##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921615_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2024098
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.93599469986137	31.0	31.0	34.0	26.0	34.0
2	30.11424298625857	31.0	31.0	34.0	26.0	34.0
3	30.464319415364276	31.0	31.0	34.0	27.0	34.0
4	33.92903802088634	37.0	35.0	37.0	30.0	37.0
5	34.15860101635395	37.0	35.0	37.0	31.0	37.0
6	34.063375883974	37.0	35.0	37.0	30.0	37.0
7	33.9205922835752	37.0	35.0	37.0	30.0	37.0
8	34.016198820412846	37.0	35.0	37.0	30.0	37.0
9	35.68245855684853	39.0	37.0	39.0	30.0	39.0
10-11	35.73682795991103	39.0	37.0	39.0	30.0	39.0
12-13	35.68928974782841	39.0	37.0	39.0	30.0	39.0
14-15	37.0121543028055	40.5	38.0	41.0	31.0	41.0
16-17	36.98599968973834	41.0	38.0	41.0	30.5	41.0
18-19	36.93104731095036	40.5	38.0	41.0	30.0	41.0
20-21	36.98728001312189	41.0	38.0	41.0	30.0	41.0
22-23	36.915015478499555	40.0	38.0	41.0	30.0	41.0
24-25	36.89574887184316	40.5	38.0	41.0	30.0	41.0
26-27	36.85938576096612	40.0	38.0	41.0	30.0	41.0
28-29	36.81392699365347	40.0	38.0	41.0	30.0	41.0
30-31	36.72279553657975	40.0	38.0	41.0	30.0	41.0
32-33	36.63852837164999	40.0	38.0	41.0	30.0	41.0
34-35	36.52836251011561	40.0	38.0	41.0	29.5	41.0
36-37	36.43043987988724	40.0	37.0	41.0	29.0	41.0
38-39	36.32353102468359	40.0	37.0	41.0	29.0	41.0
40-41	36.21551550369597	40.0	37.0	41.0	28.0	41.0
42-43	35.99245392268556	40.0	36.5	41.0	27.5	41.0
44-45	35.667608238336285	40.0	36.0	41.0	26.5	41.0
46-47	35.43131829585326	40.0	35.0	41.0	26.0	41.0
48-49	35.2490706971698	39.0	35.0	41.0	26.0	41.0
50-51	35.05291492803214	39.0	35.0	41.0	25.0	41.0
52-53	34.84522093297854	39.0	35.0	41.0	24.0	41.0
54-55	34.61179498225876	38.5	34.5	40.0	24.0	41.0
56-57	34.36711018932878	38.0	34.0	40.0	23.0	41.0
58-59	34.36319387697631	38.0	34.0	40.0	23.5	41.0
60-61	34.14938728263157	38.0	34.0	40.0	23.0	41.0
62-63	33.838280063514716	37.0	34.0	40.0	22.5	41.0
64-65	33.53816341896489	36.5	34.0	40.0	22.0	41.0
66-67	33.21086800145052	36.0	34.0	39.0	21.5	41.0
68-69	32.874309692514885	35.5	33.0	39.0	20.5	41.0
70-71	32.5088958143331	35.0	33.0	38.5	20.0	40.0
72-73	32.16013280977502	35.0	33.0	37.0	19.5	39.5
74-75	31.792814873588135	35.0	33.0	37.0	18.0	39.0
76-77	31.492880285440727	35.0	32.0	36.0	18.0	39.0
78-79	31.219378458948135	35.0	32.0	36.0	16.0	37.5
80-81	30.953405418117107	35.0	32.0	35.5	13.0	37.0
82-83	30.725796132400703	35.0	32.0	35.0	11.0	37.0
84-85	30.508695725206984	35.0	32.0	35.0	7.5	36.0
86-87	30.330915548555453	35.0	32.0	35.0	7.0	36.0
88-89	30.16322529837982	35.0	31.0	35.0	7.0	36.0
90-91	29.976031298879796	35.0	31.0	35.0	3.5	35.0
92-93	29.896195243510938	35.0	31.0	35.0	2.0	35.0
94-95	29.76615287402092	34.0	31.0	35.0	2.0	35.0
96-97	29.67381026017515	34.0	31.0	35.0	2.0	35.0
98-99	29.576394522399607	34.0	31.0	35.0	2.0	35.0
100-101	29.465173623016277	34.0	31.0	35.0	2.0	35.0
102-103	28.5878507364762	33.5	29.0	34.5	2.0	35.0
104-105	29.503804410656006	34.0	31.0	35.0	2.0	35.0
106-107	29.769360722652756	35.0	31.5	35.0	2.0	35.0
108-109	29.763096203839936	35.0	32.0	35.0	2.0	35.0
110-111	29.693768779970142	35.0	32.0	35.0	2.0	35.0
112-113	29.601079097948812	35.0	31.5	35.0	2.0	35.0
114-115	29.477990937197703	35.0	31.0	35.0	2.0	35.0
116-117	29.35803330668772	35.0	31.0	35.0	2.0	35.0
118-119	29.056857918934753	35.0	31.0	35.0	2.0	35.0
120-121	28.924584432176704	35.0	31.0	35.0	2.0	35.0
122-123	28.667070467931886	34.5	30.5	35.0	2.0	35.0
124-125	27.89813536696346	34.0	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72493.0
