FastQCFastQC Report
Mon 15 Aug 2016
SRR2921618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856322
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.21343279883554683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.1810030802845627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.11732878239874332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.045.017661
GTATCAA72450.043.0820271
TCAACGC91450.033.269274
TATCAAC92300.033.033772
CAACGCA93000.032.87175
ATCAACG95250.031.9463083
AACGCAG96700.031.9341346
GTACATG125450.031.7788831
CATGGGG98450.030.964354
TACATGG128650.030.610692
ACATGGG131800.029.56063
ATGGGGG60850.028.4004465
ACGCAGA113800.027.0780947
TATCACG2256.362825E-926.5189552
CGCAGAG118200.026.0168768
GAGTACT52000.023.9876512-13
GCAGAGT127400.023.810659
AGTACTT54400.023.53133612-13
TGGGGGG44950.023.2092046
GTACTTT53500.023.19536814-15
Produced by FastQC (version 0.11.5)