##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921617_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1527063
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.842170231352604	31.0	31.0	34.0	26.0	34.0
2	29.78945465904157	31.0	31.0	34.0	26.0	34.0
3	30.014529197551116	31.0	31.0	34.0	26.0	34.0
4	33.50805304037882	37.0	35.0	37.0	28.0	37.0
5	33.54645355168713	37.0	35.0	37.0	28.0	37.0
6	33.394016487859375	37.0	35.0	37.0	28.0	37.0
7	33.444188615662874	37.0	35.0	37.0	28.0	37.0
8	33.470126641795396	37.0	35.0	37.0	28.0	37.0
9	35.11682753101869	39.0	37.0	39.0	28.0	39.0
10-11	35.148347514149705	39.0	37.0	39.0	28.0	39.0
12-13	35.0704620569027	39.0	37.0	39.0	27.0	39.0
14-15	36.32823138272619	40.0	38.0	41.0	27.0	41.0
16-17	36.28514769855599	40.0	38.0	41.0	27.0	41.0
18-19	36.22042869220196	40.0	37.5	41.0	27.0	41.0
20-21	36.28355378920189	40.0	38.0	41.0	27.0	41.0
22-23	36.21276004984732	40.0	37.0	41.0	27.0	41.0
24-25	36.19253888019027	40.0	37.0	41.0	27.0	41.0
26-27	36.16063417160916	40.0	37.0	41.0	27.0	41.0
28-29	36.11562260365159	40.0	37.0	41.0	27.0	41.0
30-31	36.0190329410116	40.0	37.0	41.0	27.0	41.0
32-33	35.926215224912134	40.0	37.0	41.0	27.0	41.0
34-35	35.77686807944401	40.0	36.0	41.0	25.5	41.0
36-37	35.665337972303696	40.0	36.0	41.0	25.0	41.0
38-39	35.53586787185597	40.0	36.0	41.0	25.0	41.0
40-41	35.41904328767052	40.0	35.5	41.0	24.5	41.0
42-43	35.131893052218544	40.0	35.0	41.0	23.5	41.0
44-45	34.69595622446487	39.0	35.0	41.0	22.5	41.0
46-47	34.40849133270861	39.0	34.5	41.0	21.0	41.0
48-49	34.19775477501583	39.0	34.0	40.0	20.0	41.0
50-51	33.973376016575614	38.0	34.0	40.0	18.5	41.0
52-53	33.7538120562151	38.0	33.5	40.0	18.0	41.0
54-55	33.504223139451355	38.0	33.0	40.0	15.5	41.0
56-57	33.24209217301447	37.5	33.0	40.0	13.5	41.0
58-59	33.25195915296226	37.0	33.0	40.0	13.5	41.0
60-61	33.032258001143376	37.0	33.0	40.0	12.0	41.0
62-63	32.71030762974415	36.0	33.0	39.5	8.0	41.0
64-65	32.40771173160505	36.0	32.5	39.0	7.5	41.0
66-67	32.085221762297955	35.0	32.0	39.0	6.5	40.5
68-69	31.75801980664845	35.0	32.0	38.5	2.0	40.0
70-71	31.399989063974438	35.0	32.0	37.0	2.0	39.5
72-73	31.063597572595235	35.0	31.0	37.0	2.0	39.0
74-75	30.70756347315075	35.0	31.0	36.0	2.0	39.0
76-77	30.421521574420964	35.0	31.0	36.0	2.0	38.0
78-79	30.167975060622908	35.0	31.0	35.5	2.0	37.0
80-81	29.92325005582612	35.0	31.0	35.0	2.0	37.0
82-83	29.714794347057065	34.5	30.5	35.0	2.0	36.0
84-85	29.515860838747322	34.0	30.0	35.0	2.0	36.0
86-87	29.355710602640492	34.0	30.0	35.0	2.0	36.0
88-89	29.203999114640325	34.0	30.0	35.0	2.0	35.0
90-91	29.021176271051033	34.0	30.0	35.0	2.0	35.0
92-93	28.946892171442826	34.0	30.0	35.0	2.0	35.0
94-95	28.821307961754034	34.0	29.0	35.0	2.0	35.0
96-97	28.739392873771415	34.0	29.0	35.0	2.0	35.0
98-99	28.645942898230132	34.0	29.0	35.0	2.0	35.0
100-101	28.54390486836496	34.0	29.0	35.0	2.0	35.0
102-103	27.61906810655487	32.5	27.0	34.0	2.0	35.0
104-105	28.593804905233117	34.0	29.5	35.0	2.0	35.0
106-107	28.89864006920474	34.0	30.0	35.0	2.0	35.0
108-109	28.91222366071341	34.0	30.0	35.0	2.0	35.0
110-111	28.85960500647321	34.0	30.0	35.0	2.0	35.0
112-113	28.77808643127363	34.0	30.0	35.0	2.0	35.0
114-115	28.65635144064128	34.0	30.0	35.0	2.0	35.0
116-117	28.535025404976743	34.0	30.0	35.0	2.0	35.0
118-119	28.243376664878923	34.0	29.0	35.0	2.0	35.0
120-121	28.105671475243653	34.0	29.0	35.0	2.0	35.0
122-123	27.840683717698617	34.0	29.0	35.0	2.0	35.0
124-125	27.006492528468048	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67846.0