3	12830.0
4	6327.0
5	5448.0
6	5909.0
7	6343.0
8	6683.0
9	7159.0
10	7570.0
11	7790.0
12	7432.0
13	7345.0
14	7391.0
15	6771.0
16	6266.0
17	5778.0
18	5392.0
19	5367.0
20	5156.0
21	5434.0
22	6183.0
23	8255.0
24	11910.0
25	15181.0
26	17052.0
27	18864.0
28	23217.0
29	27528.0
30	33350.0
31	41813.0
32	55013.0
33	75694.0
34	118350.0
35	195528.0
36	398081.0
37	615302.0
38	161808.0
39	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42275689143793	17.343266795084794	12.007671484158415	25.22630482931887
2	16.934121120848104	19.183940281516442	38.62629305063106	25.25564554700439
3	19.805921182655442	23.37607217676588	28.29271907980553	28.525287560773155
4	14.241485456225389	14.862467821867789	34.52455420008004	36.37149252182678
5	14.815872617432287	37.20338656423038	33.195603040278456	14.785137778058882
6	34.461386003483256	34.132098666532166	16.691441100637608	14.715074229346975
7	31.00956297997187	31.32351741678428	19.444561163277562	18.222358439966285
8	27.594192267692684	34.71748251195198	18.836179795719037	18.852145424636298
9	28.12626477016825	15.82841179705958	18.177357160965	37.86796627180717
10-11	26.81332641308915	25.139517065816463	26.46911841032105	21.57803811077334
12-13	26.49295186933516	22.61526325398643	26.706655114338645	24.18512976233976
14-15	24.203166627214372	23.937617275854553	23.759093588911714	28.10012250801936
16-17	24.78426366461914	26.96334768033709	24.45625448807192	23.796134166971843
18-19	24.768251427955732	26.425379443417913	25.67307851513857	23.133290613487787
20-21	25.435913984977564	25.384715743508675	25.375894424877206	23.80347584663656
22-23	25.628203414223933	24.866389779815893	25.167625935370385	24.337780870589786
24-25	24.964893427491184	25.28837056330883	25.179498799470124	24.567237209729864
26-27	24.601953840336694	25.50604365866859	25.403087690509317	24.488914810485397
28-29	24.745099638319143	25.79368992322777	25.276438104629197	24.184772333823894
30-31	25.2595699469383	25.404930599739323	25.193548586359622	24.14195086696276
32-33	24.817846688030624	25.46002560413224	25.238577782126676	24.483549925710456
34-35	24.850352750096445	25.720322196627134	25.34618244743595	24.083142605840475
36-37	24.921816152897687	25.707990279238434	25.1980134546131	24.17218011325078
38-39	25.00346523632912	25.768806844391495	25.17678882170352	24.050939097575867
40-41	24.850463215582074	25.549018212300222	25.29694827327149	24.30357029884621
42-43	24.781022357696962	25.42599060961409	25.186993601462298	24.605993431226654
44-45	24.631390764701568	25.47925562416447	25.418651495128326	24.47070211600564
46-47	24.592274646298705	25.440846437018923	25.406724679419785	24.56015423726259
48-49	24.497441215664097	25.6587346946743	25.3679047817429	24.4759193079187
50-51	24.56088634922078	25.63301922154604	25.400110153683908	24.405984275549265
52-53	24.651972530402215	25.604671630381016	25.369894234043066	24.373461605173702
54-55	24.459729707960268	25.275552302927906	25.55022748352863	24.714490505583193
56-57	24.534866367663366	25.50698948217346	25.216963528581566	24.741180621581606
58-59	24.493980889555964	25.584202691768425	25.43290035268467	24.488916065990946
60-61	24.823288377949392	25.43792460699454	25.44271795915866	24.296069055897405
62-63	24.605460852213948	25.507144609626575	25.486167750141636	24.40122678801784
64-65	24.47659915955424	25.194076359596934	25.52275844893086	24.806566031917963
66-67	24.49359860403263	25.524612029494804	25.316832901499993	24.664956464972576
