FastQCFastQC Report
Mon 15 Aug 2016
SRR2921614_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921614_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837083
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22650.046.5159571
GTATCAA33900.043.194881
TCAACGC40250.036.0727124
GTACATG97200.035.5800671
CAACGCA41500.035.2729575
TACATGG101000.034.0543942
AACGCAG43450.033.9610636
ACATGGG102500.032.916533
CATGGGG87000.032.7620544
TATCAAC44250.032.6783182
ATCAACG44900.032.4694253
ATGGGGG53550.029.113655
ACGCAGA53550.027.4393627
CGCAGAG57600.025.5086428
GCAGAGT65700.022.363139
ATGGGGA34000.021.5268785
TGGGGGG37700.021.464316
AGAGTAC56450.020.81097210-11
GCCTAGT4600.020.6974564
CAGAGTA61300.020.52291710-11
Produced by FastQC (version 0.11.5)