010396903123488274
76-77	0.017373063509455795
78-79	0.02634241257712832
80-81	0.019689471977383233
82-83	0.026207737666202307
84-85	0.012928791448897336
86-87	0.019258512262419987
88-89	0.023864394216089668
90-91	0.02246377514245912
92-93	0.008403714441783268
94-95	0.011824457179304021
96-97	0.015918574471454845
98-99	0.007541795011856779
100-101	0.011070277678118344
102-103	0.004767491846780892
104-105	0.004982971704262515
106-107	8.888544121116918E-4
108-109	0.015218264934639573
110-111	0.0014544890380009503
112-113	0.002720433200705481
114-115	0.013790710878823826
116-117	0.006033436009485423
118-119	0.2798275299220717
120-121	0.0010504643052229084
122-123	7.541795011856779E-4
124-125	0.31621669085428067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1856322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.6890104250401
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34689202321994	50.995561620882455
2	10.02061567476475	12.563649610012954
3	3.3083589026812237	6.221932372198724
4	1.5863158789175875	3.9777829068348516
5	0.9441736521815325	2.959465596232814
6	0.615850907107358	2.316425036155414
7	0.4127719566032806	1.811338584346708
8	0.30005534313132465	1.5048138026918874
9	0.2225096353314719	1.255401796406986
>10	1.182998471286962	12.800868067019003
>50	0.04710195167532242	1.9818779238897828
>100	0.011755237006793916	1.2568321036343737
>500	4.288329232160152E-4	0.20624723214985224
>1k	1.7153316928640588E-4	0.14780334754430224
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	5.386996437040556E-5
5	0.0	0.0	0.0	0.0	5.386996437040556E-5
6	0.0	0.0	0.0	0.0	5.386996437040556E-5
7	0.0	0.0	0.0	0.0	5.386996437040556E-5
8	0.0	0.0	0.0	0.0	5.386996437040556E-5
9	0.0	0.0	0.0	0.0	5.386996437040556E-5
10-11	0.0	0.0	0.0	0.0	1.0773992874081112E-4
12-13	0.0	0.0	0.0	0.0	1.3467491092601392E-4
14-15	0.0	0.0	0.0	0.0	2.6934982185202784E-4
16-17	0.0	0.0	0.0	0.0	3.7708975059283896E-4
18-19	0.0	0.0	0.0	2.693498218520278E-5	4.309597149632445E-4
20-21	0.0	0.0	0.0	5.386996437040556E-5	5.117646615188529E-4
22-23	0.0	0.0	0.0	1.8854487529641945E-4	5.925696080744613E-4
24-25	0.0	0.0	0.0	5.925696080744613E-4	6.464395724448667E-4
26-27	0.0	0.0	0.0	0.001239009180519328	6.464395724448667E-4
28-29	0.0	0.0	0.0	0.003932507399039607	6.464395724448667E-4
30-31	0.0	0.0	0.0	0.013548296039157	7.003095368152723E-4
32-33	0.0	0.0	0.0	0.034288232321763146	8.080494655560835E-4
34-35	0.0	0.0	0.0	0.05976872546896497	8.080494655560835E-4
36-37	0.0	2.693498218520278E-5	0.0	0.0958885365793219	8.080494655560835E-4
38-39	0.0	5.386996437040556E-5	0.0	0.15296376382976662	9.157893942968946E-4
40-41	0.0	5.386996437040556E-5	0.0	0.21876592530821698	9.696593586673002E-4
42-43	0.0	5.386996437040556E-5	0.0	0.2829519878555552	9.965943408525029E-4
44-45	0.0	5.386996437040556E-5	0.0	0.3582621980453822	0.0010773992874081114
46-47	0.0	5.386996437040556E-5	0.0	0.43677767111524834	0.0011582042339637198
48-49	0.0	8.080494655560834E-5	0.0	0.5154816890604108	0.001239009180519328
50-51	0.0	1.0773992874081112E-4	0.0	0.5946166667205366	0.001239009180519328
