##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921614_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1837083
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.24872420026749	31.0	31.0	34.0	27.0	34.0
2	30.230984664274832	31.0	31.0	34.0	26.0	34.0
3	30.44523845683619	31.0	31.0	34.0	27.0	34.0
4	33.91416065577875	37.0	35.0	37.0	30.0	37.0
5	33.956364519186124	37.0	35.0	37.0	30.0	37.0
6	33.86157076190896	37.0	35.0	37.0	30.0	37.0
7	33.891061536141805	37.0	35.0	37.0	30.0	37.0
8	33.89709065948572	37.0	35.0	37.0	30.0	37.0
9	35.54570969302966	39.0	37.0	39.0	30.0	39.0
10-11	35.569103573436806	39.0	37.0	39.0	30.0	39.0
12-13	35.505384623340376	39.0	37.0	39.0	30.0	39.0
14-15	36.80874489612064	40.0	38.0	41.0	30.0	41.0
16-17	36.7685202573863	40.0	38.0	41.0	30.0	41.0
18-19	36.7166951629295	40.0	38.0	41.0	29.5	41.0
20-21	36.77390923545643	40.0	38.0	41.0	30.0	41.0
22-23	36.695484090811355	40.0	38.0	41.0	29.5	41.0
24-25	36.67498610569038	40.0	38.0	41.0	29.5	41.0
26-27	36.63521898575078	40.0	38.0	41.0	29.5	41.0
28-29	36.59254862191855	40.0	38.0	41.0	29.5	41.0
30-31	36.49649770859564	40.0	38.0	41.0	29.0	41.0
32-33	36.41146045116089	40.0	37.5	41.0	29.0	41.0
34-35	36.28218403850016	40.0	37.0	41.0	28.5	41.0
36-37	36.179194407656055	40.0	37.0	41.0	28.0	41.0
38-39	36.064115774845234	40.0	37.0	41.0	27.0	41.0
40-41	35.95834020564122	40.0	36.5	41.0	27.0	41.0
42-43	35.70670377985099	40.0	36.0	41.0	26.5	41.0
44-45	35.339991442956034	40.0	35.0	41.0	25.0	41.0
46-47	35.08372675594951	39.0	35.0	41.0	24.0	41.0
48-49	34.89484497978589	39.0	35.0	41.0	24.0	41.0
50-51	34.685809786493046	39.0	35.0	40.5	23.5	41.0
52-53	34.47237060056622	38.5	34.0	40.0	23.0	41.0
54-55	34.23100289970567	38.0	34.0	40.0	22.5	41.0
56-57	33.966727687317345	38.0	34.0	40.0	21.5	41.0
58-59	33.96771539445959	38.0	34.0	40.0	22.0	41.0
60-61	33.75215110041299	37.0	34.0	40.0	21.5	41.0
62-63	33.44589983141752	37.0	34.0	40.0	20.0	41.0
64-65	33.14396736565523	36.0	33.0	39.5	19.5	41.0
66-67	32.81968833199153	36.0	33.0	39.0	18.5	41.0
68-69	32.483467812831535	35.0	33.0	39.0	18.0	40.5
70-71	32.1191737118029	35.0	33.0	38.0	15.5	40.0
72-73	31.775574375245974	35.0	32.0	37.0	13.0	39.0
74-75	31.41672123687389	35.0	32.0	37.0	10.0	39.0
76-77	31.121615898682858	35.0	32.0	36.0	8.0	39.0
78-79	30.84396186780891	35.0	32.0	36.0	7.0	37.0
80-81	30.585752521796785	35.0	31.5	35.0	7.0	37.0
82-83	30.364798160997623	35.0	31.0	35.0	3.5	36.5
84-85	30.14406099234493	35.0	31.0	35.0	2.0	36.0
86-87	29.969014192608608	35.0	31.0	35.0	2.0	36.0
88-89	29.801844282484787	34.5	31.0	35.0	2.0	35.5
90-91	29.61747455068715	34.0	31.0	35.0	2.0	35.0
92-93	29.53273450355809	34.0	31.0	35.0	2.0	35.0
94-95	29.40531375011363	34.0	30.0	35.0	2.0	35.0
96-97	29.312647822662342	34.0	30.0	35.0	2.0	35.0
98-99	29.205172275830762	34.0	30.0	35.0	2.0	35.0
100-101	29.0900811231719	34.0	30.0	35.0	2.0	35.0
102-103	28.190988921023163	33.5	28.0	34.5	2.0	35.0
104-105	29.12410653193133	34.0	30.5	35.0	2.0	35.0
