FastQCFastQC Report
Mon 15 Aug 2016
SRR2921614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837083
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.13450671526545072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21030.11447495839872232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.059.2149431
GTATCAA43900.049.8868941
TCAACGC56400.038.6093034
CAACGCA57350.037.811015
TATCAAC58400.037.3963742
GTACATG94300.037.145941
ATCAACG59150.037.0210573
AACGCAG60300.036.27546
TACATGG98850.035.192742
ACATGGG98300.034.902123
CATGGGG90100.031.7829844
ACGCAGA72350.030.3152317
ATGGGGG52700.029.6169955
CGCAGAG76450.028.7626848
GCAGAGT85400.025.5389949
AGAGTAC76700.023.07499710-11
CAGAGTA81750.022.67008610-11
ATGGGGA33500.021.6952845
GAGTACT35750.021.64967212-13
TGGGGGG34550.021.0462766
Produced by FastQC (version 0.11.5)