FastQCFastQC Report
Mon 15 Aug 2016
SRR2921616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786278
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.047.299691
GTATCAA37850.041.2086641
GTACATG99650.035.546171
TACATGG104700.033.936832
ACATGGG105600.033.0276223
CATGGGG89500.032.5184174
TCAACGC48600.031.7180924
AGCCTCG12150.031.35072
ATGGGGG58550.031.2045945
CAACGCA50400.030.8188935
TATCAAC51150.029.7877662
AACGCAG53250.029.2779126
ATCAACG54250.028.1953163
ACGCAGA65050.023.5937487
GTATAGG4950.022.8508071
CGCAGAG67500.022.8242348
TGGGGGG47900.022.3612126
GTACACT16050.021.1370014
GTACTAG3201.21053745E-820.464251
AGAGTAC66650.020.43700810-11
Produced by FastQC (version 0.11.5)