##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921614_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1837083
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87179457868806	33.0	31.0	34.0	30.0	34.0
2	32.034932009060014	33.0	31.0	34.0	30.0	34.0
3	32.105693645850515	34.0	31.0	34.0	30.0	34.0
4	35.51946155943961	37.0	35.0	37.0	35.0	37.0
5	35.3947660503091	37.0	35.0	37.0	33.0	37.0
6	35.43432495973236	37.0	36.0	37.0	33.0	37.0
7	35.32628955795683	37.0	36.0	37.0	33.0	37.0
8	35.364997117713244	37.0	36.0	37.0	33.0	37.0
9	37.10449772819192	39.0	38.0	39.0	34.0	39.0
10-11	37.05783788756414	39.0	38.0	39.0	34.0	39.0
12-13	36.995728010111684	39.0	38.0	39.0	34.0	39.0
14-15	38.42350781102432	41.0	39.0	41.0	34.0	41.0
16-17	38.42915262946748	41.0	39.0	41.0	34.0	41.0
18-19	38.44030726973142	41.0	39.0	41.0	34.0	41.0
20-21	38.38125087434808	41.0	39.0	41.0	34.0	41.0
22-23	38.38739213198315	41.0	39.0	41.0	34.0	41.0
24-25	38.346915735434926	41.0	39.0	41.0	34.0	41.0
26-27	38.275281519670045	41.0	39.0	41.0	34.0	41.0
28-29	38.21002562214119	41.0	39.0	41.0	34.0	41.0
30-31	38.10191755081289	41.0	38.5	41.0	34.0	41.0
32-33	37.990280515360496	41.0	38.0	41.0	33.0	41.0
34-35	37.85961303871409	41.0	38.0	41.0	33.0	41.0
36-37	37.78713917661858	40.5	38.0	41.0	33.0	41.0
38-39	37.709826937596176	40.0	38.0	41.0	33.0	41.0
40-41	37.594511516355	40.0	38.0	41.0	33.0	41.0
42-43	37.43666970953409	40.0	38.0	41.0	32.5	41.0
44-45	37.286615248195105	40.0	37.5	41.0	32.0	41.0
46-47	37.09048965125691	40.0	37.0	41.0	31.5	41.0
48-49	36.90405115065569	40.0	37.0	41.0	31.0	41.0
50-51	36.65743382307713	40.0	36.0	41.0	31.0	41.0
52-53	36.52758040872405	40.0	36.0	41.0	30.5	41.0
54-55	36.40308657801526	40.0	35.5	41.0	30.0	41.0
56-57	36.203731404623525	39.0	35.0	41.0	30.0	41.0
58-59	36.00768773103883	39.0	35.0	41.0	30.0	41.0
60-61	35.75371227102967	39.0	35.0	41.0	30.0	41.0
62-63	35.40313393570133	38.0	35.0	41.0	29.0	41.0
64-65	35.118192264584664	37.0	35.0	40.0	29.0	41.0
66-67	34.815823509335175	37.0	35.0	39.5	29.0	41.0
68-69	34.44761613928168	36.0	35.0	39.0	29.0	41.0
70-71	34.07413981839689	35.5	35.0	39.0	28.5	41.0
72-73	33.752801860340554	35.0	34.5	37.5	28.0	40.0
74-75	33.431405385603156	35.0	34.0	37.0	28.0	39.0
76-77	33.10875665389098	35.0	34.0	36.5	28.0	39.0
78-79	32.84918073924804	35.0	34.0	36.0	27.0	38.0
80-81	32.6003814198923	35.0	34.0	36.0	27.0	37.0
82-83	32.38062842016392	35.0	34.0	35.0	26.5	37.0
84-85	32.16800329653042	35.0	34.0	35.0	26.0	36.0
86-87	32.04321225551595	35.0	34.0	35.0	26.0	36.0
88-89	31.946862226693078	35.0	34.0	35.0	26.0	36.0
90-91	31.84280759225359	35.0	34.0	35.0	26.0	36.0
92-93	31.767020869498005	35.0	34.0	35.0	25.0	35.0
94-95	31.70292686830154	35.0	34.0	35.0	25.0	35.0
96-97	31.640544548068867	35.0	33.0	35.0	25.0	35.0
98-99	31.568141722502467	35.0	33.0	35.0	25.0	35.0
100-101	31.496239963028344	35.0	33.0	35.0	25.0	35.0
102-103	30.803670002934	34.0	31.5	35.0	23.5	35.0
104-105	31.328836802692095	35.0	33.0	35.0	24.0	35.0
106-107	31.423754125426015	35.0	33.0	35.0	24.5	35.0
108-109	31.411741875571217	35.0	33.0	35.0	24.5	35.0
110-111	31.344447420176444	35.0	33.0	35.0	24.0	35.0
112-113	31.304150384059948	35.0	33.0	35.0	24.0	35.0
114-115	31.17870205102328	35.0	33.0	35.0	23.0	35.0
116-117	31.362827101442885	35.0	33.5	35.0	24.0	35.0
118-119	31.343707660459543	35.0	34.0	35.0	24.0	35.0
120-121	31.256650080589715	35.0	34.0	35.0	23.5	35.0
122-123	31.121857586184184	35.0	34.0	35.0	21.5	35.0
