##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921615_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2024098
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.808468759911822	33.0	31.0	34.0	30.0	34.0
2	31.9759562037016	33.0	31.0	34.0	30.0	34.0
3	32.0490248001826	34.0	31.0	34.0	30.0	34.0
4	35.45364601911567	37.0	35.0	37.0	35.0	37.0
5	35.31280797668887	37.0	35.0	37.0	33.0	37.0
6	35.34463499296971	37.0	35.0	37.0	33.0	37.0
7	35.229394525363894	37.0	35.0	37.0	33.0	37.0
8	35.26122747021142	37.0	36.0	37.0	33.0	37.0
9	36.999203595873325	39.0	38.0	39.0	34.0	39.0
10-11	36.948743341478526	39.0	38.0	39.0	34.0	39.0
12-13	36.88318475686454	39.0	38.0	39.0	34.0	39.0
14-15	38.30713483240436	41.0	39.0	41.0	34.0	41.0
16-17	38.31478861201384	41.0	39.0	41.0	34.0	41.0
18-19	38.32482122901164	41.0	39.0	41.0	34.0	41.0
20-21	38.25971692082103	41.0	39.0	41.0	34.0	41.0
22-23	38.26766589364745	41.0	39.0	41.0	34.0	41.0
24-25	38.23042708406411	41.0	39.0	41.0	34.0	41.0
26-27	38.16227079914115	41.0	39.0	41.0	34.0	41.0
28-29	38.096958250045205	41.0	39.0	41.0	34.0	41.0
30-31	37.994621060837964	41.0	38.5	41.0	33.5	41.0
32-33	37.888672386416076	41.0	38.0	41.0	33.0	41.0
34-35	37.76557879114549	41.0	38.0	41.0	33.0	41.0
36-37	37.695631090984726	41.0	38.0	41.0	33.0	41.0
38-39	37.616885150817794	40.0	38.0	41.0	33.0	41.0
40-41	37.50590040600801	40.0	38.0	41.0	33.0	41.0
42-43	37.35947073708882	40.0	38.0	41.0	32.0	41.0
44-45	37.22019808329439	40.0	37.5	41.0	32.0	41.0
46-47	37.04097701791119	40.0	37.0	41.0	31.0	41.0
48-49	36.86135379808685	40.0	37.0	41.0	31.0	41.0
50-51	36.62788066585709	40.0	36.5	41.0	30.5	41.0
52-53	36.50152685294882	40.0	36.0	41.0	30.0	41.0
54-55	36.375879280548666	40.0	35.5	41.0	30.0	41.0
56-57	36.17599567807488	39.0	35.0	41.0	30.0	41.0
58-59	35.983552426809375	39.0	35.0	41.0	30.0	41.0
60-61	35.73398200087149	39.0	35.0	41.0	30.0	41.0
62-63	35.37726261277863	38.0	35.0	41.0	29.0	41.0
64-65	35.09156819482061	37.0	35.0	40.0	29.0	41.0
66-67	34.78628999188774	37.0	35.0	39.5	29.0	41.0
68-69	34.4156135226654	36.0	35.0	39.0	29.0	41.0
70-71	34.03916905209135	36.0	35.0	39.0	28.0	41.0
72-73	33.71638700300085	35.0	34.5	37.5	28.0	40.0
74-75	33.394323792622686	35.0	34.0	37.0	28.0	39.0
76-77	33.066734169985835	35.0	34.0	37.0	27.0	39.0
78-79	32.80229786304813	35.0	34.0	36.0	27.0	38.5
80-81	32.55302559461054	35.0	34.0	36.0	27.0	37.0
82-83	32.32968166561105	35.0	34.0	35.0	26.0	37.0
84-85	32.115856297471765	35.0	34.0	35.0	26.0	36.5
86-87	31.991549816263838	35.0	34.0	35.0	26.0	36.0
88-89	31.897723825625043	35.0	34.0	35.0	26.0	36.0
90-91	31.793688102058297	35.0	34.0	35.0	25.5	36.0
92-93	31.714056582240584	35.0	34.0	35.0	25.0	35.0
94-95	31.648940416916574	35.0	33.5	35.0	25.0	35.0
96-97	31.581894009084543	35.0	33.0	35.0	25.0	35.0
98-99	31.50845191290145	35.0	33.0	35.0	25.0	35.0
100-101	31.440335151756486	35.0	33.0	35.0	24.0	35.0
102-103	30.746467068294123	34.0	31.5	35.0	23.5	35.0