106-107	29.40465836328571	34.5	31.0	35.0	2.0	35.0
108-109	29.395251602676634	35.0	31.0	35.0	2.0	35.0
110-111	29.321985724107186	35.0	31.0	35.0	2.0	35.0
112-113	29.22432764333457	35.0	31.0	35.0	2.0	35.0
114-115	29.105522450537073	35.0	31.0	35.0	2.0	35.0
116-117	28.976048714184387	34.5	31.0	35.0	2.0	35.0
118-119	28.669212822719494	34.0	30.0	35.0	2.0	35.0
120-121	28.533660700142562	34.0	30.0	35.0	2.0	35.0
122-123	28.265870676501823	34.0	30.0	35.0	2.0	35.0
124-125	27.466859962233606	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69496.0
3	12658.0
4	6289.0
5	5457.0
6	5831.0
7	6263.0
8	6451.0
9	7100.0
10	7413.0
11	7513.0
12	7260.0
13	7295.0
14	7079.0
15	6612.0
16	6012.0
17	5714.0
18	5425.0
19	5079.0
20	5055.0
21	5541.0
22	6161.0
23	8218.0
24	11612.0
25	14648.0
26	16383.0
27	18643.0
28	21752.0
29	26705.0
30	32504.0
31	40717.0
32	53320.0
33	73125.0
34	111786.0
35	184490.0
36	361472.0
37	528010.0
38	131928.0
39	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.460510231473435	17.036011759075087	11.8862708605698	25.617207148881672
2	16.893874931200564	19.606328404816693	37.81483737746078	25.684959286521952
3	20.13837511316676	22.8697837814002	28.495974992528133	28.495866112904906
4	14.476575293543778	14.807955297724817	33.80604116581304	36.909428242918366
5	15.076653707302015	36.96527648852576	32.36676181573384	15.59130798843838
6	34.80191333125923	32.84662783266034	16.612952526871144	15.738506309209283
7	30.542425982067282	31.07565793415375	19.61639979651128	18.765516287267683
8	27.706184584450256	34.88562456362402	18.64813582362187	18.76005502830385
9	27.906366417184742	15.926333682891158	18.04447987211193	38.12282002781217
10-11	26.85569149527152	25.052692978844384	26.173487616196866	21.91812790968723
12-13	26.568919378358167	22.602799201130416	26.34263469627685	24.485646724234563
14-15	24.397172967866467	24.0398735676307	23.40547928050072	28.157474184002112
16-17	24.92636628722185	27.001042769901794	24.092803798622892	23.979787144253468
18-19	24.85094963110442	26.314278992665653	25.409166951661284	23.42560442456864
20-21	25.508894491241634	25.130272851518896	25.304650618285574	24.056182038953896
22-23	25.72135320944796	24.76430467633245	25.00132053638282	24.51302157783677
24-25	25.09235590668058	25.265156537442362	24.949421715487997	24.693065840389064
26-27	24.63065830174705	25.543665316080688	25.241390014377206	24.58428636779506
28-29	24.794901125855244	25.734806590720154	25.21039499828046	24.25989728514414
30-31	25.291054121746704	25.233828814113036	25.162229119024282	24.312887945115975
32-33	24.86196073071718	25.324464770276684	25.093975059957003	24.71959943904914
34-35	24.928208845410893	25.670572679115732	25.09066134997323	24.310557125500143
36-37	24.992773217298332	25.694955188503343	25.054806504594385	24.25746508960394
38-39	24.922149503986375	25.7607488674621	25.16337102516016	24.153730603391367
40-41	24.901152945616353	25.481533074860184	25.25300244268831	24.364311536835146
