FastQCFastQC Report
Mon 15 Aug 2016
SRR2921615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024098
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG3150.056.674883
GGTATCA24450.046.0107771
GCGTAGC6900.041.397312
GTATCAA38550.041.225271
GTACATG108650.037.526451
TACATGG111300.036.304012
TCAACGC43600.035.7597734
ACATGGG113250.034.9432563
CAACGCA45250.034.7171335
AACGCAG48000.033.0967676
CATGGGG102800.031.72254
TATCAAC49650.031.6420522
ATCAACG50050.031.032473
ATGGGGG61300.030.7721085
GTAGCGT4750.030.067524
GGCGTAG5550.028.9565891
AGCGTAG5850.026.4541681
ACGCAGA59300.026.3840487
CGCAGAG63100.024.8881978
GTATACG1200.001285443224.8007831
Produced by FastQC (version 0.11.5)