104-105	31.272359095261198	35.0	33.0	35.0	24.0	35.0
106-107	31.374125165876357	35.0	33.0	35.0	24.0	35.0
108-109	31.360337048897833	35.0	33.0	35.0	24.0	35.0
110-111	31.295417761392976	35.0	33.0	35.0	24.0	35.0
112-113	31.25766217841231	35.0	33.0	35.0	24.0	35.0
114-115	31.128323085147063	35.0	33.0	35.0	23.0	35.0
116-117	31.31346629461617	35.0	33.5	35.0	24.0	35.0
118-119	31.2959995020004	35.0	34.0	35.0	24.0	35.0
120-121	31.218768063601665	35.0	34.0	35.0	23.0	35.0
122-123	31.081401196977616	35.0	34.0	35.0	20.0	35.0
124-125	30.43092330509689	34.5	32.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30007.0
3	8296.0
4	4327.0
5	3448.0
6	3679.0
7	4234.0
8	4465.0
9	4882.0
10	5156.0
11	5454.0
12	6035.0
13	7489.0
14	9432.0
15	8669.0
16	5479.0
17	4908.0
18	4398.0
19	4173.0
20	3985.0
21	4023.0
22	4399.0
23	5592.0
24	7396.0
25	9375.0
26	11746.0
27	13898.0
28	16774.0
29	20613.0
30	24857.0
31	31633.0
32	40858.0
33	56173.0
34	87210.0
35	154163.0
36	362185.0
37	764862.0
38	279445.0
39	380.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73942792308198	17.629196154000105	11.35916078649581	27.272215136422105
2	15.729024780306187	19.471440421685646	37.718267231272485	27.081267566735683
3	18.596550878885402	23.497582373267335	28.517370143990135	29.388496603857135
4	13.348330976136724	14.93842421819096	34.19502148199541	37.51822332367691
5	14.658045808030067	37.25800162975343	32.83256383736266	15.25138872485384
6	34.83137734947955	33.737730399538826	16.511443716807683	14.919448534173934
7	30.50313439490509	31.44622098052533	19.384361539427335	18.666283085142247
8	28.204446364413265	34.5902319169664	18.510306294889087	18.695015423731242
9	28.139690503584035	15.138261237432143	18.292778153380223	38.42927010560359
10-11	26.958215233555162	24.823063524106445	26.370523877309545	21.848197365028852
12-13	26.74594294253545	22.18640775451197	26.85598159694957	24.211667706003016
14-15	24.25694596008818	23.53466053054438	24.04487255698484	28.163520952382598
16-17	24.499364203351124	26.653633556222587	24.76786934595123	24.07913289447506
18-19	24.464945160091414	25.930687021089838	26.46065127827992	23.14371654053883
20-21	25.179370302632876	24.89895518250332	26.207064909342577	23.714609605521233
22-23	25.06194941232358	24.41996303909452	26.061884937010582	24.456202611571317
24-25	24.466724308300396	24.808745059288537	26.156768774703558	24.56776185770751
26-27	24.29584084208342	25.024165926599316	26.254336959354553	24.42565627196271
28-29	24.35809611518981	25.48838351411822	26.090977834222702	24.062542536469273
30-31	24.878411974419578	24.991773910500147	26.02514861307637	24.104665502003904
32-33	24.5206826356773	24.963709039883	26.040036167437446	24.475572157002254
34-35	24.5712008747055	25.175648858903095	26.148032443748022	24.10511782264338
36-37	24.583762359409555	25.195458021980627	26.06931249765312	24.151467120956696
38-39	24.667659065902985	25.259467276269227	26.03360784881409	24.039265809013706