42-43	24.910803781386097	25.254439938127526	25.141954427578877	24.6928018529075
44-45	24.706008532285708	25.365447727228187	25.325350557603816	24.603193182882293
46-47	24.597214818944718	25.431070389606287	25.33671376512333	24.63500102632566
48-49	24.537057959706388	25.62432993260803	25.378763335327996	24.45984877235759
50-51	24.525113002863765	25.585568956453297	25.467958314650392	24.42135972603254
52-53	24.712614264176544	25.42636211827775	25.321006858709065	24.54001675883664
54-55	24.546277169740847	25.240894781602165	25.401700010998024	24.811128037658968
56-57	24.62767425792116	25.5379278690167	25.142829452404975	24.69156842065717
58-59	24.555957712539787	25.6118920143165	25.363438485770068	24.46871178737365
60-61	24.77413699465475	25.440721084676	25.448152982763713	24.336988937905538
62-63	24.591245271348892	25.459035779854478	25.478282506005414	24.471436442791216
64-65	24.60145504535958	25.20484289185161	25.362570353280656	24.831131709508156
66-67	24.55554351619787	25.49991315327657	25.186557107301716	24.75798622322385
68-69	24.409580861145027	25.5449159013602	25.351414847149684	24.694088390345094
70-71	24.279331127138835	25.358983135491986	25.61791554103639	24.743770196332793
72-73	24.37207352396716	25.520395496221443	25.39209334233508	24.715437637476313
74-75	24.293712571345978	25.500602505891003	25.378658688782775	24.82702623398024
76-77	24.409681163586317	25.2433574839776	25.292872837820475	25.054088514615604
78-79	24.27185628970737	25.315937068760636	25.4990432254125	24.913163416119495
80-81	24.339216312383982	25.557462013529953	25.350056445130082	24.753265228955986
82-83	24.386143061321718	25.48933505751038	25.47466140338937	24.649860477778535
84-85	24.415161619598504	25.43564477713508	25.288873766587272	24.860319836679142
86-87	24.21372442428352	25.61111817360468	25.33811272084546	24.837044681266338
88-89	24.184904151288976	25.408368022462387	25.379048664417486	25.027679161831152
90-91	24.37851752357629	25.25993672336809	25.464107580074447	24.897438172981172
92-93	24.249767617201908	25.364932857362554	25.601439344347078	24.783860181088464
94-95	24.31972414146025	25.197515247972994	25.537084959389095	24.94567565117766
96-97	24.20257572321932	25.15436454995619	25.544525832770987	25.098533894053503
98-99	24.133937373806656	25.26787420323654	25.399722258967657	25.198466163989146
100-101	24.040585725313598	25.35516320413392	25.524687762330274	25.079563308222212
102-103	23.8193962455192	25.222958021344528	25.767621753887443	25.19002397924883
104-105	23.836821125719755	25.306062457726654	25.70924644903858	25.147869967515014
106-107	23.927562735242702	25.18780631392838	25.70504426761049	25.179586683218425
108-109	23.880072908772572	25.278253238212212	25.6484862936734	25.19318755934182
110-111	23.687423620958544	25.614475342327804	25.629635476346653	25.068465560367
112-113	23.574039134652338	25.587901637523803	25.75724997033274	25.080809257491122
114-115	23.560049519989285	25.602912402536106	25.72325534043904	25.11378273703557
