FastQCFastQC Report
Mon 15 Aug 2016
SRR2921613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936842
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16200.042.622181
GTATCAA26150.041.4278451
GTACATG85800.037.0464551
TACATGG88400.035.4135972
TCAACGC30150.034.9399644
ACATGGG89550.034.6265073
CAACGCA32100.032.815755
CATGGGG80600.031.0873434
AACGCAG34600.030.2671936
ATCAACG34900.030.1845283
TATCAAC35900.029.3437352
ATGGGGG47300.027.1773245
GAGTACA59850.027.051881
AGTACAT60950.026.4625782
ACGCAGA42800.024.6003687
GTGTACG3553.6379788E-1223.4742831
TATACCG1802.1742824E-523.1440665
CGCAGAG47500.022.5413448
GTGGTAT12200.020.9797971
AGAGTAC46650.019.9015585
Produced by FastQC (version 0.11.5)