FastQCFastQC Report
Mon 15 Aug 2016
SRR2921615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024098
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24180.11946061900164912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.049.7713171
GTATCAA47850.048.362811
TCAACGC61350.037.0290954
CAACGCA62250.036.366435
GTACATG108300.035.962221
ATCAACG65250.035.0934753
TATCAAC65600.034.9971432
AACGCAG65100.034.7889826
TACATGG112850.034.029852
ACATGGG114750.033.310493
CATGGGG101400.032.2236944
ATGGGGG58100.030.2483925
ACGCAGA78300.029.0749687
CGCAGAG82400.027.769498
GTATTAT5600.024.496361
GCAGAGT95350.023.7479889
TATACCG2802.57387E-923.4040535
TGGGGGG39550.022.6039286
AGAGTAC85900.022.37177510-11
ATGGGGA38250.020.7146055
Produced by FastQC (version 0.11.5)