68-69	24.423071268836768	25.395142534461552	25.40608800304991	24.775698193651778
70-71	24.345969099610212	25.287868497874104	25.632925157156233	24.733237245359454
72-73	24.454695385223573	25.564020680325893	25.268739228996928	24.712544705453602
74-75	24.352744672877247	25.50771118203538	25.381643416720546	24.757900728366828
76-77	24.315345254451394	25.204969622371653	25.484990800610046	24.994694322566904
78-79	24.27505937397152	25.22521587615029	25.585931902032698	24.91379284784549
80-81	24.395061764998005	25.52471464664808	25.2880464135584	24.792177174795512
82-83	24.420931273763415	25.522659189540985	25.381548868004156	24.674860668691448
84-85	24.394720978723825	25.54712282470131	25.239295208166574	24.81886098840829
86-87	24.186505019759085	25.58368674034992	25.40705941067398	24.822748829217012
88-89	24.220851916833876	25.31240170850842	25.477797025305684	24.988949349352023
90-91	24.426108239284513	25.14442726454687	25.509084179686248	24.920380316482373
92-93	24.371998273663813	25.34314884412306	25.510199292024293	24.774653590188837
94-95	24.353933590512295	25.241929805707596	25.46701698344642	24.93711962033369
96-97	24.169279755863514	25.214474257653723	25.53481445172563	25.081431534757133
98-99	24.14211114794775	25.224424899614668	25.414770416872738	25.218693535564846
100-101	24.0780318895711	25.243707601769955	25.612279913364876	25.065980595294064
102-103	23.88967830166679	25.198467594989744	25.687250248022735	25.224603855320733
104-105	24.00001482209777	25.261523563429932	25.66436347736295	25.074098137109345
106-107	23.897917965612	25.144139702126107	25.766571248736465	25.19137108352544
108-109	23.94206652422863	25.190729932345228	25.697696021946793	25.16950752147935
110-111	23.73486865686611	25.509610964253433	25.629172135231588	25.126348243648867
112-113	23.729933990880113	25.518956284675415	25.715988109909965	25.035121614534507
114-115	23.73674661111501	25.58001642436934	25.601090412101456	25.08214655241419
116-117	23.582843352445078	25.444503102436865	25.697594360795733	25.275059184322323
118-119	23.690516886887334	25.361865410244594	25.681123447431915	25.266494255436157
120-121	23.553082760704232	25.47423619878853	25.810538713943014	25.16214232656422
122-123	23.52003932646026	25.557406874472754	25.670223422217227	25.25233037684976
124-125	23.396241750905663	25.45472702749116	25.77142088241613	25.37761033918705
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	455.0
1	370.0
2	246.0
3	191.5
4	167.0
5	144.0
6	136.0
7	158.5
8	187.0
9	210.0
10	230.5
11	234.5
12	230.0
13	248.0
14	289.0
15	331.0
16	371.5
17	410.5
18	443.0
19	496.5
20	588.5
21	670.0
22	797.5
23	1004.5
24	1231.5
25	1544.0
26	2143.5
27	3026.5
28	4179.0
29	5655.5
30	7627.5
31	10022.5
32	13264.0
33	16489.0
34	19715.5
35	23467.0
36	27879.5
37	32462.0
38	36498.0
39	40867.5
40	45109.0
41	49090.0
42	53430.0
43	59486.5
44	65797.0
45	72182.0
46	77353.0
47	81508.0
48	87113.5
49	91273.5
50	93158.5
51	93300.5
52	92400.0
53	90976.0
54	90021.5
55	87798.5
56	82406.5
57	74864.5
58	68068.5
59	61684.0
60	52849.5
61	44350.5
62	36880.5
63	28597.5
64	21088.0
65	15860.5
66	12104.5
67	9476.0
68	7474.0
69	5756.5
70	4412.0
71	3377.5
72	2611.0
73	2079.0
74	1601.0
75	1170.0
76	848.0
77	571.5
78	390.0
79	269.5
80	175.0
81	121.0
82	95.5
83	72.0
84	60.0
85	54.5
86	43.5
87	37.5
88	33.5
89	29.5
90	26.0
91	21.0
92	14.5
93	10.5
94	10.0
95	12.5
96	12.5
97	8.0
98	8.0
99	6.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0247023612493071