116-117	23.464276226684607	25.489198599443437	25.780627376177474	25.265897797694482
118-119	23.606222775629565	25.375713029786013	25.690307271177453	25.327756923406973
120-121	23.462176813943575	25.530888740221474	25.75845331211103	25.24848113372392
122-123	23.373136018272657	25.589011149324303	25.741401215636706	25.296451616766337
124-125	23.20980548643953	25.45113051911511	25.870295351207545	25.468768643237816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	485.0
1	392.0
2	261.5
3	201.5
4	164.0
5	141.5
6	140.5
7	149.0
8	154.5
9	165.5
10	167.0
11	186.5
12	228.0
13	250.5
14	262.0
15	288.0
16	343.5
17	385.0
18	425.0
19	476.0
20	537.0
21	631.5
22	744.5
23	961.5
24	1201.0
25	1511.0
26	2051.0
27	2861.5
28	3924.0
29	5328.5
30	7122.0
31	9196.0
32	11931.0
33	14642.5
34	17188.0
35	20347.5
36	24225.0
37	28348.0
38	32337.0
39	36267.0
40	40284.5
41	44389.5
42	48115.0
43	53210.5
44	58949.5
45	64116.0
46	68686.0
47	73130.5
48	78595.5
49	82352.0
50	83571.5
51	83726.5
52	83420.0
53	83106.5
54	82428.0
55	79819.0
56	74953.5
57	68741.5
58	62839.0
59	56549.5
60	49001.5
61	41552.0
62	34406.5
63	27023.5
64	20327.5
65	15541.5
66	11992.0
67	9263.0
68	7191.5
69	5576.5
70	4310.5
71	3383.0
72	2560.5
73	1953.0
74	1580.0
75	1203.5
76	858.5
77	567.5
78	343.0
79	231.5
80	165.5
81	127.5
82	104.5
83	68.5
84	57.5
85	52.5
86	46.0
87	36.0
88	25.5
89	21.5
90	24.0
91	20.5
92	17.5
93	20.0
94	15.5
95	11.5
96	9.5
97	7.0
98	7.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029612162324728932
2	0.012084375066341586
3	0.010451351408727858
4	0.011921072700580213
5	0.0172011825268646
6	0.09525971336080079
7	0.06036744120978747
8	0.05100477223946877
9	0.02596507615605827
10-11	0.05307326887244616
12-13	0.01292810395610868
14-15	0.028741216374001607
16-17	0.03418462856604737
18-19	0.04305739043908196
20-21	0.030102069422013048
22-23	0.038539358319683976
24-25	0.03121796892138243
26-27	0.04588796477894575
28-29	0.04542527474262186
30-31	0.026291680887581017
32-33	0.03040145709257557
34-35	0.046486740120070785
36-37	0.00903606423879596
38-39	0.021202090488018233
40-41	0.0428124368904399
42-43	0.021882517012023952
44-45	0.01605806596653499
46-47	0.023624408913478598
48-49	0.0279791386671152
50-51	0.02800635572807543
52-53	0.02395101364500134
54-55	0.021174873427058004
56-57	0.024604223108046833
58-59	0.017473353136466887
60-61	0.022073036438745555
62-63	0.022154687621626243
64-65	0.02016784217152954
66-67	0.0279791386671152
68-69	0.021800865829143267
70-71	0.04841915144824703
72-73	0.02193695113394441
74-75	0.009335451909358479
76-77	0.01518712001580767
78-79	0.024141533071722943
80-81	0.018698120879677184
82-83	0.024930827839569576
84-85	0.01132229735945518
86-87	0.016684058368620254
88-89	0.02245407529218876
90-91	0.02101157106129663
92-93	0.007375823520222004
94-95	0.01085960732313129
96-97	0.01515990295484744
98-99	0.006532094630454912
100-101	0.009852576067602824
102-103	0.004245861509795692
104-105	0.0043819468145968364
106-107	9.525971336080078E-4