124-125	30.484354544677622	34.5	32.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24212.0
3	6432.0
4	3463.0
5	2927.0
6	3083.0
7	3477.0
8	3923.0
9	4302.0
10	4651.0
11	4922.0
12	5608.0
13	7050.0
14	9365.0
15	8552.0
16	5137.0
17	4448.0
18	4052.0
19	3738.0
20	3634.0
21	3685.0
22	4203.0
23	5017.0
24	6599.0
25	8572.0
26	10527.0
27	12932.0
28	15437.0
29	18819.0
30	22848.0
31	28962.0
32	37861.0
33	51326.0
34	79633.0
35	142817.0
36	333541.0
37	689961.0
38	250979.0
39	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.160760594315036	17.505658341268894	11.157590180919916	27.175990883496155
2	16.304072098671885	19.46845955282362	37.093407975229695	27.134060373274803
3	19.058587145622504	22.952580867358748	28.510777323107334	29.47805466391142
4	13.76861301291433	14.960260249562143	33.55298887854075	37.71813785898278
5	15.008727808878952	36.84948422624136	32.54732236486053	15.594465600019165
6	35.185226544153736	33.17332859783231	16.357578989627836	15.283865868386117
7	30.910975362675618	31.320762321385388	18.866266973698256	18.901995342240735
8	28.552943431738175	34.12454036108885	18.3234725175142	18.99904368965878
9	28.184476487116378	15.132974668380129	18.184912112565616	38.49763673193788
10-11	27.225567069382816	24.629277548418127	26.023845959643417	22.121309422555647
12-13	27.101294717480783	22.07397716447558	26.49565713271086	24.32907098533278
14-15	24.531058641737435	23.486542948266326	23.869691806050263	28.11270660394598
16-17	24.650913274649568	26.654112969657362	24.56949124849376	24.125482507199315
18-19	24.584463964913255	25.718635150022084	26.40221459482684	23.294686290237824
20-21	25.32702611907589	24.563425235499643	26.33920768887749	23.770340956546974
22-23	25.28062226410134	24.200456877895803	26.106464579987243	24.412456278015615
24-25	24.68808476875989	24.700360234567547	26.101233126337952	24.510321870334607
26-27	24.340816172666084	25.024394054410244	26.365475057368553	24.26931471555512
28-29	24.43048572567071	25.256804013491536	26.25511430568469	24.05759595515306
30-31	24.901138435585317	24.800895571867436	26.184127333205048	24.113838659342196
32-33	24.644295886592772	24.74484463123054	26.113331010604707	24.49752847157198
34-35	24.729689601327376	25.02685752406698	26.14446258686337	24.09899028774227
36-37	24.665970226929026	25.061269639763918	26.180046184951024	24.092713948356035
38-39	24.616857299008625	25.134564006088038	26.27348610049623	23.9750925944071
40-41	24.69683991694177	24.887256539039083	26.223003558199686	24.19289998581946
42-43	24.613149382639435	24.776457882193604	26.040356796409824	24.570035938757133
44-45	24.51987393518152	24.92872177143988	26.031530358197084	24.51987393518152
46-47	24.48277138511553	25.057267221265956	25.896106808811314	24.563854584807203
48-49	24.41658517280826	25.27407384826928	25.861809460327844	24.447531518594616
50-51	24.47926107738862	25.307680290116835	25.898300157915966	24.314758474578575
52-53	24.675182226465697	25.1411964224524	25.75010131630911	24.43352003477279
54-55	24.445199907130714	24.96411930274	25.778875829787907	24.811804960341384
56-57	24.454416246749577	25.266062407019007	25.593731101009272	24.685790245222144
58-59	24.38794503964058	25.291040975858564	25.86494414065445	24.45606984384641
60-61	24.61356483833996	25.13621477120899	26.009420926301267	24.240799464149777
62-63	24.514218199742032	25.115802956292498	25.929437970196474	24.44054087376899
64-65	24.412233960033376	24.93017463010653	25.859664478959303	24.797926930900783