2	0.009979753944720067
3	0.008794040604753327
4	0.010276182279711754
5	0.01659998675953437
6	0.09149754606743349
7	0.05893983394084674
8	0.04950353194361143
9	0.025147003751794628
10-11	0.05177614917854768
12-13	0.013018144378384841
14-15	0.02751843043172811
16-17	0.03354580657655904
18-19	0.041425859815088006
20-21	0.029198190996680994
22-23	0.03796752924018501
24-25	0.030458011420395656
26-27	0.04461246441624862
28-29	0.04473597622249515
30-31	0.02522111083554255
32-33	0.02946991697042337
34-35	0.04562526122747021
36-37	0.009016361855997091
38-39	0.021071114145658956
40-41	0.04199401412382207
42-43	0.02136754248065064
44-45	0.01524135689082248
46-47	0.022849684155609066
48-49	0.02858063196544831
50-51	0.027740751682971868
52-53	0.023664862076836202
54-55	0.021491054286897175
56-57	0.0240106951343265
58-59	0.016575284398285064
60-61	0.02282498179435976
62-63	0.02171337553814094
64-65	0.02025593622443182
66-67	0.02741962098673088
68-69	0.021219328313154796
70-71	0.047033295818680716
72-73	0.02151575664814648
74-75	0.009213980745991548
76-77	0.015933023005803078
78-79	0.02391188568932927
80-81	0.01872438982697478
82-83	0.024899980139301555
84-85	0.011758323954670179
86-87	0.017069331623271206
88-89	0.02280027943311045
90-91	0.02050295983692489
92-93	0.00783064851603035
94-95	0.010646717698451361
96-97	0.01541427341956763
98-99	0.0072624942072962865
100-101	0.010251479918462446
102-103	0.0044464250248752776
104-105	0.00459463919237112
106-107	9.88094449972284E-4
108-109	0.014524988414592573
110-111	0.0014574393137091188
112-113	0.0029395809886675448
114-115	0.01363570340961752
116-117	0.006150887951077467
118-119	0.2800259671221453
120-121	0.0013586298687118903
122-123	7.6577319872852E-4
124-125	0.31502921301241343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2024098.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.74544647427764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03359469078758	49.22421888907546
2	10.131580715162164	12.308947880654138
3	3.339102470040877	6.085058110979891
4	1.6027218327789448	3.8943221322491826
5	0.9289217853874069	2.821388429652058
6	0.6159880338541492	2.2451080883569645
7	0.41126273216796244	1.74876367986419
8	0.3293628626656446	1.6005835315736616
9	0.2431978899836358	1.3295847967792394
>10	1.2779788395634115	13.736559228596013
>50	0.06576532508302001	2.6888333937441273
>100	0.019790379225132437	1.9983120373689975
>500	6.510607111473734E-4	0.25657396610354505
>1k	8.138258889342158E-5	0.0617458350025679
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.9404722498614196E-5	0.0
10-11	0.0	0.0	0.0	4.9404722498614196E-5	0.0
12-13	0.0	0.0	0.0	4.9404722498614196E-5	1.235118062465355E-4
14-15	0.0	0.0	0.0	4.9404722498614196E-5	2.223212512437639E-4
16-17	0.0	0.0	0.0	4.9404722498614196E-5	2.9642833499168516E-4
18-19	0.0	0.0	0.0	1.235118062465355E-4	3.2113069624099225E-4
20-21	0.0	0.0	0.0	2.47023612493071E-4	3.9523777998891357E-4
22-23	0.0	0.0	0.0	3.705354187396065E-4	4.446425024875278E-4
24-25	0.0	0.0	0.0	5.187495862354491E-4	5.434519474847562E-4
26-27	0.0	0.0	0.0	0.0012104157012160477	5.434519474847562E-4
28-29	0.0	0.0	0.0	0.004026484883637057	6.422613924819846E-4
30-31	0.0	0.0	0.0	0.013759215215864054	6.422613924819846E-4
32-33	0.0	0.0	0.0	0.035225567141511926	6.422613924819846E-4
34-35	0.0	0.0	0.0	0.061533581872023985	7.41070837479213E-4
36-37	0.0	0.0	0.0	0.1012549787609098	7.41070837479213E-4
38-39	0.0	0.0	0.0	0.16582695106659856	7.41070837479213E-4