3	11871.0
4	6031.0
5	5162.0
6	5464.0
7	5993.0
8	6195.0
9	6492.0
10	6791.0
11	7208.0
12	7003.0
13	6755.0
14	6417.0
15	6077.0
16	5634.0
17	5208.0
18	4811.0
19	4400.0
20	4503.0
21	4599.0
22	5265.0
23	7134.0
24	10049.0
25	12526.0
26	13893.0
27	15632.0
28	18777.0
29	23154.0
30	28491.0
31	36070.0
32	47216.0
33	66145.0
34	104589.0
35	166904.0
36	305378.0
37	396774.0
38	84566.0
39	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25084810377816	17.16166407164487	12.281079834748313	25.30640798982865
2	16.651199466627194	19.736234574005803	38.28161481497976	25.330951144387242
3	20.127787576076898	23.64954296555983	28.104687312734423	28.11798214562885
4	14.546685816866717	14.944884231015335	33.634798349834334	36.873631602283616
5	15.283579665208718	37.55935609201455	31.314407500121167	15.842656742655564
6	34.503906754382115	32.70524634411555	16.906372260175882	15.884474641326454
7	30.623620348826925	31.46682514295299	19.484523991269825	18.425030516950258
8	27.91431139623314	35.20287428162614	18.313914636351488	18.568899685789237
9	28.644360223597182	16.406908089087782	17.689041668358797	37.25969001895624
10-11	27.154227212898007	25.33910148180616	25.50816903217099	21.998502273124846
12-13	26.768177230659607	22.787645829471945	25.906904914566997	24.53727202530145
14-15	24.669142692448776	23.968846617408165	23.318398574647592	28.04361211549547
16-17	25.281500585306354	26.906012311413114	23.78164895899318	24.03083814428735
18-19	25.221998568543665	26.33437444211692	24.969839509832664	23.473787479506754
20-21	26.04874760786667	25.06461175467217	24.806918034859397	24.079722602601763
22-23	26.158355696487373	24.452729156410406	24.605889329655657	24.783025817446568
24-25	25.486629201485943	24.947611414586788	24.55349334751761	25.01226603640966
26-27	24.898255337827656	25.27693154346587	24.918368416570033	24.90644470213644
28-29	25.13884202767996	25.69231021210384	24.651281631316028	24.517566128900175
30-31	25.89485991638706	25.026700138044873	24.735872507454914	24.342567438113157
32-33	25.264893081484978	25.076566882166425	24.75379215272567	24.904747883622925
34-35	25.214416717412146	25.63026214804228	24.78517382470027	24.3701473098453
36-37	25.38051534815914	25.506750727531657	24.69380143354927	24.418932490759932
38-39	25.385551768620797	25.61610830393246	24.66670007116468	24.33163985628206
40-41	25.30932682660304	25.251249738358293	24.83052660163212	24.608896833406543
42-43	25.17643313749847	25.206563257039722	24.617355669358616	24.999647936103187
44-45	24.80607385813059	25.224987727644415	25.07532884837001	24.893609565854984
46-47	24.731112717326194	25.16207252399525	25.064771696758093	25.042043061920467
48-49	24.836264153402894	25.49291064806217	24.896921009510876	24.77390418902406
50-51	24.86059821991501	25.39262583006493	25.066675400601003	24.680100549419056
52-53	24.98865183840218	25.275811090609096	24.906381760671366	24.829155310317365
54-55	24.684725401769192	24.981872488430813	25.24299380684293	25.090408302957062
56-57	24.89224028525006	25.175767631375805	24.774335878867635	25.1576562045065
58-59	24.815888959625255	25.371693402392985	25.02341504693489	24.78900259104687
60-61	25.203567020161348	25.353333658651927	25.092412860739294	24.350686460447427
62-63	25.102975810141327	25.30946781393908	24.99702789066699	24.590528485252605
64-65	24.834638031107733	24.928826479398897	25.0863203068788	25.150215182614566