66-67	24.364287123794746	25.281972768285467	25.65230231149761	24.70143779642218
68-69	24.288488106630435	25.223834205916585	25.805574516867587	24.6821031705854
70-71	24.192537843962413	25.084604233994874	26.010310911374173	24.712547010668544
72-73	24.34472658030847	25.272578663974656	25.739607525917396	24.643087229799484
74-75	24.20935792721564	25.282554921425728	25.735769640302497	24.77231751105614
76-77	24.2718142716428	25.00415748413828	25.66668762419718	25.057340620021733
78-79	24.130596487847416	25.17913632977578	25.771381026994767	24.918886155382037
80-81	24.175118924947867	25.364041798873536	25.64941268304154	24.811426593137053
82-83	24.21833576667766	25.31717853997163	25.72924117762978	24.73524451572093
84-85	24.192496561064445	25.23999061539391	25.589926463663776	24.977586359877872
86-87	24.035658704587654	25.42949883048289	25.629081538504252	24.90576092642521
88-89	23.96351343002227	25.22030451381678	25.63547887262225	25.180703183538693
90-91	24.064032416846505	25.112169136769186	25.769465989174606	25.054332457209703
92-93	23.989308049772383	25.240993466272343	25.81031994743841	24.95937853651686
94-95	24.038994644319622	25.04028940325413	25.756829737703125	25.163886214723124
96-97	23.900173211355956	24.978634362912082	25.814588729177352	25.306603696554607
98-99	23.81792874748513	25.15089228946933	25.650464434709146	25.380714528336394
100-101	23.750423905724453	25.17363124039578	25.83568624825335	25.24025860562642
102-103	23.5911006886806	25.016282779555528	25.990582252600415	25.40203427916346
104-105	23.71411634640351	25.061115910386196	25.888895602430594	25.335872140779703
106-107	23.795576930142932	24.91591921385792	25.86348660708818	25.42501724891097
108-109	23.69545934196699	25.03269533985598	25.803747396280585	25.468097921896447
110-111	23.47987188378091	25.394457079215844	25.696348883718855	25.429322153284396
112-113	23.416580637768757	25.37338537616697	25.820372749855547	25.389661236208728
114-115	23.39774522980181	25.34836477176045	25.79075088060801	25.46313911782973
116-117	23.25138509809728	25.218039317298242	25.89205325483083	25.63852232977365
118-119	23.383427438396225	25.16060461312136	25.782528166876922	25.673439781605488
120-121	23.238879421632756	25.26758494061213	25.852114291873285	25.641421345881827
122-123	23.062813986921487	25.37466134729478	25.784174596460975	25.77835006932276
124-125	22.950403382912693	25.256184251037407	25.816315687658197	25.977096678391696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	6.5
2	11.0
3	34.0
4	38.5
5	55.0
6	140.0
7	383.5
8	684.5
9	785.0
10	672.5
11	524.5
12	492.0
13	529.5
14	578.5
15	617.0
16	685.5
17	745.5
18	790.5
19	876.5
20	957.0
21	1012.5
22	1113.0
23	1259.0
24	1425.5
25	1743.5
26	2267.0
27	3070.5
28	4057.0
29	5349.5
30	7110.5
31	9088.0
32	11738.5
33	14500.5
34	16962.5
35	20042.5
36	23782.5
37	27613.0
38	31536.5
39	35664.0
40	39743.0
41	43709.0
42	47695.5
43	53137.0
44	59031.0
45	64195.0
46	68822.0
47	73482.0
48	78678.0
49	83036.0
50	84894.0
51	84944.5
52	84780.0
53	83884.5
54	82837.0
55	80528.0
56	75235.5
57	68834.5
58	63024.0
59	56590.5
60	48679.0
61	40943.5
62	34120.5
63	26608.0
64	19599.0
65	14823.5
66	11264.5
67	8625.0
68	6743.0
69	5110.5
70	3889.0
71	2991.5
72	2217.5
73	1705.0
74	1286.0
75	952.0
76	658.5
77	380.5
78	199.5
79	105.0
80	54.0
81	25.5
82	23.5
83	15.0
84	7.5
85	4.5
86	4.5
87	5.0
88	4.5
89	2.0
90	1.0
91	1.5
92	1.0
93	1.0
94	1.0
95	1.5
