FastQCFastQC Report
Mon 15 Aug 2016
SRR2921617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527063
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29880.195669726789268No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.16436780931762476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.10150203364235791No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.058.7761961
GTATCAA50050.048.865781
TCAACGC63700.038.012474
ATCAACG65150.037.2592163
TATCAAC65350.037.1451872
CAACGCA65900.036.6928675
GTACATG100400.036.4804081
AACGCAG68000.035.669516
TACATGG103050.035.073442
ACATGGG106600.033.7375763
CATGGGG90200.033.0600054
ATGGGGG54850.031.9235925
ACGCAGA81550.029.7428177
CGCAGAG85200.028.6756448
GCAGAGT92750.026.148669
TGGGGGG41500.025.5613376
CGCGATA600.001290369324.78501104-105
GAGTACT38100.023.82872812-13
GTAAGTA9600.023.6083762
CAGAGTA90350.023.34769610-11
Produced by FastQC (version 0.11.5)