108-109	0.014533910552762177
110-111	0.0015241554137728128
112-113	0.0022317989987387615
114-115	0.013554096358193941
116-117	0.005388978070125302
118-119	0.28303021692541924
120-121	0.0011975506822500671
122-123	0.0011431165603296095
124-125	0.31694267488186434
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1837083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.20711624092795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9074007376862	54.89059917873187
2	9.523665428508654	12.610688481299587
3	3.01008909625262	5.978679560734409
4	1.4276391380768558	3.7807948145901027
5	0.7869200291521004	2.604985292119376
6	0.5009698281107037	1.9900660585753829
7	0.3538810505705587	1.6400610695410676
8	0.2568099045267635	1.360211456066003
9	0.19893868727008032	1.1854041113617038
>10	0.9838653780340955	11.140678399569888
>50	0.040208522191620986	1.7554963150945488
>100	0.009365857494628718	0.9527347387717978
>500	2.4634212519632394E-4	0.10960052354435684
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.088682438409152E-4
2	0.0	0.0	0.0	0.0	1.088682438409152E-4
3	0.0	0.0	0.0	0.0	1.088682438409152E-4
4	0.0	0.0	0.0	1.088682438409152E-4	1.088682438409152E-4
5	0.0	0.0	0.0	1.088682438409152E-4	1.088682438409152E-4
6	0.0	0.0	0.0	1.088682438409152E-4	1.088682438409152E-4
7	0.0	0.0	0.0	1.088682438409152E-4	1.088682438409152E-4
8	0.0	0.0	0.0	1.088682438409152E-4	1.088682438409152E-4
9	0.0	0.0	0.0	1.6330236576137278E-4	1.6330236576137278E-4
10-11	0.0	0.0	0.0	2.72170609602288E-4	1.6330236576137278E-4
12-13	0.0	0.0	0.0	2.72170609602288E-4	1.6330236576137278E-4
14-15	0.0	0.0	0.0	2.72170609602288E-4	2.72170609602288E-4
16-17	0.0	0.0	0.0	2.72170609602288E-4	3.2660473152274557E-4
18-19	0.0	0.0	0.0	2.993876705625168E-4	3.2660473152274557E-4
20-21	0.0	0.0	0.0	4.0825591440343193E-4	3.5382179248297436E-4
22-23	0.0	0.0	0.0	6.259924020852623E-4	4.354729753636608E-4
24-25	0.0	0.0	0.0	8.16511828806864E-4	4.899070972841184E-4
26-27	0.0	0.0	0.0	0.0013880701089716686	5.715582801648048E-4
28-29	0.0	0.0	0.0	0.004055342083074091	5.987753411250336E-4
30-31	0.0	0.0	0.0	0.01603084890557476	5.987753411250336E-4
32-33	0.0	0.0	0.0	0.04098889380610457	7.892947678466351E-4
34-35	0.0	0.0	0.0	0.07049218788699259	8.709459507273216E-4
36-37	0.0	0.0	0.0	0.10832390262171061	8.709459507273216E-4
38-39	0.0	0.0	0.0	0.16716718841772527	8.709459507273216E-4
40-41	0.0	0.0	0.0	0.23363125128260398	8.709459507273216E-4
42-43	0.0	5.44341219204576E-5	0.0	0.302980322609267	8.709459507273216E-4
44-45	0.0	5.44341219204576E-5	0.0	0.38678165330581143	8.709459507273216E-4
46-47	0.0	5.44341219204576E-5	0.0	0.4742300701710266	8.981630116875503E-4
48-49	0.0	5.44341219204576E-5	0.0	0.5583035714771734	9.798141945682368E-4
50-51	0.0	5.44341219204576E-5	0.0	0.6409617856133882	9.798141945682368E-4
52-53	0.0	5.44341219204576E-5	0.0	0.7328738004760809	0.0011158994993693808
54-55	0.0	5.44341219204576E-5	0.0	0.83983685004978	0.0013064189260909823
