FastQCFastQC Report
Mon 15 Aug 2016
SRR2921617_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921617_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527063
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23200.040.544471
GTATCAA33850.037.285481
GTACATG98350.036.8037261
TACATGG101800.035.3132632
ACATGGG102350.034.8908923
CATGGGG84800.034.461514
TCAACGC39000.031.8955674
CAACGCA39550.031.7509085
ATGGGGG53750.031.667065
AACGCAG42000.030.1792056
ATCAACG42300.029.5479663
TATCAAC43550.028.5631942
TGGGGGG42300.025.0413156
ACGCAGA51600.024.3274387
CGCAGAG55800.022.6007398
GAGTACA69750.022.3624631
ATGCCTA7200.022.3192672
ATGGGGA27750.022.30445
AGTACAT69100.022.05012
ATGGGGC20850.021.1225265
Produced by FastQC (version 0.11.5)