96	1.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022535726475069445
2	0.002449535486420592
3	0.032007263689229064
4	0.1715219181713619
5	0.02313450181619448
6	0.07098209498427671
7	0.0548151607739008
8	0.04675891072967307
9	0.03500114039485423
10-11	0.06393287619557746
12-13	0.05900658816177603
14-15	0.06055796063650907
16-17	0.05282831532380409
18-19	0.03919256778272947
20-21	0.024821959595728665
22-23	0.002694489035062651
24-25	0.004654117424199125
26-27	0.0031299620104263117
28-29	0.00430029563171615
30-31	0.002422318425460363
32-33	0.008872761873034589
34-35	0.004055342083074091
36-37	0.006586528752375369
38-39	0.002122930754897846
40-41	0.0031843961323467693
42-43	0.0037559544125115745
44-45	0.004980722155721869
46-47	0.004409163875557066
48-49	0.001769108962414872
50-51	0.001633023657613728
52-53	7.892947678466351E-4
54-55	0.005035156277642327
56-57	7.620777068864064E-4
58-59	7.348606459261776E-4
60-61	1.36085304801144E-4
62-63	0.0010070312555284654
64-65	0.0
66-67	0.0038376055953922607
68-69	4.899070972841184E-4
70-71	0.0
72-73	0.002422318425460363
74-75	0.005171241582443472
76-77	0.0018779772062557873
78-79	2.449535486420592E-4
80-81	0.0
82-83	0.005715582801648047
84-85	0.001741891901454643
86-87	0.0
88-89	0.0012792018651307535
90-91	7.348606459261776E-4
92-93	0.0
94-95	0.003347698498108142
96-97	0.0011431165603296095
98-99	5.987753411250336E-4
100-101	0.0
102-103	0.001061465377448923
104-105	0.0
106-107	5.715582801648048E-4
108-109	0.002585620791221736
110-111	5.44341219204576E-5
112-113	3.5382179248297436E-4
114-115	0.0
116-117	0.0015785895356932702
118-119	0.002204581937778533
120-121	0.002694489035062651
122-123	0.0013064189260909823
124-125	0.10767069315866513
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1837083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.01710657258154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7939630000547	50.914017767956565
2	10.613275653360704	13.376358458640327
3	3.4100150238171194	6.446678405099626
4	1.6131135959262881	4.066150055526686
5	0.9140036920195096	2.8798934033863213
6	0.5799826691777343	2.1929297804294148
7	0.4052149514910039	1.7874831648038436
8	0.27829245897762983	1.402974843659126
9	0.22031923408181228	1.2495492588710793
>10	1.1118944129596313	12.070078845842112
>50	0.047532283177687644	2.0035612760966144
>100	0.011959869546486018	1.2073782262334116
>500	1.7326216386629373E-4	0.08467281430275375
>1k	2.5989324579944056E-4	0.3182736991521949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2471	0.13450671526545072	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2103	0.11447495839872232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.44341219204576E-5	0.0
4	0.0	0.0	0.0	5.44341219204576E-5	0.0
5	0.0	0.0	0.0	5.44341219204576E-5	0.0
6	0.0	0.0	0.0	5.44341219204576E-5	0.0
7	0.0	0.0	0.0	5.44341219204576E-5	0.0
8	0.0	0.0	0.0	5.44341219204576E-5	0.0
9	0.0	0.0	0.0	1.088682438409152E-4	0.0
10-11	0.0	0.0	0.0	1.088682438409152E-4	0.0
12-13	0.0	0.0	0.0	1.088682438409152E-4	1.9051942672160157E-4
14-15	0.0	0.0	0.0	1.088682438409152E-4	3.2660473152274557E-4
16-17	0.0	0.0	0.0	1.088682438409152E-4	4.354729753636608E-4
18-19	0.0	0.0	0.0	1.6330236576137278E-4	4.899070972841184E-4
20-21	0.0	0.0	0.0	2.993876705625168E-4	5.44341219204576E-4
22-23	0.0	0.0	0.0	5.171241582443472E-4	5.44341219204576E-4
24-25	0.0	0.0	0.0	6.532094630454911E-4	5.987753411250336E-4
26-27	0.0	0.0	0.0	0.0012519848041705247	6.804265240057199E-4