56-57	0.0	5.44341219204576E-5	0.0	0.9688729360622248	0.0013064189260909823
58-59	0.0	5.44341219204576E-5	0.0	1.0923023075168623	0.0013064189260909823
60-61	0.0	5.44341219204576E-5	0.0	1.2109959103644201	0.0013064189260909823
62-63	0.0	5.44341219204576E-5	0.0	1.3339625917827338	0.0013608530480114399
64-65	0.0	5.44341219204576E-5	0.0	1.474484277520395	0.0013608530480114399
66-67	0.0	5.44341219204576E-5	0.0	1.6408077370483531	0.0013608530480114399
68-69	0.0	5.44341219204576E-5	0.0	1.8206580758735451	0.0013608530480114399
70-71	0.0	1.088682438409152E-4	0.0	1.9953643901772538	0.0014152871699318974
72-73	0.0	1.6330236576137278E-4	0.0	2.165852060032127	0.0015241554137728126
74-75	0.0	1.6330236576137278E-4	0.0	2.3533776100481036	0.0015241554137728126
76-77	0.0	1.6330236576137278E-4	0.0	2.5831984727962753	0.0015241554137728126
78-79	0.0	1.6330236576137278E-4	0.0	2.8353917596537555	0.0015241554137728126
80-81	0.0	1.6330236576137278E-4	0.0	3.0724795776783083	0.0015241554137728126
82-83	0.0	1.6330236576137278E-4	0.0	3.305375968314986	0.0016058065966534992
84-85	0.0	1.6330236576137278E-4	0.0	3.566795838837984	0.0016602407185739567
86-87	0.0	1.6330236576137278E-4	0.0	3.876335473138666	0.001769108962414872
88-89	0.0	1.6330236576137278E-4	0.0	4.220467991919799	0.0018507601452955583
90-91	0.0	1.6330236576137278E-4	0.0	4.564328340091329	0.0018507601452955583
92-93	0.0	1.6330236576137278E-4	0.0	4.885326357056268	0.0018507601452955583
94-95	0.0	1.6330236576137278E-4	0.0	5.228860100496275	0.0019596283891364736
96-97	0.0	1.6330236576137278E-4	0.0	5.624949988650485	0.0020957136939376174
98-99	0.0	1.6330236576137278E-4	0.0	6.091096591716324	0.0021229307548978464
100-101	2.72170609602288E-5	1.6330236576137278E-4	0.0	6.566252041959999	0.0021229307548978464
102-103	5.44341219204576E-5	1.6330236576137278E-4	0.0	7.028261651759882	0.0021229307548978464
104-105	5.44341219204576E-5	1.6330236576137278E-4	0.0	7.502056248955546	0.0021229307548978464
106-107	5.44341219204576E-5	1.6330236576137278E-4	0.0	8.028488642048291	0.0021501478158580754
108-109	5.44341219204576E-5	1.6330236576137278E-4	0.0	8.62337194345601	0.0022045819377785325
110-111	5.44341219204576E-5	1.6330236576137278E-4	0.0	9.246778724750051	0.0023134501816194477
112-113	5.44341219204576E-5	1.6330236576137278E-4	0.0	9.841090467877608	0.0023678843035399057
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2265	0.0	46.515957	1
GTATCAA	3390	0.0	43.19488	1
TCAACGC	4025	0.0	36.072712	4
GTACATG	9720	0.0	35.580067	1
CAACGCA	4150	0.0	35.272957	5
TACATGG	10100	0.0	34.054394	2
AACGCAG	4345	0.0	33.961063	6
ACATGGG	10250	0.0	32.91653	3
CATGGGG	8700	0.0	32.762054	4
TATCAAC	4425	0.0	32.678318	2
ATCAACG	4490	0.0	32.469425	3
ATGGGGG	5355	0.0	29.11365	5
ACGCAGA	5355	0.0	27.439362	7
CGCAGAG	5760	0.0	25.508642	8
GCAGAGT	6570	0.0	22.36313	9
ATGGGGA	3400	0.0	21.526878	5
TGGGGGG	3770	0.0	21.46431	6
AGAGTAC	5645	0.0	20.810972	10-11
GCCTAGT	460	0.0	20.697456	4
CAGAGTA	6130	0.0	20.522917	10-11
>>END_MODULE