FastQCFastQC Report
Mon 15 Aug 2016
SRR2921616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1786278
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.20780639967575035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.1790314833413388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.1123565312902023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.057.8745731
GTATCAA55850.047.6520651
TCAACGC71900.036.2526444
CAACGCA73200.035.5618935
ATCAACG74750.035.033963
TATCAAC75550.034.900842
GTACATG98750.034.7456781
AACGCAG75800.034.2817656
GTATACG1251.2500386E-633.416281
TACATGG103250.032.702012
ACATGGG104800.031.5343883
CATGGGG86800.031.4039024
ATGGGGG53200.030.1200185
ACGCAGA87250.029.6446197
CGCAGAG92800.027.933568
GCAGAGT103100.024.9695139
GAGTACT46100.024.27986512-13
AGAGTAC93000.023.70848510-11
GTACTTT47300.023.52911414-15
CAGAGTA97400.023.0657410-11
Produced by FastQC (version 0.11.5)