FastQCFastQC Report
Mon 15 Aug 2016
SRR2921613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936842
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.10620381011977229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23500.047.968971
GTATCAA37800.045.6008031
TCAACGC45600.036.8755344
GTACATG91950.036.8436851
CAACGCA46550.036.339665
TACATGG96450.034.8091932
AACGCAG49450.034.3396266
ACATGGG96050.034.0228733
ATCAACG49700.033.8361173
TATCAAC50750.033.488572
CATGGGG82050.032.485014
ATGGGGG49600.029.7818895
ACGCAGA61650.027.2527647
CGCAGAG67700.024.992698
CCTATAC4650.024.3662363
GAGTACA65000.022.4811861
GCAGAGT75800.022.3219649
CGTATAC1703.876028E-421.0469953
AGTACAT67000.021.005112
AGAGTAC67250.020.8141065
Produced by FastQC (version 0.11.5)