52-53	0.0	1.0773992874081112E-4	0.0	0.6824516436264829	0.0014544890380009503
54-55	0.0	1.0773992874081112E-4	0.0	0.7830807370704005	0.0016969038776677753
56-57	0.0	1.0773992874081112E-4	0.0	0.8911977555618045	0.0017777088242233836
58-59	0.0	1.0773992874081112E-4	0.0	1.0041361358643597	0.0018315787885937892
60-61	0.0	1.0773992874081112E-4	0.0	1.119471729581398	0.0018315787885937892
62-63	0.0	1.0773992874081112E-4	0.0	1.248167074462297	0.0020470586460754115
64-65	0.0	1.6160989311121669E-4	0.0	1.3752732553942688	0.0020470586460754115
66-67	0.0	1.6160989311121669E-4	0.0	1.5254627160589596	0.0020470586460754115
68-69	0.0	1.6160989311121669E-4	0.0	1.6854834452212493	0.0020470586460754115
70-71	0.0	1.6160989311121669E-4	0.0	1.8531537093241366	0.0020470586460754115
72-73	0.0	1.6160989311121669E-4	0.0	2.007679702120645	0.002235603521371831
74-75	0.0	1.6160989311121669E-4	0.0	2.177854919566756	0.002262538503557034
76-77	0.0	1.6160989311121669E-4	0.0	2.386223941751485	0.002262538503557034
78-79	0.0	1.6160989311121669E-4	0.0	2.6137706712520776	0.002262538503557034
80-81	0.0	1.6160989311121669E-4	0.0	2.8318632220056648	0.002343343450112642
82-83	0.0	1.6160989311121669E-4	0.0	3.057524502753294	0.002478018361038656
84-85	0.0	1.6160989311121669E-4	0.0	3.2991043579723778	0.002478018361038656
86-87	0.0	1.6160989311121669E-4	0.0	3.5749455105310393	0.002478018361038656
88-89	0.0	1.6160989311121669E-4	0.0	3.8940442444791366	0.002478018361038656
90-91	0.0	2.1547985748162225E-4	0.0	4.216429046253829	0.002478018361038656
92-93	0.0	2.1547985748162225E-4	0.0	4.519797750605767	0.002478018361038656
94-95	0.0	2.1547985748162225E-4	0.0	4.834371407546751	0.0026665632363350755
96-97	0.0	2.1547985748162225E-4	0.0	5.1871658042085365	0.0027473681828906835
98-99	0.0	2.1547985748162225E-4	0.0	5.602126139753771	0.002828173129446292
100-101	0.0	2.1547985748162225E-4	0.0	6.043725172680171	0.002855108111631495
102-103	0.0	2.1547985748162225E-4	0.0	6.482388292548383	0.0030975229512983198
104-105	0.0	2.1547985748162225E-4	0.0	6.933064414471197	0.00328606782659474
106-107	0.0	2.1547985748162225E-4	0.0	7.4208838768274035	0.003366872773150348
108-109	0.0	2.1547985748162225E-4	0.0	7.962896523340239	0.0034207427375207533
110-111	0.0	2.1547985748162225E-4	0.0	8.538335482744912	0.003555417648446767
112-113	0.0	2.1547985748162225E-4	0.0	9.103215929132984	0.003555417648446767
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	12630	0.0	33.561676	1
GTATCAA	5200	0.0	33.43078	1
GGTATCA	3855	0.0	32.58555	1
CATGGGG	9940	0.0	32.574223	4
TACATGG	12895	0.0	32.26394	2
ATGGGGG	6120	0.0	31.41069	5
ACATGGG	13095	0.0	31.316645	3
TCAACGC	6075	0.0	27.922873	4
CAACGCA	6140	0.0	27.43118	5
ATCAACG	6385	0.0	26.753618	3
AACGCAG	6420	0.0	26.602024	6
TATCAAC	6535	0.0	26.503847	2
TGGGGGG	4825	0.0	23.556128	6
ATGGGGA	3270	0.0	23.114386	5
GTATAGG	500	0.0	22.62302	1
ACGCAGA	7630	0.0	22.453474	7
GTACCGT	385	9.094947E-12	21.638899	6
TTAACGC	195	4.030339E-5	21.366085	3
TACCGTA	225	5.0885083E-6	21.15062	7
CGCAGAG	8080	0.0	21.12935	8
>>END_MODULE