40-41	24.625183082294335	25.08627008161722	26.044165044689493	24.244381791398954
42-43	24.49660798638748	24.99514579655626	25.918630210487155	24.58961600656911
44-45	24.384603659929926	25.111999725296513	25.95965267795141	24.54374393682215
46-47	24.426216508452693	25.09961094357978	25.85227911370531	24.621893434262212
48-49	24.315648927090578	25.373205487505885	25.816573433742455	24.49457215166109
50-51	24.380640561799584	25.35476839452449	25.774443237303903	24.490147806372022
52-53	24.53977788394414	25.329742890216135	25.651692104598787	24.47878712124094
54-55	24.270896327653467	25.022233289459646	25.89101142692264	24.815858955964252
56-57	24.39666356731312	25.23961527174348	25.589972323200982	24.773748837742417
58-59	24.341280326153438	25.30428669250889	25.84107430642495	24.513358674912723
60-61	24.65374666023746	25.128995857903973	25.815895095884777	24.401362385973787
62-63	24.489921952099166	25.177790870161836	25.86106510249731	24.471222075241684
64-65	24.26414136074439	24.93014172238696	25.90277249421718	24.902944422651473
66-67	24.28579192450686	25.311001593359766	25.69407492496387	24.7091315571695
68-69	24.275777875983994	25.21392994515301	25.755977205508362	24.75431497335463
70-71	24.19579590410047	25.05017668368248	25.96253391943817	24.79149349277888
72-73	24.297850822619065	25.340214574367725	25.622297039085183	24.739637563928028
74-75	24.204739153849573	25.324309902716912	25.617246132638993	24.853704810794518
76-77	24.111357563388175	24.989346888290914	25.70461842344816	25.19467712487275
78-79	24.03454287920565	25.026122811550657	25.858816763854232	25.080517545389462
80-81	24.1639485835172	25.359098225481176	25.43273596436536	25.044217226636263
82-83	24.219657862908008	25.33629218412794	25.507439782132664	24.93661017083139
84-85	24.15901785617239	25.32864605926103	25.448405236598482	25.063930847968095
86-87	23.88592350765625	25.41112139827222	25.613013796762807	25.089941297308727
88-89	23.869570050516902	25.146153181082713	25.64376320047429	25.340513567926088
90-91	24.02580335342808	24.934908634943913	25.784851176683905	25.254436834944112
92-93	23.989821639070836	25.239736415924526	25.67274410626363	25.097697838741013
94-95	23.960730828907963	25.029588239900534	25.66070521170506	25.34897571948644
96-97	23.736762406345647	25.00996756527088	25.74030478270214	25.51296524568134
98-99	23.73971448827495	24.995096549799044	25.635336460672352	25.62985250125365
100-101	23.668814454636088	25.03984490869513	25.782990744519285	25.50834989214949
102-103	23.55558937081953	24.970955789808603	25.844690609685095	25.62876422968678
104-105	23.681585575401982	25.053727635717244	25.684601239663294	25.58008554921748
106-107	23.596522864708906	24.940491482550097	25.79584304968183	25.66714260305917
108-109	23.556727465680126	24.910303421957803	25.811888662609444	25.721080449752627
110-111	23.41919553222772	25.200015216669563	25.724422161794696	25.65636708930802
112-113	23.356892868505977	25.240354687435158	25.785339796807655	25.617412647251214