40-41	4.9404722498614196E-5	0.0	0.0	0.2366980254908606	7.41070837479213E-4
42-43	4.9404722498614196E-5	0.0	0.0	0.3077173140826185	7.41070837479213E-4
44-45	4.9404722498614196E-5	0.0	0.0	0.38555445437918523	7.41070837479213E-4
46-47	4.9404722498614196E-5	0.0	0.0	0.4733466462592226	8.398802824764414E-4
48-49	4.9404722498614196E-5	0.0	0.0	0.5652147277453957	9.88094449972284E-4
50-51	4.9404722498614196E-5	0.0	0.0	0.6584908438227793	9.88094449972284E-4
52-53	4.9404722498614196E-5	0.0	0.0	0.7586589186887196	0.0011610109787174336
54-55	4.9404722498614196E-5	0.0	0.0	0.8674975223531667	0.001259820423714662
56-57	4.9404722498614196E-5	0.0	0.0	0.9843643934236386	0.0012845227849639692
58-59	4.9404722498614196E-5	0.0	0.0	1.1133848262287696	0.0012845227849639692
60-61	4.9404722498614196E-5	0.0	0.0	1.2411207362489365	0.0012845227849639692
62-63	4.9404722498614196E-5	0.0	0.0	1.379750387580048	0.0014574393137091188
64-65	4.9404722498614196E-5	0.0	0.0	1.5203068230886054	0.001506844036207733
66-67	4.9404722498614196E-5	0.0	0.0	1.67593169895924	0.00153154639745704
68-69	4.9404722498614196E-5	0.0	0.0	1.8473907883906806	0.0015809511199556543
70-71	4.9404722498614196E-5	0.0	0.0	2.034511174854182	0.0016797605649528826
72-73	7.410708374792129E-5	0.0	0.0	2.2085640122168	0.0017044629262021897
74-75	9.880944499722839E-5	0.0	0.0	2.390916842959185	0.0017785700099501112
76-77	9.880944499722839E-5	0.0	0.0	2.6056297669381623	0.0017785700099501112
78-79	9.880944499722839E-5	0.0	0.0	2.8433405892402446	0.0017785700099501112
80-81	9.880944499722839E-5	0.0	0.0	3.0786799848623927	0.0018279747324487254
82-83	9.880944499722839E-5	0.0	0.0	3.3177000323106887	0.0018773794549473396
84-85	9.880944499722839E-5	0.0	0.0	3.575320957779712	0.0019267841774459537
86-87	1.235118062465355E-4	0.0	0.0	3.8644867985641014	0.0019267841774459537
88-89	1.4821416749584258E-4	0.0	0.0	4.185963327862583	0.002025593622443182
90-91	1.4821416749584258E-4	0.0	0.0	4.518259491388262	0.002025593622443182
92-93	1.4821416749584258E-4	0.0	0.0	4.849789881715214	0.0020749983449417963
94-95	1.4821416749584258E-4	0.0	0.0	5.187619374160738	0.002272617234936253
96-97	1.4821416749584258E-4	0.0	0.0	5.568060439761316	0.0023467243186841742
98-99	1.4821416749584258E-4	0.0	0.0	5.993484505196882	0.0024208314024320955
100-101	1.4821416749584258E-4	0.0	0.0	6.439040995050634	0.0024702361249307097
102-103	1.4821416749584258E-4	0.0	0.0	6.89519479788034	0.002519640847429324
104-105	1.4821416749584258E-4	0.0	0.0	7.37360542819567	0.0025690455699279384
106-107	1.4821416749584258E-4	0.0	0.0	7.887636863432502	0.0026184502924265526
108-109	1.4821416749584258E-4	0.0	0.0	8.430940596749762	0.002717259737423781
110-111	1.4821416749584258E-4	0.0	0.0	9.018436854342033	0.002717259737423781
112-113	1.4821416749584258E-4	0.0	0.0	9.600795020794447	0.002717259737423781
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	315	0.0	56.67488	3
GGTATCA	2445	0.0	46.010777	1
GCGTAGC	690	0.0	41.39731	2
GTATCAA	3855	0.0	41.22527	1
GTACATG	10865	0.0	37.52645	1
TACATGG	11130	0.0	36.30401	2
TCAACGC	4360	0.0	35.759773	4
ACATGGG	11325	0.0	34.943256	3
CAACGCA	4525	0.0	34.717133	5
AACGCAG	4800	0.0	33.096767	6
CATGGGG	10280	0.0	31.7225	4
TATCAAC	4965	0.0	31.642052	2
ATCAACG	5005	0.0	31.03247	3
ATGGGGG	6130	0.0	30.772108	5
GTAGCGT	475	0.0	30.06752	4
GGCGTAG	555	0.0	28.956589	1
AGCGTAG	585	0.0	26.454168	1
ACGCAGA	5930	0.0	26.384048	7
CGCAGAG	6310	0.0	24.888197	8
GTATACG	120	0.0012854432	24.800783	1
>>END_MODULE