66-67	24.872069990095742	25.199624529023673	24.87531245578456	25.052993025096033
68-69	24.641148873805644	25.234449839115094	25.136003013009983	24.988398274069283
70-71	24.681246521580043	25.11076075316985	25.232814599028874	24.975178126221234
72-73	24.76910506002237	25.59469713716802	24.83876408450128	24.797433718308337
74-75	24.70063801890611	25.39632225521542	24.954155898760508	24.948883827117964
76-77	24.687575422850998	24.94484409799919	25.060804940501708	25.3067755386481
78-79	24.601306761425974	25.17005911539784	25.141500319321402	25.087133803854783
80-81	24.744437556307247	25.42063212140325	24.915418369188217	24.919511953101285
82-83	24.853654654459923	25.36105723663569	25.037057123974176	24.74823098493021
84-85	24.73830399111383	25.482081936409656	24.800097192486316	24.9795168799902
86-87	24.407768332025032	25.711845484819936	24.843879817014603	25.036506366140433
88-89	24.404236149933535	25.353593807363712	24.97850779761665	25.263662245086106
90-91	24.807088834437323	25.053136128703073	25.067906171257825	25.071868865601783
92-93	24.841455112485253	25.19399174882207	25.11356912399918	24.850984014693502
94-95	24.75869934896716	25.153982152511507	25.028726852738735	25.058591645782595
96-97	24.432314045824874	25.16916535485673	25.051077900060548	25.347442699257844
98-99	24.26004501781655	25.211614518082115	24.96386590008455	25.564474564016788
100-101	24.26535647132714	25.489866048593978	25.06472276780948	25.180054712269396
102-103	24.202338580021546	25.303455479552976	25.258956316163445	25.23524962426203
104-105	24.248742870371494	25.398422583506573	25.068197870255247	25.28463667586668
106-107	24.283201035722808	25.234447213400745	25.200722046010952	25.281629704865495
108-109	24.271165548943127	25.179578586733303	25.218318767833008	25.33093709649056
110-111	24.076332138644972	25.741484980390126	25.142711014860808	25.039471866104098
112-113	23.97133247414579	25.816293653537866	25.18064549083708	25.03172838147927
114-115	24.014588094597972	25.902251121008685	25.096218615768358	24.98694216862499
116-117	23.96265288427031	25.699554412261655	25.116144893292088	25.221647810175956
118-119	23.921981163111848	25.425727724686986	25.286516252611037	25.36577485959013
120-121	23.861263984534826	25.59196067696805	25.391606266748045	25.15516907174908
122-123	23.825324588364687	25.659351579631178	25.26905643857648	25.24626739342765
124-125	23.691905578161492	25.62094435633074	25.206286249740117	25.480863815767652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	579.0
1	449.0
2	284.0
3	207.5
4	163.0
5	152.0
6	140.5
7	146.0
8	161.0
9	181.5
10	185.5
11	193.5
12	226.5
13	251.0
14	279.0
15	318.5
16	369.0
17	394.5
18	425.0
19	483.5
20	545.5
21	616.5
22	709.5
23	864.5
24	1048.0
25	1304.5
26	1666.5
27	2201.5
28	2927.0
29	3974.0
30	5407.5
31	7143.5
32	9486.0
33	11823.5
34	14018.5
35	16545.5
36	19636.5
37	22511.5
38	25088.5
39	28022.5
40	30820.5
41	33552.5
42	36489.0
43	40613.0
44	44938.5
45	49132.5
46	53514.0
47	57707.5
48	62382.0
49	66913.5
50	70258.5
51	71702.5
52	71062.0
53	70524.0
54	70377.5
55	68211.5
56	64741.5
57	59886.5
58	54869.0
59	49132.5
60	41744.5
61	35780.5
62	30485.5
63	23995.0
64	18211.0
65	14483.5
66	11378.5
67	8955.0
68	7269.5
69	5817.5
70	4761.5
71	3817.0
72	2973.5
73	2452.0
74	1983.0
75	1560.0
76	1164.5
77	773.0
78	506.5
79	335.5
80	227.5
81	153.0
82	114.0
83	77.0
84	62.0
85	56.0
86	43.0
87	34.0
88	38.0
89	30.5
90	20.5
91	19.5
92	17.0
93	13.5
94	7.0
95	8.0
96	8.0
97	5.5
98	4.0