114-115	23.303639448287583	25.25705277116029	25.694062244021783	25.745245536530348
116-117	23.148933836580156	25.114096370333534	25.811705355062962	25.925264438023344
118-119	23.2444304570454	24.980608129072294	25.799933203033138	25.97502821084917
120-121	23.03374769550618	25.093445788596984	25.91655428798274	25.9562522279141
122-123	23.026094277133954	25.21354927177469	25.69164668418422	26.068709766907133
124-125	22.807617961463105	25.136048273367077	25.70336221667377	26.352971548496047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	18.5
2	21.5
3	40.5
4	45.5
5	67.5
6	154.5
7	370.5
8	653.5
9	775.0
10	649.0
11	508.0
12	516.5
13	570.5
14	612.0
15	632.5
16	672.5
17	734.0
18	808.0
19	897.0
20	942.5
21	987.0
22	1094.0
23	1276.5
24	1464.0
25	1768.0
26	2335.5
27	3206.5
28	4299.0
29	5764.0
30	7710.5
31	9947.5
32	12883.0
33	16075.0
34	19312.5
35	22850.5
36	27196.0
37	31663.5
38	35438.5
39	39977.5
40	44411.5
41	48184.5
42	52475.5
43	58819.0
44	65873.0
45	72000.5
46	76393.5
47	81156.5
48	87559.5
49	91792.0
50	93808.5
51	94323.0
52	93165.5
53	91795.0
54	91396.5
55	89065.0
56	83154.5
57	75535.0
58	68824.0
59	62209.5
60	53603.0
61	44859.0
62	36900.5
63	28331.0
64	20534.0
65	15308.0
66	11531.5
67	8910.5
68	7025.5
69	5283.5
70	3936.5
71	3049.5
72	2310.5
73	1778.0
74	1374.5
75	966.5
76	638.5
77	364.0
78	195.0
79	113.5
80	71.5
81	45.5
82	23.5
83	14.0
84	9.0
85	5.0
86	3.0
87	5.5
88	4.5
89	1.0
90	1.5
91	2.0
92	1.0
93	0.5
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02415890930182234
2	0.00153154639745704
3	0.030927356284132484
4	0.16481415425537696
5	0.023664862076836202
6	0.0699570870580377
7	0.05236900584853105
8	0.04530413053122922
9	0.033694020744054885
10-11	0.0626204857669935
12-13	0.057927037129625145
14-15	0.059952630752068324
16-17	0.05165263737230114
18-19	0.037251160763955106
20-21	0.02425771874681957
22-23	0.0033348187686564586
24-25	0.004841662804864191
26-27	0.003483032936152301
28-29	0.004915769888612112
30-31	0.0024702361249307097
32-33	0.008794040604753327
34-35	0.005063984056107955
36-37	0.006620232814814303
38-39	0.0023220219574348676
40-41	0.003779461271143986
42-43	0.0039029730773905216
44-45	0.004816960443614885
46-47	0.004866365166113498
48-49	0.002247914873686946
50-51	0.00153154639745704
52-53	0.0010374991724708981
54-55	0.005236900584853105
56-57	9.88094449972284E-4
58-59	9.88094449972284E-4
60-61	9.880944499722839E-5
62-63	0.001012796811221591
64-65	0.0
66-67	0.003606544742398836
68-69	6.175590312326774E-4
70-71	2.4702361249307098E-5
72-73	0.0021985101511883317
74-75	0.005335710029850334
76-77	0.002050295983692489
78-79	2.47023612493071E-4
80-81	0.0
82-83	0.005632138364842019
84-85	0.0017785700099501112
86-87	0.0
88-89	0.0011363086174681265
90-91	9.88094449972284E-4
92-93	0.0
94-95	0.0033348187686564586
96-97	0.0016303558424542685
98-99	6.422613924819846E-4
100-101	0.0
102-103	0.0014080345912105047
104-105	0.0