28-29	0.0	0.0	0.0	0.004055342083074091	7.892947678466351E-4
30-31	0.0	0.0	0.0	0.016466321880938422	8.16511828806864E-4
32-33	0.0	0.0	0.0	0.041995925061633035	8.709459507273216E-4
34-35	0.0	0.0	0.0	0.07193469211788472	8.709459507273216E-4
36-37	0.0	0.0	0.0	0.11028353101084709	9.253800726477791E-4
38-39	0.0	0.0	0.0	0.1691812509287822	9.253800726477791E-4
40-41	0.0	0.0	0.0	0.23621687207382575	9.253800726477791E-4
42-43	0.0	0.0	0.0	0.3060014163758524	9.253800726477791E-4
44-45	0.0	0.0	0.0	0.3901565688648798	9.253800726477791E-4
46-47	0.0	0.0	0.0	0.47942852881443027	9.253800726477791E-4
48-49	0.0	0.0	0.0	0.5640191542788213	9.798141945682368E-4
50-51	0.0	0.0	0.0	0.6480654385240079	9.798141945682368E-4
52-53	0.0	0.0	0.0	0.7407939652155073	0.001088682438409152
54-55	0.0	0.0	0.0	0.8490362166543373	0.0011431165603296095
56-57	0.0	0.0	0.0	0.9790521168613503	0.0011431165603296095
58-59	0.0	0.0	0.0	1.1039784266688004	0.0011431165603296095
60-61	0.0	0.0	0.0	1.2231347195526823	0.0011431165603296095
62-63	0.0	0.0	0.0	1.3470267810436436	0.0011431165603296095
64-65	0.0	0.0	0.0	1.4890726221950779	0.0011431165603296095
66-67	0.0	0.0	0.0	1.6572196248073712	0.0011703336212898383
68-69	0.0	0.0	0.0	1.839655584423785	0.0011975506822500671
70-71	0.0	0.0	0.0	2.0175462948598404	0.0011975506822500671
72-73	0.0	0.0	0.0	2.1901568954696113	0.0013064189260909823
74-75	0.0	0.0	0.0	2.3799142444843264	0.0013608530480114399
76-77	0.0	0.0	0.0	2.614171488169016	0.0013608530480114399
78-79	0.0	0.0	0.0	2.8701207294390074	0.0013608530480114399
80-81	2.72170609602288E-5	0.0	0.0	3.113005781448089	0.0013880701089716686
82-83	5.44341219204576E-5	0.0	0.0	3.3515633207644946	0.0015241554137728126
84-85	5.44341219204576E-5	0.0	0.0	3.619243115308345	0.0015241554137728126
86-87	5.44341219204576E-5	0.0	0.0	3.9365668290436524	0.0016058065966534992
88-89	5.44341219204576E-5	0.0	0.0	4.287421961881962	0.0016874577795341855
90-91	5.44341219204576E-5	0.0	0.0	4.638984738305237	0.0016874577795341855
92-93	5.44341219204576E-5	0.0	0.0	4.967276927607517	0.001741891901454643
94-95	5.44341219204576E-5	0.0	0.0	5.318839704030792	0.0017963260233751007
96-97	1.088682438409152E-4	0.0	0.0	5.723176361655952	0.001877977206255787
98-99	1.088682438409152E-4	0.0	0.0	6.197188695339296	0.0019596283891364736
100-101	1.088682438409152E-4	0.0	0.0	6.680454829749118	0.0019596283891364736
102-103	1.088682438409152E-4	0.0	0.0	7.149078185362338	0.0020684966329773888
104-105	1.088682438409152E-4	0.0	0.0	7.62864279948157	0.0021229307548978464
106-107	1.088682438409152E-4	0.0	0.0	8.16163450426573	0.0021229307548978464
108-109	1.088682438409152E-4	0.0	0.0	8.765581086973206	0.0021501478158580754
110-111	1.088682438409152E-4	0.0	0.0	9.400582336236305	0.0023406672425796767
112-113	1.088682438409152E-4	0.0	0.0	10.008121570990532	0.0023951013645001342
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2600	0.0	59.214943	1
GTATCAA	4390	0.0	49.886894	1
TCAACGC	5640	0.0	38.609303	4
CAACGCA	5735	0.0	37.81101	5
TATCAAC	5840	0.0	37.396374	2
GTACATG	9430	0.0	37.14594	1
ATCAACG	5915	0.0	37.021057	3
AACGCAG	6030	0.0	36.2754	6
TACATGG	9885	0.0	35.19274	2
ACATGGG	9830	0.0	34.90212	3
CATGGGG	9010	0.0	31.782984	4
ACGCAGA	7235	0.0	30.315231	7
ATGGGGG	5270	0.0	29.616995	5
CGCAGAG	7645	0.0	28.762684	8
GCAGAGT	8540	0.0	25.538994	9
AGAGTAC	7670	0.0	23.074997	10-11
CAGAGTA	8175	0.0	22.670086	10-11
ATGGGGA	3350	0.0	21.695284	5
GAGTACT	3575	0.0	21.649672	12-13
TGGGGGG	3455	0.0	21.046276	6
>>END_MODULE