99	3.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02763474722391938
2	0.011525392207132253
3	0.010084718181240722
4	0.01218024403708295
5	0.01709163276171317
6	0.08984567106923552
7	0.05644822774175001
8	0.04577414291355366
9	0.025473736185082085
10-11	0.04911388724630222
12-13	0.012704125501043507
14-15	0.027700232406914452
16-17	0.033528413693475645
18-19	0.04227068562331744
20-21	0.029108163841308446
22-23	0.03644250433675625
24-25	0.03208773966758412
26-27	0.04538123181558325
28-29	0.045053805900607896
30-31	0.025244538044599342
32-33	0.029893986037249284
34-35	0.042860052270273064
36-37	0.010051975589743186
38-39	0.021118971515909954
40-41	0.042139715257327306
42-43	0.023018041822766972
44-45	0.016862434621230427
46-47	0.02321449737175218
48-49	0.028977193475318307
50-51	0.029501074939278865
52-53	0.025146310270106734
54-55	0.024753399172136314
56-57	0.02635778615551552
58-59	0.017484543859683586
60-61	0.02321449737175218
62-63	0.024196775116678226
64-65	0.022035764077840927
66-67	0.029271876798796122
68-69	0.0229525566397719
70-71	0.04502106330911036
72-73	0.022297704809821205
74-75	0.009757292266265373
76-77	0.016665979072245218
78-79	0.025408251002087014
80-81	0.01856504937910224
82-83	0.023967576976195483
84-85	0.012835095867033646
86-87	0.01797568273214661
88-89	0.022690615907791624
90-91	0.021217199290402555
92-93	0.008480331197861516
94-95	0.011885560713605137
96-97	0.014963364314373407
98-99	0.007334340495447797
100-101	0.010870540377181557
102-103	0.0044529924436647345
104-105	0.004747675767142547
106-107	8.840499704334399E-4
108-109	0.014701423582393128
110-111	0.00170261475787181
112-113	0.0025539221368077155
114-115	0.01319526437350653
116-117	0.005959151652551335
118-119	0.2750377685792924
120-121	0.0012442184769063229
122-123	8.840499704334399E-4
124-125	0.3093847470602064
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1527063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.18828833872095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.57322310086143	53.82807076556488
2	9.65683151921227	12.59024635025716
3	3.074192897572287	6.01204119047371
4	1.4517366297509338	3.785449040483474
5	0.8273208095098668	2.6965813739476605
6	0.5231146300597733	2.046056840312391
7	0.3677413370941832	1.6780699821593674
8	0.26465257469484516	1.3801798679033959
9	0.19357866824340758	1.1357155837509172
>10	1.014546452624181	11.440584914066106
>50	0.04005193882066118	1.798939791231053
>100	0.012509122178659201	1.351058384769483
>500	4.002555020094107E-4	0.176381724461247
>1k	1.0006387550235267E-4	0.0806241906191203
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	6.548518299506962E-5
9	0.0	0.0	0.0	0.0	6.548518299506962E-5
10-11	0.0	0.0	0.0	0.0	6.548518299506962E-5
12-13	0.0	0.0	0.0	0.0	3.274259149753481E-4
14-15	0.0	0.0	0.0	0.0	3.929110979704177E-4
16-17	0.0	0.0	0.0	0.0	5.566240554580918E-4
18-19	0.0	0.0	0.0	0.0	5.893666469556266E-4
20-21	0.0	0.0	0.0	0.0	6.221092384531613E-4
22-23	0.0	0.0	0.0	6.548518299506962E-5	7.530796044433006E-4
24-25	0.0	0.0	0.0	5.893666469556266E-4	8.51307378935905E-4
26-27	0.0	0.0	0.0	0.001047762927921114	8.51307378935905E-4
28-29	0.0	0.0	0.0	0.0028813480517830632	8.51307378935905E-4
30-31	0.0	0.0	0.0	0.012245729220078019	8.51307378935905E-4
32-33	0.0	0.0	0.0	0.0317930563441063	8.840499704334398E-4
34-35	0.0	0.0	0.0	0.05530223703933629	0.001047762927921114
36-37	0.0	0.0	0.0	0.08621124341300915	0.0011787332939112534
38-39	6.548518299506962E-5	0.0	0.0	0.13499770474433603	0.0013097036599013924