106-107	8.892850049750556E-4
108-109	0.0025690455699279384
110-111	9.880944499722839E-5
112-113	2.964283349916852E-4
114-115	0.0
116-117	0.002050295983692489
118-119	0.002667855014925167
120-121	0.0031371998786620015
122-123	0.0016550582037035758
124-125	0.10441688100082111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2024098.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.053007741179165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1372589093853	45.94155904082244
2	11.138743169508707	12.93275086892991
3	3.598654651301083	6.2673817898943645
4	1.8092389710696222	4.201270559725912
5	1.0233740458486102	2.97049707028856
6	0.6897495614199726	2.4025221977153164
7	0.46970207149879034	1.9087332594937014
8	0.36040339997202825	1.673800109481876
9	0.25135041160719146	1.3132482651702768
>10	1.4251482297464302	14.681558041281061
>50	0.07143549723042239	2.8088988529540324
>100	0.023660634286048973	2.218567172563126
>500	9.389945589774153E-4	0.3376737068600502
>1k	3.414525669008729E-4	0.34153906481940643
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2418	0.11946061900164912	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.9404722498614196E-5
2	0.0	0.0	0.0	0.0	9.880944499722839E-5
3	0.0	0.0	0.0	0.0	9.880944499722839E-5
4	0.0	0.0	0.0	0.0	9.880944499722839E-5
5	0.0	0.0	0.0	0.0	9.880944499722839E-5
6	0.0	0.0	0.0	0.0	9.880944499722839E-5
7	0.0	0.0	0.0	0.0	9.880944499722839E-5
8	0.0	0.0	0.0	0.0	9.880944499722839E-5
9	0.0	0.0	0.0	0.0	9.880944499722839E-5
10-11	0.0	0.0	0.0	0.0	9.880944499722839E-5
12-13	0.0	0.0	0.0	0.0	4.446425024875278E-4
14-15	0.0	0.0	0.0	0.0	5.681543087340632E-4
16-17	0.0	0.0	0.0	0.0	6.422613924819846E-4
18-19	0.0	0.0	0.0	7.410708374792129E-5	7.41070837479213E-4
20-21	0.0	0.0	0.0	1.9761888999445678E-4	8.398802824764414E-4
22-23	0.0	0.0	0.0	2.717259737423781E-4	0.0010374991724708981
24-25	0.0	0.0	0.0	4.199401412382207E-4	0.0011116062562188194
26-27	0.0	0.0	0.0	0.0011610109787174336	0.0011857133399667407
28-29	0.0	0.0	0.0	0.003952377799889136	0.0011857133399667407
30-31	0.0	0.0	0.0	0.013586298687118904	0.0011857133399667407
32-33	2.4702361249307098E-5	0.0	0.0	0.03507735297401608	0.0011857133399667407
34-35	4.9404722498614196E-5	0.0	0.0	0.06141007006577744	0.0012351180624653548
36-37	4.9404722498614196E-5	0.0	0.0	0.10145259765090425	0.0012351180624653548
38-39	4.9404722498614196E-5	0.0	0.0	0.1663704030140833	0.0012845227849639692
40-41	4.9404722498614196E-5	0.0	0.0	0.23714266799334816	0.0012845227849639692
42-43	4.9404722498614196E-5	0.0	0.0	0.3076679093601199	0.0012845227849639692
44-45	4.9404722498614196E-5	0.0	0.0	0.3855050496566866	0.0012845227849639692
46-47	4.9404722498614196E-5	0.0	0.0	0.47332194389797333	0.0012845227849639692
48-49	4.9404722498614196E-5	0.0	0.0	0.5646465734366617	0.0013833322299611976
50-51	4.9404722498614196E-5	0.0	0.0	0.6571322139540674	0.0013833322299611976
52-53	4.9404722498614196E-5	0.0	0.0	0.7565345156212793	0.001506844036207733
54-55	4.9404722498614196E-5	0.0	0.0	0.8651013933119839	0.0015809511199556543
56-57	4.9404722498614196E-5	0.0	0.0	0.982462311607442	0.0015809511199556543
58-59	4.9404722498614196E-5	0.0	0.0	1.1122485176113015	0.0015809511199556543
60-61	4.9404722498614196E-5	0.0	0.0	1.24099722444269	0.0015809511199556543
62-63	4.9404722498614196E-5	0.0	0.0	1.3815289575899983	0.0015809511199556543
64-65	4.9404722498614196E-5	0.0	0.0	1.523493427689766	0.0015809511199556543
66-67	4.9404722498614196E-5	0.0	0.0	1.6807733617641043	0.0016550582037035755
68-69	4.9404722498614196E-5	0.0	0.0	1.8534922716192594	0.0017291652874514968
70-71	4.9404722498614196E-5	0.0	0.0	2.042218311563966	0.0018032723711994183
72-73	7.410708374792129E-5	0.0	0.0	2.2173827551828023	0.002000891261193875
74-75	9.880944499722839E-5	0.0	0.0	2.401859988992628	0.002050295983692489
76-77	9.880944499722839E-5	0.0	0.0	2.6186726136777962	0.0020997007061911033
78-79	9.880944499722839E-5	0.0	0.0	2.858903076827308	0.0021244030674404104
80-81	1.4821416749584258E-4	0.0	0.0	3.0953046739831764	0.0021738077899390246
82-83	1.4821416749584258E-4	0.0	0.0	3.3368196599176523	0.0023714266799334813
84-85	1.4821416749584258E-4	0.0	0.0	3.5984670702703134	0.0023714266799334813
86-87	1.4821416749584258E-4	0.0	0.0	3.892894514000804	0.0023961290411827884
88-89	1.4821416749584258E-4	0.0	0.0	4.218842170685411	0.0024949384861800167
90-91	1.4821416749584258E-4	0.0	0.0	4.556350532434694	0.0025196408474293243
92-93	1.4821416749584258E-4	0.0	0.0	4.89269788320526	0.0026184502924265526
94-95	1.4821416749584258E-4	0.0	0.0	5.2361101092931275	0.002667855014925167
96-97	1.4821416749584258E-4	0.0	0.0	5.621639861311063	0.0027913668211717022
98-99	1.4821416749584258E-4	0.0	0.0	6.049855293567802	0.0029148786274182377
100-101	1.4821416749584258E-4	0.0	0.0	6.499018328163952	0.0029148786274182377
102-103	1.4821416749584258E-4	0.0	0.0	6.957345938783597	0.003013688072415466
104-105	1.4821416749584258E-4	0.0	0.0	7.439313709118828	0.003013688072415466
106-107	1.4821416749584258E-4	0.0	0.0	7.956185915899329	0.00306309279491408
108-109	1.4821416749584258E-4	0.0	0.0	8.505245299387678	0.00306309279491408
110-111	1.4821416749584258E-4	0.0	0.0	9.099312385072265	0.0031124975174126944
112-113	1.4821416749584258E-4	0.0	0.0	9.6897976283757	0.0031124975174126944
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2900	0.0	49.771317	1
GTATCAA	4785	0.0	48.36281	1
TCAACGC	6135	0.0	37.029095	4
CAACGCA	6225	0.0	36.36643	5
GTACATG	10830	0.0	35.96222	1
ATCAACG	6525	0.0	35.093475	3
TATCAAC	6560	0.0	34.997143	2
AACGCAG	6510	0.0	34.788982	6
TACATGG	11285	0.0	34.02985	2
ACATGGG	11475	0.0	33.31049	3
CATGGGG	10140	0.0	32.223694	4
ATGGGGG	5810	0.0	30.248392	5
ACGCAGA	7830	0.0	29.074968	7
CGCAGAG	8240	0.0	27.76949	8
GTATTAT	560	0.0	24.49636	1
GCAGAGT	9535	0.0	23.747988	9
TATACCG	280	2.57387E-9	23.404053	5
TGGGGGG	3955	0.0	22.603928	6
AGAGTAC	8590	0.0	22.371775	10-11
ATGGGGA	3825	0.0	20.714605	5
>>END_MODULE