40-41	6.548518299506962E-5	0.0	0.0	0.19301757687796772	0.001375188842896462
42-43	6.548518299506962E-5	0.0	0.0	0.2454057232740234	0.001375188842896462
44-45	6.548518299506962E-5	0.0	0.0	0.31206963956300426	0.0014406740258915318
46-47	6.548518299506962E-5	0.0	0.0	0.37771853551556156	0.0015716443918816709
48-49	6.548518299506962E-5	0.0	0.0	0.44716557208183294	0.0016371295748767406
50-51	6.548518299506962E-5	0.0	0.0	0.5210001159087738	0.00170261475787181
52-53	6.548518299506962E-5	0.0	0.0	0.6023654557801479	0.0017680999408668796
54-55	6.548518299506962E-5	0.0	0.0	0.696467663744063	0.0018335851238619493
56-57	6.548518299506962E-5	0.0	0.0	0.793385734576766	0.0018335851238619493
58-59	6.548518299506962E-5	0.0	0.0	0.8970487792579612	0.0018335851238619493
60-61	6.548518299506962E-5	0.0	0.0	0.9996313184197377	0.0018335851238619493
62-63	6.548518299506962E-5	0.0	0.0	1.1171117367128927	0.0018335851238619493
64-65	6.548518299506962E-5	0.0	0.0	1.2418610103185004	0.001899070306857019
66-67	6.548518299506962E-5	0.0	0.0	1.380460400127565	0.001899070306857019
68-69	6.548518299506962E-5	0.0	0.0	1.5282604581474373	0.001899070306857019
70-71	6.548518299506962E-5	0.0	0.0	1.680186082695999	0.001931812898354554
72-73	6.548518299506962E-5	0.0	0.0	1.82467913897462	0.002062783264344693
74-75	6.548518299506962E-5	0.0	0.0	1.9921574944845104	0.002095525855842228
76-77	6.548518299506962E-5	0.0	0.0	2.195587215458694	0.002095525855842228
78-79	6.548518299506962E-5	0.0	0.0	2.4118847748914094	0.002095525855842228
80-81	6.548518299506962E-5	0.0	0.0	2.6211754197436514	0.0021282684473397625
82-83	6.548518299506962E-5	0.0	0.0	2.83357661078816	0.0022264962218323673
84-85	6.548518299506962E-5	0.0	0.0	3.068439219599977	0.0022264962218323673
86-87	6.548518299506962E-5	0.0	0.0	3.3236349777317633	0.002259238813329902
88-89	6.548518299506962E-5	0.0	0.0	3.6131122291614686	0.0022919814048274366
90-91	6.548518299506962E-5	0.0	0.0	3.914475041304779	0.0022919814048274366
92-93	6.548518299506962E-5	0.0	0.0	4.203264698313037	0.0022919814048274366
94-95	6.548518299506962E-5	0.0	0.0	4.5093751862234885	0.0022919814048274366
96-97	6.548518299506962E-5	0.0	0.0	4.85841121158721	0.0025539221368077155
98-99	6.548518299506962E-5	0.0	0.0	5.261014116640898	0.0026194073198027848
100-101	6.548518299506962E-5	0.0	0.0	5.694067631787293	0.0026194073198027848
102-103	6.548518299506962E-5	0.0	0.0	6.129511356113009	0.0027176350942953896
104-105	6.548518299506962E-5	0.0	0.0	6.5752035115774525	0.002750377685792924
106-107	6.548518299506962E-5	0.0	0.0	7.065229135929559	0.002750377685792924
108-109	6.548518299506962E-5	0.0	0.0	7.584493894488963	0.002783120277290459
110-111	6.548518299506962E-5	0.0	0.0	8.14118343513005	0.0028813480517830632
112-113	6.548518299506962E-5	0.0	0.0	8.67498590431436	0.0028813480517830632
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2320	0.0	40.54447	1
GTATCAA	3385	0.0	37.28548	1
GTACATG	9835	0.0	36.803726	1
TACATGG	10180	0.0	35.313263	2
ACATGGG	10235	0.0	34.890892	3
CATGGGG	8480	0.0	34.46151	4
TCAACGC	3900	0.0	31.895567	4
CAACGCA	3955	0.0	31.750908	5
ATGGGGG	5375	0.0	31.66706	5
AACGCAG	4200	0.0	30.179205	6
ATCAACG	4230	0.0	29.547966	3
TATCAAC	4355	0.0	28.563194	2
TGGGGGG	4230	0.0	25.041315	6
ACGCAGA	5160	0.0	24.327438	7
CGCAGAG	5580	0.0	22.600739	8
GAGTACA	6975	0.0	22.362463	1
ATGCCTA	720	0.0	22.319267	2
ATGGGGA	2775	0.0	22.3044	5
AGTACAT	6910	0.0	22.0501	2
ATGGGGC	2085	0.0	21.122526	5
>>END_MODULE